##################################
# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspI AvaI
AviII BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BciVI BclI
BcnI BglI BglII BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BspHI BspLU11I BsrDI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DsaI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV FbaI
FseI FspI HaeII Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeII MamI MfeI MluI
MroI MslI MspA1I MunI MvnI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TaqI Tth111I
TthHB8I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AciI 806 GCGG CCGC(-3/-1)
AcsI 913 GAATTT [GA]^AATT[CT]
AflII 422 CTTAAG C^TTAAG
AhdI 109 GACCATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 786 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 913 GAATTT [GA]^AATT[CT]
AspEI 109 GACCATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 994 GTGCTC G[AT]GC[AT]^C
AvrII 1081 CCTAGG C^CTAGG
BalI 653 TGGCCA TGG^CCA
BcgI 829 CGAGGCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 422 CTTAAG C^TTAAG
BlnI 1081 CCTAGG C^CTAGG
BlpI 996 GCTCAGC GC^T[ACGT]AGC
BmyI 994 GTGCTC G[AGT]GC[ACT]^C
Bpu1102I 996 GCTCAGC GC^T[ACGT]AGC
BseRI 954 GAGGAG GAGGAG(10/8)
BsgI 448 GTGCAG GTGCAG(16/14)
BsiHKAI 994 GTGCTC G[AT]GC[AT]^C
BsmI 54 GCATTC GAATGC(1/-1)
Bsp1286I 994 GTGCTC G[AGT]GC[ACT]^C
Bsp1407I 239 TGTACA T^GTACA
BsrBI 804 GAGCGG CCGCTC(-3/-3)
BsrFI 985 ACCGGC [GA]^CCGG[CT]
BsrGI 239 TGTACA T^GTACA
BstF5I 290 GGATG GGATG(2/0)
BstYI 492 GGATCT [GA]^GATC[CT]
CelII 996 GCTCAGC GC^T[ACGT]AGC
CfoI 743 GCGC GCG^C
Cfr10I 985 ACCGGC [GA]^CCGG[CT]
DrdI 356 GACTTCTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 109 GACCATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 608 ATGCAT ATGCA^T
FauI 806 GCGGG CCCGC(4/6)
FokI 290 GGATG GGATG(9/13)
HapII 986 CCGG C^CGG
HgaI 752 GCGTC GACGC(5/10)
HhaI 743 GCGC GCG^C
HinP1I 743 GCGC G^CGC
HpaII 986 CCGG C^CGG
MflI 492 GGATCT [GA]^GATC[CT]
MluNI 653 TGGCCA TGG^CCA
MscI 653 TGGCCA TGG^CCA
MspI 986 CCGG C^CGG
NdeI 141 CATATG CA^TATG
NsiI 608 ATGCAT ATGCA^T
PflMI 562 CCAAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 624 GGGTCCC [GA]G^G[AT]CC[CT]
SexAI 527 ACCTGGT A^CC[AT]GGT
SmlI 422 CTTAAG C^T[CT][GA]AG
SspBI 239 TGTACA T^GTACA
Van91I 562 CCAAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 118 TCTAGA T^CTAGA
XcmI 1021 CCAATCATCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 492 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 219 GTAC GT^AC
240 GTAC
Asp700I 696 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
891 GAAATGATTC
AvaII 625 GGTCC G^G[AT]CC
904 GGACC
BbvI 923 GCAGC GCAGC(8/12)
989 GCTGC
BpmI 783 CTCCAG CTGGAG(16/14)
569 CTGGAG
BsmAI 1120 GAGAC GTCTC(1/5)
456 GTCTC
BsmFI 1051 GGGAC GGGAC(10/14)
626 GTCCC
Cac8I 599 GCAGGC GC[ACGT]^[ACGT]GC
992 GCGTGC
DraII 624 GGGTCCC [GA]G^G[ACGT]CC[CT]
831 AGGCCCC
EaeI 653 TGGCCA [CT]^GGCC[GA]
669 CGGCCA
EcoO109I 624 GGGTCCC [GA]G^G[ACGT]CC[CT]
831 AGGCCCC
EcoT14I 1047 CCTTGG C^C[AT][AT]GG
1081 CCTAGG
Fnu4HI 923 GCAGC GC^[ACGT]GC
989 GCTGC
HphI 227 GGTGA GGTGA(8/7)
299 GGTGA
ItaI 923 GCAGC GC^[ACGT]GC
989 GCTGC
PstI 408 CTGCAG CTGCA^G
690 CTGCAG
RsaI 219 GTAC GT^AC
240 GTAC
SfcI 408 CTGCAG C^T[GA][CT]AG
690 CTGCAG
StyI 1047 CCTTGG C^C[AT][AT]GG
1081 CCTAGG
TseI 923 GCAGC G^C[AT]GC
989 GCTGC
TspRI 347 CAGTG CA[GC]TG[ACGT][ACGT]^
580 CACTG
XmnI 696 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
891 GAAATGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AluI AlwI BfaI BsaJI BsiYI
BslI BspMI BsrI BssKI BstNI
Cfr13I DdeI DpnII EarI EcoRII
HaeIII HinfI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI TfiI Tru9I
Tsp45I Tsp509I