################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AvaII AviII AvrII BalI BanII BbeI BbrPI BbsI BcgI BglI BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1286I BspDI BspEI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraII DraIII DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI KspI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI Sau96I SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I StuI StyI Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 1847 GTCTAC GT^[AC][GT]AC AflII 963 CTTAAG C^TTAAG AflIII 571 ACATGT A^C[GA][CT]GT AspI 986 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 1355 GGATCC G^GATCC BanI 1398 GGTGCC G^G[CT][GA]CC BbvCI 82 GCTGAGG CCTCAGC(-5/-2) BciVI 711 GGATAC GTATCC(6/5) BcnI 1203 CCGGG CC^[GC]GG BfrI 963 CTTAAG C^TTAAG BglII 450 AGATCT A^GATCT BsaI 475 GAGACC GGTCTC(1/5) BsaJI 57 CCCTGG C^C[ACGT][ACGT]GG BseRI 85 GAGGAG GAGGAG(10/8) BsgI 1419 CTGCAC GTGCAG(16/14) BsmI 1232 GAATGC GAATGC(1/-1) Bsp1407I 1808 TGTACA T^GTACA BspHI 231 TCATGA T^CATGA BspLU11I 571 ACATGT A^CATGT BsrGI 1808 TGTACA T^GTACA BstAPI 1111 GCAGTACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 479 CCAAAAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DrdI 789 GACCTCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC HapII 1203 CCGG C^CGG HpaII 1203 CCGG C^CGG MslI 1864 CACACCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 1312 CAGCTG C[AC]G^C[GT]G MspI 1203 CCGG C^CGG NciI 1203 CCGGG CC^[GC]GG NheI 1879 GCTAGC G^CTAGC NspI 571 ACATGT [GA]CATG^[CT] PflFI 986 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 1052 CTGCAG CTGCA^G PvuII 1312 CAGCTG CAG^CTG RcaI 231 TCATGA T^CATGA ScaI 1113 AGTACT AGT^ACT SmlI 963 CTTAAG C^T[CT][GA]AG SpeI 1720 ACTAGT A^CTAGT SspBI 1808 TGTACA T^GTACA SwaI 1784 ATTTAAAT ATTT^AAAT TaqI 1432 TCGA T^CGA Tth111I 986 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1432 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AlwI 1356 GATCC GGATC(4/5) 1355 GGATC AlwNI 467 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG 1309 CAGCAGCTG BbvI 1311 GCAGC GCAGC(8/12) 1120 GCTGC BsaBI 424 GATAATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1491 GATGATCATC BsmFI 992 GTCCC GGGAC(10/14) 1239 GTCCC BspMI 1050 ACCTGC ACCTGC(4/8) 1343 GCAGGT BsrI 112 ACTGG ACTGG(1/-1) 1215 ACTGG BstF5I 685 GGATG GGATG(2/0) 1669 GGATG BstYI 450 AGATCT [GA]^GATC[CT] 1355 GGATCC EarI 930 CTCTTC CTCTTC(1/4) 900 GAAGAG Fnu4HI 1120 GCTGC GC^[ACGT]GC 1311 GCAGC FokI 685 GGATG GGATG(9/13) 1669 GGATG HaeIII 61 GGCC GG^CC 1083 GGCC HindIII 483 AAGCTT A^AGCTT 1427 AAGCTT ItaI 1120 GCTGC GC^[ACGT]GC 1311 GCAGC Ksp632I 930 CTCTTC CTCTTC(1/4) 900 GAAGAG MaeII 1066 ACGT A^CGT 1851 ACGT MamI 424 GATAATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1491 GATGATCATC MflI 450 AGATCT [GA]^GATC[CT] 1355 GGATCC PleI 88 GAGTC GAGTC(4/5) 796 GAGTC SspI 208 AATATT AAT^ATT 1908 AATATT TseI 1120 GCTGC G^C[AT]GC 1311 GCAGC Tsp45I 893 GTCAC ^GT[GC]AC 926 GTCAC TspRI 471 CACTG CA[GC]TG[ACGT][ACGT]^ 1405 CACTG XhoII 450 AGATCT [GA]^GATC[CT] 1355 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AcsI AfaI AluI ApoI BclI BfaI BsiYI BslI BsmAI BssKI BstNI Cac8I DdeI DpnII DraI EcoRII FbaI HinfI HphI MaeI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI ScrFI SfaNI SfcI TfiI Tru9I Tsp509I