################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## AatII Acc65I AclI AflII AgeI AlwNI AocI AscI Asp700I Asp718I AspI AviII AvrII BamHI BbrPI BbvCI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsiEI BsiWI BsmI Bsp1407I BspHI BspMI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I CpoI DraI DraIII DrdI EagI EclXI Eco52I Eco81I EcoO65I EcoRI EcoT22I FbaI FseI FspI HincII HindII HpaI KpnI KspI MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AccI 1105 GTAGAC GT^[AC][GT]AC AccII 333 CGCG CG^CG AccIII 486 TCCGGA T^CCGGA AcsI 859 AAATTT [GA]^AATT[CT] AflIII 1297 ACATGT A^C[GA][CT]GT AhdI 166 GACGTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 324 GTGCAC G^TGCAC Aor51HI 359 AGCGCT AGC^GCT ApaI 742 GGGCCC GGGCC^C ApaLI 324 GTGCAC G^TGCAC ApoI 859 AAATTT [GA]^AATT[CT] AseI 1432 ATTAAT AT^TAAT AsnI 1432 ATTAAT AT^TAAT AspEI 166 GACGTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BanI 501 GGCGCC G^G[CT][GA]CC BbeI 501 GGCGCC GGCGC^C BciVI 800 GTATCC GTATCC(6/5) BpmI 778 CTGGAG CTGGAG(16/14) BsaBI 63 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 781 GAGACC GGTCTC(1/5) BseAI 486 TCCGGA T^CCGGA BsgI 982 GTGCAG GTGCAG(16/14) BspDI 160 ATCGAT AT^CGAT BspEI 486 TCCGGA T^CCGGA BspLU11I 1297 ACATGT A^CATGT BsrBI 1164 CCGCTC CCGCTC(-3/-3) BstUI 333 CGCG CG^CG BstYI 68 AGATCC [GA]^GATC[CT] ClaI 160 ATCGAT AT^CGAT Eam1105I 166 GACGTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 127 GAAGAG CTCTTC(1/4) Eco47III 359 AGCGCT AGC^GCT EcoNI 942 CCTTGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 1201 GATATC GAT^ATC HindIII 1011 AAGCTT A^AGCTT KasI 501 GGCGCC G^GCGCC Ksp632I 127 GAAGAG CTCTTC(1/4) MamI 63 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1415 CAATTG C^AATTG MflI 68 AGATCC [GA]^GATC[CT] MroI 486 TCCGGA T^CCGGA MunI 1415 CAATTG C^AATTG MvnI 333 CGCG CG^CG NarI 501 GGCGCC GG^CGCC PaeR7I 580 CTCGAG C^TCGAG PshBI 1432 ATTAAT AT^TAAT PstI 549 CTGCAG CTGCA^G PvuII 1214 CAGCTG CAG^CTG SapI 127 GAAGAGC GCTCTTC(1/4) SfcI 549 CTGCAG C^T[GA][CT]AG SfoI 501 GGCGCC GGC^GCC SmaI 435 CCCGGG CCC^GGG SmlI 580 CTCGAG C^T[CT][GA]AG XhoI 580 CTCGAG C^TCGAG XhoII 68 AGATCC [GA]^GATC[CT] XmaCI 435 CCCGGG C^CCGGG XmaI 435 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcyI 315 GACGCC G[GA]^CG[CT]C 501 GGCGCC AspHI 34 GAGCAC G[AT]GC[AT]^C 324 GTGCAC AvaI 435 CCCGGG C^[CT]CG[GA]G 580 CTCGAG BbsI 199 GAAGAC GAAGAC(2/6) 289 GAAGAC BcgI 377 CGAACATCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1182 GCACGAGGGTCG BpuAI 199 GAAGAC GAAGAC(2/6) 289 GAAGAC BsaHI 315 GACGCC G[GA]^CG[CT]C 501 GGCGCC BsaWI 269 ACCGGA [AT]^CCGG[AT] 486 TCCGGA BsiHKAI 34 GAGCAC G[AT]GC[AT]^C 324 GTGCAC BsmBI 308 GAGACG CGTCTC(1/5) 313 GAGACG BsmFI 572 GGGAC GGGAC(10/14) 953 GGGAC BsoBI 435 CCCGGG C^[CT]CG[GA]G 580 CTCGAG BssSI 478 CACGAG CACGAG(-5/-1) 1183 CACGAG EcoT14I 923 CCATGG C^C[AT][AT]GG 1142 CCATGG FauI 498 GCGGG CCCGC(4/6) 591 GCGGG HgaI 315 GACGC GACGC(5/10) 415 GACGC Hin1I 315 GACGCC G[GA]^CG[CT]C 501 GGCGCC HphI 118 GGTGA GGTGA(8/7) 1029 GGTGA MseI 1425 TTAA T^TAA 1433 TTAA NcoI 923 CCATGG C^CATGG 1142 CCATGG NspI 1073 ACATGC [GA]CATG^[CT] 1297 ACATGT PleI 45 GACTC GAGTC(4/5) 346 GACTC PpuMI 213 AGGACCC [GA]G^G[AT]CC[CT] 722 AGGACCC StyI 923 CCATGG C^C[AT][AT]GG 1142 CCATGG Tru9I 1425 TTAA T^TAA 1433 TTAA XcmI 1127 CCAAAGCTCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1142 CCATGGCCAGAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BalI BanII BbvI BcnI BfaI BmyI BsaJI BseRI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I