##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AlwNI AocI AscI Asp700I Asp718I
AspI AviII AvrII BamHI BbrPI
BbvCI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsiEI
BsiWI BsmI Bsp1407I BspHI BspMI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I CpoI
DraI DraIII DrdI EagI EclXI
Eco52I Eco81I EcoO65I EcoRI EcoT22I
FbaI FseI FspI HincII HindII
HpaI KpnI KspI MluI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccI 1105 GTAGAC GT^[AC][GT]AC
AccII 333 CGCG CG^CG
AccIII 486 TCCGGA T^CCGGA
AcsI 859 AAATTT [GA]^AATT[CT]
AflIII 1297 ACATGT A^C[GA][CT]GT
AhdI 166 GACGTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 324 GTGCAC G^TGCAC
Aor51HI 359 AGCGCT AGC^GCT
ApaI 742 GGGCCC GGGCC^C
ApaLI 324 GTGCAC G^TGCAC
ApoI 859 AAATTT [GA]^AATT[CT]
AseI 1432 ATTAAT AT^TAAT
AsnI 1432 ATTAAT AT^TAAT
AspEI 166 GACGTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 501 GGCGCC G^G[CT][GA]CC
BbeI 501 GGCGCC GGCGC^C
BciVI 800 GTATCC GTATCC(6/5)
BpmI 778 CTGGAG CTGGAG(16/14)
BsaBI 63 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 781 GAGACC GGTCTC(1/5)
BseAI 486 TCCGGA T^CCGGA
BsgI 982 GTGCAG GTGCAG(16/14)
BspDI 160 ATCGAT AT^CGAT
BspEI 486 TCCGGA T^CCGGA
BspLU11I 1297 ACATGT A^CATGT
BsrBI 1164 CCGCTC CCGCTC(-3/-3)
BstUI 333 CGCG CG^CG
BstYI 68 AGATCC [GA]^GATC[CT]
ClaI 160 ATCGAT AT^CGAT
Eam1105I 166 GACGTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 127 GAAGAG CTCTTC(1/4)
Eco47III 359 AGCGCT AGC^GCT
EcoNI 942 CCTTGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1201 GATATC GAT^ATC
HindIII 1011 AAGCTT A^AGCTT
KasI 501 GGCGCC G^GCGCC
Ksp632I 127 GAAGAG CTCTTC(1/4)
MamI 63 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1415 CAATTG C^AATTG
MflI 68 AGATCC [GA]^GATC[CT]
MroI 486 TCCGGA T^CCGGA
MunI 1415 CAATTG C^AATTG
MvnI 333 CGCG CG^CG
NarI 501 GGCGCC GG^CGCC
PaeR7I 580 CTCGAG C^TCGAG
PshBI 1432 ATTAAT AT^TAAT
PstI 549 CTGCAG CTGCA^G
PvuII 1214 CAGCTG CAG^CTG
SapI 127 GAAGAGC GCTCTTC(1/4)
SfcI 549 CTGCAG C^T[GA][CT]AG
SfoI 501 GGCGCC GGC^GCC
SmaI 435 CCCGGG CCC^GGG
SmlI 580 CTCGAG C^T[CT][GA]AG
XhoI 580 CTCGAG C^TCGAG
XhoII 68 AGATCC [GA]^GATC[CT]
XmaCI 435 CCCGGG C^CCGGG
XmaI 435 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 315 GACGCC G[GA]^CG[CT]C
501 GGCGCC
AspHI 34 GAGCAC G[AT]GC[AT]^C
324 GTGCAC
AvaI 435 CCCGGG C^[CT]CG[GA]G
580 CTCGAG
BbsI 199 GAAGAC GAAGAC(2/6)
289 GAAGAC
BcgI 377 CGAACATCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1182 GCACGAGGGTCG
BpuAI 199 GAAGAC GAAGAC(2/6)
289 GAAGAC
BsaHI 315 GACGCC G[GA]^CG[CT]C
501 GGCGCC
BsaWI 269 ACCGGA [AT]^CCGG[AT]
486 TCCGGA
BsiHKAI 34 GAGCAC G[AT]GC[AT]^C
324 GTGCAC
BsmBI 308 GAGACG CGTCTC(1/5)
313 GAGACG
BsmFI 572 GGGAC GGGAC(10/14)
953 GGGAC
BsoBI 435 CCCGGG C^[CT]CG[GA]G
580 CTCGAG
BssSI 478 CACGAG CACGAG(-5/-1)
1183 CACGAG
EcoT14I 923 CCATGG C^C[AT][AT]GG
1142 CCATGG
FauI 498 GCGGG CCCGC(4/6)
591 GCGGG
HgaI 315 GACGC GACGC(5/10)
415 GACGC
Hin1I 315 GACGCC G[GA]^CG[CT]C
501 GGCGCC
HphI 118 GGTGA GGTGA(8/7)
1029 GGTGA
MseI 1425 TTAA T^TAA
1433 TTAA
NcoI 923 CCATGG C^CATGG
1142 CCATGG
NspI 1073 ACATGC [GA]CATG^[CT]
1297 ACATGT
PleI 45 GACTC GAGTC(4/5)
346 GACTC
PpuMI 213 AGGACCC [GA]G^G[AT]CC[CT]
722 AGGACCC
StyI 923 CCATGG C^C[AT][AT]GG
1142 CCATGG
Tru9I 1425 TTAA T^TAA
1433 TTAA
XcmI 1127 CCAAAGCTCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1142 CCATGGCCAGAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BalI BanII BbvI BcnI BfaI
BmyI BsaJI BseRI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I