################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## AatII Acc65I AccII AclI AcyI AgeI Alw44I AlwI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AviII BamHI BanI BbeI BbrPI BclI BglI BglII BlpI Bpu1102I BsaAI BsaBI BsaHI BsiEI BsiWI BsmI Bsp1407I BspDI BspHI BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstUI BstXI BstYI BstZ17I CelII Cfr10I ClaI DpnII DraI DraIII DrdI EagI EclXI Eco47III Eco52I EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MboI MflI MluI MvnI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI SacI SacII SalI SapI Sau3AI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TaqI TfiI TthHB8I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 64 enzymes) # ################################### AccI 495 GTCTAC GT^[AC][GT]AC AccIII 189 TCCGGA T^CCGGA AcsI 522 GAATTC [GA]^AATT[CT] AflII 920 CTTAAG C^TTAAG AflIII 172 ACATGT A^C[GA][CT]GT AhdI 32 GACGGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 581 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1091 CCTCAGG CC^T[ACGT]AGG ApoI 522 GAATTC [GA]^AATT[CT] AspEI 32 GACGGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 417 GTGCTC G[AT]GC[AT]^C AspI 477 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 630 CCCGGG C^[CT]CG[GA]G AvrII 382 CCTAGG C^CTAGG BanII 1148 GGGCTC G[GA]GC[CT]^C BbsI 345 GTCTTC GAAGAC(2/6) BbvCI 8 GCTGAGG CCTCAGC(-5/-2) BcgI 309 CGACTGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 756 GTATCC GTATCC(6/5) BfrI 920 CTTAAG C^TTAAG BlnI 382 CCTAGG C^CTAGG BpuAI 345 GTCTTC GAAGAC(2/6) BsaWI 189 TCCGGA [AT]^CCGG[AT] BseAI 189 TCCGGA T^CCGGA BseRI 260 CTCCTC GAGGAG(10/8) BsgI 510 CTGCAC GTGCAG(16/14) BsiHKAI 417 GTGCTC G[AT]GC[AT]^C BsmBI 818 GAGACG CGTCTC(1/5) BsoBI 630 CCCGGG C^[CT]CG[GA]G BspEI 189 TCCGGA T^CCGGA BspLU11I 172 ACATGT A^CATGT BspMI 164 GCAGGT ACCTGC(4/8) BssSI 828 CTCGTG CACGAG(-5/-1) BstEII 1096 GGTCACC G^GT[ACGT]ACC BstPI 1096 GGTCACC G^GT[ACGT]ACC Bsu36I 1091 CCTCAGG CC^T[ACGT]AGG CfoI 430 GCGC GCG^C CpoI 562 CGGACCG CG^G[AT]CCG DsaI 556 CCGTGG C^C[GA][CT]GG Eam1105I 32 GACGGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1091 CCTCAGG CC^T[ACGT]AGG EcoNI 1122 CCTGACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1096 GGTCACC G^GT[ACGT]ACC EcoRI 522 GAATTC G^AATTC EcoT14I 382 CCTAGG C^C[AT][AT]GG HaeII 429 AGCGCC [GA]GCGC^[CT] HhaI 430 GCGC GCG^C HinP1I 430 GCGC G^CGC HinfI 810 GACTC G^A[ACGT]TC MfeI 899 CAATTG C^AATTG MroI 189 TCCGGA T^CCGGA MslI 70 CATCATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 899 CAATTG C^AATTG PflFI 477 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 810 GACTC GAGTC(4/5) PstI 132 CTGCAG CTGCA^G PvuII 848 CAGCTG CAG^CTG RsrII 562 CGGACCG CG^G[AT]CCG SmaI 630 CCCGGG CCC^GGG SphI 668 GCATGC GCATG^C StyI 382 CCTAGG C^C[AT][AT]GG Tth111I 477 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 630 CCCGGG C^CCGGG XmaI 630 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### BalI 84 TGGCCA TGG^CCA 1273 TGGCCA BcnI 630 CCCGG CC^[GC]GG 631 CCGGG BpmI 661 CTCCAG CTGGAG(16/14) 1011 CTGGAG BsaI 1220 GAGACC GGTCTC(1/5) 1038 GGTCTC BsrDI 389 CATTGC GCAATG(2/0) 602 CATTGC BstF5I 4 GGATG GGATG(2/0) 779 GGATG EarI 273 CTCTTC CTCTTC(1/4) 597 CTCTTC FokI 4 GGATG GGATG(9/13) 779 GGATG Ksp632I 273 CTCTTC CTCTTC(1/4) 597 CTCTTC MluNI 84 TGGCCA TGG^CCA 1273 TGGCCA MscI 84 TGGCCA TGG^CCA 1273 TGGCCA NciI 630 CCCGG CC^[GC]GG 631 CCGGG NspI 172 ACATGT [GA]CATG^[CT] 668 GCATGC SexAI 478 ACCTGGT A^CC[AT]GGT 751 ACCTGGT SfaNI 5 GATGC GCATC(5/9) 780 GATGC SfcI 132 CTGCAG C^T[GA][CT]AG 1105 CTGTAG SmlI 920 CTTAAG C^T[CT][GA]AG 1139 CTCAAG ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AciI AfaI AluI AvaII BbvI BfaI BmyI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstNI Cac8I Cfr13I DdeI DraII EaeI EcoO109I EcoRII FauI Fnu4HI HaeIII HapII HpaII HphI ItaI MaeI MaeIII MboII MnlI MseI MspA1I MspI MvaI MwoI NlaIII NlaIV PpuMI RsaI Sau96I ScrFI Tru9I TseI Tsp45I Tsp509I TspRI