##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
Acc65I AccIII AclI AflII AhdI
AocI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbvCI BclI BfrI BlnI
BsaI BseAI BsiEI BsiWI BsoBI
BspEI BspMI BsrBI BssSI Bst1107I
BstBI BstZ17I Bsu36I CpoI DraII
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoRV EcoT14I EcoT22I FbaI
FseI FspI HaeII HpaI KasI
KpnI KspI MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PmeI PpuMI PshBI Psp1406I
PvuI RsrII SacII SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
StyI SwaI TfiI Tth111I XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 1308 GACGTC GACGT^C
AcyI 1308 GACGTC G[GA]^CG[CT]C
AgeI 132 ACCGGT A^CCGGT
Alw44I 301 GTGCAC G^TGCAC
ApaLI 301 GTGCAC G^TGCAC
BbrPI 1857 CACGTG CAC^GTG
BcgI 406 GCAGTCGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 16 CCCGG CC^[GC]GG
BglI 1145 GCCCAGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 702 AGATCT A^GATCT
BlpI 1117 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1117 GCTGAGC GC^T[ACGT]AGC
BsaAI 1857 CACGTG [CT]AC^GT[GA]
BsaBI 2220 GATAGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1308 GACGTC G[GA]^CG[CT]C
BsaWI 132 ACCGGT [AT]^CCGG[AT]
BsmBI 1306 GAGACG CGTCTC(1/5)
Bsp1407I 1992 TGTACA T^GTACA
BspDI 889 ATCGAT AT^CGAT
BspHI 308 TCATGA T^CATGA
BsrFI 132 ACCGGT [GA]^CCGG[CT]
BsrGI 1992 TGTACA T^GTACA
BssHII 78 GCGCGC G^CGCGC
BstEII 2209 GGTGACC G^GT[ACGT]ACC
BstPI 2209 GGTGACC G^GT[ACGT]ACC
BstXI 356 CCAGTGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1117 GCTGAGC GC^T[ACGT]AGC
Cfr10I 132 ACCGGT [GA]^CCGG[CT]
ClaI 889 ATCGAT AT^CGAT
DraI 2482 TTTAAA TTT^AAA
DsaI 1675 CCACGG C^C[GA][CT]GG
EcoO65I 2209 GGTGACC G^GT[ACGT]ACC
EcoRI 2005 GAATTC G^AATTC
HgaI 54 GACGC GACGC(5/10)
Hin1I 1308 GACGTC G[GA]^CG[CT]C
HincII 956 GTCGAC GT[CT]^[GA]AC
HindII 956 GTCGAC GT[CT]^[GA]AC
HindIII 927 AAGCTT A^AGCTT
MamI 2220 GATAGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 1206 CATCAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 16 CCCGG CC^[GC]GG
PflMI 1074 CCAGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 132 ACCGGT A^CCGGT
PmaCI 1857 CACGTG CAC^GTG
PmlI 1857 CACGTG CAC^GTG
PshAI 1846 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1605 CTGCAG CTGCA^G
RcaI 308 TCATGA T^CATGA
SacI 321 GAGCTC GAGCT^C
SalI 956 GTCGAC G^TCGAC
SspBI 1992 TGTACA T^GTACA
Van91I 1074 CCAGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 705 TCTAGA T^CTAGA
XcmI 1205 CCATCAGTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwNI 1075 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
1250 CAGGGACTG
BanII 321 GAGCTC G[GA]GC[CT]^C
1923 GGGCTC
BbsI 466 GAAGAC GAAGAC(2/6)
1166 GAAGAC
BpmI 1081 CTGGAG CTGGAG(16/14)
1216 CTGGAG
BpuAI 466 GAAGAC GAAGAC(2/6)
1166 GAAGAC
BsgI 1598 CTGCAC GTGCAG(16/14)
2085 GTGCAG
BspLU11I 1483 ACATGT A^CATGT
2081 ACATGT
BsrDI 326 CATTGC GCAATG(2/0)
1458 CATTGC
BstAPI 924 GCAAAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1935 GCACTTACTGC
BstYI 702 AGATCT [GA]^GATC[CT]
1986 AGATCC
HaeIII 103 GGCC GG^CC
1153 GGCC
HapII 17 CCGG C^CGG
133 CCGG
HpaII 17 CCGG C^CGG
133 CCGG
MaeII 1309 ACGT A^CGT
1858 ACGT
MflI 702 AGATCT [GA]^GATC[CT]
1986 AGATCC
MspI 17 CCGG C^CGG
133 CCGG
NspI 1483 ACATGT [GA]CATG^[CT]
2081 ACATGT
PvuII 2372 CAGCTG CAG^CTG
2512 CAGCTG
SapI 197 GAAGAGC GCTCTTC(1/4)
948 GCTCTTC
ScaI 2398 AGTACT AGT^ACT
2453 AGTACT
SfcI 1605 CTGCAG C^T[GA][CT]AG
1908 CTGTAG
SmlI 184 CTCAAG C^T[CT][GA]AG
2394 CTTGAG
SspI 765 AATATT AAT^ATT
1366 AATATT
XhoII 702 AGATCT [GA]^GATC[CT]
1986 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI ApoI AspHI
AvaII BanI BbvI BciVI BfaI
BmyI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
EarI EcoRII FauI Fnu4HI FokI
HhaI HinP1I HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MseI MspA1I MvaI MvnI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I