################################## # NO CUT ENZYMES ( 163 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BcnI BglI BglII BlnI BpuAI BsaBI BsaHI BsaI BseAI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssKI BssSI Bst1107I BstBI BstEII BstNI BstPI BstUI BstZ17I Bsu36I CfoI ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV FauI FbaI FseI FspI HaeII HaeIII HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MunI MvaI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflMI PleI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI ScrFI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI TthHB8I Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AciI 366 GCGG CCGC(-3/-1) AflII 772 CTTAAG C^TTAAG AflIII 230 ACATGT A^C[GA][CT]GT AgeI 161 ACCGGT A^CCGGT AlwI 84 GGATC GGATC(4/5) AlwNI 524 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG AspI 506 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 493 CACGTG CAC^GTG BfrI 772 CTTAAG C^TTAAG BlpI 541 GCTAAGC GC^T[ACGT]AGC BpmI 13 CTGGAG CTGGAG(16/14) Bpu1102I 541 GCTAAGC GC^T[ACGT]AGC BsaAI 493 CACGTG [CT]AC^GT[GA] BsaWI 161 ACCGGT [AT]^CCGG[AT] BsgI 138 CTGCAC GTGCAG(16/14) BsiYI 582 CCCCTCCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 582 CCCCTCCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 273 GTCTC GTCTC(1/5) BspLU11I 230 ACATGT A^CATGT BspMI 658 ACCTGC ACCTGC(4/8) BsrFI 161 ACCGGT [GA]^CCGG[CT] BstXI 640 CCAGCTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 84 GGATCT [GA]^GATC[CT] Cac8I 765 GCCAGC GC[ACGT]^[ACGT]GC CelII 541 GCTAAGC GC^T[ACGT]AGC Cfr10I 161 ACCGGT [GA]^CCGG[CT] DpnII 85 GATC ^GATC EcoT14I 587 CCTTGG C^C[AT][AT]GG EcoT22I 609 ATGCAT ATGCA^T HapII 162 CCGG C^CGG HgaI 134 GACGC GACGC(5/10) HindIII 26 AAGCTT A^AGCTT HpaII 162 CCGG C^CGG HphI 519 GGTGA GGTGA(8/7) MboI 85 GATC ^GATC MboII 241 GAAGA GAAGA(8/7) MflI 84 GGATCT [GA]^GATC[CT] MslI 182 CACTCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 162 CCGG C^CGG NdeII 85 GATC ^GATC NlaIV 636 GGGTCC GG[ACGT]^[ACGT]CC NsiI 609 ATGCAT ATGCA^T PflFI 506 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 161 ACCGGT A^CCGGT PmaCI 493 CACGTG CAC^GTG PmlI 493 CACGTG CAC^GTG Sau3AI 85 GATC ^GATC SfcI 143 CTATAG C^T[GA][CT]AG SspI 821 AATATT AAT^ATT StyI 587 CCTTGG C^C[AT][AT]GG Tth111I 506 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 486 TCTAGA T^CTAGA XhoII 84 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcsI 103 AAATTT [GA]^AATT[CT] 388 GAATTT AfaI 697 GTAC GT^AC 827 GTAC ApoI 103 AAATTT [GA]^AATT[CT] 388 GAATTT AvaII 575 GGACC G^G[AT]CC 637 GGTCC BbvI 526 GCAGC GCAGC(8/12) 137 GCTGC BsaJI 448 CCTCGG C^C[ACGT][ACGT]GG 587 CCTTGG BseRI 16 GAGGAG GAGGAG(10/8) 302 GAGGAG BstAPI 438 GCACTTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 756 GCATAATCTGC BstF5I 689 CATCC GGATG(2/0) 532 GGATG Cfr13I 575 GGACC G^G[ACGT]CC 637 GGTCC DraI 372 TTTAAA TTT^AAA 594 TTTAAA Fnu4HI 137 GCTGC GC^[ACGT]GC 526 GCAGC FokI 689 CATCC GGATG(9/13) 532 GGATG HinfI 224 GATTC G^A[ACGT]TC 863 GAATC ItaI 137 GCTGC GC^[ACGT]GC 526 GCAGC MaeII 494 ACGT A^CGT 564 ACGT MspA1I 71 CAGCTG C[AC]G^C[GT]G 527 CAGCTG NspI 230 ACATGT [GA]CATG^[CT] 607 ACATGC PvuII 71 CAGCTG CAG^CTG 527 CAGCTG RsaI 697 GTAC GT^AC 827 GTAC Sau96I 575 GGACC G^G[ACGT]CC 637 GGTCC SmlI 701 CTCAAG C^T[CT][GA]AG 772 CTTAAG TfiI 224 GATTC G^A[AT]TC 863 GAATC TseI 137 GCTGC G^C[AT]GC 526 GCAGC Tsp45I 294 GTCAC ^GT[GC]AC 520 GTGAC TspRI 296 CACTG CA[GC]TG[ACGT][ACGT]^ 571 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 16 enzymes) # ######################################### AluI AspHI BfaI BmyI BsiHKAI Bsp1286I BsrI DdeI MaeI MaeIII MnlI MseI MwoI NlaIII Tru9I Tsp509I