################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflIII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspI AvaI AviII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BciVI BclI BcnI BglI BglII BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspHI BspLU11I BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraIII DsaI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FbaI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MroI MslI MspA1I MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI Tth111I TthHB8I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AciI 806 GCGG CCGC(-3/-1) AcsI 913 GAATTT [GA]^AATT[CT] AflII 422 CTTAAG C^TTAAG AhdI 109 GACCATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 786 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 913 GAATTT [GA]^AATT[CT] AspEI 109 GACCATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 994 GTGCTC G[AT]GC[AT]^C AvrII 1081 CCTAGG C^CTAGG BalI 653 TGGCCA TGG^CCA BcgI 829 CGAGGCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 422 CTTAAG C^TTAAG BlnI 1081 CCTAGG C^CTAGG BlpI 996 GCTCAGC GC^T[ACGT]AGC BmyI 994 GTGCTC G[AGT]GC[ACT]^C Bpu1102I 996 GCTCAGC GC^T[ACGT]AGC BseRI 954 GAGGAG GAGGAG(10/8) BsgI 448 GTGCAG GTGCAG(16/14) BsiHKAI 994 GTGCTC G[AT]GC[AT]^C BsmI 54 GCATTC GAATGC(1/-1) Bsp1286I 994 GTGCTC G[AGT]GC[ACT]^C Bsp1407I 239 TGTACA T^GTACA BsrBI 804 GAGCGG CCGCTC(-3/-3) BsrFI 985 ACCGGC [GA]^CCGG[CT] BsrGI 239 TGTACA T^GTACA BstF5I 290 GGATG GGATG(2/0) BstYI 492 GGATCT [GA]^GATC[CT] CelII 996 GCTCAGC GC^T[ACGT]AGC CfoI 743 GCGC GCG^C Cfr10I 985 ACCGGC [GA]^CCGG[CT] DrdI 356 GACTTCTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 109 GACCATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT22I 608 ATGCAT ATGCA^T FauI 806 GCGGG CCCGC(4/6) FokI 290 GGATG GGATG(9/13) HapII 986 CCGG C^CGG HgaI 752 GCGTC GACGC(5/10) HhaI 743 GCGC GCG^C HinP1I 743 GCGC G^CGC HpaII 986 CCGG C^CGG MflI 492 GGATCT [GA]^GATC[CT] MluNI 653 TGGCCA TGG^CCA MscI 653 TGGCCA TGG^CCA MspI 986 CCGG C^CGG NdeI 141 CATATG CA^TATG NsiI 608 ATGCAT ATGCA^T PflMI 562 CCAAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 624 GGGTCCC [GA]G^G[AT]CC[CT] SexAI 527 ACCTGGT A^CC[AT]GGT SmlI 422 CTTAAG C^T[CT][GA]AG SspBI 239 TGTACA T^GTACA Van91I 562 CCAAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 118 TCTAGA T^CTAGA XcmI 1021 CCAATCATCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 492 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AfaI 219 GTAC GT^AC 240 GTAC Asp700I 696 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 891 GAAATGATTC AvaII 625 GGTCC G^G[AT]CC 904 GGACC BbvI 923 GCAGC GCAGC(8/12) 989 GCTGC BpmI 783 CTCCAG CTGGAG(16/14) 569 CTGGAG BsmAI 1120 GAGAC GTCTC(1/5) 456 GTCTC BsmFI 1051 GGGAC GGGAC(10/14) 626 GTCCC Cac8I 599 GCAGGC GC[ACGT]^[ACGT]GC 992 GCGTGC DraII 624 GGGTCCC [GA]G^G[ACGT]CC[CT] 831 AGGCCCC EaeI 653 TGGCCA [CT]^GGCC[GA] 669 CGGCCA EcoO109I 624 GGGTCCC [GA]G^G[ACGT]CC[CT] 831 AGGCCCC EcoT14I 1047 CCTTGG C^C[AT][AT]GG 1081 CCTAGG Fnu4HI 923 GCAGC GC^[ACGT]GC 989 GCTGC HphI 227 GGTGA GGTGA(8/7) 299 GGTGA ItaI 923 GCAGC GC^[ACGT]GC 989 GCTGC PstI 408 CTGCAG CTGCA^G 690 CTGCAG RsaI 219 GTAC GT^AC 240 GTAC SfcI 408 CTGCAG C^T[GA][CT]AG 690 CTGCAG StyI 1047 CCTTGG C^C[AT][AT]GG 1081 CCTAGG TseI 923 GCAGC G^C[AT]GC 989 GCTGC TspRI 347 CAGTG CA[GC]TG[ACGT][ACGT]^ 580 CACTG XmnI 696 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 891 GAAATGATTC ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AluI AlwI BfaI BsaJI BsiYI BslI BspMI BsrI BssKI BstNI Cfr13I DdeI DpnII EarI EcoRII HaeIII HinfI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI TfiI Tru9I Tsp45I Tsp509I