##################################
# NO CUT ENZYMES (  152 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AciI        
AclI        AcyI        AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AvaI        AvaII       AviII       AvrII       
BalI        BanII       BbeI        BbrPI       BbsI        
BcgI        BglI        BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaHI       
BsaWI       BseAI       BsiEI       BsiHKAI     BsiWI       
BsmBI       BsoBI       Bsp1286I    BspDI       BspEI       
BsrBI       BsrDI       BsrFI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
Cfr13I      ClaI        CpoI        DraII       DraIII      
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FauI        FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HpaI        KasI        KpnI        KspI        MfeI        
MluI        MluNI       MroI        MscI        MunI        
MvnI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacI        SacII       
SalI        SapI        Sau96I      SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SphI        Sse8387I    StuI        StyI        
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccI         1847  GTCTAC               GT^[AC][GT]AC
AflII         963  CTTAAG               C^TTAAG
AflIII        571  ACATGT               A^C[GA][CT]GT
AspI          986  GACTTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        1355  GGATCC               G^GATCC
BanI         1398  GGTGCC               G^G[CT][GA]CC
BbvCI          82  GCTGAGG              CCTCAGC(-5/-2)
BciVI         711  GGATAC               GTATCC(6/5)
BcnI         1203  CCGGG                CC^[GC]GG
BfrI          963  CTTAAG               C^TTAAG
BglII         450  AGATCT               A^GATCT
BsaI          475  GAGACC               GGTCTC(1/5)
BsaJI          57  CCCTGG               C^C[ACGT][ACGT]GG
BseRI          85  GAGGAG               GAGGAG(10/8)
BsgI         1419  CTGCAC               GTGCAG(16/14)
BsmI         1232  GAATGC               GAATGC(1/-1)
Bsp1407I     1808  TGTACA               T^GTACA
BspHI         231  TCATGA               T^CATGA
BspLU11I      571  ACATGT               A^CATGT
BsrGI        1808  TGTACA               T^GTACA
BstAPI       1111  GCAGTACTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         479  CCAAAAGCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI          789  GACCTCAGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HapII        1203  CCGG                 C^CGG
HpaII        1203  CCGG                 C^CGG
MslI         1864  CACACCAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I       1312  CAGCTG               C[AC]G^C[GT]G
MspI         1203  CCGG                 C^CGG
NciI         1203  CCGGG                CC^[GC]GG
NheI         1879  GCTAGC               G^CTAGC
NspI          571  ACATGT               [GA]CATG^[CT]
PflFI         986  GACTTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PstI         1052  CTGCAG               CTGCA^G
PvuII        1312  CAGCTG               CAG^CTG
RcaI          231  TCATGA               T^CATGA
ScaI         1113  AGTACT               AGT^ACT
SmlI          963  CTTAAG               C^T[CT][GA]AG
SpeI         1720  ACTAGT               A^CTAGT
SspBI        1808  TGTACA               T^GTACA
SwaI         1784  ATTTAAAT             ATTT^AAAT
TaqI         1432  TCGA                 T^CGA
Tth111I       986  GACTTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I      1432  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AlwI         1356  GATCC                GGATC(4/5)
             1355  GGATC               
AlwNI         467  CAGCCACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1309  CAGCAGCTG           
BbvI         1311  GCAGC                GCAGC(8/12)
             1120  GCTGC               
BsaBI         424  GATAATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1491  GATGATCATC          
BsmFI         992  GTCCC                GGGAC(10/14)
             1239  GTCCC               
BspMI        1050  ACCTGC               ACCTGC(4/8)
             1343  GCAGGT              
BsrI          112  ACTGG                ACTGG(1/-1)
             1215  ACTGG               
BstF5I        685  GGATG                GGATG(2/0)
             1669  GGATG               
BstYI         450  AGATCT               [GA]^GATC[CT]
             1355  GGATCC              
EarI          930  CTCTTC               CTCTTC(1/4)
              900  GAAGAG              
Fnu4HI       1120  GCTGC                GC^[ACGT]GC
             1311  GCAGC               
FokI          685  GGATG                GGATG(9/13)
             1669  GGATG               
HaeIII         61  GGCC                 GG^CC
             1083  GGCC                
HindIII       483  AAGCTT               A^AGCTT
             1427  AAGCTT              
ItaI         1120  GCTGC                GC^[ACGT]GC
             1311  GCAGC               
Ksp632I       930  CTCTTC               CTCTTC(1/4)
              900  GAAGAG              
MaeII        1066  ACGT                 A^CGT
             1851  ACGT                
MamI          424  GATAATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1491  GATGATCATC          
MflI          450  AGATCT               [GA]^GATC[CT]
             1355  GGATCC              
PleI           88  GAGTC                GAGTC(4/5)
              796  GAGTC               
SspI          208  AATATT               AAT^ATT
             1908  AATATT              
TseI         1120  GCTGC                G^C[AT]GC
             1311  GCAGC               
Tsp45I        893  GTCAC                ^GT[GC]AC
              926  GTCAC               
TspRI         471  CACTG                CA[GC]TG[ACGT][ACGT]^
             1405  CACTG               
XhoII         450  AGATCT               [GA]^GATC[CT]
             1355  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   38 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        BclI        
BfaI        BsiYI       BslI        BsmAI       BssKI       
BstNI       Cac8I       DdeI        DpnII       DraI        
EcoRII      FbaI        HinfI       HphI        MaeI        
MaeIII      MboI        MboII       MnlI        MseI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
RsaI        Sau3AI      ScrFI       SfaNI       SfcI        
TfiI        Tru9I       Tsp509I