##################################
# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AocI
Aor51HI ApaI AscI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV EcoT22I FbaI
FseI FspI HaeII HincII HindII
HindIII HpaI HphI KasI KpnI
MaeII MamI MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfoI SgrAI SmaI SnaBI
Sse8387I SspBI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AcsI 225 GAATTC [GA]^AATT[CT]
AfaI 359 GTAC GT^AC
Alw44I 664 GTGCAC G^TGCAC
AlwNI 231 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 664 GTGCAC G^TGCAC
ApoI 225 GAATTC [GA]^AATT[CT]
AseI 108 ATTAAT AT^TAAT
AsnI 108 ATTAAT AT^TAAT
AspHI 664 GTGCAC G[AT]GC[AT]^C
AvaI 38 CCCGAG C^[CT]CG[GA]G
BamHI 34 GGATCC G^GATCC
BanI 580 GGTGCC G^G[CT][GA]CC
BanII 735 GAGCCC G[GA]GC[CT]^C
BpmI 842 CTGGAG CTGGAG(16/14)
BseRI 676 CTCCTC GAGGAG(10/8)
BsgI 390 GTGCAG GTGCAG(16/14)
BsiHKAI 664 GTGCAC G[AT]GC[AT]^C
BsmAI 700 GAGAC GTCTC(1/5)
BsmI 593 GAATGC GAATGC(1/-1)
BsoBI 38 CCCGAG C^[CT]CG[GA]G
BstBI 633 TTCGAA TT^CGAA
BstXI 776 CCAGCTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 34 GGATCC [GA]^GATC[CT]
CfoI 723 GCGC GCG^C
DraII 62 GGGACCC [GA]G^G[ACGT]CC[CT]
EarI 689 GAAGAG CTCTTC(1/4)
EcoNI 138 CCTTTGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 62 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 225 GAATTC G^AATTC
HapII 190 CCGG C^CGG
HhaI 723 GCGC GCG^C
HinP1I 723 GCGC G^CGC
HpaII 190 CCGG C^CGG
Ksp632I 689 GAAGAG CTCTTC(1/4)
KspI 598 CCGCGG CCGC^GG
MflI 34 GGATCC [GA]^GATC[CT]
MspA1I 598 CCGCGG C[AC]G^C[GT]G
MspI 190 CCGG C^CGG
NspV 633 TTCGAA TT^CGAA
PleI 469 GAGTC GAGTC(4/5)
PpuMI 62 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 108 ATTAAT AT^TAAT
RsaI 359 GTAC GT^AC
SacII 598 CCGCGG CCGC^GG
SfcI 281 CTACAG C^T[GA][CT]AG
SfuI 633 TTCGAA TT^CGAA
SmlI 91 CTTGAG C^T[CT][GA]AG
SpeI 374 ACTAGT A^CTAGT
SphI 215 GCATGC GCATG^C
SspI 867 AATATT AAT^ATT
StuI 125 AGGCCT AGG^CCT
TaqI 634 TCGA T^CGA
TthHB8I 634 TCGA T^CGA
XhoII 34 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 203 GACGCC G[GA]^CG[CT]C
571 GGCGTC
AluI 162 AGCT AG^CT
778 AGCT
BbsI 493 GTCTTC GAAGAC(2/6)
656 GTCTTC
BfaI 240 CTAG C^TAG
375 CTAG
BglI 189 GCCGGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
597 GCCGCGGGGGC
BpuAI 493 GTCTTC GAAGAC(2/6)
656 GTCTTC
BsaHI 203 GACGCC G[GA]^CG[CT]C
571 GGCGTC
BspMI 445 ACCTGC ACCTGC(4/8)
392 GCAGGT
BstF5I 535 CATCC GGATG(2/0)
756 CATCC
DdeI 733 CTGAG C^T[ACGT]AG
964 CTGAG
DpnII 35 GATC ^GATC
401 GATC
DsaI 567 CCACGG C^C[GA][CT]GG
598 CCGCGG
FokI 535 CATCC GGATG(9/13)
756 CATCC
HgaI 203 GACGC GACGC(5/10)
572 GCGTC
Hin1I 203 GACGCC G[GA]^CG[CT]C
571 GGCGTC
MaeI 240 CTAG C^TAG
375 CTAG
MaeIII 25 GTGAC ^GT[ACGT]AC
922 GTGAC
MboI 35 GATC ^GATC
401 GATC
NdeII 35 GATC ^GATC
401 GATC
NspI 17 ACATGC [GA]CATG^[CT]
215 GCATGC
Sau3AI 35 GATC ^GATC
401 GATC
Tsp45I 25 GTGAC ^GT[GC]AC
922 GTGAC
Tsp509I 111 AATT ^AATT
226 AATT
TspRI 617 CAGTG CA[GC]TG[ACGT][ACGT]^
662 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AccII AciI AlwI AvaII BbvI
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstNI BstUI
Cac8I Cfr13I EcoRII EcoT14I FauI
Fnu4HI HaeIII HinfI ItaI MboII
MnlI MseI MslI MvaI MvnI
MwoI NlaIII NlaIV Sau96I ScrFI
StyI TfiI Tru9I TseI