##################################
# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AgeI
AlwNI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspI AviII AvrII BalI BbeI
BbsI BciVI BclI BfrI BglII
BlnI BpmI BpuAI BsaBI BsaHI
BsaWI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrDI BsrGI BssHII Bst1107I BstBI
BstZ17I Bsu36I ClaI CpoI DraI
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
MamI MfeI MluI MluNI MroI
MscI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PshAI PshBI Psp1406I PstI
RsaI RsrII SalI SbfI ScaI
SexAI SfcI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tsp509I Tth111I Van91I
XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AflIII 594 ACACGT A^C[GA][CT]GT
AhdI 86 GACCAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 86 GACCAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII 1349 GAGCTC G[GA]GC[CT]^C
BbrPI 933 CACGTG CAC^GTG
BbvCI 490 CCTCAGC CCTCAGC(-5/-2)
BglI 447 GCCCCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1115 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1115 GCTTAGC GC^T[ACGT]AGC
BspHI 830 TCATGA T^CATGA
BstAPI 968 GCACCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 602 GGTGACC G^GT[ACGT]ACC
BstPI 602 GGTGACC G^GT[ACGT]ACC
CelII 1115 GCTTAGC GC^T[ACGT]AGC
DraIII 969 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 871 TGGCCG [CT]^GGCC[GA]
Eam1105I 86 GACCAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 286 CCTACGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 602 GGTGACC G^GT[ACGT]ACC
EcoT14I 193 CCAAGG C^C[AT][AT]GG
KspI 960 CCGCGG CCGC^GG
MseI 1090 TTAA T^TAA
NaeI 365 GCCGGC GCC^GGC
NgoMIV 365 GCCGGC G^CCGGC
NspI 715 ACATGC [GA]CATG^[CT]
PleI 1306 GACTC GAGTC(4/5)
PmaCI 933 CACGTG CAC^GTG
PmlI 933 CACGTG CAC^GTG
PvuI 807 CGATCG CGAT^CG
PvuII 264 CAGCTG CAG^CTG
RcaI 830 TCATGA T^CATGA
SacI 1349 GAGCTC GAGCT^C
SacII 960 CCGCGG CCGC^GG
SapI 180 GAAGAGC GCTCTTC(1/4)
SfiI 446 GGCCCCCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StyI 193 CCAAGG C^C[AT][AT]GG
TaqI 884 TCGA T^CGA
TfiI 1283 GAATC G^A[AT]TC
Tru9I 1090 TTAA T^TAA
TthHB8I 884 TCGA T^CGA
XbaI 989 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccII 921 CGCG CG^CG
961 CGCG
Alw44I 507 GTGCAC G^TGCAC
975 GTGCAC
ApaLI 507 GTGCAC G^TGCAC
975 GTGCAC
BanI 275 GGTGCC G^G[CT][GA]CC
473 GGCACC
BsaI 145 GAGACC GGTCTC(1/5)
727 GAGACC
BsiEI 803 CGACCG CG[GA][CT]^CG
807 CGATCG
BsrBI 1131 CCGCTC CCGCTC(-3/-3)
924 GAGCGG
BsrFI 365 GCCGGC [GA]^CCGG[CT]
772 ACCGGC
BssSI 231 CACGAG CACGAG(-5/-1)
112 CTCGTG
BstUI 921 CGCG CG^CG
961 CGCG
BstXI 535 CCATGACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1073 CCACCAGAATGG
Cfr10I 365 GCCGGC [GA]^CCGG[CT]
772 ACCGGC
HinfI 1283 GAATC G^A[ACGT]TC
1306 GACTC
MvnI 921 CGCG CG^CG
961 CGCG
SmaI 271 CCCGGG CCC^GGG
1157 CCCGGG
StuI 318 AGGCCT AGG^CCT
1028 AGGCCT
XmaCI 271 CCCGGG C^CCGGG
1157 CCCGGG
XmaI 271 CCCGGG C^CCGGG
1157 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaI
AvaII BamHI BbvI BcgI BcnI
BfaI BmyI BsaAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PpuMI Sau3AI Sau96I ScrFI
SfaNI SmlI TseI Tsp45I TspRI
XhoII