##################################
# NO CUT ENZYMES ( 111 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
AhdI AlwNI Aor51HI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BbeI BciVI BclI
BfaI BfrI BlnI BlpI Bpu1102I
BsaBI BsaI BsiEI BsiWI BsmI
BspDI BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoO65I EcoRI
FauI FbaI FseI FspI HpaI
KasI KpnI Ksp632I KspI MaeI
MamI MfeI MspA1I MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tth111I Van91I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AcsI 788 AAATTT [GA]^AATT[CT]
Alw44I 1246 GTGCAC G^TGCAC
AocI 1028 CCTCAGG CC^T[ACGT]AGG
ApaI 1008 GGGCCC GGGCC^C
ApaLI 1246 GTGCAC G^TGCAC
ApoI 788 AAATTT [GA]^AATT[CT]
Asp700I 958 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 139 GGACC G^G[AT]CC
BalI 1125 TGGCCA TGG^CCA
BbrPI 470 CACGTG CAC^GTG
BbvCI 1330 CCTCAGC CCTCAGC(-5/-2)
BcgI 426 GCACCAACGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 11 GCCACGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 597 AGATCT A^GATCT
BpmI 674 CTGGAG CTGGAG(16/14)
BsaAI 470 CACGTG [CT]AC^GT[GA]
BsmBI 83 GAGACG CGTCTC(1/5)
BspLU11I 1358 ACATGT A^CATGT
BsrDI 232 CATTGC GCAATG(2/0)
BsrI 870 ACTGG ACTGG(1/-1)
BstXI 1209 CCATGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1028 CCTCAGG CC^T[ACGT]AGG
DraIII 470 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 123 CCATGG C^C[GA][CT]GG
Eco81I 1028 CCTCAGG CC^T[ACGT]AGG
EcoNI 130 CCTGCGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 299 GATATC GAT^ATC
EcoT14I 123 CCATGG C^C[AT][AT]GG
EcoT22I 917 ATGCAT ATGCA^T
HaeII 650 AGCGCC [GA]GCGC^[CT]
MaeIII 104 GTGAC ^GT[ACGT]AC
MluI 493 ACGCGT A^CGCGT
MluNI 1125 TGGCCA TGG^CCA
MscI 1125 TGGCCA TGG^CCA
NcoI 123 CCATGG C^CATGG
NsiI 917 ATGCAT ATGCA^T
PmaCI 470 CACGTG CAC^GTG
PmlI 470 CACGTG CAC^GTG
PpuMI 138 AGGACCT [GA]G^G[AT]CC[CT]
PstI 287 CTGCAG CTGCA^G
ScaI 1179 AGTACT AGT^ACT
SfcI 287 CTGCAG C^T[GA][CT]AG
SmaI 1011 CCCGGG CCC^GGG
StyI 123 CCATGG C^C[AT][AT]GG
Tsp45I 104 GTGAC ^GT[GC]AC
XmaCI 1011 CCCGGG C^CCGGG
XmaI 1011 CCCGGG C^CCGGG
XmnI 958 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AatII 22 GACGTC GACGT^C
1001 GACGTC
AccIII 447 TCCGGA T^CCGGA
1155 TCCGGA
AcyI 22 GACGTC G[GA]^CG[CT]C
1001 GACGTC
AflIII 493 ACGCGT A^C[GA][CT]GT
1358 ACATGT
AspHI 986 GTGCTC G[AT]GC[AT]^C
1246 GTGCAC
AvaI 1011 CCCGGG C^[CT]CG[GA]G
1319 CTCGGG
BanI 425 GGCACC G^G[CT][GA]CC
974 GGTGCC
BanII 1008 GGGCCC G[GA]GC[CT]^C
1313 GAGCCC
BbsI 1324 GAAGAC GAAGAC(2/6)
1304 GTCTTC
BpuAI 1324 GAAGAC GAAGAC(2/6)
1304 GTCTTC
BsaHI 22 GACGTC G[GA]^CG[CT]C
1001 GACGTC
BseAI 447 TCCGGA T^CCGGA
1155 TCCGGA
BseRI 56 GAGGAG GAGGAG(10/8)
92 GAGGAG
BsgI 356 GTGCAG GTGCAG(16/14)
401 GTGCAG
BsiHKAI 986 GTGCTC G[AT]GC[AT]^C
1246 GTGCAC
BsoBI 1011 CCCGGG C^[CT]CG[GA]G
1319 CTCGGG
BspEI 447 TCCGGA T^CCGGA
1155 TCCGGA
BspMI 141 ACCTGC ACCTGC(4/8)
1056 ACCTGC
BstYI 597 AGATCT [GA]^GATC[CT]
669 AGATCC
EaeI 264 CGGCCA [CT]^GGCC[GA]
1125 TGGCCA
HgaI 492 GACGC GACGC(5/10)
495 GCGTC
Hin1I 22 GACGTC G[GA]^CG[CT]C
1001 GACGTC
HincII 733 GTTGAC GT[CT]^[GA]AC
866 GTTGAC
HindII 733 GTTGAC GT[CT]^[GA]AC
866 GTTGAC
HindIII 321 AAGCTT A^AGCTT
1273 AAGCTT
HphI 718 GGTGA GGTGA(8/7)
828 TCACC
MflI 597 AGATCT [GA]^GATC[CT]
669 AGATCC
MroI 447 TCCGGA T^CCGGA
1155 TCCGGA
NspI 927 ACATGC [GA]CATG^[CT]
1358 ACATGT
PleI 736 GACTC GAGTC(4/5)
947 GAGTC
XcmI 409 CCATGCCTGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
705 CCATCCGCATCTTGG
XhoII 597 AGATCT [GA]^GATC[CT]
669 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
BbvI BcnI BmyI BsaJI BsaWI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspHI BsrGI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MaeII
MboI MboII MnlI MseI MslI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SspBI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I