##################################
# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AgeI Alw44I AlwI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AviII BamHI BanI BbeI
BbrPI BclI BglI BglII BlpI
Bpu1102I BsaAI BsaBI BsaHI BsiEI
BsiWI BsmI Bsp1407I BspDI BspHI
BsrBI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstUI BstXI BstYI
BstZ17I CelII Cfr10I ClaI DpnII
DraI DraIII DrdI EagI EclXI
Eco47III Eco52I EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeII MamI MboI MflI
MluI MvnI NaeI NarI NcoI
NdeI NdeII NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
SacI SacII SalI SapI Sau3AI
SbfI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI TaqI TfiI
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmnI
###################################
# ONE CUT ENZYMES ( 64 enzymes) #
###################################
AccI 495 GTCTAC GT^[AC][GT]AC
AccIII 189 TCCGGA T^CCGGA
AcsI 522 GAATTC [GA]^AATT[CT]
AflII 920 CTTAAG C^TTAAG
AflIII 172 ACATGT A^C[GA][CT]GT
AhdI 32 GACGGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 581 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1091 CCTCAGG CC^T[ACGT]AGG
ApoI 522 GAATTC [GA]^AATT[CT]
AspEI 32 GACGGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 417 GTGCTC G[AT]GC[AT]^C
AspI 477 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 630 CCCGGG C^[CT]CG[GA]G
AvrII 382 CCTAGG C^CTAGG
BanII 1148 GGGCTC G[GA]GC[CT]^C
BbsI 345 GTCTTC GAAGAC(2/6)
BbvCI 8 GCTGAGG CCTCAGC(-5/-2)
BcgI 309 CGACTGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 756 GTATCC GTATCC(6/5)
BfrI 920 CTTAAG C^TTAAG
BlnI 382 CCTAGG C^CTAGG
BpuAI 345 GTCTTC GAAGAC(2/6)
BsaWI 189 TCCGGA [AT]^CCGG[AT]
BseAI 189 TCCGGA T^CCGGA
BseRI 260 CTCCTC GAGGAG(10/8)
BsgI 510 CTGCAC GTGCAG(16/14)
BsiHKAI 417 GTGCTC G[AT]GC[AT]^C
BsmBI 818 GAGACG CGTCTC(1/5)
BsoBI 630 CCCGGG C^[CT]CG[GA]G
BspEI 189 TCCGGA T^CCGGA
BspLU11I 172 ACATGT A^CATGT
BspMI 164 GCAGGT ACCTGC(4/8)
BssSI 828 CTCGTG CACGAG(-5/-1)
BstEII 1096 GGTCACC G^GT[ACGT]ACC
BstPI 1096 GGTCACC G^GT[ACGT]ACC
Bsu36I 1091 CCTCAGG CC^T[ACGT]AGG
CfoI 430 GCGC GCG^C
CpoI 562 CGGACCG CG^G[AT]CCG
DsaI 556 CCGTGG C^C[GA][CT]GG
Eam1105I 32 GACGGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1091 CCTCAGG CC^T[ACGT]AGG
EcoNI 1122 CCTGACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1096 GGTCACC G^GT[ACGT]ACC
EcoRI 522 GAATTC G^AATTC
EcoT14I 382 CCTAGG C^C[AT][AT]GG
HaeII 429 AGCGCC [GA]GCGC^[CT]
HhaI 430 GCGC GCG^C
HinP1I 430 GCGC G^CGC
HinfI 810 GACTC G^A[ACGT]TC
MfeI 899 CAATTG C^AATTG
MroI 189 TCCGGA T^CCGGA
MslI 70 CATCATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 899 CAATTG C^AATTG
PflFI 477 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 810 GACTC GAGTC(4/5)
PstI 132 CTGCAG CTGCA^G
PvuII 848 CAGCTG CAG^CTG
RsrII 562 CGGACCG CG^G[AT]CCG
SmaI 630 CCCGGG CCC^GGG
SphI 668 GCATGC GCATG^C
StyI 382 CCTAGG C^C[AT][AT]GG
Tth111I 477 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 630 CCCGGG C^CCGGG
XmaI 630 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BalI 84 TGGCCA TGG^CCA
1273 TGGCCA
BcnI 630 CCCGG CC^[GC]GG
631 CCGGG
BpmI 661 CTCCAG CTGGAG(16/14)
1011 CTGGAG
BsaI 1220 GAGACC GGTCTC(1/5)
1038 GGTCTC
BsrDI 389 CATTGC GCAATG(2/0)
602 CATTGC
BstF5I 4 GGATG GGATG(2/0)
779 GGATG
EarI 273 CTCTTC CTCTTC(1/4)
597 CTCTTC
FokI 4 GGATG GGATG(9/13)
779 GGATG
Ksp632I 273 CTCTTC CTCTTC(1/4)
597 CTCTTC
MluNI 84 TGGCCA TGG^CCA
1273 TGGCCA
MscI 84 TGGCCA TGG^CCA
1273 TGGCCA
NciI 630 CCCGG CC^[GC]GG
631 CCGGG
NspI 172 ACATGT [GA]CATG^[CT]
668 GCATGC
SexAI 478 ACCTGGT A^CC[AT]GGT
751 ACCTGGT
SfaNI 5 GATGC GCATC(5/9)
780 GATGC
SfcI 132 CTGCAG C^T[GA][CT]AG
1105 CTGTAG
SmlI 920 CTTAAG C^T[CT][GA]AG
1139 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BfaI BmyI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstNI Cac8I Cfr13I DdeI DraII
EaeI EcoO109I EcoRII FauI Fnu4HI
HaeIII HapII HpaII HphI ItaI
MaeI MaeIII MboII MnlI MseI
MspA1I MspI MvaI MwoI NlaIII
NlaIV PpuMI RsaI Sau96I ScrFI
Tru9I TseI Tsp45I Tsp509I TspRI