##################################
# NO CUT ENZYMES ( 166 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AfaI AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BalI BamHI BbeI BbrPI
BcgI BciVI BcnI BglI BglII
BlnI BsaAI BsaBI BsaHI BseRI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CfoI Cfr10I Cfr13I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoT22I FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MflI MluI
MluNI MscI MslI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI Tth111I
TthHB8I XcmI XhoI XhoII XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccIII 253 TCCGGA T^CCGGA
AcsI 4 GAATTC [GA]^AATT[CT]
AflII 387 CTTAAG C^TTAAG
AflIII 616 ACGTGT A^C[GA][CT]GT
AlwNI 540 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 4 GAATTC [GA]^AATT[CT]
BanII 72 GAGCCC G[GA]GC[CT]^C
BbsI 200 GAAGAC GAAGAC(2/6)
BbvCI 591 GCTGAGG CCTCAGC(-5/-2)
BbvI 563 GCTGC GCAGC(8/12)
BclI 215 TGATCA T^GATCA
BfrI 387 CTTAAG C^TTAAG
BlpI 647 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 647 GCTGAGC GC^T[ACGT]AGC
BpuAI 200 GAAGAC GAAGAC(2/6)
BsaWI 253 TCCGGA [AT]^CCGG[AT]
BseAI 253 TCCGGA T^CCGGA
BsgI 620 GTGCAG GTGCAG(16/14)
BsmI 91 GAATGC GAATGC(1/-1)
BspEI 253 TCCGGA T^CCGGA
CelII 647 GCTGAGC GC^T[ACGT]AGC
EarI 80 GAAGAG CTCTTC(1/4)
EcoRI 4 GAATTC G^AATTC
EcoRV 717 GATATC GAT^ATC
EcoT14I 208 CCAAGG C^C[AT][AT]GG
FbaI 215 TGATCA T^GATCA
Fnu4HI 563 GCTGC GC^[ACGT]GC
HindIII 384 AAGCTT A^AGCTT
HphI 430 TCACC GGTGA(8/7)
ItaI 563 GCTGC GC^[ACGT]GC
Ksp632I 80 GAAGAG CTCTTC(1/4)
MaeII 616 ACGT A^CGT
MroI 253 TCCGGA T^CCGGA
MseI 388 TTAA T^TAA
NlaIII 425 CATG CATG^
PflMI 539 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 318 CTGCAG CTGCA^G
PvuII 645 CAGCTG CAG^CTG
SfcI 318 CTGCAG C^T[GA][CT]AG
SmlI 387 CTTAAG C^T[CT][GA]AG
StyI 208 CCAAGG C^C[AT][AT]GG
Tru9I 388 TTAA T^TAA
TseI 563 GCTGC G^C[AT]GC
Van91I 539 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 281 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Asp700I 326 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
522 GAAATCCTTC
BmyI 72 GAGCCC G[AGT]GC[ACT]^C
786 GGGCAC
BsaI 496 GAGACC GGTCTC(1/5)
515 GGTCTC
Bsp1286I 72 GAGCCC G[AGT]GC[ACT]^C
786 GGGCAC
DpnII 216 GATC ^GATC
279 GATC
FauI 580 CCCGC CCCGC(4/6)
588 CCCGC
HapII 254 CCGG C^CGG
509 CCGG
HinfI 372 GATTC G^A[ACGT]TC
419 GATTC
HpaII 254 CCGG C^CGG
509 CCGG
MaeIII 409 GTGAC ^GT[ACGT]AC
755 GTCAC
MboI 216 GATC ^GATC
279 GATC
MspA1I 589 CCGCTG C[AC]G^C[GT]G
645 CAGCTG
MspI 254 CCGG C^CGG
509 CCGG
NdeII 216 GATC ^GATC
279 GATC
Sau3AI 216 GATC ^GATC
279 GATC
StuI 65 AGGCCT AGG^CCT
337 AGGCCT
TfiI 372 GATTC G^A[AT]TC
419 GATTC
Tsp45I 409 GTGAC ^GT[GC]AC
755 GTCAC
Tsp509I 5 AATT ^AATT
220 AATT
XmnI 326 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
522 GAAATCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI AluI BanI BfaI BpmI
BsaJI BsiYI BslI BsmAI BsrI
BssKI BstF5I BstNI Cac8I DdeI
EcoRII FokI HaeIII MaeI MboII
MnlI MvaI MwoI NlaIV ScrFI
SfaNI TspRI