##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AflIII AgeI AhdI Alw44I AocI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII BbsI BcgI
BciVI BfrI BpuAI BsgI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspLU11I
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
FseI FspI HindIII HpaI KpnI
KspI MfeI MluI MseI MunI
MvnI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI PshBI Psp1406I PvuI RsrII
SacI SacII SbfI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tth111I Van91I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 1326 GTCGAC GT^[AC][GT]AC
AccIII 1138 TCCGGA T^CCGGA
AlwNI 426 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1624 AGCGCT AGC^GCT
ApaI 127 GGGCCC GGGCC^C
AspHI 1940 GAGCAC G[AT]GC[AT]^C
AvrII 1382 CCTAGG C^CTAGG
BamHI 449 GGATCC G^GATCC
BanII 127 GGGCCC G[GA]GC[CT]^C
BbrPI 471 CACGTG CAC^GTG
BbvCI 369 GCTGAGG CCTCAGC(-5/-2)
BclI 1562 TGATCA T^GATCA
BglI 573 GCCAAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2002 AGATCT A^GATCT
BlnI 1382 CCTAGG C^CTAGG
BlpI 758 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 758 GCTGAGC GC^T[ACGT]AGC
BsaAI 471 CACGTG [CT]AC^GT[GA]
BsaBI 983 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1614 GGTCTC GGTCTC(1/5)
BsaWI 1138 TCCGGA [AT]^CCGG[AT]
BseAI 1138 TCCGGA T^CCGGA
BsiHKAI 1940 GAGCAC G[AT]GC[AT]^C
BsmI 1470 GAATGC GAATGC(1/-1)
BspEI 1138 TCCGGA T^CCGGA
BsrDI 1660 GCAATG GCAATG(2/0)
BsrFI 1734 GCCGGT [GA]^CCGG[CT]
BstAPI 803 GCACATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1292 CCACCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 758 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1734 GCCGGT [GA]^CCGG[CT]
Eco47III 1624 AGCGCT AGC^GCT
EcoT22I 1538 ATGCAT ATGCA^T
FauI 1806 GCGGG CCCGC(4/6)
FbaI 1562 TGATCA T^GATCA
HgaI 251 GCGTC GACGC(5/10)
HincII 1326 GTCGAC GT[CT]^[GA]AC
HindII 1326 GTCGAC GT[CT]^[GA]AC
MaeII 472 ACGT A^CGT
MamI 983 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1138 TCCGGA T^CCGGA
NsiI 1538 ATGCAT ATGCA^T
PmaCI 471 CACGTG CAC^GTG
PmlI 471 CACGTG CAC^GTG
PshAI 536 GACTGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 1326 GTCGAC G^TCGAC
ScaI 1871 AGTACT AGT^ACT
SmlI 1371 CTTGAG C^T[CT][GA]AG
XbaI 1963 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Asp700I 675 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1869 GAAGTACTTC
AvaI 735 CCCGGG C^[CT]CG[GA]G
996 CCCGGG
BbeI 1129 GGCGCC GGCGC^C
2032 GGCGCC
BmyI 127 GGGCCC G[AGT]GC[ACT]^C
1940 GAGCAC
BsmFI 526 GGGAC GGGAC(10/14)
791 GTCCC
BsoBI 735 CCCGGG C^[CT]CG[GA]G
996 CCCGGG
Bsp1286I 127 GGGCCC G[AGT]GC[ACT]^C
1940 GAGCAC
BspMI 514 ACCTGC ACCTGC(4/8)
610 ACCTGC
KasI 1129 GGCGCC G^GCGCC
2032 GGCGCC
MslI 92 CATGTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1293 CACCCAGATG
NarI 1129 GGCGCC GG^CGCC
2032 GGCGCC
PvuII 429 CAGCTG CAG^CTG
1701 CAGCTG
SapI 1259 GAAGAGC GCTCTTC(1/4)
1365 GAAGAGC
SfoI 1129 GGCGCC GGC^GCC
2032 GGCGCC
SmaI 735 CCCGGG CCC^GGG
996 CCCGGG
XmaCI 735 CCCGGG C^CCGGG
996 CCCGGG
XmaI 735 CCCGGG C^CCGGG
996 CCCGGG
XmnI 675 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1869 GAAGTACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI AvaII BalI BanI
BbvI BcnI BfaI BpmI BsaHI
BsaJI BseRI BsiYI BslI BsmAI
BspHI BsrBI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII