################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AccIII AclI AcsI AflII AgeI AhdI Alw44I ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BbsI BciVI BclI BfrI BglI BlnI BpuAI BsaAI BsaBI BsaWI BseAI BseRI BsgI BsiEI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HinfI HpaI KasI MamI MfeI MluI MluNI MroI MscI MseI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI SexAI SfiI SfoI SfuI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp45I Tth111I Van91I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AatII 276 GACGTC GACGT^C Acc65I 741 GGTACC G^GTACC AccI 827 GTCTAC GT^[AC][GT]AC AflIII 647 ACGTGT A^C[GA][CT]GT AocI 369 CCTAAGG CC^T[ACGT]AGG Aor51HI 496 AGCGCT AGC^GCT Asp718I 741 GGTACC G^GTACC AspHI 196 GAGCTC G[AT]GC[AT]^C BanI 741 GGTACC G^G[CT][GA]CC BbvCI 525 GCTGAGG CCTCAGC(-5/-2) BcgI 673 GCACTCAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 73 AGATCT A^GATCT BlpI 430 GCTGAGC GC^T[ACGT]AGC Bpu1102I 430 GCTGAGC GC^T[ACGT]AGC BsaI 81 GGTCTC GGTCTC(1/5) BsiHKAI 196 GAGCTC G[AT]GC[AT]^C BsiWI 306 CGTACG C^GTACG BsrFI 230 GCCGGT [GA]^CCGG[CT] BssHII 506 GCGCGC G^CGCGC BstF5I 447 GGATG GGATG(2/0) Bsu36I 369 CCTAAGG CC^T[ACGT]AGG CelII 430 GCTGAGC GC^T[ACGT]AGC Cfr10I 230 GCCGGT [GA]^CCGG[CT] DraII 783 AGGTCCC [GA]G^G[ACGT]CC[CT] DraIII 25 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 717 TGGCCG [CT]^GGCC[GA] Eco47III 496 AGCGCT AGC^GCT Eco81I 369 CCTAAGG CC^T[ACGT]AGG EcoO109I 783 AGGTCCC [GA]G^G[ACGT]CC[CT] EcoT14I 474 CCTTGG C^C[AT][AT]GG FauI 691 GCGGG CCCGC(4/6) FokI 447 GGATG GGATG(9/13) HindIII 107 AAGCTT A^AGCTT HphI 706 TCACC GGTGA(8/7) KpnI 741 GGTACC GGTAC^C KspI 402 CCGCGG CCGC^GG MaeIII 360 GTTAC ^GT[ACGT]AC PaeR7I 568 CTCGAG C^TCGAG PpuMI 783 AGGTCCC [GA]G^G[AT]CC[CT] PvuII 325 CAGCTG CAG^CTG SacI 196 GAGCTC GAGCT^C SacII 402 CCGCGG CCGC^GG SapI 729 GCTCTTC GCTCTTC(1/4) ScaI 183 AGTACT AGT^ACT SgrAI 229 CGCCGGTG C[GA]^CCGG[CT]G StyI 474 CCTTGG C^C[AT][AT]GG TaqI 569 TCGA T^CGA Tsp509I 593 AATT ^AATT TthHB8I 569 TCGA T^CGA XhoI 568 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AlwI 161 GGATC GGATC(4/5) 612 GGATC AlwNI 202 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG 325 CAGCTGCTG AvaI 568 CTCGAG C^[CT]CG[GA]G 653 CCCGAG BanII 196 GAGCTC G[GA]GC[CT]^C 374 GGGCTC BcnI 113 CCGGG CC^[GC]GG 723 CCGGG BfaI 256 CTAG C^TAG 394 CTAG BpmI 125 CTCCAG CTGGAG(16/14) 199 CTCCAG BsmFI 651 GTCCC GGGAC(10/14) 785 GTCCC BsoBI 568 CTCGAG C^[CT]CG[GA]G 653 CCCGAG BspMI 635 ACCTGC ACCTGC(4/8) 189 GCAGGT BsrBI 215 CCGCTC CCGCTC(-3/-3) 415 GAGCGG BsrI 715 ACTGG ACTGG(1/-1) 127 CCAGT BstYI 73 AGATCT [GA]^GATC[CT] 161 GGATCT EarI 730 CTCTTC CTCTTC(1/4) 490 GAAGAG HaeII 496 AGCGCT [GA]GCGC^[CT] 726 GGCGCT HgaI 10 GACGC GACGC(5/10) 747 GACGC Ksp632I 730 CTCTTC CTCTTC(1/4) 490 GAAGAG MaeI 256 CTAG C^TAG 394 CTAG MflI 73 AGATCT [GA]^GATC[CT] 161 GGATCT MspA1I 325 CAGCTG C[AC]G^C[GT]G 402 CCGCGG NciI 113 CCGGG CC^[GC]GG 723 CCGGG PstI 187 CTGCAG CTGCA^G 685 CTGCAG SfcI 187 CTGCAG C^T[GA][CT]AG 685 CTGCAG SmlI 568 CTCGAG C^T[CT][GA]AG 582 CTTGAG XhoII 73 AGATCT [GA]^GATC[CT] 161 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AvaII BbvI BmyI BsaHI BsaJI BsiYI BslI BsmAI BsmBI Bsp1286I BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DsaI EcoRII Fnu4HI HaeIII HapII HhaI Hin1I HinP1I HpaII ItaI MaeII MboI MboII MnlI MspI MvaI MvnI MwoI NdeII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TseI TspRI