##################################
# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AviII
AvrII BamHI BbeI BbrPI BbsI
BbvCI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsiHKAI BsmI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoT22I FauI FbaI FseI
FspI HincII HindII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XcmI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccII 25 CGCG CG^CG
AcsI 1414 AAATTC [GA]^AATT[CT]
AcyI 9 GGCGTC G[GA]^CG[CT]C
AflIII 1337 ACGTGT A^C[GA][CT]GT
ApaI 1646 GGGCCC GGGCC^C
ApoI 1414 AAATTC [GA]^AATT[CT]
BcgI 351 GCACAAACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI 9 GGCGTC G[GA]^CG[CT]C
BsgI 284 GTGCAG GTGCAG(16/14)
BsiEI 43 CGGCCG CG[GA][CT]^CG
BsiWI 189 CGTACG C^GTACG
BsrBI 30 GAGCGG CCGCTC(-3/-3)
BsrDI 898 CATTGC GCAATG(2/0)
BstBI 543 TTCGAA TT^CGAA
BstUI 25 CGCG CG^CG
DraII 72 AGGCCCC [GA]G^G[ACGT]CC[CT]
EagI 43 CGGCCG C^GGCCG
EclXI 43 CGGCCG C^GGCCG
Eco52I 43 CGGCCG C^GGCCG
EcoO109I 72 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRV 1493 GATATC GAT^ATC
HaeII 55 GGCGCT [GA]GCGC^[CT]
HapII 77 CCGG C^CGG
Hin1I 9 GGCGTC G[GA]^CG[CT]C
HindIII 593 AAGCTT A^AGCTT
HpaII 77 CCGG C^CGG
MslI 982 CATGTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1638 CAGCTG C[AC]G^C[GT]G
MspI 77 CCGG C^CGG
MvnI 25 CGCG CG^CG
NcoI 51 CCATGG C^CATGG
NspV 543 TTCGAA TT^CGAA
PflMI 1637 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 745 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1593 CTGCAG CTGCA^G
PvuII 1638 CAGCTG CAG^CTG
SfuI 543 TTCGAA TT^CGAA
SmaI 76 CCCGGG CCC^GGG
SmlI 95 CTCAAG C^T[CT][GA]AG
StuI 153 AGGCCT AGG^CCT
Van91I 1637 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 76 CCCGGG C^CCGGG
XmaI 76 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AlwNI 903 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
1596 CAGTCACTG
Asp700I 398 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1388 GAAGCGATTC
AvaI 76 CCCGGG C^[CT]CG[GA]G
118 CTCGGG
AvaII 194 GGACC G^G[AT]CC
816 GGACC
BalI 588 TGGCCA TGG^CCA
1581 TGGCCA
BanI 1037 GGTGCC G^G[CT][GA]CC
1160 GGTGCC
BcnI 76 CCCGG CC^[GC]GG
77 CCGGG
BfaI 278 CTAG C^TAG
392 CTAG
BpmI 230 CTGGAG CTGGAG(16/14)
1281 CTGGAG
BsoBI 76 CCCGGG C^[CT]CG[GA]G
118 CTCGGG
BspHI 469 TCATGA T^CATGA
555 TCATGA
BstYI 474 AGATCC [GA]^GATC[CT]
1009 GGATCT
CfoI 24 GCGC GCG^C
56 GCGC
DsaI 51 CCATGG C^C[GA][CT]GG
1397 CCACGG
EcoT14I 51 CCATGG C^C[AT][AT]GG
69 CCAAGG
HgaI 10 GCGTC GACGC(5/10)
1270 GCGTC
HhaI 24 GCGC GCG^C
56 GCGC
HinP1I 24 GCGC G^CGC
56 GCGC
MaeI 278 CTAG C^TAG
392 CTAG
MflI 474 AGATCC [GA]^GATC[CT]
1009 GGATCT
MluNI 588 TGGCCA TGG^CCA
1581 TGGCCA
MscI 588 TGGCCA TGG^CCA
1581 TGGCCA
NciI 76 CCCGG CC^[GC]GG
77 CCGGG
NdeI 323 CATATG CA^TATG
1326 CATATG
PleI 1020 GACTC GAGTC(4/5)
1060 GACTC
RcaI 469 TCATGA T^CATGA
555 TCATGA
StyI 51 CCATGG C^C[AT][AT]GG
69 CCAAGG
XhoII 474 AGATCC [GA]^GATC[CT]
1009 GGATCT
XmnI 398 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1388 GAAGCGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AfaI AluI AlwI BanII
BbvI BmyI BsaJI BseRI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EaeI EarI
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I