################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiHKAI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoT22I FauI FbaI FseI FspI HincII HindII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccII 25 CGCG CG^CG AcsI 1414 AAATTC [GA]^AATT[CT] AcyI 9 GGCGTC G[GA]^CG[CT]C AflIII 1337 ACGTGT A^C[GA][CT]GT ApaI 1646 GGGCCC GGGCC^C ApoI 1414 AAATTC [GA]^AATT[CT] BcgI 351 GCACAAACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaHI 9 GGCGTC G[GA]^CG[CT]C BsgI 284 GTGCAG GTGCAG(16/14) BsiEI 43 CGGCCG CG[GA][CT]^CG BsiWI 189 CGTACG C^GTACG BsrBI 30 GAGCGG CCGCTC(-3/-3) BsrDI 898 CATTGC GCAATG(2/0) BstBI 543 TTCGAA TT^CGAA BstUI 25 CGCG CG^CG DraII 72 AGGCCCC [GA]G^G[ACGT]CC[CT] EagI 43 CGGCCG C^GGCCG EclXI 43 CGGCCG C^GGCCG Eco52I 43 CGGCCG C^GGCCG EcoO109I 72 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoRV 1493 GATATC GAT^ATC HaeII 55 GGCGCT [GA]GCGC^[CT] HapII 77 CCGG C^CGG Hin1I 9 GGCGTC G[GA]^CG[CT]C HindIII 593 AAGCTT A^AGCTT HpaII 77 CCGG C^CGG MslI 982 CATGTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 1638 CAGCTG C[AC]G^C[GT]G MspI 77 CCGG C^CGG MvnI 25 CGCG CG^CG NcoI 51 CCATGG C^CATGG NspV 543 TTCGAA TT^CGAA PflMI 1637 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 745 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 1593 CTGCAG CTGCA^G PvuII 1638 CAGCTG CAG^CTG SfuI 543 TTCGAA TT^CGAA SmaI 76 CCCGGG CCC^GGG SmlI 95 CTCAAG C^T[CT][GA]AG StuI 153 AGGCCT AGG^CCT Van91I 1637 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 76 CCCGGG C^CCGGG XmaI 76 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AlwNI 903 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG 1596 CAGTCACTG Asp700I 398 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1388 GAAGCGATTC AvaI 76 CCCGGG C^[CT]CG[GA]G 118 CTCGGG AvaII 194 GGACC G^G[AT]CC 816 GGACC BalI 588 TGGCCA TGG^CCA 1581 TGGCCA BanI 1037 GGTGCC G^G[CT][GA]CC 1160 GGTGCC BcnI 76 CCCGG CC^[GC]GG 77 CCGGG BfaI 278 CTAG C^TAG 392 CTAG BpmI 230 CTGGAG CTGGAG(16/14) 1281 CTGGAG BsoBI 76 CCCGGG C^[CT]CG[GA]G 118 CTCGGG BspHI 469 TCATGA T^CATGA 555 TCATGA BstYI 474 AGATCC [GA]^GATC[CT] 1009 GGATCT CfoI 24 GCGC GCG^C 56 GCGC DsaI 51 CCATGG C^C[GA][CT]GG 1397 CCACGG EcoT14I 51 CCATGG C^C[AT][AT]GG 69 CCAAGG HgaI 10 GCGTC GACGC(5/10) 1270 GCGTC HhaI 24 GCGC GCG^C 56 GCGC HinP1I 24 GCGC G^CGC 56 GCGC MaeI 278 CTAG C^TAG 392 CTAG MflI 474 AGATCC [GA]^GATC[CT] 1009 GGATCT MluNI 588 TGGCCA TGG^CCA 1581 TGGCCA MscI 588 TGGCCA TGG^CCA 1581 TGGCCA NciI 76 CCCGG CC^[GC]GG 77 CCGGG NdeI 323 CATATG CA^TATG 1326 CATATG PleI 1020 GACTC GAGTC(4/5) 1060 GACTC RcaI 469 TCATGA T^CATGA 555 TCATGA StyI 51 CCATGG C^C[AT][AT]GG 69 CCAAGG XhoII 474 AGATCC [GA]^GATC[CT] 1009 GGATCT XmnI 398 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1388 GAAGCGATTC ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AciI AfaI AluI AlwI BanII BbvI BmyI BsaJI BseRI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EaeI EarI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I