################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BciVI BfrI BglI BlnI BpuAI BsaAI BsaBI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspEI BspHI BspLU11I BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstXI BstZ17I Bsu36I CpoI DraI DraII DrdI DsaI EagI Eam1105I EclXI Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI FspI HindIII HpaI KpnI KspI MamI MfeI MluI MluNI MroI MscI MspA1I MunI NcoI NdeI NheI NotI NruI NsiI PacI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI ScaI SfiI SgrAI SmaI SnaBI SpeI SphI SspBI SspI StuI StyI SwaI Van91I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 101 GTCTAC GT^[AC][GT]AC AcsI 1145 GAATTT [GA]^AATT[CT] Aor51HI 13 AGCGCT AGC^GCT ApoI 1145 GAATTT [GA]^AATT[CT] AspHI 498 GAGCTC G[AT]GC[AT]^C AvaII 289 GGTCC G^G[AT]CC BanI 273 GGCGCC G^G[CT][GA]CC BanII 498 GAGCTC G[GA]GC[CT]^C BbeI 273 GGCGCC GGCGC^C BcgI 133 GCAGGATGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1459 TGATCA T^GATCA BfaI 241 CTAG C^TAG BglII 1250 AGATCT A^GATCT BlpI 495 GCTGAGC GC^T[ACGT]AGC BmyI 498 GAGCTC G[AGT]GC[ACT]^C Bpu1102I 495 GCTGAGC GC^T[ACGT]AGC BsiHKAI 498 GAGCTC G[AT]GC[AT]^C Bsp1286I 498 GAGCTC G[AGT]GC[ACT]^C BspDI 864 ATCGAT AT^CGAT BspMI 575 GCAGGT ACCTGC(4/8) BsrBI 618 GAGCGG CCGCTC(-3/-3) BstBI 90 TTCGAA TT^CGAA CelII 495 GCTGAGC GC^T[ACGT]AGC ClaI 864 ATCGAT AT^CGAT DraIII 1510 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 13 AGCGCT AGC^GCT FbaI 1459 TGATCA T^GATCA HgaI 366 GACGC GACGC(5/10) KasI 273 GGCGCC G^GCGCC MaeI 241 CTAG C^TAG NarI 273 GGCGCC GG^CGCC NspI 854 GCATGT [GA]CATG^[CT] NspV 90 TTCGAA TT^CGAA SacI 498 GAGCTC GAGCT^C SbfI 1190 CCTGCAGG CCTGCA^GG SexAI 105 ACCAGGT A^CC[AT]GGT SfoI 273 GGCGCC GGC^GCC SfuI 90 TTCGAA TT^CGAA Sse8387I 1190 CCTGCAGG CCTGCA^GG XcmI 1172 CCAGGACACTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccII 37 CGCG CG^CG 529 CGCG AcyI 273 GGCGCC G[GA]^CG[CT]C 366 GACGCC AlwNI 420 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG 825 CAGGAGCTG AspI 191 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC 284 GACCCGGTC AvaI 468 CTCGAG C^[CT]CG[GA]G 501 CTCGAG BsaHI 273 GGCGCC G[GA]^CG[CT]C 366 GACGCC BsaI 5 GAGACC GGTCTC(1/5) 1440 GAGACC BsmFI 622 GGGAC GGGAC(10/14) 1112 GTCCC BsoBI 468 CTCGAG C^[CT]CG[GA]G 501 CTCGAG BsrFI 163 GCCGGC [GA]^CCGG[CT] 571 GCCGGC BstUI 37 CGCG CG^CG 529 CGCG Cfr10I 163 GCCGGC [GA]^CCGG[CT] 571 GCCGGC Hin1I 273 GGCGCC G[GA]^CG[CT]C 366 GACGCC HincII 726 GTTGAC GT[CT]^[GA]AC 1398 GTTGAC HindII 726 GTTGAC GT[CT]^[GA]AC 1398 GTTGAC MslI 1233 CACCTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1479 CATCACGATG MvnI 37 CGCG CG^CG 529 CGCG NaeI 163 GCCGGC GCC^GGC 571 GCCGGC NgoMIV 163 GCCGGC G^CCGGC 571 GCCGGC NlaIV 147 GGCTCC GG[ACGT]^[ACGT]CC 273 GGCGCC PaeR7I 468 CTCGAG C^TCGAG 501 CTCGAG PflFI 191 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC 284 GACCCGGTC Tth111I 191 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC 284 GACCCGGTC XhoI 468 CTCGAG C^TCGAG 501 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AciI AfaI AluI AlwI BbvI BcnI BpmI BsaJI BsaWI BseRI BsgI BsiYI BslI BsmAI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII EaeI EarI EcoNI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MseI MspI MvaI MwoI NciI NdeII NlaIII PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII