################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AflIII AgeI AhdI ApaI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BbeI BbrPI BcgI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco52I EcoO65I EcoRI EcoT22I FseI FspI HindIII KasI KpnI MamI MfeI MluI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI TfiI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccII 12 CGCG CG^CG AcyI 960 GGCGTC G[GA]^CG[CT]C Aor51HI 612 AGCGCT AGC^GCT AvaI 1963 CCCGGG C^[CT]CG[GA]G BanII 1137 GGGCTC G[GA]GC[CT]^C BciVI 302 GTATCC GTATCC(6/5) BsaHI 960 GGCGTC G[GA]^CG[CT]C BsaI 1563 GGTCTC GGTCTC(1/5) BsgI 1132 GTGCAG GTGCAG(16/14) BsoBI 1963 CCCGGG C^[CT]CG[GA]G BsrBI 1415 GAGCGG CCGCTC(-3/-3) BsrFI 1274 ACCGGC [GA]^CCGG[CT] BstAPI 394 GCAGTTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 12 CGCG CG^CG Cfr10I 1274 ACCGGC [GA]^CCGG[CT] DrdI 859 GACTTCACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1968 GAAGAG CTCTTC(1/4) Eco47III 612 AGCGCT AGC^GCT EcoNI 1110 CCTGCCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG Hin1I 960 GGCGTC G[GA]^CG[CT]C HincII 1483 GTTAAC GT[CT]^[GA]AC HindII 1483 GTTAAC GT[CT]^[GA]AC HpaI 1483 GTTAAC GTT^AAC Ksp632I 1968 GAAGAG CTCTTC(1/4) KspI 11 CCGCGG CCGC^GG MaeII 114 ACGT A^CGT MvnI 12 CGCG CG^CG PflMI 696 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1724 GGGACCT [GA]G^G[AT]CC[CT] SacII 11 CCGCGG CCGC^GG SapI 1968 GAAGAGC GCTCTTC(1/4) SexAI 842 ACCAGGT A^CC[AT]GGT SmaI 1963 CCCGGG CCC^GGG TaqI 428 TCGA T^CGA TthHB8I 428 TCGA T^CGA Van91I 696 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 1963 CCCGGG C^CCGGG XmaI 1963 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccIII 752 TCCGGA T^CCGGA 1739 TCCGGA Alw44I 1029 GTGCAC G^TGCAC 1774 GTGCAC ApaLI 1029 GTGCAC G^TGCAC 1774 GTGCAC Asp700I 153 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 477 GAATGGCTTC AspHI 1029 GTGCAC G[AT]GC[AT]^C 1774 GTGCAC BalI 966 TGGCCA TGG^CCA 2250 TGGCCA BamHI 187 GGATCC G^GATCC 1736 GGATCC BbvCI 2103 CCTCAGC CCTCAGC(-5/-2) 448 GCTGAGG BfaI 763 CTAG C^TAG 1660 CTAG BsaWI 752 TCCGGA [AT]^CCGG[AT] 1739 TCCGGA BseAI 752 TCCGGA T^CCGGA 1739 TCCGGA BsiHKAI 1029 GTGCAC G[AT]GC[AT]^C 1774 GTGCAC BspEI 752 TCCGGA T^CCGGA 1739 TCCGGA BspMI 771 GCAGGT ACCTGC(4/8) 1422 GCAGGT DraII 1328 AGGCCCT [GA]G^G[ACGT]CC[CT] 1724 GGGACCT EcoO109I 1328 AGGCCCT [GA]G^G[ACGT]CC[CT] 1724 GGGACCT EcoRV 73 GATATC GAT^ATC 81 GATATC HaeII 612 AGCGCT [GA]GCGC^[CT] 1222 AGCGCC HgaI 961 GCGTC GACGC(5/10) 1358 GCGTC MaeI 763 CTAG C^TAG 1660 CTAG MluNI 966 TGGCCA TGG^CCA 2250 TGGCCA MroI 752 TCCGGA T^CCGGA 1739 TCCGGA MscI 966 TGGCCA TGG^CCA 2250 TGGCCA SmlI 1880 CTCAAG C^T[CT][GA]AG 2220 CTTGAG SphI 630 GCATGC GCATG^C 1701 GCATGC StuI 344 AGGCCT AGG^CCT 375 AGGCCT XmnI 153 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 477 GAATGGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 84 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AocI AvaII BanI BbsI BbvI BclI BcnI BmyI BpmI BpuAI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI Eco81I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI Tru9I TseI Tsp45I Tsp509I TspRI XcmI XhoII