##################################
# NO CUT ENZYMES ( 163 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbsI BbvCI BcgI
BciVI BclI BcnI BglI BglII
BlnI BpuAI BsaBI BsaHI BsaI
BseAI BsiEI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BsrBI BsrDI BsrGI BssHII
BssKI BssSI Bst1107I BstBI BstEII
BstNI BstPI BstUI BstZ17I Bsu36I
CfoI ClaI CpoI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRII
EcoRV FauI FbaI FseI FspI
HaeII HaeIII HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
Ksp632I KspI MamI MfeI MluI
MluNI MroI MscI MunI MvaI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflMI PleI
PmeI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
ScrFI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
TaqI TthHB8I Van91I XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AciI 366 GCGG CCGC(-3/-1)
AflII 772 CTTAAG C^TTAAG
AflIII 230 ACATGT A^C[GA][CT]GT
AgeI 161 ACCGGT A^CCGGT
AlwI 84 GGATC GGATC(4/5)
AlwNI 524 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 506 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 493 CACGTG CAC^GTG
BfrI 772 CTTAAG C^TTAAG
BlpI 541 GCTAAGC GC^T[ACGT]AGC
BpmI 13 CTGGAG CTGGAG(16/14)
Bpu1102I 541 GCTAAGC GC^T[ACGT]AGC
BsaAI 493 CACGTG [CT]AC^GT[GA]
BsaWI 161 ACCGGT [AT]^CCGG[AT]
BsgI 138 CTGCAC GTGCAG(16/14)
BsiYI 582 CCCCTCCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 582 CCCCTCCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 273 GTCTC GTCTC(1/5)
BspLU11I 230 ACATGT A^CATGT
BspMI 658 ACCTGC ACCTGC(4/8)
BsrFI 161 ACCGGT [GA]^CCGG[CT]
BstXI 640 CCAGCTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 84 GGATCT [GA]^GATC[CT]
Cac8I 765 GCCAGC GC[ACGT]^[ACGT]GC
CelII 541 GCTAAGC GC^T[ACGT]AGC
Cfr10I 161 ACCGGT [GA]^CCGG[CT]
DpnII 85 GATC ^GATC
EcoT14I 587 CCTTGG C^C[AT][AT]GG
EcoT22I 609 ATGCAT ATGCA^T
HapII 162 CCGG C^CGG
HgaI 134 GACGC GACGC(5/10)
HindIII 26 AAGCTT A^AGCTT
HpaII 162 CCGG C^CGG
HphI 519 GGTGA GGTGA(8/7)
MboI 85 GATC ^GATC
MboII 241 GAAGA GAAGA(8/7)
MflI 84 GGATCT [GA]^GATC[CT]
MslI 182 CACTCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 162 CCGG C^CGG
NdeII 85 GATC ^GATC
NlaIV 636 GGGTCC GG[ACGT]^[ACGT]CC
NsiI 609 ATGCAT ATGCA^T
PflFI 506 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 161 ACCGGT A^CCGGT
PmaCI 493 CACGTG CAC^GTG
PmlI 493 CACGTG CAC^GTG
Sau3AI 85 GATC ^GATC
SfcI 143 CTATAG C^T[GA][CT]AG
SspI 821 AATATT AAT^ATT
StyI 587 CCTTGG C^C[AT][AT]GG
Tth111I 506 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 486 TCTAGA T^CTAGA
XhoII 84 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 103 AAATTT [GA]^AATT[CT]
388 GAATTT
AfaI 697 GTAC GT^AC
827 GTAC
ApoI 103 AAATTT [GA]^AATT[CT]
388 GAATTT
AvaII 575 GGACC G^G[AT]CC
637 GGTCC
BbvI 526 GCAGC GCAGC(8/12)
137 GCTGC
BsaJI 448 CCTCGG C^C[ACGT][ACGT]GG
587 CCTTGG
BseRI 16 GAGGAG GAGGAG(10/8)
302 GAGGAG
BstAPI 438 GCACTTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
756 GCATAATCTGC
BstF5I 689 CATCC GGATG(2/0)
532 GGATG
Cfr13I 575 GGACC G^G[ACGT]CC
637 GGTCC
DraI 372 TTTAAA TTT^AAA
594 TTTAAA
Fnu4HI 137 GCTGC GC^[ACGT]GC
526 GCAGC
FokI 689 CATCC GGATG(9/13)
532 GGATG
HinfI 224 GATTC G^A[ACGT]TC
863 GAATC
ItaI 137 GCTGC GC^[ACGT]GC
526 GCAGC
MaeII 494 ACGT A^CGT
564 ACGT
MspA1I 71 CAGCTG C[AC]G^C[GT]G
527 CAGCTG
NspI 230 ACATGT [GA]CATG^[CT]
607 ACATGC
PvuII 71 CAGCTG CAG^CTG
527 CAGCTG
RsaI 697 GTAC GT^AC
827 GTAC
Sau96I 575 GGACC G^G[ACGT]CC
637 GGTCC
SmlI 701 CTCAAG C^T[CT][GA]AG
772 CTTAAG
TfiI 224 GATTC G^A[AT]TC
863 GAATC
TseI 137 GCTGC G^C[AT]GC
526 GCAGC
Tsp45I 294 GTCAC ^GT[GC]AC
520 GTGAC
TspRI 296 CACTG CA[GC]TG[ACGT][ACGT]^
571 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AluI AspHI BfaI BmyI BsiHKAI
Bsp1286I BsrI DdeI MaeI MaeIII
MnlI MseI MwoI NlaIII Tru9I
Tsp509I