##################################
# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BamHI
BbeI BbrPI BbsI BcgI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrGI Bst1107I
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FseI
HincII HindII HindIII HinfI HpaI
KasI KpnI KspI MaeII MamI
MfeI MflI MluI MroI MslI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
SfiI SfoI SfuI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI TfiI Tth111I Van91I XbaI
XcmI XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AfaI 878 GTAC GT^AC
ApaI 44 GGGCCC GGGCC^C
AspHI 473 GAGCAC G[AT]GC[AT]^C
BalI 143 TGGCCA TGG^CCA
BanII 44 GGGCCC G[GA]GC[CT]^C
BciVI 762 GTATCC GTATCC(6/5)
BclI 691 TGATCA T^GATCA
BglI 940 GCCAGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 273 GAGACC GGTCTC(1/5)
BsiEI 314 CGGTCG CG[GA][CT]^CG
BsiHKAI 473 GAGCAC G[AT]GC[AT]^C
BsmAI 273 GAGAC GTCTC(1/5)
BssSI 200 CTCGTG CACGAG(-5/-1)
BstAPI 428 GCATCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 944 GGATG GGATG(2/0)
BstXI 766 CCAAATATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI 143 TGGCCA [CT]^GGCC[GA]
EcoNI 519 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 691 TGATCA T^GATCA
FokI 944 GGATG GGATG(9/13)
HaeII 727 GGCGCT [GA]GCGC^[CT]
MluNI 143 TGGCCA TGG^CCA
MscI 143 TGGCCA TGG^CCA
NlaIII 828 CATG CATG^
PstI 591 CTGCAG CTGCA^G
RsaI 878 GTAC GT^AC
SapI 362 GCTCTTC GCTCTTC(1/4)
ScaI 877 AGTACT AGT^ACT
SexAI 265 ACCTGGT A^CC[AT]GGT
SfaNI 428 GCATC GCATC(5/9)
SmlI 662 CTTGAG C^T[CT][GA]AG
TaqI 981 TCGA T^CGA
Tsp45I 15 GTGAC ^GT[GC]AC
TthHB8I 981 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 194 GACGCC G[GA]^CG[CT]C
411 GACGCC
AgeI 5 ACCGGT A^CCGGT
312 ACCGGT
AvaI 59 CCCGGG C^[CT]CG[GA]G
231 CCCGGG
BanI 227 GGTGCC G^G[CT][GA]CC
418 GGCACC
BbvCI 971 CCTCAGC CCTCAGC(-5/-2)
294 GCTGAGG
BsaHI 194 GACGCC G[GA]^CG[CT]C
411 GACGCC
BsoBI 59 CCCGGG C^[CT]CG[GA]G
231 CCCGGG
BsrI 782 CCAGT ACTGG(1/-1)
1017 CCAGT
BssHII 301 GCGCGC G^CGCGC
303 GCGCGC
DpnII 692 GATC ^GATC
863 GATC
EarI 363 CTCTTC CTCTTC(1/4)
630 GAAGAG
Hin1I 194 GACGCC G[GA]^CG[CT]C
411 GACGCC
HphI 14 GGTGA GGTGA(8/7)
470 GGTGA
Ksp632I 363 CTCTTC CTCTTC(1/4)
630 GAAGAG
MboI 692 GATC ^GATC
863 GATC
MboII 630 GAAGA GAAGA(8/7)
364 TCTTC
MseI 795 TTAA T^TAA
932 TTAA
MspA1I 807 CAGCTG C[AC]G^C[GT]G
891 CAGCTG
NdeII 692 GATC ^GATC
863 GATC
PinAI 5 ACCGGT A^CCGGT
312 ACCGGT
PpuMI 496 GGGACCC [GA]G^G[AT]CC[CT]
639 AGGACCT
PvuII 807 CAGCTG CAG^CTG
891 CAGCTG
Sau3AI 692 GATC ^GATC
863 GATC
SfcI 591 CTGCAG C^T[GA][CT]AG
855 CTGTAG
SgrAI 4 CACCGGTG C[GA]^CCGG[CT]G
159 CGCCGGTG
SmaI 59 CCCGGG CCC^GGG
231 CCCGGG
Tru9I 795 TTAA T^TAA
932 TTAA
Tsp509I 793 AATT ^AATT
935 AATT
XmaCI 59 CCCGGG C^CCGGG
231 CCCGGG
XmaI 59 CCCGGG C^CCGGG
231 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccII AciI AluI AvaII AviII
BbvI BcnI BfaI BmyI BpmI
BsaJI BsaWI BseRI BsgI BsiYI
BslI BsmFI Bsp1286I BsrBI BsrFI
BssKI BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DraII EcoO109I
EcoRII FauI Fnu4HI FspI HaeIII
HapII HgaI HhaI HinP1I HpaII
ItaI MaeI MaeIII MnlI MspI
MvaI MvnI MwoI NciI NlaIV
Sau96I ScrFI StuI TseI TspRI