################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## AatII AccIII AclI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AspEI AspI AviII AvrII BamHI BbeI BbrPI BcgI BclI BfrI BglI BglII BlnI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BspDI BspEI BspHI BsrBI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV FbaI FseI FspI Hin1I HpaI KasI KspI MamI MluI MroI NarI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 1439 GGTACC G^GTACC AccII 339 CGCG CG^CG AseI 1593 ATTAAT AT^TAAT AsnI 1593 ATTAAT AT^TAAT Asp700I 457 GAAATAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1439 GGTACC G^GTACC BbsI 997 GAAGAC GAAGAC(2/6) BbvCI 1531 GCTGAGG CCTCAGC(-5/-2) BciVI 780 GTATCC GTATCC(6/5) BcnI 35 CCGGG CC^[GC]GG BlpI 278 GCTCAGC GC^T[ACGT]AGC Bpu1102I 278 GCTCAGC GC^T[ACGT]AGC BpuAI 997 GAAGAC GAAGAC(2/6) BsmBI 10 GAGACG CGTCTC(1/5) BspLU11I 2464 ACATGT A^CATGT BspMI 15 GCAGGT ACCTGC(4/8) BsrDI 2020 GCAATG GCAATG(2/0) BstAPI 94 GCATCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 339 CGCG CG^CG CelII 278 GCTCAGC GC^T[ACGT]AGC CfoI 1511 GCGC GCG^C DraI 2762 TTTAAA TTT^AAA DsaI 86 CCATGG C^C[GA][CT]GG HaeII 1510 GGCGCT [GA]GCGC^[CT] HhaI 1511 GCGC GCG^C HinP1I 1511 GCGC G^CGC HindIII 2030 AAGCTT A^AGCTT KpnI 1439 GGTACC GGTAC^C MvnI 339 CGCG CG^CG NciI 35 CCGGG CC^[GC]GG NcoI 86 CCATGG C^CATGG PflMI 1907 CCACAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 12 GACGCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1593 ATTAAT AT^TAAT SacI 114 GAGCTC GAGCT^C SapI 1138 GAAGAGC GCTCTTC(1/4) SexAI 1385 ACCAGGT A^CC[AT]GGT TaqI 372 TCGA T^CGA TthHB8I 372 TCGA T^CGA Van91I 1907 CCACAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 457 GAAATAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 45 enzymes) # ################################### AccI 1282 GTCTAC GT^[AC][GT]AC 2005 GTCTAC AflIII 2345 ACACGT A^C[GA][CT]GT 2464 ACATGT AlwI 2085 GATCC GGATC(4/5) 1813 GGATC AlwNI 359 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG 1387 CAGGTTCTG AocI 1353 CCTGAGG CC^T[ACGT]AGG 2076 CCTGAGG AvaI 133 CTCGGG C^[CT]CG[GA]G 717 CTCGGG BalI 1208 TGGCCA TGG^CCA 2643 TGGCCA BanI 733 GGTGCC G^G[CT][GA]CC 1439 GGTACC BsgI 1503 CTGCAC GTGCAG(16/14) 2375 GTGCAG BsmI 1312 GAATGC GAATGC(1/-1) 1611 GCATTC BsoBI 133 CTCGGG C^[CT]CG[GA]G 717 CTCGGG Bsp1407I 1679 TGTACA T^GTACA 2461 TGTACA BsrFI 3 GCCGGC [GA]^CCGG[CT] 685 GCCGGC BsrGI 1679 TGTACA T^GTACA 2461 TGTACA BstYI 1813 GGATCT [GA]^GATC[CT] 2084 AGATCC Bsu36I 1353 CCTGAGG CC^T[ACGT]AGG 2076 CCTGAGG Cfr10I 3 GCCGGC [GA]^CCGG[CT] 685 GCCGGC DraII 17 AGGTCCC [GA]G^G[ACGT]CC[CT] 1064 GGGACCC EarI 1949 CTCTTC CTCTTC(1/4) 1138 GAAGAG Eco81I 1353 CCTGAGG CC^T[ACGT]AGG 2076 CCTGAGG EcoO109I 17 AGGTCCC [GA]G^G[ACGT]CC[CT] 1064 GGGACCC EcoT22I 1561 ATGCAT ATGCA^T 2272 ATGCAT FauI 126 CCCGC CCCGC(4/6) 337 CCCGC HgaI 12 GACGC GACGC(5/10) 340 GCGTC HincII 663 GTTGAC GT[CT]^[GA]AC 1836 GTCAAC HindII 663 GTTGAC GT[CT]^[GA]AC 1836 GTCAAC Ksp632I 1949 CTCTTC CTCTTC(1/4) 1138 GAAGAG MfeI 2211 CAATTG C^AATTG 2754 CAATTG MflI 1813 GGATCT [GA]^GATC[CT] 2084 AGATCC MluNI 1208 TGGCCA TGG^CCA 2643 TGGCCA MscI 1208 TGGCCA TGG^CCA 2643 TGGCCA MslI 151 CATGACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2279 CACATAGATG MunI 2211 CAATTG C^AATTG 2754 CAATTG NaeI 3 GCCGGC GCC^GGC 685 GCCGGC NgoMIV 3 GCCGGC G^CCGGC 685 GCCGGC NsiI 1561 ATGCAT ATGCA^T 2272 ATGCAT NspI 814 GCATGT [GA]CATG^[CT] 2464 ACATGT PpuMI 17 AGGTCCC [GA]G^G[AT]CC[CT] 1064 GGGACCC PstI 1616 CTGCAG CTGCA^G 1817 CTGCAG SfcI 1616 CTGCAG C^T[GA][CT]AG 1817 CTGCAG SspBI 1679 TGTACA T^GTACA 2461 TGTACA TfiI 1568 GAATC G^A[AT]TC 1822 GATTC Tsp45I 158 GTGAC ^GT[GC]AC 497 GTGAC XbaI 2229 TCTAGA T^CTAGA 2557 TCTAGA XhoII 1813 GGATCT [GA]^GATC[CT] 2084 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI AspHI AvaII BanII BbvI BfaI BmyI BpmI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EaeI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SmlI StyI Tru9I TseI Tsp509I TspRI