##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI ApaI AscI Asp718I AspEI
AvrII BamHI BbeI BbrPI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaWI BseAI BsgI BsiWI Bsp1407I
BspDI BspEI BsrFI BsrGI BssHII
Bst1107I BstBI BstZ17I CelII Cfr10I
ClaI CpoI DrdI Eam1105I EcoNI
EcoRV FseI HincII HindII HpaI
KasI KpnI MaeII MamI MfeI
MluI MroI MunI NaeI NarI
NgoMIV NruI NspV PacI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I SspBI SspI StuI
SwaI XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 2810 GTCTAC GT^[AC][GT]AC
AcsI 310 GAATTC [GA]^AATT[CT]
AcyI 1932 GACGCC G[GA]^CG[CT]C
AfaI 1320 GTAC GT^AC
Alw44I 162 GTGCAC G^TGCAC
AocI 1901 CCTCAGG CC^T[ACGT]AGG
Aor51HI 731 AGCGCT AGC^GCT
ApaLI 162 GTGCAC G^TGCAC
ApoI 310 GAATTC [GA]^AATT[CT]
AvaI 1756 CTCGAG C^[CT]CG[GA]G
AviII 1382 TGCGCA TGC^GCA
BanI 1445 GGCACC G^G[CT][GA]CC
BbvCI 1926 GCTGAGG CCTCAGC(-5/-2)
BcgI 1293 GCAGCGAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 432 GTATCC GTATCC(6/5)
BglI 1 GCCGACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 1932 GACGCC G[GA]^CG[CT]C
BsmBI 2593 GAGACG CGTCTC(1/5)
BsmI 2093 GCATTC GAATGC(1/-1)
BsoBI 1756 CTCGAG C^[CT]CG[GA]G
BsrDI 1529 CATTGC GCAATG(2/0)
BssSI 1145 CTCGTG CACGAG(-5/-1)
Bsu36I 1901 CCTCAGG CC^T[ACGT]AGG
Eco47III 731 AGCGCT AGC^GCT
Eco81I 1901 CCTCAGG CC^T[ACGT]AGG
EcoRI 310 GAATTC G^AATTC
EcoT22I 386 ATGCAT ATGCA^T
FspI 1382 TGCGCA TGC^GCA
HaeII 731 AGCGCT [GA]GCGC^[CT]
Hin1I 1932 GACGCC G[GA]^CG[CT]C
KspI 38 CCGCGG CCGC^GG
NdeI 1630 CATATG CA^TATG
NheI 1035 GCTAGC G^CTAGC
NotI 34 GCGGCCGC GC^GGCCGC
NsiI 386 ATGCAT ATGCA^T
PaeR7I 1756 CTCGAG C^TCGAG
PflMI 1637 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 1320 GTAC GT^AC
SacII 38 CCGCGG CCGC^GG
SapI 1770 GAAGAGC GCTCTTC(1/4)
SphI 2272 GCATGC GCATG^C
Van91I 1637 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1756 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 43 enzymes) #
###################################
AflII 472 CTTAAG C^TTAAG
2925 CTTAAG
AflIII 1316 ACATGT A^C[GA][CT]GT
2800 ACATGT
AlwI 2821 GATCC GGATC(4/5)
780 GGATC
AseI 1674 ATTAAT AT^TAAT
2866 ATTAAT
AsnI 1674 ATTAAT AT^TAAT
2866 ATTAAT
Asp700I 931 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2567 GAATGATTTC
AspI 13 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
627 GACCGTGTC
BalI 638 TGGCCA TGG^CCA
2475 TGGCCA
BclI 1232 TGATCA T^GATCA
1715 TGATCA
BcnI 189 CCCGG CC^[GC]GG
1361 CCCGG
BfrI 472 CTTAAG C^TTAAG
2925 CTTAAG
BsaI 123 GGTCTC GGTCTC(1/5)
2040 GGTCTC
BsiEI 35 CGGCCG CG[GA][CT]^CG
2102 CGGCCG
BspHI 1514 TCATGA T^CATGA
1665 TCATGA
BspLU11I 1316 ACATGT A^CATGT
2800 ACATGT
BspMI 467 ACCTGC ACCTGC(4/8)
1254 GCAGGT
BstEII 1817 GGTCACC G^GT[ACGT]ACC
2699 GGTGACC
BstPI 1817 GGTCACC G^GT[ACGT]ACC
2699 GGTGACC
BstYI 780 GGATCT [GA]^GATC[CT]
2820 AGATCC
DraI 261 TTTAAA TTT^AAA
391 TTTAAA
DraIII 332 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
1794 CACAGAGTG
EagI 35 CGGCCG C^GGCCG
2102 CGGCCG
EclXI 35 CGGCCG C^GGCCG
2102 CGGCCG
Eco52I 35 CGGCCG C^GGCCG
2102 CGGCCG
EcoO65I 1817 GGTCACC G^GT[ACGT]ACC
2699 GGTGACC
FbaI 1232 TGATCA T^GATCA
1715 TGATCA
HgaI 1932 GACGC GACGC(5/10)
1008 GCGTC
MflI 780 GGATCT [GA]^GATC[CT]
2820 AGATCC
MluNI 638 TGGCCA TGG^CCA
2475 TGGCCA
MscI 638 TGGCCA TGG^CCA
2475 TGGCCA
NciI 189 CCCGG CC^[GC]GG
1361 CCCGG
NcoI 44 CCATGG C^CATGG
1541 CCATGG
PflFI 13 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
627 GACCGTGTC
PshBI 1674 ATTAAT AT^TAAT
2866 ATTAAT
RcaI 1514 TCATGA T^CATGA
1665 TCATGA
SacI 1018 GAGCTC GAGCT^C
1753 GAGCTC
SpeI 2183 ACTAGT A^CTAGT
2631 ACTAGT
TaqI 101 TCGA T^CGA
1757 TCGA
Tth111I 13 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
627 GACCGTGTC
TthHB8I 101 TCGA T^CGA
1757 TCGA
XcmI 1541 CCATGGCACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2258 CCACCACACACATGG
XhoII 780 GGATCT [GA]^GATC[CT]
2820 AGATCC
XmnI 931 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2567 GAATGATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AluI AlwNI AspHI
AvaII BanII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrBI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI