################################## # NO CUT ENZYMES ( 107 enzymes) # ################################## AatII AccIII AclI AcsI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI AspEI AspI AviII AvrII BalI BbeI BbsI BbvCI BcgI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BseAI BsiEI BsiWI BsmI BspDI BspEI BspHI BspLU11I BspMI BssSI Bst1107I BstAPI BstBI BstZ17I CelII ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HpaI KasI MamI MfeI MluI MluNI MroI MscI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI Tth111I Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### Acc65I 2246 GGTACC G^GTACC AccI 1714 GTCTAC GT^[AC][GT]AC AcyI 1922 GACGCC G[GA]^CG[CT]C AocI 1585 CCTGAGG CC^T[ACGT]AGG Asp718I 2246 GGTACC G^GTACC BbrPI 825 CACGTG CAC^GTG BciVI 2046 GGATAC GTATCC(6/5) BglI 1071 GCCTAGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 825 CACGTG [CT]AC^GT[GA] BsaHI 1922 GACGCC G[GA]^CG[CT]C BsaWI 1119 ACCGGA [AT]^CCGG[AT] Bsp1407I 2439 TGTACA T^GTACA BsrBI 1369 GAGCGG CCGCTC(-3/-3) BsrDI 1060 CATTGC GCAATG(2/0) BsrGI 2439 TGTACA T^GTACA BssHII 321 GCGCGC G^CGCGC BstEII 652 GGTCACC G^GT[ACGT]ACC BstPI 652 GGTCACC G^GT[ACGT]ACC BstXI 2103 CCACGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1585 CCTGAGG CC^T[ACGT]AGG DrdI 104 GACCTGGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 1650 TGGCCG [CT]^GGCC[GA] Eco81I 1585 CCTGAGG CC^T[ACGT]AGG EcoO65I 652 GGTCACC G^GT[ACGT]ACC Hin1I 1922 GACGCC G[GA]^CG[CT]C HindIII 1423 AAGCTT A^AGCTT KpnI 2246 GGTACC GGTAC^C KspI 54 CCGCGG CCGC^GG NspI 759 GCATGC [GA]CATG^[CT] PaeR7I 153 CTCGAG C^TCGAG PmaCI 825 CACGTG CAC^GTG PmlI 825 CACGTG CAC^GTG PshAI 1809 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 54 CCGCGG CCGC^GG SapI 2135 GAAGAGC GCTCTTC(1/4) SexAI 799 ACCTGGT A^CC[AT]GGT SfaNI 1447 GCATC GCATC(5/9) SfiI 1070 GGCCTAGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SphI 759 GCATGC GCATG^C SspBI 2439 TGTACA T^GTACA XcmI 2121 CCACCGTGACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 153 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AflIII 486 ACGTGT A^C[GA][CT]GT 826 ACGTGT BamHI 246 GGATCC G^GATCC 1581 GGATCC BsgI 1337 CTGCAC GTGCAG(16/14) 677 GTGCAG BsmBI 2232 CGTCTC CGTCTC(1/5) 292 GAGACG BstYI 246 GGATCC [GA]^GATC[CT] 1581 GGATCC DsaI 54 CCGCGG C^C[GA][CT]GG 2103 CCACGG HaeII 301 GGCGCT [GA]GCGC^[CT] 328 GGCGCT MflI 246 GGATCC [GA]^GATC[CT] 1581 GGATCC MslI 949 CACAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2078 CATGCCTGTG NaeI 130 GCCGGC GCC^GGC 214 GCCGGC NgoMIV 130 GCCGGC G^CCGGC 214 GCCGGC PpuMI 102 GGGACCT [GA]G^G[AT]CC[CT] 1538 GGGACCC PstI 19 CTGCAG CTGCA^G 338 CTGCAG SmlI 153 CTCGAG C^T[CT][GA]AG 2293 CTTGAG StuI 1069 AGGCCT AGG^CCT 1674 AGGCCT Tsp509I 358 AATT ^AATT 1753 AATT XhoII 246 GGATCC [GA]^GATC[CT] 1581 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 92 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI Asp700I AspHI AvaI AvaII BanI BanII BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I TspRI TthHB8I XmnI