##################################
# NO CUT ENZYMES ( 180 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaII AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaJI
BsaWI BseAI BsgI BsiEI BsiWI
BsmI Bsp1407I BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI BstZ17I CelII CfoI Cfr10I
Cfr13I CpoI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRI
EcoRII EcoRV EcoT14I EcoT22I FauI
FbaI FokI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MflI
MluI MluNI MroI MscI MslI
MunI MvaI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TfiI Tth111I Van91I XbaI
XcmI XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccI 356 GTCTAC GT^[AC][GT]AC
AlwI 95 GGATC GGATC(4/5)
AocI 80 CCTCAGG CC^T[ACGT]AGG
AspHI 134 GAGCTC G[AT]GC[AT]^C
AvaI 137 CTCGAG C^[CT]CG[GA]G
BcnI 29 CCCGG CC^[GC]GG
BglI 176 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiHKAI 134 GAGCTC G[AT]GC[AT]^C
BsiYI 706 CCCCTTTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 706 CCCCTTTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 542 GAGACG CGTCTC(1/5)
BsmFI 50 GTCCC GGGAC(10/14)
BsoBI 137 CTCGAG C^[CT]CG[GA]G
BspDI 200 ATCGAT AT^CGAT
BssKI 29 CCCGG ^CC[ACGT]GG
Bsu36I 80 CCTCAGG CC^T[ACGT]AGG
ClaI 200 ATCGAT AT^CGAT
DraI 744 TTTAAA TTT^AAA
EaeI 162 CGGCCA [CT]^GGCC[GA]
EarI 527 GAAGAG CTCTTC(1/4)
Eco81I 80 CCTCAGG CC^T[ACGT]AGG
HaeIII 163 GGCC GG^CC
HapII 30 CCGG C^CGG
HpaII 30 CCGG C^CGG
Ksp632I 527 GAAGAG CTCTTC(1/4)
MaeII 591 ACGT A^CGT
MspI 30 CCGG C^CGG
NciI 29 CCCGG CC^[GC]GG
NlaIII 577 CATG CATG^
NlaIV 4 GGTTCC GG[ACGT]^[ACGT]CC
PaeR7I 137 CTCGAG C^TCGAG
SacI 134 GAGCTC GAGCT^C
ScrFI 29 CCCGG CC^[ACGT]GG
SmlI 137 CTCGAG C^T[CT][GA]AG
Tsp45I 305 GTCAC ^GT[GC]AC
TspRI 115 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoI 137 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AciI 17 CCGC CCGC(-3/-1)
189 CCGC
AcsI 449 AAATTC [GA]^AATT[CT]
748 AAATTT
AfaI 128 GTAC GT^AC
754 GTAC
AlwNI 105 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
438 CAGCTGCTG
ApoI 449 AAATTC [GA]^AATT[CT]
748 AAATTT
BbsI 686 GAAGAC GAAGAC(2/6)
276 GTCTTC
BpuAI 686 GAAGAC GAAGAC(2/6)
276 GTCTTC
BseRI 515 GAGGAG GAGGAG(10/8)
530 GAGGAG
BsrI 271 ACTGG ACTGG(1/-1)
571 ACTGG
BstAPI 19 GCACAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
64 GCAGGAGTTGC
HphI 32 GGTGA GGTGA(8/7)
464 GGTGA
MspA1I 179 CAGCTG C[AC]G^C[GT]G
438 CAGCTG
PvuII 179 CAGCTG CAG^CTG
438 CAGCTG
RsaI 128 GTAC GT^AC
754 GTAC
SfcI 57 CTACAG C^T[GA][CT]AG
733 CTACAG
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AluI BanII BbvI BmyI BsmAI
Bsp1286I Cac8I DdeI DpnII Fnu4HI
HinfI ItaI MaeIII MboI MboII
MnlI MseI MwoI NdeII PleI
Sau3AI TaqI Tru9I TseI Tsp509I
TthHB8I