##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI Aor51HI ApaI AscI
Asp718I AspEI AviII AvrII BanII
BcgI BciVI BclI BfrI BlnI
BlpI Bpu1102I BsaBI BsaI BsiEI
BsiWI BsmBI Bsp1407I BsrBI BsrGI
BssHII Bst1107I BstAPI BstBI BstZ17I
CelII CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoRI EcoRV EcoT22I FbaI FseI
FspI HpaI KpnI KspI MamI
MfeI MluI MunI NaeI NdeI
NgoMIV NheI NotI NsiI NspV
PacI PflMI PinAI PmeI PpuMI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI ScaI SfiI SfuI
SnaBI SpeI SphI SspBI SspI
SwaI Van91I XbaI XcmI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AlwNI 198 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1858 CCTCAGG CC^T[ACGT]AGG
AseI 2035 ATTAAT AT^TAAT
AsnI 2035 ATTAAT AT^TAAT
Asp700I 1582 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 757 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1005 GGATCC G^GATCC
BbeI 474 GGCGCC GGCGC^C
BbvCI 503 GCTGAGG CCTCAGC(-5/-2)
BfaI 2051 CTAG C^TAG
BglII 972 AGATCT A^GATCT
BsmI 1648 GAATGC GAATGC(1/-1)
BspHI 174 TCATGA T^CATGA
BspLU11I 486 ACATGT A^CATGT
BspMI 82 GCAGGT ACCTGC(4/8)
BsrDI 1086 GCAATG GCAATG(2/0)
BsrFI 145 GCCGGT [GA]^CCGG[CT]
BssSI 1349 CACGAG CACGAG(-5/-1)
BstEII 1869 GGTCACC G^GT[ACGT]ACC
BstPI 1869 GGTCACC G^GT[ACGT]ACC
BstXI 1272 CCATGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1858 CCTCAGG CC^T[ACGT]AGG
Cfr10I 145 GCCGGT [GA]^CCGG[CT]
DraII 900 AGGCCCT [GA]G^G[ACGT]CC[CT]
Eco81I 1858 CCTCAGG CC^T[ACGT]AGG
EcoO109I 900 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1869 GGTCACC G^GT[ACGT]ACC
HindIII 1602 AAGCTT A^AGCTT
KasI 474 GGCGCC G^GCGCC
MaeI 2051 CTAG C^TAG
NarI 474 GGCGCC GG^CGCC
NruI 319 TCGCGA TCG^CGA
NspI 486 ACATGT [GA]CATG^[CT]
PaeR7I 1401 CTCGAG C^TCGAG
PflFI 757 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 2035 ATTAAT AT^TAAT
RcaI 174 TCATGA T^CATGA
SapI 532 GAAGAGC GCTCTTC(1/4)
SbfI 1216 CCTGCAGG CCTGCA^GG
SfoI 474 GGCGCC GGC^GCC
SgrAI 144 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 1032 CCCGGG CCC^GGG
SmlI 1401 CTCGAG C^T[CT][GA]AG
Sse8387I 1216 CCTGCAGG CCTGCA^GG
Tth111I 757 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1401 CTCGAG C^TCGAG
XmaCI 1032 CCCGGG C^CCGGG
XmaI 1032 CCCGGG C^CCGGG
XmnI 1582 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccIII 492 TCCGGA T^CCGGA
696 TCCGGA
AcyI 142 GACGCC G[GA]^CG[CT]C
474 GGCGCC
AflIII 486 ACATGT A^C[GA][CT]GT
872 ACGTGT
Alw44I 111 GTGCAC G^TGCAC
1015 GTGCAC
ApaLI 111 GTGCAC G^TGCAC
1015 GTGCAC
AvaII 514 GGACC G^G[AT]CC
820 GGTCC
BbrPI 53 CACGTG CAC^GTG
871 CACGTG
BsaAI 53 CACGTG [CT]AC^GT[GA]
871 CACGTG
BsaHI 142 GACGCC G[GA]^CG[CT]C
474 GGCGCC
BsaWI 492 TCCGGA [AT]^CCGG[AT]
696 TCCGGA
BseAI 492 TCCGGA T^CCGGA
696 TCCGGA
BseRI 269 GAGGAG GAGGAG(10/8)
1172 GAGGAG
BsmAI 1963 GAGAC GTCTC(1/5)
1823 GTCTC
BspDI 1107 ATCGAT AT^CGAT
1481 ATCGAT
BspEI 492 TCCGGA T^CCGGA
696 TCCGGA
ClaI 1107 ATCGAT AT^CGAT
1481 ATCGAT
DraI 1502 TTTAAA TTT^AAA
1736 TTTAAA
HaeII 474 GGCGCC [GA]GCGC^[CT]
910 GGCGCT
Hin1I 142 GACGCC G[GA]^CG[CT]C
474 GGCGCC
HincII 1780 GTTGAC GT[CT]^[GA]AC
1845 GTTGAC
HindII 1780 GTTGAC GT[CT]^[GA]AC
1845 GTTGAC
MroI 492 TCCGGA T^CCGGA
696 TCCGGA
PleI 316 GACTC GAGTC(4/5)
74 GAGTC
PmaCI 53 CACGTG CAC^GTG
871 CACGTG
PmlI 53 CACGTG CAC^GTG
871 CACGTG
SexAI 288 ACCAGGT A^CC[AT]GGT
855 ACCTGGT
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaI BalI
BanI BbsI BbvI BcnI BglI
BmyI BpmI BpuAI BsaJI BsgI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EaeI EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII