##################################
# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BalI BamHI BbrPI BbsI BbvCI
BciVI BfrI BglI BlnI BpuAI
BsaAI BsaBI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspEI BspHI
BspLU11I BsrDI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstXI BstZ17I Bsu36I CpoI DraI
DraII DrdI DsaI EagI Eam1105I
EclXI Eco52I Eco81I EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FseI
FspI HindIII HpaI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MspA1I MunI NcoI NdeI
NheI NotI NruI NsiI PacI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SapI ScaI SfiI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
StuI StyI SwaI Van91I XbaI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 101 GTCTAC GT^[AC][GT]AC
AcsI 1145 GAATTT [GA]^AATT[CT]
Aor51HI 13 AGCGCT AGC^GCT
ApoI 1145 GAATTT [GA]^AATT[CT]
AspHI 498 GAGCTC G[AT]GC[AT]^C
AvaII 289 GGTCC G^G[AT]CC
BanI 273 GGCGCC G^G[CT][GA]CC
BanII 498 GAGCTC G[GA]GC[CT]^C
BbeI 273 GGCGCC GGCGC^C
BcgI 133 GCAGGATGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1459 TGATCA T^GATCA
BfaI 241 CTAG C^TAG
BglII 1250 AGATCT A^GATCT
BlpI 495 GCTGAGC GC^T[ACGT]AGC
BmyI 498 GAGCTC G[AGT]GC[ACT]^C
Bpu1102I 495 GCTGAGC GC^T[ACGT]AGC
BsiHKAI 498 GAGCTC G[AT]GC[AT]^C
Bsp1286I 498 GAGCTC G[AGT]GC[ACT]^C
BspDI 864 ATCGAT AT^CGAT
BspMI 575 GCAGGT ACCTGC(4/8)
BsrBI 618 GAGCGG CCGCTC(-3/-3)
BstBI 90 TTCGAA TT^CGAA
CelII 495 GCTGAGC GC^T[ACGT]AGC
ClaI 864 ATCGAT AT^CGAT
DraIII 1510 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 13 AGCGCT AGC^GCT
FbaI 1459 TGATCA T^GATCA
HgaI 366 GACGC GACGC(5/10)
KasI 273 GGCGCC G^GCGCC
MaeI 241 CTAG C^TAG
NarI 273 GGCGCC GG^CGCC
NspI 854 GCATGT [GA]CATG^[CT]
NspV 90 TTCGAA TT^CGAA
SacI 498 GAGCTC GAGCT^C
SbfI 1190 CCTGCAGG CCTGCA^GG
SexAI 105 ACCAGGT A^CC[AT]GGT
SfoI 273 GGCGCC GGC^GCC
SfuI 90 TTCGAA TT^CGAA
Sse8387I 1190 CCTGCAGG CCTGCA^GG
XcmI 1172 CCAGGACACTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 37 CGCG CG^CG
529 CGCG
AcyI 273 GGCGCC G[GA]^CG[CT]C
366 GACGCC
AlwNI 420 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
825 CAGGAGCTG
AspI 191 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
284 GACCCGGTC
AvaI 468 CTCGAG C^[CT]CG[GA]G
501 CTCGAG
BsaHI 273 GGCGCC G[GA]^CG[CT]C
366 GACGCC
BsaI 5 GAGACC GGTCTC(1/5)
1440 GAGACC
BsmFI 622 GGGAC GGGAC(10/14)
1112 GTCCC
BsoBI 468 CTCGAG C^[CT]CG[GA]G
501 CTCGAG
BsrFI 163 GCCGGC [GA]^CCGG[CT]
571 GCCGGC
BstUI 37 CGCG CG^CG
529 CGCG
Cfr10I 163 GCCGGC [GA]^CCGG[CT]
571 GCCGGC
Hin1I 273 GGCGCC G[GA]^CG[CT]C
366 GACGCC
HincII 726 GTTGAC GT[CT]^[GA]AC
1398 GTTGAC
HindII 726 GTTGAC GT[CT]^[GA]AC
1398 GTTGAC
MslI 1233 CACCTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1479 CATCACGATG
MvnI 37 CGCG CG^CG
529 CGCG
NaeI 163 GCCGGC GCC^GGC
571 GCCGGC
NgoMIV 163 GCCGGC G^CCGGC
571 GCCGGC
NlaIV 147 GGCTCC GG[ACGT]^[ACGT]CC
273 GGCGCC
PaeR7I 468 CTCGAG C^TCGAG
501 CTCGAG
PflFI 191 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
284 GACCCGGTC
Tth111I 191 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
284 GACCCGGTC
XhoI 468 CTCGAG C^TCGAG
501 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BcnI BpmI BsaJI BsaWI BseRI
BsgI BsiYI BslI BsmAI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EaeI EarI
EcoNI EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MseI MspI MvaI MwoI
NciI NdeII NlaIII PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII