################################## # NO CUT ENZYMES ( 84 enzymes) # ################################## AatII Acc65I AccIII AclI AgeI AhdI ApaI AscI Asp718I AspEI AvrII BamHI BbeI BbrPI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsgI BsiWI Bsp1407I BspDI BspEI BsrFI BsrGI BssHII Bst1107I BstBI BstZ17I CelII Cfr10I ClaI CpoI DrdI Eam1105I EcoNI EcoRV FseI HincII HindII HpaI KasI KpnI MaeII MamI MfeI MluI MroI MunI NaeI NarI NgoMIV NruI NspV PacI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SspBI SspI StuI SwaI XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 2810 GTCTAC GT^[AC][GT]AC AcsI 310 GAATTC [GA]^AATT[CT] AcyI 1932 GACGCC G[GA]^CG[CT]C AfaI 1320 GTAC GT^AC Alw44I 162 GTGCAC G^TGCAC AocI 1901 CCTCAGG CC^T[ACGT]AGG Aor51HI 731 AGCGCT AGC^GCT ApaLI 162 GTGCAC G^TGCAC ApoI 310 GAATTC [GA]^AATT[CT] AvaI 1756 CTCGAG C^[CT]CG[GA]G AviII 1382 TGCGCA TGC^GCA BanI 1445 GGCACC G^G[CT][GA]CC BbvCI 1926 GCTGAGG CCTCAGC(-5/-2) BcgI 1293 GCAGCGAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 432 GTATCC GTATCC(6/5) BglI 1 GCCGACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 1932 GACGCC G[GA]^CG[CT]C BsmBI 2593 GAGACG CGTCTC(1/5) BsmI 2093 GCATTC GAATGC(1/-1) BsoBI 1756 CTCGAG C^[CT]CG[GA]G BsrDI 1529 CATTGC GCAATG(2/0) BssSI 1145 CTCGTG CACGAG(-5/-1) Bsu36I 1901 CCTCAGG CC^T[ACGT]AGG Eco47III 731 AGCGCT AGC^GCT Eco81I 1901 CCTCAGG CC^T[ACGT]AGG EcoRI 310 GAATTC G^AATTC EcoT22I 386 ATGCAT ATGCA^T FspI 1382 TGCGCA TGC^GCA HaeII 731 AGCGCT [GA]GCGC^[CT] Hin1I 1932 GACGCC G[GA]^CG[CT]C KspI 38 CCGCGG CCGC^GG NdeI 1630 CATATG CA^TATG NheI 1035 GCTAGC G^CTAGC NotI 34 GCGGCCGC GC^GGCCGC NsiI 386 ATGCAT ATGCA^T PaeR7I 1756 CTCGAG C^TCGAG PflMI 1637 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RsaI 1320 GTAC GT^AC SacII 38 CCGCGG CCGC^GG SapI 1770 GAAGAGC GCTCTTC(1/4) SphI 2272 GCATGC GCATG^C Van91I 1637 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 1756 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 43 enzymes) # ################################### AflII 472 CTTAAG C^TTAAG 2925 CTTAAG AflIII 1316 ACATGT A^C[GA][CT]GT 2800 ACATGT AlwI 2821 GATCC GGATC(4/5) 780 GGATC AseI 1674 ATTAAT AT^TAAT 2866 ATTAAT AsnI 1674 ATTAAT AT^TAAT 2866 ATTAAT Asp700I 931 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2567 GAATGATTTC AspI 13 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 627 GACCGTGTC BalI 638 TGGCCA TGG^CCA 2475 TGGCCA BclI 1232 TGATCA T^GATCA 1715 TGATCA BcnI 189 CCCGG CC^[GC]GG 1361 CCCGG BfrI 472 CTTAAG C^TTAAG 2925 CTTAAG BsaI 123 GGTCTC GGTCTC(1/5) 2040 GGTCTC BsiEI 35 CGGCCG CG[GA][CT]^CG 2102 CGGCCG BspHI 1514 TCATGA T^CATGA 1665 TCATGA BspLU11I 1316 ACATGT A^CATGT 2800 ACATGT BspMI 467 ACCTGC ACCTGC(4/8) 1254 GCAGGT BstEII 1817 GGTCACC G^GT[ACGT]ACC 2699 GGTGACC BstPI 1817 GGTCACC G^GT[ACGT]ACC 2699 GGTGACC BstYI 780 GGATCT [GA]^GATC[CT] 2820 AGATCC DraI 261 TTTAAA TTT^AAA 391 TTTAAA DraIII 332 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG 1794 CACAGAGTG EagI 35 CGGCCG C^GGCCG 2102 CGGCCG EclXI 35 CGGCCG C^GGCCG 2102 CGGCCG Eco52I 35 CGGCCG C^GGCCG 2102 CGGCCG EcoO65I 1817 GGTCACC G^GT[ACGT]ACC 2699 GGTGACC FbaI 1232 TGATCA T^GATCA 1715 TGATCA HgaI 1932 GACGC GACGC(5/10) 1008 GCGTC MflI 780 GGATCT [GA]^GATC[CT] 2820 AGATCC MluNI 638 TGGCCA TGG^CCA 2475 TGGCCA MscI 638 TGGCCA TGG^CCA 2475 TGGCCA NciI 189 CCCGG CC^[GC]GG 1361 CCCGG NcoI 44 CCATGG C^CATGG 1541 CCATGG PflFI 13 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 627 GACCGTGTC PshBI 1674 ATTAAT AT^TAAT 2866 ATTAAT RcaI 1514 TCATGA T^CATGA 1665 TCATGA SacI 1018 GAGCTC GAGCT^C 1753 GAGCTC SpeI 2183 ACTAGT A^CTAGT 2631 ACTAGT TaqI 101 TCGA T^CGA 1757 TCGA Tth111I 13 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 627 GACCGTGTC TthHB8I 101 TCGA T^CGA 1757 TCGA XcmI 1541 CCATGGCACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2258 CCACCACACACATGG XhoII 780 GGATCT [GA]^GATC[CT] 2820 AGATCC XmnI 931 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2567 GAATGATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AccII AciI AluI AlwNI AspHI AvaII BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrBI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI