################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AvaI AviII AvrII BalI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BseAI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspMI BsrBI BsrFI BssHII Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII Hin1I HincII HindII HpaI KasI KpnI MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacI SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StyI SwaI XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AlwNI 77 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 1706 ATTAAT AT^TAAT AsnI 1706 ATTAAT AT^TAAT AspI 768 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 96 GGATCC G^GATCC BglII 622 AGATCT A^GATCT BpmI 917 CTCCAG CTGGAG(16/14) BsaWI 1740 TCCGGT [AT]^CCGG[AT] BsiEI 296 CGACCG CG[GA][CT]^CG Bsp1407I 1152 TGTACA T^GTACA BspLU11I 1652 ACATGT A^CATGT BsrDI 1627 GCAATG GCAATG(2/0) BsrGI 1152 TGTACA T^GTACA BssSI 87 CACGAG CACGAG(-5/-1) BstAPI 463 GCAGCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraII 820 GGGTCCT [GA]G^G[ACGT]CC[CT] DsaI 53 CCGCGG C^C[GA][CT]GG EaeI 410 CGGCCA [CT]^GGCC[GA] EcoNI 182 CCTTATAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 820 GGGTCCT [GA]G^G[ACGT]CC[CT] HgaI 874 GCGTC GACGC(5/10) HindIII 1351 AAGCTT A^AGCTT KspI 53 CCGCGG CCGC^GG MaeII 1577 ACGT A^CGT NheI 450 GCTAGC G^CTAGC NspI 1652 ACATGT [GA]CATG^[CT] PflFI 768 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1322 CCAAAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 820 GGGTCCT [GA]G^G[AT]CC[CT] PshBI 1706 ATTAAT AT^TAAT PstI 461 CTGCAG CTGCA^G PvuII 464 CAGCTG CAG^CTG SacII 53 CCGCGG CCGC^GG SapI 355 GAAGAGC GCTCTTC(1/4) SspBI 1152 TGTACA T^GTACA StuI 1449 AGGCCT AGG^CCT TaqI 1264 TCGA T^CGA Tth111I 768 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1264 TCGA T^CGA Van91I 1322 CCAAAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AfaI 92 GTAC GT^AC 1153 GTAC AflIII 1577 ACGTGT A^C[GA][CT]GT 1652 ACATGT AspHI 320 GAGCAC G[AT]GC[AT]^C 1469 GTGCTC BanII 192 GAGCCC G[GA]GC[CT]^C 711 GGGCTC BcnI 344 CCCGG CC^[GC]GG 836 CCGGG BsaI 1396 GAGACC GGTCTC(1/5) 1331 GGTCTC BseRI 189 GAGGAG GAGGAG(10/8) 1569 GAGGAG BsgI 776 CTGCAC GTGCAG(16/14) 37 GTGCAG BsiHKAI 320 GAGCAC G[AT]GC[AT]^C 1469 GTGCTC BsmFI 1574 GGGAC GGGAC(10/14) 1723 GGGAC CfoI 269 GCGC GCG^C 348 GCGC EarI 26 GAAGAG CTCTTC(1/4) 355 GAAGAG HhaI 269 GCGC GCG^C 348 GCGC HinP1I 269 GCGC G^CGC 348 GCGC Ksp632I 26 GAAGAG CTCTTC(1/4) 355 GAAGAG MspA1I 53 CCGCGG C[AC]G^C[GT]G 464 CAGCTG NciI 344 CCCGG CC^[GC]GG 836 CCGGG PleI 600 GACTC GAGTC(4/5) 1368 GAGTC RsaI 92 GTAC GT^AC 1153 GTAC SfcI 125 CTATAG C^T[GA][CT]AG 461 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AccII AciI AcsI AluI AlwI ApoI AvaII BbsI BbvI BfaI BmyI BpuAI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I Cfr13I DdeI DpnII EcoRII FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MvnI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI SmlI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII