##################################
# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI ApaI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BbeI
BbrPI BcgI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I CelII ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco52I EcoO65I EcoRI EcoT22I
FseI FspI HindIII KasI KpnI
MamI MfeI MluI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI SwaI
TfiI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccII 12 CGCG CG^CG
AcyI 960 GGCGTC G[GA]^CG[CT]C
Aor51HI 612 AGCGCT AGC^GCT
AvaI 1963 CCCGGG C^[CT]CG[GA]G
BanII 1137 GGGCTC G[GA]GC[CT]^C
BciVI 302 GTATCC GTATCC(6/5)
BsaHI 960 GGCGTC G[GA]^CG[CT]C
BsaI 1563 GGTCTC GGTCTC(1/5)
BsgI 1132 GTGCAG GTGCAG(16/14)
BsoBI 1963 CCCGGG C^[CT]CG[GA]G
BsrBI 1415 GAGCGG CCGCTC(-3/-3)
BsrFI 1274 ACCGGC [GA]^CCGG[CT]
BstAPI 394 GCAGTTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 12 CGCG CG^CG
Cfr10I 1274 ACCGGC [GA]^CCGG[CT]
DrdI 859 GACTTCACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1968 GAAGAG CTCTTC(1/4)
Eco47III 612 AGCGCT AGC^GCT
EcoNI 1110 CCTGCCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 960 GGCGTC G[GA]^CG[CT]C
HincII 1483 GTTAAC GT[CT]^[GA]AC
HindII 1483 GTTAAC GT[CT]^[GA]AC
HpaI 1483 GTTAAC GTT^AAC
Ksp632I 1968 GAAGAG CTCTTC(1/4)
KspI 11 CCGCGG CCGC^GG
MaeII 114 ACGT A^CGT
MvnI 12 CGCG CG^CG
PflMI 696 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1724 GGGACCT [GA]G^G[AT]CC[CT]
SacII 11 CCGCGG CCGC^GG
SapI 1968 GAAGAGC GCTCTTC(1/4)
SexAI 842 ACCAGGT A^CC[AT]GGT
SmaI 1963 CCCGGG CCC^GGG
TaqI 428 TCGA T^CGA
TthHB8I 428 TCGA T^CGA
Van91I 696 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1963 CCCGGG C^CCGGG
XmaI 1963 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccIII 752 TCCGGA T^CCGGA
1739 TCCGGA
Alw44I 1029 GTGCAC G^TGCAC
1774 GTGCAC
ApaLI 1029 GTGCAC G^TGCAC
1774 GTGCAC
Asp700I 153 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
477 GAATGGCTTC
AspHI 1029 GTGCAC G[AT]GC[AT]^C
1774 GTGCAC
BalI 966 TGGCCA TGG^CCA
2250 TGGCCA
BamHI 187 GGATCC G^GATCC
1736 GGATCC
BbvCI 2103 CCTCAGC CCTCAGC(-5/-2)
448 GCTGAGG
BfaI 763 CTAG C^TAG
1660 CTAG
BsaWI 752 TCCGGA [AT]^CCGG[AT]
1739 TCCGGA
BseAI 752 TCCGGA T^CCGGA
1739 TCCGGA
BsiHKAI 1029 GTGCAC G[AT]GC[AT]^C
1774 GTGCAC
BspEI 752 TCCGGA T^CCGGA
1739 TCCGGA
BspMI 771 GCAGGT ACCTGC(4/8)
1422 GCAGGT
DraII 1328 AGGCCCT [GA]G^G[ACGT]CC[CT]
1724 GGGACCT
EcoO109I 1328 AGGCCCT [GA]G^G[ACGT]CC[CT]
1724 GGGACCT
EcoRV 73 GATATC GAT^ATC
81 GATATC
HaeII 612 AGCGCT [GA]GCGC^[CT]
1222 AGCGCC
HgaI 961 GCGTC GACGC(5/10)
1358 GCGTC
MaeI 763 CTAG C^TAG
1660 CTAG
MluNI 966 TGGCCA TGG^CCA
2250 TGGCCA
MroI 752 TCCGGA T^CCGGA
1739 TCCGGA
MscI 966 TGGCCA TGG^CCA
2250 TGGCCA
SmlI 1880 CTCAAG C^T[CT][GA]AG
2220 CTTGAG
SphI 630 GCATGC GCATG^C
1701 GCATGC
StuI 344 AGGCCT AGG^CCT
375 AGGCCT
XmnI 153 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
477 GAATGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI AvaII BanI BbsI BbvI
BclI BcnI BmyI BpmI BpuAI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
Eco81I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI Tru9I TseI Tsp45I
Tsp509I TspRI XcmI XhoII