##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI AhdI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BamHI BbrPI
BbvCI BcgI BclI BglII BlnI
BlpI Bpu1102I BseAI BsiEI BsmBI
BspEI BspHI BspLU11I BssHII Bst1107I
BstAPI BstBI BstZ17I Bsu36I CelII
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI HindIII HpaI KpnI
KspI MfeI MluI MroI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI TfiI
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
AccI 793 GTAGAC GT^[AC][GT]AC
AccII 9 CGCG CG^CG
AcsI 1131 AAATTT [GA]^AATT[CT]
AcyI 840 GGCGCC G[GA]^CG[CT]C
AflII 1901 CTTAAG C^TTAAG
Alw44I 748 GTGCAC G^TGCAC
AlwNI 199 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 748 GTGCAC G^TGCAC
ApoI 1131 AAATTT [GA]^AATT[CT]
AspI 1649 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 87 GGACC G^G[AT]CC
BbeI 840 GGCGCC GGCGC^C
BciVI 1892 GTATCC GTATCC(6/5)
BfrI 1901 CTTAAG C^TTAAG
BsaAI 660 CACGTA [CT]AC^GT[GA]
BsaBI 810 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 840 GGCGCC G[GA]^CG[CT]C
BsaI 922 GAGACC GGTCTC(1/5)
BsiWI 662 CGTACG C^GTACG
Bsp1407I 1168 TGTACA T^GTACA
BspDI 73 ATCGAT AT^CGAT
BspMI 512 ACCTGC ACCTGC(4/8)
BsrBI 400 GAGCGG CCGCTC(-3/-3)
BsrDI 1607 CATTGC GCAATG(2/0)
BsrFI 4 ACCGGC [GA]^CCGG[CT]
BsrGI 1168 TGTACA T^GTACA
BstEII 1871 GGTGACC G^GT[ACGT]ACC
BstPI 1871 GGTGACC G^GT[ACGT]ACC
BstUI 9 CGCG CG^CG
Cfr10I 4 ACCGGC [GA]^CCGG[CT]
ClaI 73 ATCGAT AT^CGAT
DraII 1003 GGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 88 GACCGCTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 1003 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1871 GGTGACC G^GT[ACGT]ACC
HaeII 840 GGCGCC [GA]GCGC^[CT]
Hin1I 840 GGCGCC G[GA]^CG[CT]C
HincII 508 GTCAAC GT[CT]^[GA]AC
HindII 508 GTCAAC GT[CT]^[GA]AC
KasI 840 GGCGCC G^GCGCC
MamI 810 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 1902 TTAA T^TAA
MvnI 9 CGCG CG^CG
NarI 840 GGCGCC GG^CGCC
NspI 861 GCATGC [GA]CATG^[CT]
PflFI 1649 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 198 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1143 CTGCAG CTGCA^G
SacI 565 GAGCTC GAGCT^C
SapI 1234 GAAGAGC GCTCTTC(1/4)
SfcI 1143 CTGCAG C^T[GA][CT]AG
SfoI 840 GGCGCC GGC^GCC
SmaI 819 CCCGGG CCC^GGG
SmlI 1901 CTTAAG C^T[CT][GA]AG
SphI 861 GCATGC GCATG^C
SspBI 1168 TGTACA T^GTACA
StuI 555 AGGCCT AGG^CCT
Tru9I 1902 TTAA T^TAA
Tth111I 1649 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 198 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 448 CCAGTGGCCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 819 CCCGGG C^CCGGG
XmaI 819 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AvaI 819 CCCGGG C^[CT]CG[GA]G
1538 CTCGGG
BalI 119 TGGCCA TGG^CCA
1687 TGGCCA
BfaI 295 CTAG C^TAG
1910 CTAG
BglI 524 GCCTGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
668 GCCTCAGAGGC
BsaWI 1012 TCCGGT [AT]^CCGG[AT]
1089 ACCGGA
BsgI 931 CTGCAC GTGCAG(16/14)
346 GTGCAG
BsmI 465 GAATGC GAATGC(1/-1)
703 GAATGC
BsoBI 819 CCCGGG C^[CT]CG[GA]G
1538 CTCGGG
BssSI 751 CACGAG CACGAG(-5/-1)
1072 CTCGTG
HinfI 541 GAGTC G^A[ACGT]TC
1756 GAGTC
MaeI 295 CTAG C^TAG
1910 CTAG
MluNI 119 TGGCCA TGG^CCA
1687 TGGCCA
MscI 119 TGGCCA TGG^CCA
1687 TGGCCA
PleI 541 GAGTC GAGTC(4/5)
1756 GAGTC
Tsp509I 628 AATT ^AATT
1132 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
BanI BanII BbsI BbvI BcnI
BmyI BpmI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EaeI EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TseI Tsp45I TspRI
TthHB8I XhoII