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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflIII AgeI AhdI AlwI
AlwNI Aor51HI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanI BbeI BbsI
BbvCI BciVI BcnI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaHI BsaI BseRI BsiEI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRV EcoT14I EcoT22I FseI FspI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MamI MfeI MflI MluI
MluNI MscI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIII NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StyI SwaI Tth111I Van91I
XbaI XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccIII 253 TCCGGA T^CCGGA
AcsI 218 GAATTC [GA]^AATT[CT]
AflII 785 CTTAAG C^TTAAG
AocI 358 CCTGAGG CC^T[ACGT]AGG
ApaI 411 GGGCCC GGGCC^C
ApoI 218 GAATTC [GA]^AATT[CT]
AvaI 491 CTCGGG C^[CT]CG[GA]G
BanII 411 GGGCCC G[GA]GC[CT]^C
BbrPI 709 CACGTG CAC^GTG
BcgI 131 CGACAGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 116 TGATCA T^GATCA
BfrI 785 CTTAAG C^TTAAG
BsaAI 709 CACGTG [CT]AC^GT[GA]
BsaJI 655 CCCTGG C^C[ACGT][ACGT]GG
BsaWI 253 TCCGGA [AT]^CCGG[AT]
BseAI 253 TCCGGA T^CCGGA
BsmBI 698 CGTCTC CGTCTC(1/5)
BsmFI 448 GTCCC GGGAC(10/14)
BsoBI 491 CTCGGG C^[CT]CG[GA]G
BspEI 253 TCCGGA T^CCGGA
BspMI 284 ACCTGC ACCTGC(4/8)
BsrDI 460 CATTGC GCAATG(2/0)
BsrI 292 CCAGT ACTGG(1/-1)
BstF5I 260 GGATG GGATG(2/0)
BstXI 292 CCAGTGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 358 CCTGAGG CC^T[ACGT]AGG
DpnII 117 GATC ^GATC
Eco81I 358 CCTGAGG CC^T[ACGT]AGG
EcoRI 218 GAATTC G^AATTC
FbaI 116 TGATCA T^GATCA
FokI 260 GGATG GGATG(9/13)
HapII 254 CCGG C^CGG
HpaII 254 CCGG C^CGG
MaeII 710 ACGT A^CGT
MboI 117 GATC ^GATC
MroI 253 TCCGGA T^CCGGA
MseI 786 TTAA T^TAA
MslI 534 CACAGGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 254 CCGG C^CGG
NdeII 117 GATC ^GATC
PmaCI 709 CACGTG CAC^GTG
PmlI 709 CACGTG CAC^GTG
PpuMI 624 AGGACCT [GA]G^G[AT]CC[CT]
PstI 351 CTGCAG CTGCA^G
Sau3AI 117 GATC ^GATC
ScaI 789 AGTACT AGT^ACT
SfcI 351 CTGCAG C^T[GA][CT]AG
StuI 355 AGGCCT AGG^CCT
TaqI 130 TCGA T^CGA
Tru9I 786 TTAA T^TAA
TthHB8I 130 TCGA T^CGA
XcmI 518 CCACAGGCTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Alw44I 139 GTGCAC G^TGCAC
613 GTGCAC
ApaLI 139 GTGCAC G^TGCAC
613 GTGCAC
Asp700I 214 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
797 GAAAACATTC
AvaII 363 GGTCC G^G[AT]CC
625 GGACC
BbvI 487 GCTGC GCAGC(8/12)
641 GCTGC
BfaI 239 CTAG C^TAG
557 CTAG
BpmI 290 CTCCAG CTGGAG(16/14)
300 CTGGAG
BsgI 642 CTGCAC GTGCAG(16/14)
752 GTGCAG
BsiYI 458 CCCATTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
498 CCTCCCTGTGG
BslI 458 CCCATTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
498 CCTCCCTGTGG
CfoI 320 GCGC GCG^C
639 GCGC
HhaI 320 GCGC GCG^C
639 GCGC
HinP1I 320 GCGC G^CGC
639 GCGC
MaeI 239 CTAG C^TAG
557 CTAG
MboII 43 GAAGA GAAGA(8/7)
247 GAAGA
MspA1I 134 CAGCTG C[AC]G^C[GT]G
416 CAGCTG
PleI 514 GACTC GAGTC(4/5)
766 GACTC
PvuII 134 CAGCTG CAG^CTG
416 CAGCTG
SfaNI 276 GCATC GCATC(5/9)
721 GCATC
SmlI 572 CTTGAG C^T[CT][GA]AG
785 CTTAAG
TseI 487 GCTGC G^C[AT]GC
641 GCTGC
Tsp509I 219 AATT ^AATT
682 AATT
TspRI 1 CAGTG CA[GC]TG[ACGT][ACGT]^
293 CAGTG
XmnI 214 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
797 GAAAACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AfaI AluI AspHI BmyI
BsiHKAI BsmAI Bsp1286I BssKI BstNI
Cac8I Cfr13I DdeI DraII EcoO109I
EcoRII FauI Fnu4HI HaeIII HinfI
HphI ItaI MaeIII MnlI MvaI
MwoI NlaIV RsaI Sau96I ScrFI
TfiI Tsp45I