################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BamHI BbeI BbrPI BbsI BcgI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI Bst1107I BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI HincII HindII HindIII HinfI HpaI KasI KpnI KspI MaeII MamI MfeI MflI MluI MroI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI SfiI SfoI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TfiI Tth111I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AfaI 878 GTAC GT^AC ApaI 44 GGGCCC GGGCC^C AspHI 473 GAGCAC G[AT]GC[AT]^C BalI 143 TGGCCA TGG^CCA BanII 44 GGGCCC G[GA]GC[CT]^C BciVI 762 GTATCC GTATCC(6/5) BclI 691 TGATCA T^GATCA BglI 940 GCCAGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaI 273 GAGACC GGTCTC(1/5) BsiEI 314 CGGTCG CG[GA][CT]^CG BsiHKAI 473 GAGCAC G[AT]GC[AT]^C BsmAI 273 GAGAC GTCTC(1/5) BssSI 200 CTCGTG CACGAG(-5/-1) BstAPI 428 GCATCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 944 GGATG GGATG(2/0) BstXI 766 CCAAATATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EaeI 143 TGGCCA [CT]^GGCC[GA] EcoNI 519 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 691 TGATCA T^GATCA FokI 944 GGATG GGATG(9/13) HaeII 727 GGCGCT [GA]GCGC^[CT] MluNI 143 TGGCCA TGG^CCA MscI 143 TGGCCA TGG^CCA NlaIII 828 CATG CATG^ PstI 591 CTGCAG CTGCA^G RsaI 878 GTAC GT^AC SapI 362 GCTCTTC GCTCTTC(1/4) ScaI 877 AGTACT AGT^ACT SexAI 265 ACCTGGT A^CC[AT]GGT SfaNI 428 GCATC GCATC(5/9) SmlI 662 CTTGAG C^T[CT][GA]AG TaqI 981 TCGA T^CGA Tsp45I 15 GTGAC ^GT[GC]AC TthHB8I 981 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AcyI 194 GACGCC G[GA]^CG[CT]C 411 GACGCC AgeI 5 ACCGGT A^CCGGT 312 ACCGGT AvaI 59 CCCGGG C^[CT]CG[GA]G 231 CCCGGG BanI 227 GGTGCC G^G[CT][GA]CC 418 GGCACC BbvCI 971 CCTCAGC CCTCAGC(-5/-2) 294 GCTGAGG BsaHI 194 GACGCC G[GA]^CG[CT]C 411 GACGCC BsoBI 59 CCCGGG C^[CT]CG[GA]G 231 CCCGGG BsrI 782 CCAGT ACTGG(1/-1) 1017 CCAGT BssHII 301 GCGCGC G^CGCGC 303 GCGCGC DpnII 692 GATC ^GATC 863 GATC EarI 363 CTCTTC CTCTTC(1/4) 630 GAAGAG Hin1I 194 GACGCC G[GA]^CG[CT]C 411 GACGCC HphI 14 GGTGA GGTGA(8/7) 470 GGTGA Ksp632I 363 CTCTTC CTCTTC(1/4) 630 GAAGAG MboI 692 GATC ^GATC 863 GATC MboII 630 GAAGA GAAGA(8/7) 364 TCTTC MseI 795 TTAA T^TAA 932 TTAA MspA1I 807 CAGCTG C[AC]G^C[GT]G 891 CAGCTG NdeII 692 GATC ^GATC 863 GATC PinAI 5 ACCGGT A^CCGGT 312 ACCGGT PpuMI 496 GGGACCC [GA]G^G[AT]CC[CT] 639 AGGACCT PvuII 807 CAGCTG CAG^CTG 891 CAGCTG Sau3AI 692 GATC ^GATC 863 GATC SfcI 591 CTGCAG C^T[GA][CT]AG 855 CTGTAG SgrAI 4 CACCGGTG C[GA]^CCGG[CT]G 159 CGCCGGTG SmaI 59 CCCGGG CCC^GGG 231 CCCGGG Tru9I 795 TTAA T^TAA 932 TTAA Tsp509I 793 AATT ^AATT 935 AATT XmaCI 59 CCCGGG C^CCGGG 231 CCCGGG XmaI 59 CCCGGG C^CCGGG 231 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AccII AciI AluI AvaII AviII BbvI BcnI BfaI BmyI BpmI BsaJI BsaWI BseRI BsgI BsiYI BslI BsmFI Bsp1286I BsrBI BsrFI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DraII EcoO109I EcoRII FauI Fnu4HI FspI HaeIII HapII HgaI HhaI HinP1I HpaII ItaI MaeI MaeIII MnlI MspI MvaI MvnI MwoI NciI NlaIV Sau96I ScrFI StuI TseI TspRI