##################################
# NO CUT ENZYMES ( 133 enzymes) #
##################################
AccIII AclI AcsI AflII AgeI
AhdI Alw44I ApaI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AviII AvrII BalI BamHI
BbeI BbrPI BbsI BciVI BclI
BfrI BglI BlnI BpuAI BsaAI
BsaBI BsaWI BseAI BseRI BsgI
BsiEI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HinfI HpaI KasI MamI MfeI
MluI MluNI MroI MscI MseI
MslI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NlaIII NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
SexAI SfiI SfoI SfuI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tsp45I Tth111I Van91I XbaI XcmI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 276 GACGTC GACGT^C
Acc65I 741 GGTACC G^GTACC
AccI 827 GTCTAC GT^[AC][GT]AC
AflIII 647 ACGTGT A^C[GA][CT]GT
AocI 369 CCTAAGG CC^T[ACGT]AGG
Aor51HI 496 AGCGCT AGC^GCT
Asp718I 741 GGTACC G^GTACC
AspHI 196 GAGCTC G[AT]GC[AT]^C
BanI 741 GGTACC G^G[CT][GA]CC
BbvCI 525 GCTGAGG CCTCAGC(-5/-2)
BcgI 673 GCACTCAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 73 AGATCT A^GATCT
BlpI 430 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 430 GCTGAGC GC^T[ACGT]AGC
BsaI 81 GGTCTC GGTCTC(1/5)
BsiHKAI 196 GAGCTC G[AT]GC[AT]^C
BsiWI 306 CGTACG C^GTACG
BsrFI 230 GCCGGT [GA]^CCGG[CT]
BssHII 506 GCGCGC G^CGCGC
BstF5I 447 GGATG GGATG(2/0)
Bsu36I 369 CCTAAGG CC^T[ACGT]AGG
CelII 430 GCTGAGC GC^T[ACGT]AGC
Cfr10I 230 GCCGGT [GA]^CCGG[CT]
DraII 783 AGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 25 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 717 TGGCCG [CT]^GGCC[GA]
Eco47III 496 AGCGCT AGC^GCT
Eco81I 369 CCTAAGG CC^T[ACGT]AGG
EcoO109I 783 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 474 CCTTGG C^C[AT][AT]GG
FauI 691 GCGGG CCCGC(4/6)
FokI 447 GGATG GGATG(9/13)
HindIII 107 AAGCTT A^AGCTT
HphI 706 TCACC GGTGA(8/7)
KpnI 741 GGTACC GGTAC^C
KspI 402 CCGCGG CCGC^GG
MaeIII 360 GTTAC ^GT[ACGT]AC
PaeR7I 568 CTCGAG C^TCGAG
PpuMI 783 AGGTCCC [GA]G^G[AT]CC[CT]
PvuII 325 CAGCTG CAG^CTG
SacI 196 GAGCTC GAGCT^C
SacII 402 CCGCGG CCGC^GG
SapI 729 GCTCTTC GCTCTTC(1/4)
ScaI 183 AGTACT AGT^ACT
SgrAI 229 CGCCGGTG C[GA]^CCGG[CT]G
StyI 474 CCTTGG C^C[AT][AT]GG
TaqI 569 TCGA T^CGA
Tsp509I 593 AATT ^AATT
TthHB8I 569 TCGA T^CGA
XhoI 568 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwI 161 GGATC GGATC(4/5)
612 GGATC
AlwNI 202 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
325 CAGCTGCTG
AvaI 568 CTCGAG C^[CT]CG[GA]G
653 CCCGAG
BanII 196 GAGCTC G[GA]GC[CT]^C
374 GGGCTC
BcnI 113 CCGGG CC^[GC]GG
723 CCGGG
BfaI 256 CTAG C^TAG
394 CTAG
BpmI 125 CTCCAG CTGGAG(16/14)
199 CTCCAG
BsmFI 651 GTCCC GGGAC(10/14)
785 GTCCC
BsoBI 568 CTCGAG C^[CT]CG[GA]G
653 CCCGAG
BspMI 635 ACCTGC ACCTGC(4/8)
189 GCAGGT
BsrBI 215 CCGCTC CCGCTC(-3/-3)
415 GAGCGG
BsrI 715 ACTGG ACTGG(1/-1)
127 CCAGT
BstYI 73 AGATCT [GA]^GATC[CT]
161 GGATCT
EarI 730 CTCTTC CTCTTC(1/4)
490 GAAGAG
HaeII 496 AGCGCT [GA]GCGC^[CT]
726 GGCGCT
HgaI 10 GACGC GACGC(5/10)
747 GACGC
Ksp632I 730 CTCTTC CTCTTC(1/4)
490 GAAGAG
MaeI 256 CTAG C^TAG
394 CTAG
MflI 73 AGATCT [GA]^GATC[CT]
161 GGATCT
MspA1I 325 CAGCTG C[AC]G^C[GT]G
402 CCGCGG
NciI 113 CCGGG CC^[GC]GG
723 CCGGG
PstI 187 CTGCAG CTGCA^G
685 CTGCAG
SfcI 187 CTGCAG C^T[GA][CT]AG
685 CTGCAG
SmlI 568 CTCGAG C^T[CT][GA]AG
582 CTTGAG
XhoII 73 AGATCT [GA]^GATC[CT]
161 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaII BbvI BmyI BsaHI BsaJI
BsiYI BslI BsmAI BsmBI Bsp1286I
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EcoRII
Fnu4HI HaeIII HapII HhaI Hin1I
HinP1I HpaII ItaI MaeII MboI
MboII MnlI MspI MvaI MvnI
MwoI NdeII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI TseI TspRI