##################################
# NO CUT ENZYMES ( 126 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AflIII AhdI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvrII BalI BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BlnI BsaAI BsaBI BsaI BseAI
BsiHKAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
DsaI Eam1105I Eco47III Eco81I EcoO65I
EcoRI EcoRV FbaI FseI HaeII
HindIII HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MseI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NspI NspV PacI
PflFI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII ScaI SexAI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SspI StuI SwaI Tru9I
Tsp509I Tth111I XbaI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 181 GACGTC GACGT^C
AccI 163 GTCGAC GT^[AC][GT]AC
AcyI 181 GACGTC G[GA]^CG[CT]C
AfaI 1233 GTAC GT^AC
AgeI 205 ACCGGT A^CCGGT
AviII 479 TGCGCA TGC^GCA
BamHI 75 GGATCC G^GATCC
BanI 687 GGTGCC G^G[CT][GA]CC
BglII 965 AGATCT A^GATCT
BlpI 1260 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1260 GCTCAGC GC^T[ACGT]AGC
BsaHI 181 GACGTC G[GA]^CG[CT]C
BsaWI 205 ACCGGT [AT]^CCGG[AT]
BsmBI 316 CGTCTC CGTCTC(1/5)
BsrBI 80 CCGCTC CCGCTC(-3/-3)
BsrFI 205 ACCGGT [GA]^CCGG[CT]
BstAPI 922 GCAGGCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1260 GCTCAGC GC^T[ACGT]AGC
Cfr10I 205 ACCGGT [GA]^CCGG[CT]
EagI 61 CGGCCG C^GGCCG
EclXI 61 CGGCCG C^GGCCG
Eco52I 61 CGGCCG C^GGCCG
EcoNI 885 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 293 CCTTGG C^C[AT][AT]GG
EcoT22I 614 ATGCAT ATGCA^T
FspI 479 TGCGCA TGC^GCA
HgaI 315 GCGTC GACGC(5/10)
Hin1I 181 GACGTC G[GA]^CG[CT]C
MaeII 182 ACGT A^CGT
MslI 1248 CATTGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NsiI 614 ATGCAT ATGCA^T
PaeR7I 24 CTCGAG C^TCGAG
PinAI 205 ACCGGT A^CCGGT
PshAI 291 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 817 CAGCTG CAG^CTG
RsaI 1233 GTAC GT^AC
SalI 163 GTCGAC G^TCGAC
SapI 240 GCTCTTC GCTCTTC(1/4)
SbfI 709 CCTGCAGG CCTGCA^GG
SfaNI 613 GATGC GCATC(5/9)
SfiI 1128 GGCCCAGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 709 CCTGCAGG CCTGCA^GG
StyI 293 CCTTGG C^C[AT][AT]GG
XcmI 33 CCAGGCGGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 24 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccII 19 CGCG CG^CG
121 CGCG
ApaI 102 GGGCCC GGGCC^C
532 GGGCCC
AvaI 24 CTCGAG C^[CT]CG[GA]G
1361 CCCGAG
BbsI 178 GAAGAC GAAGAC(2/6)
1011 GAAGAC
BcnI 932 CCGGG CC^[GC]GG
1085 CCCGG
BglI 1129 GCCCAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1209 GCCCCGAAGGC
BpuAI 178 GAAGAC GAAGAC(2/6)
1011 GAAGAC
BsiEI 61 CGGCCG CG[GA][CT]^CG
203 CGACCG
BsmAI 1319 GAGAC GTCTC(1/5)
317 GTCTC
BsoBI 24 CTCGAG C^[CT]CG[GA]G
1361 CCCGAG
BstUI 19 CGCG CG^CG
121 CGCG
EaeI 61 CGGCCG [CT]^GGCC[GA]
655 CGGCCA
HincII 163 GTCGAC GT[CT]^[GA]AC
337 GTTGAC
HindII 163 GTCGAC GT[CT]^[GA]AC
337 GTTGAC
MvnI 19 CGCG CG^CG
121 CGCG
NciI 932 CCGGG CC^[GC]GG
1085 CCCGG
NlaIII 769 CATG CATG^
916 CATG
PpuMI 289 AGGACCT [GA]G^G[AT]CC[CT]
398 AGGTCCC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BanII BbvI BmyI BpmI BsaJI
BseRI BsgI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MflI MnlI MspA1I MspI MvaI
MwoI NdeII NlaIV PflMI PleI
PstI Sau3AI Sau96I ScrFI SfcI
SmlI TaqI TfiI TseI Tsp45I
TspRI TthHB8I Van91I XhoII