##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BbeI BbrPI BciVI BlnI
BsaAI BsaWI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BsrBI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco52I EcoRV FseI FspI HincII
HindII HpaI KasI KpnI KspI
MfeI MluI MroI MunI NarI
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI SwaI XbaI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 1201 GTCTAC GT^[AC][GT]AC
AccII 11 CGCG CG^CG
AcyI 860 GACGCC G[GA]^CG[CT]C
AflII 2364 CTTAAG C^TTAAG
AocI 1492 CCTGAGG CC^T[ACGT]AGG
ApaI 830 GGGCCC GGGCC^C
AspI 1219 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 2033 GCAGTGTTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 90 TGATCA T^GATCA
BfrI 2364 CTTAAG C^TTAAG
BglII 2130 AGATCT A^GATCT
BlpI 2216 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2216 GCTAAGC GC^T[ACGT]AGC
BsaBI 1084 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 860 GACGCC G[GA]^CG[CT]C
BsgI 1723 GTGCAG GTGCAG(16/14)
BsmI 846 GAATGC GAATGC(1/-1)
BspHI 671 TCATGA T^CATGA
BstEII 1596 GGTGACC G^GT[ACGT]ACC
BstPI 1596 GGTGACC G^GT[ACGT]ACC
BstUI 11 CGCG CG^CG
Bsu36I 1492 CCTGAGG CC^T[ACGT]AGG
CelII 2216 GCTAAGC GC^T[ACGT]AGC
DraII 1193 AGGCCCT [GA]G^G[ACGT]CC[CT]
Eco81I 1492 CCTGAGG CC^T[ACGT]AGG
EcoNI 2243 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1193 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1596 GGTGACC G^GT[ACGT]ACC
EcoRI 415 GAATTC G^AATTC
EcoT22I 871 ATGCAT ATGCA^T
FbaI 90 TGATCA T^GATCA
HgaI 860 GACGC GACGC(5/10)
Hin1I 860 GACGCC G[GA]^CG[CT]C
HindIII 994 AAGCTT A^AGCTT
MamI 1084 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 11 CGCG CG^CG
NaeI 6 GCCGGC GCC^GGC
NdeI 1380 CATATG CA^TATG
NgoMIV 6 GCCGGC G^CCGGC
NheI 1260 GCTAGC G^CTAGC
NsiI 871 ATGCAT ATGCA^T
PaeR7I 198 CTCGAG C^TCGAG
PflFI 1219 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 136 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 671 TCATGA T^CATGA
SacI 481 GAGCTC GAGCT^C
SapI 1540 GAAGAGC GCTCTTC(1/4)
SexAI 1670 ACCAGGT A^CC[AT]GGT
Tth111I 1219 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1246 CCAGGGGGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 198 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 415 GAATTC [GA]^AATT[CT]
1015 AAATTC
Aor51HI 956 AGCGCT AGC^GCT
1073 AGCGCT
ApoI 415 GAATTC [GA]^AATT[CT]
1015 AAATTC
AvaII 1128 GGACC G^G[AT]CC
1440 GGACC
BalI 903 TGGCCA TGG^CCA
1304 TGGCCA
BamHI 229 GGATCC G^GATCC
840 GGATCC
BbvCI 123 CCTCAGC CCTCAGC(-5/-2)
1953 GCTGAGG
BpmI 138 CTCCAG CTGGAG(16/14)
2317 CTCCAG
BsrDI 2084 CATTGC GCAATG(2/0)
389 GCAATG
BssSI 473 CACGAG CACGAG(-5/-1)
1762 CTCGTG
BstAPI 283 GCACAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1656 GCAAGTGCTGC
DraIII 679 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
1669 CACCAGGTG
Eco47III 956 AGCGCT AGC^GCT
1073 AGCGCT
EcoT14I 1400 CCATGG C^C[AT][AT]GG
2049 CCATGG
HaeII 956 AGCGCT [GA]GCGC^[CT]
1073 AGCGCT
MluNI 903 TGGCCA TGG^CCA
1304 TGGCCA
MscI 903 TGGCCA TGG^CCA
1304 TGGCCA
MseI 998 TTAA T^TAA
2365 TTAA
NcoI 1400 CCATGG C^CATGG
2049 CCATGG
StuI 174 AGGCCT AGG^CCT
1970 AGGCCT
StyI 1400 CCATGG C^C[AT][AT]GG
2049 CCATGG
Tru9I 998 TTAA T^TAA
2365 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI AspHI AvaI BanI BanII
BbsI BbvI BcnI BfaI BglI
BmyI BpuAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspLU11I BspMI
BsrFI BsrGI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DsaI
EaeI EarI EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII