##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BbeI BbvCI BciVI BclI
BfrI BglII BlnI BsaWI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrGI BssHII Bst1107I
BstAPI BstBI BstXI BstZ17I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
KspI MfeI MluI MroI MunI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI XbaI
XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccII 26 CGCG CG^CG
AcyI 1364 GACGCC G[GA]^CG[CT]C
AocI 87 CCTTAGG CC^T[ACGT]AGG
ApaI 408 GGGCCC GGGCC^C
Asp700I 283 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1352 CTCGAG C^[CT]CG[GA]G
AvaII 1165 GGACC G^G[AT]CC
BalI 1633 TGGCCA TGG^CCA
BamHI 804 GGATCC G^GATCC
BbrPI 173 CACGTG CAC^GTG
BbsI 669 GTCTTC GAAGAC(2/6)
BfaI 1086 CTAG C^TAG
BlpI 463 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 463 GCTCAGC GC^T[ACGT]AGC
BpuAI 669 GTCTTC GAAGAC(2/6)
BsaHI 1364 GACGCC G[GA]^CG[CT]C
BsoBI 1352 CTCGAG C^[CT]CG[GA]G
BspMI 762 ACCTGC ACCTGC(4/8)
BstF5I 1523 GGATG GGATG(2/0)
BstUI 26 CGCG CG^CG
Bsu36I 87 CCTTAGG CC^T[ACGT]AGG
CelII 463 GCTCAGC GC^T[ACGT]AGC
DrdI 728 GACGACGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1633 TGGCCA [CT]^GGCC[GA]
Eco81I 87 CCTTAGG CC^T[ACGT]AGG
EcoNI 1774 CCTCGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 1523 GGATG GGATG(9/13)
HaeII 56 GGCGCT [GA]GCGC^[CT]
Hin1I 1364 GACGCC G[GA]^CG[CT]C
MaeI 1086 CTAG C^TAG
MluNI 1633 TGGCCA TGG^CCA
MscI 1633 TGGCCA TGG^CCA
MvnI 26 CGCG CG^CG
NaeI 1287 GCCGGC GCC^GGC
NgoMIV 1287 GCCGGC G^CCGGC
PaeR7I 1352 CTCGAG C^TCGAG
PmaCI 173 CACGTG CAC^GTG
PmlI 173 CACGTG CAC^GTG
SexAI 66 ACCTGGT A^CC[AT]GGT
SgrAI 1286 CGCCGGCG C[GA]^CCGG[CT]G
XhoI 1352 CTCGAG C^TCGAG
XmnI 283 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AlwNI 212 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1857 CAGGGCCTG
AspI 731 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
778 GACGGTGTC
BanI 430 GGTGCC G^G[CT][GA]CC
1301 GGCACC
BcgI 1046 CGAATGCGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
346 GCAAGCTGTTCG
BglI 152 GCCTACGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
923 GCCTGTGTGGC
BsaAI 155 TACGTG [CT]AC^GT[GA]
173 CACGTG
BsaBI 1210 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1496 GATGAGGATC
BsaI 419 GAGACC GGTCTC(1/5)
856 GGTCTC
BsgI 188 CTGCAC GTGCAG(16/14)
224 CTGCAC
BsmBI 22 GAGACG CGTCTC(1/5)
1309 GAGACG
BsmI 1047 GAATGC GAATGC(1/-1)
269 GCATTC
BspHI 1155 TCATGA T^CATGA
1206 TCATGA
BssSI 227 CACGAG CACGAG(-5/-1)
1096 CACGAG
DraII 1639 GGGGCCT [GA]G^G[ACGT]CC[CT]
1858 AGGGCCT
EarI 581 CTCTTC CTCTTC(1/4)
1596 CTCTTC
EcoO109I 1639 GGGGCCT [GA]G^G[ACGT]CC[CT]
1858 AGGGCCT
HgaI 24 GACGC GACGC(5/10)
1364 GACGC
Ksp632I 581 CTCTTC CTCTTC(1/4)
1596 CTCTTC
MamI 1210 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1496 GATGAGGATC
MseI 390 TTAA T^TAA
1915 TTAA
MslI 169 CATTCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
173 CACGTGAATG
NspI 96 ACATGC [GA]CATG^[CT]
342 ACATGC
PflFI 731 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
778 GACGGTGTC
PflMI 211 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
365 CCATTGCTTGG
PvuII 995 CAGCTG CAG^CTG
1514 CAGCTG
RcaI 1155 TCATGA T^CATGA
1206 TCATGA
SacI 79 GAGCTC GAGCT^C
983 GAGCTC
SmlI 1352 CTCGAG C^T[CT][GA]AG
1393 CTCAAG
Tru9I 390 TTAA T^TAA
1915 TTAA
Tsp509I 1472 AATT ^AATT
1725 AATT
Tth111I 731 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
778 GACGGTGTC
Van91I 211 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
365 CCATTGCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
BanII BbvI BcnI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrDI BsrFI
BsrI BssKI BstEII BstNI BstPI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DsaI EcoO65I EcoRII
EcoT14I FauI Fnu4HI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TseI Tsp45I TspRI TthHB8I
XhoII