################################## # NO CUT ENZYMES ( 111 enzymes) # ################################## Acc65I AccI AclI AflII AgeI AhdI AlwNI Aor51HI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BbeI BciVI BclI BfaI BfrI BlnI BlpI Bpu1102I BsaBI BsaI BsiEI BsiWI BsmI BspDI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoO65I EcoRI FauI FbaI FseI FspI HpaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MspA1I MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AcsI 788 AAATTT [GA]^AATT[CT] Alw44I 1246 GTGCAC G^TGCAC AocI 1028 CCTCAGG CC^T[ACGT]AGG ApaI 1008 GGGCCC GGGCC^C ApaLI 1246 GTGCAC G^TGCAC ApoI 788 AAATTT [GA]^AATT[CT] Asp700I 958 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 139 GGACC G^G[AT]CC BalI 1125 TGGCCA TGG^CCA BbrPI 470 CACGTG CAC^GTG BbvCI 1330 CCTCAGC CCTCAGC(-5/-2) BcgI 426 GCACCAACGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 11 GCCACGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 597 AGATCT A^GATCT BpmI 674 CTGGAG CTGGAG(16/14) BsaAI 470 CACGTG [CT]AC^GT[GA] BsmBI 83 GAGACG CGTCTC(1/5) BspLU11I 1358 ACATGT A^CATGT BsrDI 232 CATTGC GCAATG(2/0) BsrI 870 ACTGG ACTGG(1/-1) BstXI 1209 CCATGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1028 CCTCAGG CC^T[ACGT]AGG DraIII 470 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 123 CCATGG C^C[GA][CT]GG Eco81I 1028 CCTCAGG CC^T[ACGT]AGG EcoNI 130 CCTGCGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 299 GATATC GAT^ATC EcoT14I 123 CCATGG C^C[AT][AT]GG EcoT22I 917 ATGCAT ATGCA^T HaeII 650 AGCGCC [GA]GCGC^[CT] MaeIII 104 GTGAC ^GT[ACGT]AC MluI 493 ACGCGT A^CGCGT MluNI 1125 TGGCCA TGG^CCA MscI 1125 TGGCCA TGG^CCA NcoI 123 CCATGG C^CATGG NsiI 917 ATGCAT ATGCA^T PmaCI 470 CACGTG CAC^GTG PmlI 470 CACGTG CAC^GTG PpuMI 138 AGGACCT [GA]G^G[AT]CC[CT] PstI 287 CTGCAG CTGCA^G ScaI 1179 AGTACT AGT^ACT SfcI 287 CTGCAG C^T[GA][CT]AG SmaI 1011 CCCGGG CCC^GGG StyI 123 CCATGG C^C[AT][AT]GG Tsp45I 104 GTGAC ^GT[GC]AC XmaCI 1011 CCCGGG C^CCGGG XmaI 1011 CCCGGG C^CCGGG XmnI 958 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AatII 22 GACGTC GACGT^C 1001 GACGTC AccIII 447 TCCGGA T^CCGGA 1155 TCCGGA AcyI 22 GACGTC G[GA]^CG[CT]C 1001 GACGTC AflIII 493 ACGCGT A^C[GA][CT]GT 1358 ACATGT AspHI 986 GTGCTC G[AT]GC[AT]^C 1246 GTGCAC AvaI 1011 CCCGGG C^[CT]CG[GA]G 1319 CTCGGG BanI 425 GGCACC G^G[CT][GA]CC 974 GGTGCC BanII 1008 GGGCCC G[GA]GC[CT]^C 1313 GAGCCC BbsI 1324 GAAGAC GAAGAC(2/6) 1304 GTCTTC BpuAI 1324 GAAGAC GAAGAC(2/6) 1304 GTCTTC BsaHI 22 GACGTC G[GA]^CG[CT]C 1001 GACGTC BseAI 447 TCCGGA T^CCGGA 1155 TCCGGA BseRI 56 GAGGAG GAGGAG(10/8) 92 GAGGAG BsgI 356 GTGCAG GTGCAG(16/14) 401 GTGCAG BsiHKAI 986 GTGCTC G[AT]GC[AT]^C 1246 GTGCAC BsoBI 1011 CCCGGG C^[CT]CG[GA]G 1319 CTCGGG BspEI 447 TCCGGA T^CCGGA 1155 TCCGGA BspMI 141 ACCTGC ACCTGC(4/8) 1056 ACCTGC BstYI 597 AGATCT [GA]^GATC[CT] 669 AGATCC EaeI 264 CGGCCA [CT]^GGCC[GA] 1125 TGGCCA HgaI 492 GACGC GACGC(5/10) 495 GCGTC Hin1I 22 GACGTC G[GA]^CG[CT]C 1001 GACGTC HincII 733 GTTGAC GT[CT]^[GA]AC 866 GTTGAC HindII 733 GTTGAC GT[CT]^[GA]AC 866 GTTGAC HindIII 321 AAGCTT A^AGCTT 1273 AAGCTT HphI 718 GGTGA GGTGA(8/7) 828 TCACC MflI 597 AGATCT [GA]^GATC[CT] 669 AGATCC MroI 447 TCCGGA T^CCGGA 1155 TCCGGA NspI 927 ACATGC [GA]CATG^[CT] 1358 ACATGT PleI 736 GACTC GAGTC(4/5) 947 GAGTC XcmI 409 CCATGCCTGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 705 CCATCCGCATCTTGG XhoII 597 AGATCT [GA]^GATC[CT] 669 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AccII AciI AfaI AluI AlwI BbvI BcnI BmyI BsaJI BsaWI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspHI BsrGI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeII MboI MboII MnlI MseI MslI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SspBI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I