##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
AspEI AspI AviII AvrII BalI
BbeI BbsI BbvCI BcgI BclI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BseAI BsiEI BsiWI
BsmI BspDI BspEI BspHI BspLU11I
BspMI BssSI Bst1107I BstAPI BstBI
BstZ17I CelII ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoRI EcoRV EcoT22I
FbaI FseI FspI HpaI KasI
MamI MfeI MluI MluNI MroI
MscI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PflFI PflMI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspI SwaI Tth111I Van91I XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 2246 GGTACC G^GTACC
AccI 1714 GTCTAC GT^[AC][GT]AC
AcyI 1922 GACGCC G[GA]^CG[CT]C
AocI 1585 CCTGAGG CC^T[ACGT]AGG
Asp718I 2246 GGTACC G^GTACC
BbrPI 825 CACGTG CAC^GTG
BciVI 2046 GGATAC GTATCC(6/5)
BglI 1071 GCCTAGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 825 CACGTG [CT]AC^GT[GA]
BsaHI 1922 GACGCC G[GA]^CG[CT]C
BsaWI 1119 ACCGGA [AT]^CCGG[AT]
Bsp1407I 2439 TGTACA T^GTACA
BsrBI 1369 GAGCGG CCGCTC(-3/-3)
BsrDI 1060 CATTGC GCAATG(2/0)
BsrGI 2439 TGTACA T^GTACA
BssHII 321 GCGCGC G^CGCGC
BstEII 652 GGTCACC G^GT[ACGT]ACC
BstPI 652 GGTCACC G^GT[ACGT]ACC
BstXI 2103 CCACGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1585 CCTGAGG CC^T[ACGT]AGG
DrdI 104 GACCTGGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1650 TGGCCG [CT]^GGCC[GA]
Eco81I 1585 CCTGAGG CC^T[ACGT]AGG
EcoO65I 652 GGTCACC G^GT[ACGT]ACC
Hin1I 1922 GACGCC G[GA]^CG[CT]C
HindIII 1423 AAGCTT A^AGCTT
KpnI 2246 GGTACC GGTAC^C
KspI 54 CCGCGG CCGC^GG
NspI 759 GCATGC [GA]CATG^[CT]
PaeR7I 153 CTCGAG C^TCGAG
PmaCI 825 CACGTG CAC^GTG
PmlI 825 CACGTG CAC^GTG
PshAI 1809 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 54 CCGCGG CCGC^GG
SapI 2135 GAAGAGC GCTCTTC(1/4)
SexAI 799 ACCTGGT A^CC[AT]GGT
SfaNI 1447 GCATC GCATC(5/9)
SfiI 1070 GGCCTAGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 759 GCATGC GCATG^C
SspBI 2439 TGTACA T^GTACA
XcmI 2121 CCACCGTGACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 153 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AflIII 486 ACGTGT A^C[GA][CT]GT
826 ACGTGT
BamHI 246 GGATCC G^GATCC
1581 GGATCC
BsgI 1337 CTGCAC GTGCAG(16/14)
677 GTGCAG
BsmBI 2232 CGTCTC CGTCTC(1/5)
292 GAGACG
BstYI 246 GGATCC [GA]^GATC[CT]
1581 GGATCC
DsaI 54 CCGCGG C^C[GA][CT]GG
2103 CCACGG
HaeII 301 GGCGCT [GA]GCGC^[CT]
328 GGCGCT
MflI 246 GGATCC [GA]^GATC[CT]
1581 GGATCC
MslI 949 CACAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2078 CATGCCTGTG
NaeI 130 GCCGGC GCC^GGC
214 GCCGGC
NgoMIV 130 GCCGGC G^CCGGC
214 GCCGGC
PpuMI 102 GGGACCT [GA]G^G[AT]CC[CT]
1538 GGGACCC
PstI 19 CTGCAG CTGCA^G
338 CTGCAG
SmlI 153 CTCGAG C^T[CT][GA]AG
2293 CTTGAG
StuI 1069 AGGCCT AGG^CCT
1674 AGGCCT
Tsp509I 358 AATT ^AATT
1753 AATT
XhoII 246 GGATCC [GA]^GATC[CT]
1581 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI Asp700I AspHI AvaI AvaII
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I TspRI
TthHB8I XmnI