################################## # NO CUT ENZYMES ( 93 enzymes) # ################################## Acc65I AccIII AclI AflII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspI AviII AvrII BbrPI BbsI BcgI BciVI BclI BfrI BglII BlnI BpuAI BsaAI BsaWI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI BstBI BstXI Bsu36I ClaI CpoI DraI Eco47III Eco81I EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI MluI MroI MseI NdeI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SalI SexAI SfiI SfuI SmaI SnaBI SpeI SphI SspBI SwaI Tru9I Tth111I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AatII 340 GACGTC GACGT^C AccI 1601 GTATAC GT^[AC][GT]AC AflIII 1036 ACGTGT A^C[GA][CT]GT Asp700I 794 GAACGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 1406 GAGCTC G[AT]GC[AT]^C BalI 897 TGGCCA TGG^CCA BamHI 831 GGATCC G^GATCC BbeI 554 GGCGCC GGCGC^C BglI 223 GCCGGAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1325 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1325 GCTGAGC GC^T[ACGT]AGC BsaBI 949 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 683 GAGACC GGTCTC(1/5) BsgI 735 CTGCAC GTGCAG(16/14) BsiHKAI 1406 GAGCTC G[AT]GC[AT]^C BsmI 637 GCATTC GAATGC(1/-1) BsrBI 77 GAGCGG CCGCTC(-3/-3) Bst1107I 1601 GTATAC GTA^TAC BstEII 442 GGTGACC G^GT[ACGT]ACC BstPI 442 GGTGACC G^GT[ACGT]ACC BstZ17I 1601 GTATAC GTA^TAC CelII 1325 GCTGAGC GC^T[ACGT]AGC DraIII 437 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1103 GACGTGAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 442 GGTGACC G^GT[ACGT]ACC EcoRI 1565 GAATTC G^AATTC KasI 554 GGCGCC G^GCGCC MamI 949 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1684 CAATTG C^AATTG MluNI 897 TGGCCA TGG^CCA MscI 897 TGGCCA TGG^CCA MslI 738 CACCTCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 1684 CAATTG C^AATTG NaeI 116 GCCGGC GCC^GGC NarI 554 GGCGCC GG^CGCC NcoI 1366 CCATGG C^CATGG NgoMIV 116 GCCGGC G^CCGGC NheI 778 GCTAGC G^CTAGC PshAI 685 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 1406 GAGCTC GAGCT^C SapI 536 GAAGAGC GCTCTTC(1/4) SbfI 513 CCTGCAGG CCTGCA^GG ScaI 1767 AGTACT AGT^ACT SfoI 554 GGCGCC GGC^GCC SgrAI 550 CACCGGCG C[GA]^CCGG[CT]G SmlI 1001 CTTGAG C^T[CT][GA]AG Sse8387I 513 CCTGCAGG CCTGCA^GG SspI 981 AATATT AAT^ATT StuI 1096 AGGCCT AGG^CCT TfiI 1707 GAATC G^A[AT]TC XbaI 1171 TCTAGA T^CTAGA XmnI 794 GAACGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AhdI 335 GACCCGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 386 GACGGCGCGTC AspEI 335 GACCCGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 386 GACGGCGCGTC BbvCI 106 GCTGAGG CCTCAGC(-5/-2) 254 GCTGAGG BsmAI 683 GAGAC GTCTC(1/5) 1595 GAGAC BspMI 512 ACCTGC ACCTGC(4/8) 633 ACCTGC BsrFI 116 GCCGGC [GA]^CCGG[CT] 551 ACCGGC BstAPI 564 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1081 GCACAAACTGC BstYI 831 GGATCC [GA]^GATC[CT] 1588 AGATCC Cfr10I 116 GCCGGC [GA]^CCGG[CT] 551 ACCGGC Eam1105I 335 GACCCGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 386 GACGGCGCGTC EarI 536 GAAGAG CTCTTC(1/4) 1742 GAAGAG EcoNI 39 CCTGGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 422 CCTTCAAGAGG EcoT14I 1366 CCATGG C^C[AT][AT]GG 1486 CCAAGG HincII 1461 GTCAAC GT[CT]^[GA]AC 1611 GTCAAC HindII 1461 GTCAAC GT[CT]^[GA]AC 1611 GTCAAC Ksp632I 536 GAAGAG CTCTTC(1/4) 1742 GAAGAG KspI 121 CCGCGG CCGC^GG 471 CCGCGG MflI 831 GGATCC [GA]^GATC[CT] 1588 AGATCC SacII 121 CCGCGG CCGC^GG 471 CCGCGG SfaNI 360 GATGC GCATC(5/9) 1064 GATGC StyI 1366 CCATGG C^C[AT][AT]GG 1486 CCAAGG TaqI 398 TCGA T^CGA 1137 TCGA TthHB8I 398 TCGA T^CGA 1137 TCGA XcmI 868 CCAGAATGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1528 CCACAATGTAGATGG XhoII 831 GGATCC [GA]^GATC[CT] 1588 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AvaI AvaII BanI BanII BbvI BcnI BfaI BmyI BpmI BsaHI BsaJI BseRI BsiEI BsiYI BslI BsmFI BsoBI Bsp1286I BsrI BssHII BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfcI TseI Tsp45I Tsp509I TspRI