##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspI
AviII AvrII BbrPI BbsI BcgI
BciVI BclI BfrI BglII BlnI
BpuAI BsaAI BsaWI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssSI BstBI
BstXI Bsu36I ClaI CpoI DraI
Eco47III Eco81I EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
MluI MroI MseI NdeI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SalI SexAI SfiI
SfuI SmaI SnaBI SpeI SphI
SspBI SwaI Tru9I Tth111I Van91I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 340 GACGTC GACGT^C
AccI 1601 GTATAC GT^[AC][GT]AC
AflIII 1036 ACGTGT A^C[GA][CT]GT
Asp700I 794 GAACGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1406 GAGCTC G[AT]GC[AT]^C
BalI 897 TGGCCA TGG^CCA
BamHI 831 GGATCC G^GATCC
BbeI 554 GGCGCC GGCGC^C
BglI 223 GCCGGAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1325 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1325 GCTGAGC GC^T[ACGT]AGC
BsaBI 949 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 683 GAGACC GGTCTC(1/5)
BsgI 735 CTGCAC GTGCAG(16/14)
BsiHKAI 1406 GAGCTC G[AT]GC[AT]^C
BsmI 637 GCATTC GAATGC(1/-1)
BsrBI 77 GAGCGG CCGCTC(-3/-3)
Bst1107I 1601 GTATAC GTA^TAC
BstEII 442 GGTGACC G^GT[ACGT]ACC
BstPI 442 GGTGACC G^GT[ACGT]ACC
BstZ17I 1601 GTATAC GTA^TAC
CelII 1325 GCTGAGC GC^T[ACGT]AGC
DraIII 437 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1103 GACGTGAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 442 GGTGACC G^GT[ACGT]ACC
EcoRI 1565 GAATTC G^AATTC
KasI 554 GGCGCC G^GCGCC
MamI 949 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1684 CAATTG C^AATTG
MluNI 897 TGGCCA TGG^CCA
MscI 897 TGGCCA TGG^CCA
MslI 738 CACCTCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1684 CAATTG C^AATTG
NaeI 116 GCCGGC GCC^GGC
NarI 554 GGCGCC GG^CGCC
NcoI 1366 CCATGG C^CATGG
NgoMIV 116 GCCGGC G^CCGGC
NheI 778 GCTAGC G^CTAGC
PshAI 685 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1406 GAGCTC GAGCT^C
SapI 536 GAAGAGC GCTCTTC(1/4)
SbfI 513 CCTGCAGG CCTGCA^GG
ScaI 1767 AGTACT AGT^ACT
SfoI 554 GGCGCC GGC^GCC
SgrAI 550 CACCGGCG C[GA]^CCGG[CT]G
SmlI 1001 CTTGAG C^T[CT][GA]AG
Sse8387I 513 CCTGCAGG CCTGCA^GG
SspI 981 AATATT AAT^ATT
StuI 1096 AGGCCT AGG^CCT
TfiI 1707 GAATC G^A[AT]TC
XbaI 1171 TCTAGA T^CTAGA
XmnI 794 GAACGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AhdI 335 GACCCGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
386 GACGGCGCGTC
AspEI 335 GACCCGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
386 GACGGCGCGTC
BbvCI 106 GCTGAGG CCTCAGC(-5/-2)
254 GCTGAGG
BsmAI 683 GAGAC GTCTC(1/5)
1595 GAGAC
BspMI 512 ACCTGC ACCTGC(4/8)
633 ACCTGC
BsrFI 116 GCCGGC [GA]^CCGG[CT]
551 ACCGGC
BstAPI 564 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1081 GCACAAACTGC
BstYI 831 GGATCC [GA]^GATC[CT]
1588 AGATCC
Cfr10I 116 GCCGGC [GA]^CCGG[CT]
551 ACCGGC
Eam1105I 335 GACCCGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
386 GACGGCGCGTC
EarI 536 GAAGAG CTCTTC(1/4)
1742 GAAGAG
EcoNI 39 CCTGGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
422 CCTTCAAGAGG
EcoT14I 1366 CCATGG C^C[AT][AT]GG
1486 CCAAGG
HincII 1461 GTCAAC GT[CT]^[GA]AC
1611 GTCAAC
HindII 1461 GTCAAC GT[CT]^[GA]AC
1611 GTCAAC
Ksp632I 536 GAAGAG CTCTTC(1/4)
1742 GAAGAG
KspI 121 CCGCGG CCGC^GG
471 CCGCGG
MflI 831 GGATCC [GA]^GATC[CT]
1588 AGATCC
SacII 121 CCGCGG CCGC^GG
471 CCGCGG
SfaNI 360 GATGC GCATC(5/9)
1064 GATGC
StyI 1366 CCATGG C^C[AT][AT]GG
1486 CCAAGG
TaqI 398 TCGA T^CGA
1137 TCGA
TthHB8I 398 TCGA T^CGA
1137 TCGA
XcmI 868 CCAGAATGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1528 CCACAATGTAGATGG
XhoII 831 GGATCC [GA]^GATC[CT]
1588 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AvaI
AvaII BanI BanII BbvI BcnI
BfaI BmyI BpmI BsaHI BsaJI
BseRI BsiEI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrI BssHII BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EclXI Eco52I EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfcI TseI
Tsp45I Tsp509I TspRI