##################################
# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AvaI
AviII AvrII BamHI BanII BbeI
BbrPI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BseRI BsgI
BsiHKAI BsiWI BsmI BsoBI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrDI
BsrGI BsrI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstYI
BstZ17I CelII CfoI ClaI CpoI
DraI DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HpaI KasI KpnI Ksp632I KspI
MamI MflI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI TthHB8I Van91I XcmI XhoI
XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccIII 116 TCCGGA T^CCGGA
AflIII 14 ACGCGT A^C[GA][CT]GT
AgeI 155 ACCGGT A^CCGGT
AlwI 543 GGATC GGATC(4/5)
AocI 818 CCTGAGG CC^T[ACGT]AGG
AspI 32 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 218 GGACC G^G[AT]CC
BanI 1156 GGTGCC G^G[CT][GA]CC
BbvCI 1067 GCTGAGG CCTCAGC(-5/-2)
BfaI 1028 CTAG C^TAG
BmyI 1157 GTGCCC G[AGT]GC[ACT]^C
BpmI 591 CTCCAG CTGGAG(16/14)
BsaI 1088 GGTCTC GGTCTC(1/5)
BseAI 116 TCCGGA T^CCGGA
BsiEI 153 CGACCG CG[GA][CT]^CG
BsmBI 375 CGTCTC CGTCTC(1/5)
Bsp1286I 1157 GTGCCC G[AGT]GC[ACT]^C
BspEI 116 TCCGGA T^CCGGA
BsrFI 155 ACCGGT [GA]^CCGG[CT]
BstAPI 167 GCACCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 818 CCTGAGG CC^T[ACGT]AGG
Cfr10I 155 ACCGGT [GA]^CCGG[CT]
DraIII 168 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 263 CCGTGG C^C[GA][CT]GG
Eco81I 818 CCTGAGG CC^T[ACGT]AGG
EcoNI 814 CCTGCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HindIII 73 AAGCTT A^AGCTT
HphI 270 GGTGA GGTGA(8/7)
MaeI 1028 CTAG C^TAG
MaeII 1311 ACGT A^CGT
MfeI 660 CAATTG C^AATTG
MluI 14 ACGCGT A^CGCGT
MroI 116 TCCGGA T^CCGGA
MslI 1318 CATCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 660 CAATTG C^AATTG
NspI 1294 GCATGC [GA]CATG^[CT]
PflFI 32 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 155 ACCGGT A^CCGGT
PpuMI 217 GGGACCT [GA]G^G[AT]CC[CT]
SexAI 220 ACCTGGT A^CC[AT]GGT
SphI 1294 GCATGC GCATG^C
Tth111I 32 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1027 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 15 CGCG CG^CG
108 CGCG
AcsI 282 AAATTC [GA]^AATT[CT]
1352 AAATTT
ApoI 282 AAATTC [GA]^AATT[CT]
1352 AAATTT
BalI 248 TGGCCA TGG^CCA
497 TGGCCA
BbsI 10 GAAGAC GAAGAC(2/6)
1019 GAAGAC
BpuAI 10 GAAGAC GAAGAC(2/6)
1019 GAAGAC
BsaWI 116 TCCGGA [AT]^CCGG[AT]
155 ACCGGT
BsmFI 202 GGGAC GGGAC(10/14)
217 GGGAC
BspMI 809 ACCTGC ACCTGC(4/8)
1218 ACCTGC
BstUI 15 CGCG CG^CG
108 CGCG
DpnII 207 GATC ^GATC
544 GATC
DraII 85 GGGGCCT [GA]G^G[ACGT]CC[CT]
217 GGGACCT
EaeI 248 TGGCCA [CT]^GGCC[GA]
497 TGGCCA
EcoO109I 85 GGGGCCT [GA]G^G[ACGT]CC[CT]
217 GGGACCT
HgaI 13 GACGC GACGC(5/10)
16 GCGTC
HincII 235 GTCAAC GT[CT]^[GA]AC
298 GTCAAC
HindII 235 GTCAAC GT[CT]^[GA]AC
298 GTCAAC
MaeIII 271 GTGAC ^GT[ACGT]AC
1180 GTGAC
MboI 207 GATC ^GATC
544 GATC
MluNI 248 TGGCCA TGG^CCA
497 TGGCCA
MscI 248 TGGCCA TGG^CCA
497 TGGCCA
MvnI 15 CGCG CG^CG
108 CGCG
NdeII 207 GATC ^GATC
544 GATC
PstI 885 CTGCAG CTGCA^G
1220 CTGCAG
PvuII 293 CAGCTG CAG^CTG
594 CAGCTG
Sau3AI 207 GATC ^GATC
544 GATC
Tsp45I 271 GTGAC ^GT[GC]AC
1180 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AfaI AluI BbvI BcnI
BsaJI BsiYI BslI BsmAI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII ItaI MboII
MnlI MseI MspA1I MspI MvaI
MwoI NciI NlaIII NlaIV PleI
RsaI Sau96I ScrFI SfcI TfiI
Tru9I TseI Tsp509I TspRI