################################## # NO CUT ENZYMES ( 89 enzymes) # ################################## AatII Acc65I AccI AclI AflII AgeI AhdI Aor51HI ApaI AscI Asp718I AspEI AviII AvrII BanII BcgI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaBI BsaI BsiEI BsiWI BsmBI Bsp1407I BsrBI BsrGI BssHII Bst1107I BstAPI BstBI BstZ17I CelII CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HpaI KpnI KspI MamI MfeI MluI MunI NaeI NdeI NgoMIV NheI NotI NsiI NspV PacI PflMI PinAI PmeI PpuMI PshAI Psp1406I PvuI RsrII SacI SacII SalI ScaI SfiI SfuI SnaBI SpeI SphI SspBI SspI SwaI Van91I XbaI XcmI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AlwNI 198 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1858 CCTCAGG CC^T[ACGT]AGG AseI 2035 ATTAAT AT^TAAT AsnI 2035 ATTAAT AT^TAAT Asp700I 1582 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 757 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 1005 GGATCC G^GATCC BbeI 474 GGCGCC GGCGC^C BbvCI 503 GCTGAGG CCTCAGC(-5/-2) BfaI 2051 CTAG C^TAG BglII 972 AGATCT A^GATCT BsmI 1648 GAATGC GAATGC(1/-1) BspHI 174 TCATGA T^CATGA BspLU11I 486 ACATGT A^CATGT BspMI 82 GCAGGT ACCTGC(4/8) BsrDI 1086 GCAATG GCAATG(2/0) BsrFI 145 GCCGGT [GA]^CCGG[CT] BssSI 1349 CACGAG CACGAG(-5/-1) BstEII 1869 GGTCACC G^GT[ACGT]ACC BstPI 1869 GGTCACC G^GT[ACGT]ACC BstXI 1272 CCATGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1858 CCTCAGG CC^T[ACGT]AGG Cfr10I 145 GCCGGT [GA]^CCGG[CT] DraII 900 AGGCCCT [GA]G^G[ACGT]CC[CT] Eco81I 1858 CCTCAGG CC^T[ACGT]AGG EcoO109I 900 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoO65I 1869 GGTCACC G^GT[ACGT]ACC HindIII 1602 AAGCTT A^AGCTT KasI 474 GGCGCC G^GCGCC MaeI 2051 CTAG C^TAG NarI 474 GGCGCC GG^CGCC NruI 319 TCGCGA TCG^CGA NspI 486 ACATGT [GA]CATG^[CT] PaeR7I 1401 CTCGAG C^TCGAG PflFI 757 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 2035 ATTAAT AT^TAAT RcaI 174 TCATGA T^CATGA SapI 532 GAAGAGC GCTCTTC(1/4) SbfI 1216 CCTGCAGG CCTGCA^GG SfoI 474 GGCGCC GGC^GCC SgrAI 144 CGCCGGTG C[GA]^CCGG[CT]G SmaI 1032 CCCGGG CCC^GGG SmlI 1401 CTCGAG C^T[CT][GA]AG Sse8387I 1216 CCTGCAGG CCTGCA^GG Tth111I 757 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 1401 CTCGAG C^TCGAG XmaCI 1032 CCCGGG C^CCGGG XmaI 1032 CCCGGG C^CCGGG XmnI 1582 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccIII 492 TCCGGA T^CCGGA 696 TCCGGA AcyI 142 GACGCC G[GA]^CG[CT]C 474 GGCGCC AflIII 486 ACATGT A^C[GA][CT]GT 872 ACGTGT Alw44I 111 GTGCAC G^TGCAC 1015 GTGCAC ApaLI 111 GTGCAC G^TGCAC 1015 GTGCAC AvaII 514 GGACC G^G[AT]CC 820 GGTCC BbrPI 53 CACGTG CAC^GTG 871 CACGTG BsaAI 53 CACGTG [CT]AC^GT[GA] 871 CACGTG BsaHI 142 GACGCC G[GA]^CG[CT]C 474 GGCGCC BsaWI 492 TCCGGA [AT]^CCGG[AT] 696 TCCGGA BseAI 492 TCCGGA T^CCGGA 696 TCCGGA BseRI 269 GAGGAG GAGGAG(10/8) 1172 GAGGAG BsmAI 1963 GAGAC GTCTC(1/5) 1823 GTCTC BspDI 1107 ATCGAT AT^CGAT 1481 ATCGAT BspEI 492 TCCGGA T^CCGGA 696 TCCGGA ClaI 1107 ATCGAT AT^CGAT 1481 ATCGAT DraI 1502 TTTAAA TTT^AAA 1736 TTTAAA HaeII 474 GGCGCC [GA]GCGC^[CT] 910 GGCGCT Hin1I 142 GACGCC G[GA]^CG[CT]C 474 GGCGCC HincII 1780 GTTGAC GT[CT]^[GA]AC 1845 GTTGAC HindII 1780 GTTGAC GT[CT]^[GA]AC 1845 GTTGAC MroI 492 TCCGGA T^CCGGA 696 TCCGGA PleI 316 GACTC GAGTC(4/5) 74 GAGTC PmaCI 53 CACGTG CAC^GTG 871 CACGTG PmlI 53 CACGTG CAC^GTG 871 CACGTG SexAI 288 ACCAGGT A^CC[AT]GGT 855 ACCTGGT ######################################### # 3 OR MORE CUT ENZYMES ( 94 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI ApoI AspHI AvaI BalI BanI BbsI BbvI BcnI BglI BmyI BpmI BpuAI BsaJI BsgI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII