################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AccI AclI AcyI AflII AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BamHI BanII BbeI BbrPI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BseRI BsgI BsiHKAI BsiWI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I CelII CfoI ClaI CpoI DraI DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HhaI Hin1I HinP1I HpaI KasI KpnI Ksp632I KspI MamI MflI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI TaqI TthHB8I Van91I XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccIII 116 TCCGGA T^CCGGA AflIII 14 ACGCGT A^C[GA][CT]GT AgeI 155 ACCGGT A^CCGGT AlwI 543 GGATC GGATC(4/5) AocI 818 CCTGAGG CC^T[ACGT]AGG AspI 32 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 218 GGACC G^G[AT]CC BanI 1156 GGTGCC G^G[CT][GA]CC BbvCI 1067 GCTGAGG CCTCAGC(-5/-2) BfaI 1028 CTAG C^TAG BmyI 1157 GTGCCC G[AGT]GC[ACT]^C BpmI 591 CTCCAG CTGGAG(16/14) BsaI 1088 GGTCTC GGTCTC(1/5) BseAI 116 TCCGGA T^CCGGA BsiEI 153 CGACCG CG[GA][CT]^CG BsmBI 375 CGTCTC CGTCTC(1/5) Bsp1286I 1157 GTGCCC G[AGT]GC[ACT]^C BspEI 116 TCCGGA T^CCGGA BsrFI 155 ACCGGT [GA]^CCGG[CT] BstAPI 167 GCACCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 818 CCTGAGG CC^T[ACGT]AGG Cfr10I 155 ACCGGT [GA]^CCGG[CT] DraIII 168 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 263 CCGTGG C^C[GA][CT]GG Eco81I 818 CCTGAGG CC^T[ACGT]AGG EcoNI 814 CCTGCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HindIII 73 AAGCTT A^AGCTT HphI 270 GGTGA GGTGA(8/7) MaeI 1028 CTAG C^TAG MaeII 1311 ACGT A^CGT MfeI 660 CAATTG C^AATTG MluI 14 ACGCGT A^CGCGT MroI 116 TCCGGA T^CCGGA MslI 1318 CATCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 660 CAATTG C^AATTG NspI 1294 GCATGC [GA]CATG^[CT] PflFI 32 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 155 ACCGGT A^CCGGT PpuMI 217 GGGACCT [GA]G^G[AT]CC[CT] SexAI 220 ACCTGGT A^CC[AT]GGT SphI 1294 GCATGC GCATG^C Tth111I 32 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 1027 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccII 15 CGCG CG^CG 108 CGCG AcsI 282 AAATTC [GA]^AATT[CT] 1352 AAATTT ApoI 282 AAATTC [GA]^AATT[CT] 1352 AAATTT BalI 248 TGGCCA TGG^CCA 497 TGGCCA BbsI 10 GAAGAC GAAGAC(2/6) 1019 GAAGAC BpuAI 10 GAAGAC GAAGAC(2/6) 1019 GAAGAC BsaWI 116 TCCGGA [AT]^CCGG[AT] 155 ACCGGT BsmFI 202 GGGAC GGGAC(10/14) 217 GGGAC BspMI 809 ACCTGC ACCTGC(4/8) 1218 ACCTGC BstUI 15 CGCG CG^CG 108 CGCG DpnII 207 GATC ^GATC 544 GATC DraII 85 GGGGCCT [GA]G^G[ACGT]CC[CT] 217 GGGACCT EaeI 248 TGGCCA [CT]^GGCC[GA] 497 TGGCCA EcoO109I 85 GGGGCCT [GA]G^G[ACGT]CC[CT] 217 GGGACCT HgaI 13 GACGC GACGC(5/10) 16 GCGTC HincII 235 GTCAAC GT[CT]^[GA]AC 298 GTCAAC HindII 235 GTCAAC GT[CT]^[GA]AC 298 GTCAAC MaeIII 271 GTGAC ^GT[ACGT]AC 1180 GTGAC MboI 207 GATC ^GATC 544 GATC MluNI 248 TGGCCA TGG^CCA 497 TGGCCA MscI 248 TGGCCA TGG^CCA 497 TGGCCA MvnI 15 CGCG CG^CG 108 CGCG NdeII 207 GATC ^GATC 544 GATC PstI 885 CTGCAG CTGCA^G 1220 CTGCAG PvuII 293 CAGCTG CAG^CTG 594 CAGCTG Sau3AI 207 GATC ^GATC 544 GATC Tsp45I 271 GTGAC ^GT[GC]AC 1180 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AciI AfaI AluI BbvI BcnI BsaJI BsiYI BslI BsmAI BssKI BstF5I BstNI Cac8I Cfr13I DdeI EcoRII FauI Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MboII MnlI MseI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV PleI RsaI Sau96I ScrFI SfcI TfiI Tru9I TseI Tsp509I TspRI