################################## # NO CUT ENZYMES ( 166 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AfaI AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BbeI BbrPI BcgI BciVI BcnI BglI BglII BlnI BsaAI BsaBI BsaHI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoT22I FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI KspI MamI MfeI MflI MluI MluNI MscI MslI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tth111I TthHB8I XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccIII 253 TCCGGA T^CCGGA AcsI 4 GAATTC [GA]^AATT[CT] AflII 387 CTTAAG C^TTAAG AflIII 616 ACGTGT A^C[GA][CT]GT AlwNI 540 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 4 GAATTC [GA]^AATT[CT] BanII 72 GAGCCC G[GA]GC[CT]^C BbsI 200 GAAGAC GAAGAC(2/6) BbvCI 591 GCTGAGG CCTCAGC(-5/-2) BbvI 563 GCTGC GCAGC(8/12) BclI 215 TGATCA T^GATCA BfrI 387 CTTAAG C^TTAAG BlpI 647 GCTGAGC GC^T[ACGT]AGC Bpu1102I 647 GCTGAGC GC^T[ACGT]AGC BpuAI 200 GAAGAC GAAGAC(2/6) BsaWI 253 TCCGGA [AT]^CCGG[AT] BseAI 253 TCCGGA T^CCGGA BsgI 620 GTGCAG GTGCAG(16/14) BsmI 91 GAATGC GAATGC(1/-1) BspEI 253 TCCGGA T^CCGGA CelII 647 GCTGAGC GC^T[ACGT]AGC EarI 80 GAAGAG CTCTTC(1/4) EcoRI 4 GAATTC G^AATTC EcoRV 717 GATATC GAT^ATC EcoT14I 208 CCAAGG C^C[AT][AT]GG FbaI 215 TGATCA T^GATCA Fnu4HI 563 GCTGC GC^[ACGT]GC HindIII 384 AAGCTT A^AGCTT HphI 430 TCACC GGTGA(8/7) ItaI 563 GCTGC GC^[ACGT]GC Ksp632I 80 GAAGAG CTCTTC(1/4) MaeII 616 ACGT A^CGT MroI 253 TCCGGA T^CCGGA MseI 388 TTAA T^TAA NlaIII 425 CATG CATG^ PflMI 539 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 318 CTGCAG CTGCA^G PvuII 645 CAGCTG CAG^CTG SfcI 318 CTGCAG C^T[GA][CT]AG SmlI 387 CTTAAG C^T[CT][GA]AG StyI 208 CCAAGG C^C[AT][AT]GG Tru9I 388 TTAA T^TAA TseI 563 GCTGC G^C[AT]GC Van91I 539 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 281 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### Asp700I 326 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 522 GAAATCCTTC BmyI 72 GAGCCC G[AGT]GC[ACT]^C 786 GGGCAC BsaI 496 GAGACC GGTCTC(1/5) 515 GGTCTC Bsp1286I 72 GAGCCC G[AGT]GC[ACT]^C 786 GGGCAC DpnII 216 GATC ^GATC 279 GATC FauI 580 CCCGC CCCGC(4/6) 588 CCCGC HapII 254 CCGG C^CGG 509 CCGG HinfI 372 GATTC G^A[ACGT]TC 419 GATTC HpaII 254 CCGG C^CGG 509 CCGG MaeIII 409 GTGAC ^GT[ACGT]AC 755 GTCAC MboI 216 GATC ^GATC 279 GATC MspA1I 589 CCGCTG C[AC]G^C[GT]G 645 CAGCTG MspI 254 CCGG C^CGG 509 CCGG NdeII 216 GATC ^GATC 279 GATC Sau3AI 216 GATC ^GATC 279 GATC StuI 65 AGGCCT AGG^CCT 337 AGGCCT TfiI 372 GATTC G^A[AT]TC 419 GATTC Tsp45I 409 GTGAC ^GT[GC]AC 755 GTCAC Tsp509I 5 AATT ^AATT 220 AATT XmnI 326 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 522 GAAATCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AciI AluI BanI BfaI BpmI BsaJI BsiYI BslI BsmAI BsrI BssKI BstF5I BstNI Cac8I DdeI EcoRII FokI HaeIII MaeI MboII MnlI MvaI MwoI NlaIV ScrFI SfaNI TspRI