##################################
# NO CUT ENZYMES ( 118 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Alw44I AocI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbrPI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BseAI BsgI BsiWI BsmBI BsmI
BspDI BspEI BspLU11I BspMI BsrBI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraIII DrdI
Eam1105I Eco81I EcoNI EcoO65I EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
MamI MfeI MflI MluI MluNI
MroI MscI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmaCI PmlI PshAI PshBI
Psp1406I PstI PvuI RsrII SalI
SbfI ScaI SexAI SfcI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I StyI SwaI Tth111I Van91I
XbaI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 395 GACGTC GACGT^C
AccI 603 GTAGAC GT^[AC][GT]AC
AflIII 1789 ACGTGT A^C[GA][CT]GT
AlwI 1367 GATCC GGATC(4/5)
ApaI 288 GGGCCC GGGCC^C
AspHI 693 GAGCTC G[AT]GC[AT]^C
BbeI 213 GGCGCC GGCGC^C
BbsI 1134 GAAGAC GAAGAC(2/6)
BcgI 1186 GCAAGTTTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1767 GCCATTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1134 GAAGAC GAAGAC(2/6)
BsaI 673 GGTCTC GGTCTC(1/5)
BsiHKAI 693 GAGCTC G[AT]GC[AT]^C
BsmAI 674 GTCTC GTCTC(1/5)
Bsp1407I 1783 TGTACA T^GTACA
BspHI 1678 TCATGA T^CATGA
BsrFI 246 ACCGGC [GA]^CCGG[CT]
BsrGI 1783 TGTACA T^GTACA
BssSI 304 CTCGTG CACGAG(-5/-1)
Cfr10I 246 ACCGGC [GA]^CCGG[CT]
DraII 32 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO109I 32 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 1059 GAATTC G^AATTC
HphI 1355 GGTGA GGTGA(8/7)
KasI 213 GGCGCC G^GCGCC
MslI 1263 CACTTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 213 GGCGCC GG^CGCC
NspI 1500 ACATGC [GA]CATG^[CT]
PleI 101 GACTC GAGTC(4/5)
PmeI 1595 GTTTAAAC GTTT^AAAC
PpuMI 32 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 1410 CAGCTG CAG^CTG
RcaI 1678 TCATGA T^CATGA
SacI 693 GAGCTC GAGCT^C
SapI 238 GAAGAGC GCTCTTC(1/4)
SfaNI 999 GCATC GCATC(5/9)
SfoI 213 GGCGCC GGC^GCC
SspBI 1783 TGTACA T^GTACA
StuI 358 AGGCCT AGG^CCT
XcmI 1768 CCATTGTGGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 213 GGCGCC G[GA]^CG[CT]C
395 GACGTC
AfaI 1428 GTAC GT^AC
1784 GTAC
Aor51HI 332 AGCGCT AGC^GCT
829 AGCGCT
BanI 213 GGCGCC G^G[CT][GA]CC
269 GGTGCC
BbvCI 568 CCTCAGC CCTCAGC(-5/-2)
335 GCTGAGG
BpmI 1727 CTCCAG CTGGAG(16/14)
1437 CTGGAG
BsaHI 213 GGCGCC G[GA]^CG[CT]C
395 GACGTC
BsaWI 195 TCCGGT [AT]^CCGG[AT]
387 ACCGGA
BsiEI 248 CGGCCG CG[GA][CT]^CG
409 CGGCCG
BsrDI 1073 CATTGC GCAATG(2/0)
958 GCAATG
BstF5I 1709 CATCC GGATG(2/0)
1550 GGATG
DraI 1157 TTTAAA TTT^AAA
1596 TTTAAA
EagI 248 CGGCCG C^GGCCG
409 CGGCCG
EclXI 248 CGGCCG C^GGCCG
409 CGGCCG
Eco47III 332 AGCGCT AGC^GCT
829 AGCGCT
Eco52I 248 CGGCCG C^GGCCG
409 CGGCCG
FokI 1709 CATCC GGATG(9/13)
1550 GGATG
HgaI 157 GCGTC GACGC(5/10)
458 GCGTC
Hin1I 213 GGCGCC G[GA]^CG[CT]C
395 GACGTC
PaeR7I 993 CTCGAG C^TCGAG
1141 CTCGAG
RsaI 1428 GTAC GT^AC
1784 GTAC
SmaI 421 CCCGGG CCC^GGG
462 CCCGGG
SspI 1230 AATATT AAT^ATT
1292 AATATT
TaqI 994 TCGA T^CGA
1142 TCGA
TfiI 534 GATTC G^A[AT]TC
1760 GAATC
TthHB8I 994 TCGA T^CGA
1142 TCGA
XhoI 993 CTCGAG C^TCGAG
1141 CTCGAG
XmaCI 421 CCCGGG C^CCGGG
462 CCCGGG
XmaI 421 CCCGGG C^CCGGG
462 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AcsI AluI AlwNI
ApoI AvaI AvaII BanII BbvI
BcnI BfaI BmyI BsaJI BseRI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrI BssKI BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI EarI EcoRII FauI Fnu4HI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV SacII Sau3AI Sau96I ScrFI
SmlI Tru9I TseI Tsp45I Tsp509I
TspRI