##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AspEI AspI AviII
AvrII BamHI BbeI BbrPI BcgI
BclI BfrI BglI BglII BlnI
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BspDI BspEI BspHI
BsrBI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV FbaI FseI FspI
Hin1I HpaI KasI KspI MamI
MluI MroI NarI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 1439 GGTACC G^GTACC
AccII 339 CGCG CG^CG
AseI 1593 ATTAAT AT^TAAT
AsnI 1593 ATTAAT AT^TAAT
Asp700I 457 GAAATAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1439 GGTACC G^GTACC
BbsI 997 GAAGAC GAAGAC(2/6)
BbvCI 1531 GCTGAGG CCTCAGC(-5/-2)
BciVI 780 GTATCC GTATCC(6/5)
BcnI 35 CCGGG CC^[GC]GG
BlpI 278 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 278 GCTCAGC GC^T[ACGT]AGC
BpuAI 997 GAAGAC GAAGAC(2/6)
BsmBI 10 GAGACG CGTCTC(1/5)
BspLU11I 2464 ACATGT A^CATGT
BspMI 15 GCAGGT ACCTGC(4/8)
BsrDI 2020 GCAATG GCAATG(2/0)
BstAPI 94 GCATCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 339 CGCG CG^CG
CelII 278 GCTCAGC GC^T[ACGT]AGC
CfoI 1511 GCGC GCG^C
DraI 2762 TTTAAA TTT^AAA
DsaI 86 CCATGG C^C[GA][CT]GG
HaeII 1510 GGCGCT [GA]GCGC^[CT]
HhaI 1511 GCGC GCG^C
HinP1I 1511 GCGC G^CGC
HindIII 2030 AAGCTT A^AGCTT
KpnI 1439 GGTACC GGTAC^C
MvnI 339 CGCG CG^CG
NciI 35 CCGGG CC^[GC]GG
NcoI 86 CCATGG C^CATGG
PflMI 1907 CCACAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 12 GACGCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1593 ATTAAT AT^TAAT
SacI 114 GAGCTC GAGCT^C
SapI 1138 GAAGAGC GCTCTTC(1/4)
SexAI 1385 ACCAGGT A^CC[AT]GGT
TaqI 372 TCGA T^CGA
TthHB8I 372 TCGA T^CGA
Van91I 1907 CCACAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 457 GAAATAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 45 enzymes) #
###################################
AccI 1282 GTCTAC GT^[AC][GT]AC
2005 GTCTAC
AflIII 2345 ACACGT A^C[GA][CT]GT
2464 ACATGT
AlwI 2085 GATCC GGATC(4/5)
1813 GGATC
AlwNI 359 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
1387 CAGGTTCTG
AocI 1353 CCTGAGG CC^T[ACGT]AGG
2076 CCTGAGG
AvaI 133 CTCGGG C^[CT]CG[GA]G
717 CTCGGG
BalI 1208 TGGCCA TGG^CCA
2643 TGGCCA
BanI 733 GGTGCC G^G[CT][GA]CC
1439 GGTACC
BsgI 1503 CTGCAC GTGCAG(16/14)
2375 GTGCAG
BsmI 1312 GAATGC GAATGC(1/-1)
1611 GCATTC
BsoBI 133 CTCGGG C^[CT]CG[GA]G
717 CTCGGG
Bsp1407I 1679 TGTACA T^GTACA
2461 TGTACA
BsrFI 3 GCCGGC [GA]^CCGG[CT]
685 GCCGGC
BsrGI 1679 TGTACA T^GTACA
2461 TGTACA
BstYI 1813 GGATCT [GA]^GATC[CT]
2084 AGATCC
Bsu36I 1353 CCTGAGG CC^T[ACGT]AGG
2076 CCTGAGG
Cfr10I 3 GCCGGC [GA]^CCGG[CT]
685 GCCGGC
DraII 17 AGGTCCC [GA]G^G[ACGT]CC[CT]
1064 GGGACCC
EarI 1949 CTCTTC CTCTTC(1/4)
1138 GAAGAG
Eco81I 1353 CCTGAGG CC^T[ACGT]AGG
2076 CCTGAGG
EcoO109I 17 AGGTCCC [GA]G^G[ACGT]CC[CT]
1064 GGGACCC
EcoT22I 1561 ATGCAT ATGCA^T
2272 ATGCAT
FauI 126 CCCGC CCCGC(4/6)
337 CCCGC
HgaI 12 GACGC GACGC(5/10)
340 GCGTC
HincII 663 GTTGAC GT[CT]^[GA]AC
1836 GTCAAC
HindII 663 GTTGAC GT[CT]^[GA]AC
1836 GTCAAC
Ksp632I 1949 CTCTTC CTCTTC(1/4)
1138 GAAGAG
MfeI 2211 CAATTG C^AATTG
2754 CAATTG
MflI 1813 GGATCT [GA]^GATC[CT]
2084 AGATCC
MluNI 1208 TGGCCA TGG^CCA
2643 TGGCCA
MscI 1208 TGGCCA TGG^CCA
2643 TGGCCA
MslI 151 CATGACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2279 CACATAGATG
MunI 2211 CAATTG C^AATTG
2754 CAATTG
NaeI 3 GCCGGC GCC^GGC
685 GCCGGC
NgoMIV 3 GCCGGC G^CCGGC
685 GCCGGC
NsiI 1561 ATGCAT ATGCA^T
2272 ATGCAT
NspI 814 GCATGT [GA]CATG^[CT]
2464 ACATGT
PpuMI 17 AGGTCCC [GA]G^G[AT]CC[CT]
1064 GGGACCC
PstI 1616 CTGCAG CTGCA^G
1817 CTGCAG
SfcI 1616 CTGCAG C^T[GA][CT]AG
1817 CTGCAG
SspBI 1679 TGTACA T^GTACA
2461 TGTACA
TfiI 1568 GAATC G^A[AT]TC
1822 GATTC
Tsp45I 158 GTGAC ^GT[GC]AC
497 GTGAC
XbaI 2229 TCTAGA T^CTAGA
2557 TCTAGA
XhoII 1813 GGATCT [GA]^GATC[CT]
2084 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI AvaII BanII BbvI BfaI
BmyI BpmI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EaeI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI Tru9I TseI
Tsp509I TspRI