##################################
# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AvaI AvaII AviII AvrII
BalI BanII BbeI BbrPI BbsI
BcgI BglI BlnI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsoBI Bsp1286I BspDI BspEI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII CfoI Cfr10I
Cfr13I ClaI CpoI DraII DraIII
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI KasI KpnI KspI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI Sau96I SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I StuI StyI
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 1847 GTCTAC GT^[AC][GT]AC
AflII 963 CTTAAG C^TTAAG
AflIII 571 ACATGT A^C[GA][CT]GT
AspI 986 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1355 GGATCC G^GATCC
BanI 1398 GGTGCC G^G[CT][GA]CC
BbvCI 82 GCTGAGG CCTCAGC(-5/-2)
BciVI 711 GGATAC GTATCC(6/5)
BcnI 1203 CCGGG CC^[GC]GG
BfrI 963 CTTAAG C^TTAAG
BglII 450 AGATCT A^GATCT
BsaI 475 GAGACC GGTCTC(1/5)
BsaJI 57 CCCTGG C^C[ACGT][ACGT]GG
BseRI 85 GAGGAG GAGGAG(10/8)
BsgI 1419 CTGCAC GTGCAG(16/14)
BsmI 1232 GAATGC GAATGC(1/-1)
Bsp1407I 1808 TGTACA T^GTACA
BspHI 231 TCATGA T^CATGA
BspLU11I 571 ACATGT A^CATGT
BsrGI 1808 TGTACA T^GTACA
BstAPI 1111 GCAGTACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 479 CCAAAAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 789 GACCTCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HapII 1203 CCGG C^CGG
HpaII 1203 CCGG C^CGG
MslI 1864 CACACCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1312 CAGCTG C[AC]G^C[GT]G
MspI 1203 CCGG C^CGG
NciI 1203 CCGGG CC^[GC]GG
NheI 1879 GCTAGC G^CTAGC
NspI 571 ACATGT [GA]CATG^[CT]
PflFI 986 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 1052 CTGCAG CTGCA^G
PvuII 1312 CAGCTG CAG^CTG
RcaI 231 TCATGA T^CATGA
ScaI 1113 AGTACT AGT^ACT
SmlI 963 CTTAAG C^T[CT][GA]AG
SpeI 1720 ACTAGT A^CTAGT
SspBI 1808 TGTACA T^GTACA
SwaI 1784 ATTTAAAT ATTT^AAAT
TaqI 1432 TCGA T^CGA
Tth111I 986 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1432 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwI 1356 GATCC GGATC(4/5)
1355 GGATC
AlwNI 467 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
1309 CAGCAGCTG
BbvI 1311 GCAGC GCAGC(8/12)
1120 GCTGC
BsaBI 424 GATAATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1491 GATGATCATC
BsmFI 992 GTCCC GGGAC(10/14)
1239 GTCCC
BspMI 1050 ACCTGC ACCTGC(4/8)
1343 GCAGGT
BsrI 112 ACTGG ACTGG(1/-1)
1215 ACTGG
BstF5I 685 GGATG GGATG(2/0)
1669 GGATG
BstYI 450 AGATCT [GA]^GATC[CT]
1355 GGATCC
EarI 930 CTCTTC CTCTTC(1/4)
900 GAAGAG
Fnu4HI 1120 GCTGC GC^[ACGT]GC
1311 GCAGC
FokI 685 GGATG GGATG(9/13)
1669 GGATG
HaeIII 61 GGCC GG^CC
1083 GGCC
HindIII 483 AAGCTT A^AGCTT
1427 AAGCTT
ItaI 1120 GCTGC GC^[ACGT]GC
1311 GCAGC
Ksp632I 930 CTCTTC CTCTTC(1/4)
900 GAAGAG
MaeII 1066 ACGT A^CGT
1851 ACGT
MamI 424 GATAATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1491 GATGATCATC
MflI 450 AGATCT [GA]^GATC[CT]
1355 GGATCC
PleI 88 GAGTC GAGTC(4/5)
796 GAGTC
SspI 208 AATATT AAT^ATT
1908 AATATT
TseI 1120 GCTGC G^C[AT]GC
1311 GCAGC
Tsp45I 893 GTCAC ^GT[GC]AC
926 GTCAC
TspRI 471 CACTG CA[GC]TG[ACGT][ACGT]^
1405 CACTG
XhoII 450 AGATCT [GA]^GATC[CT]
1355 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AcsI AfaI AluI ApoI BclI
BfaI BsiYI BslI BsmAI BssKI
BstNI Cac8I DdeI DpnII DraI
EcoRII FbaI HinfI HphI MaeI
MaeIII MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI ScrFI SfaNI SfcI
TfiI Tru9I Tsp509I