##################################
# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspEI AvaI AviII
AvrII BalI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BseAI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BspHI
BspMI BsrBI BsrFI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HaeII Hin1I HincII
HindII HpaI KasI KpnI MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NcoI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RcaI
RsrII SacI SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StyI SwaI XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AlwNI 77 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 1706 ATTAAT AT^TAAT
AsnI 1706 ATTAAT AT^TAAT
AspI 768 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 96 GGATCC G^GATCC
BglII 622 AGATCT A^GATCT
BpmI 917 CTCCAG CTGGAG(16/14)
BsaWI 1740 TCCGGT [AT]^CCGG[AT]
BsiEI 296 CGACCG CG[GA][CT]^CG
Bsp1407I 1152 TGTACA T^GTACA
BspLU11I 1652 ACATGT A^CATGT
BsrDI 1627 GCAATG GCAATG(2/0)
BsrGI 1152 TGTACA T^GTACA
BssSI 87 CACGAG CACGAG(-5/-1)
BstAPI 463 GCAGCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraII 820 GGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 53 CCGCGG C^C[GA][CT]GG
EaeI 410 CGGCCA [CT]^GGCC[GA]
EcoNI 182 CCTTATAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 820 GGGTCCT [GA]G^G[ACGT]CC[CT]
HgaI 874 GCGTC GACGC(5/10)
HindIII 1351 AAGCTT A^AGCTT
KspI 53 CCGCGG CCGC^GG
MaeII 1577 ACGT A^CGT
NheI 450 GCTAGC G^CTAGC
NspI 1652 ACATGT [GA]CATG^[CT]
PflFI 768 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1322 CCAAAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 820 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 1706 ATTAAT AT^TAAT
PstI 461 CTGCAG CTGCA^G
PvuII 464 CAGCTG CAG^CTG
SacII 53 CCGCGG CCGC^GG
SapI 355 GAAGAGC GCTCTTC(1/4)
SspBI 1152 TGTACA T^GTACA
StuI 1449 AGGCCT AGG^CCT
TaqI 1264 TCGA T^CGA
Tth111I 768 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1264 TCGA T^CGA
Van91I 1322 CCAAAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 92 GTAC GT^AC
1153 GTAC
AflIII 1577 ACGTGT A^C[GA][CT]GT
1652 ACATGT
AspHI 320 GAGCAC G[AT]GC[AT]^C
1469 GTGCTC
BanII 192 GAGCCC G[GA]GC[CT]^C
711 GGGCTC
BcnI 344 CCCGG CC^[GC]GG
836 CCGGG
BsaI 1396 GAGACC GGTCTC(1/5)
1331 GGTCTC
BseRI 189 GAGGAG GAGGAG(10/8)
1569 GAGGAG
BsgI 776 CTGCAC GTGCAG(16/14)
37 GTGCAG
BsiHKAI 320 GAGCAC G[AT]GC[AT]^C
1469 GTGCTC
BsmFI 1574 GGGAC GGGAC(10/14)
1723 GGGAC
CfoI 269 GCGC GCG^C
348 GCGC
EarI 26 GAAGAG CTCTTC(1/4)
355 GAAGAG
HhaI 269 GCGC GCG^C
348 GCGC
HinP1I 269 GCGC G^CGC
348 GCGC
Ksp632I 26 GAAGAG CTCTTC(1/4)
355 GAAGAG
MspA1I 53 CCGCGG C[AC]G^C[GT]G
464 CAGCTG
NciI 344 CCCGG CC^[GC]GG
836 CCGGG
PleI 600 GACTC GAGTC(4/5)
1368 GAGTC
RsaI 92 GTAC GT^AC
1153 GTAC
SfcI 125 CTATAG C^T[GA][CT]AG
461 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
ApoI AvaII BbsI BbvI BfaI
BmyI BpuAI BsaJI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I Cfr13I
DdeI DpnII EcoRII FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI SmlI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII