##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AgeI AhdI Aor51HI ApaI AscI
Asp700I AspEI AspI AvaI AviII
BbvCI BcgI BclI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsoBI
BspDI BspEI BspHI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Cfr10I ClaI CpoI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO109I EcoO65I EcoRI EcoRV
FbaI FseI FspI HincII HindII
HpaI KspI MfeI MluI MroI
MunI MvnI NaeI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmeI PpuMI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfcI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI Tth111I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 1227 GGTACC G^GTACC
AflII 935 CTTAAG C^TTAAG
Alw44I 1711 GTGCAC G^TGCAC
AlwNI 189 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1711 GTGCAC G^TGCAC
AseI 1547 ATTAAT AT^TAAT
AsnI 1547 ATTAAT AT^TAAT
Asp718I 1227 GGTACC G^GTACC
AvrII 1334 CCTAGG C^CTAGG
BalI 546 TGGCCA TGG^CCA
BanII 161 GAGCTC G[GA]GC[CT]^C
BbeI 230 GGCGCC GGCGC^C
BbrPI 1495 CACGTG CAC^GTG
BbsI 565 GAAGAC GAAGAC(2/6)
BciVI 753 GTATCC GTATCC(6/5)
BcnI 560 CCGGG CC^[GC]GG
BfrI 935 CTTAAG C^TTAAG
BglI 1451 GCCACTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1534 AGATCT A^GATCT
BlnI 1334 CCTAGG C^CTAGG
BpuAI 565 GAAGAC GAAGAC(2/6)
BsaAI 1495 CACGTG [CT]AC^GT[GA]
BsaBI 1369 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 1640 TGTACA T^GTACA
BsrBI 13 GAGCGG CCGCTC(-3/-3)
BsrGI 1640 TGTACA T^GTACA
EaeI 546 TGGCCA [CT]^GGCC[GA]
EarI 794 CTCTTC CTCTTC(1/4)
EcoNI 1474 CCTGAGGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 1240 CCCGC CCCGC(4/6)
HaeII 230 GGCGCC [GA]GCGC^[CT]
HapII 560 CCGG C^CGG
HgaI 1 GACGC GACGC(5/10)
HpaII 560 CCGG C^CGG
KasI 230 GGCGCC G^GCGCC
KpnI 1227 GGTACC GGTAC^C
Ksp632I 794 CTCTTC CTCTTC(1/4)
MamI 1369 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 546 TGGCCA TGG^CCA
MscI 546 TGGCCA TGG^CCA
MspA1I 216 CAGCTG C[AC]G^C[GT]G
MspI 560 CCGG C^CGG
NarI 230 GGCGCC GG^CGCC
NciI 560 CCGGG CC^[GC]GG
PmaCI 1495 CACGTG CAC^GTG
PmlI 1495 CACGTG CAC^GTG
PshAI 1519 GACTTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1547 ATTAAT AT^TAAT
PvuII 216 CAGCTG CAG^CTG
SacI 161 GAGCTC GAGCT^C
SapI 793 GCTCTTC GCTCTTC(1/4)
SfoI 230 GGCGCC GGC^GCC
SspBI 1640 TGTACA T^GTACA
TaqI 1666 TCGA T^CGA
TthHB8I 1666 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AciI 1241 CCGC CCGC(-3/-1)
15 GCGG
AcsI 691 AAATTT [GA]^AATT[CT]
1341 AAATTT
AcyI 1 GACGCC G[GA]^CG[CT]C
230 GGCGCC
AflIII 641 ACATGT A^C[GA][CT]GT
1643 ACATGT
AocI 119 CCTCAGG CC^T[ACGT]AGG
1474 CCTGAGG
ApoI 691 AAATTT [GA]^AATT[CT]
1341 AAATTT
BamHI 1148 GGATCC G^GATCC
1560 GGATCC
BlpI 333 GCTCAGC GC^T[ACGT]AGC
440 GCTCAGC
BpmI 1210 CTCCAG CTGGAG(16/14)
810 CTGGAG
Bpu1102I 333 GCTCAGC GC^T[ACGT]AGC
440 GCTCAGC
BsaHI 1 GACGCC G[GA]^CG[CT]C
230 GGCGCC
BsgI 1105 CTGCAC GTGCAG(16/14)
1068 GTGCAG
BsmI 1500 GAATGC GAATGC(1/-1)
735 GCATTC
BspLU11I 641 ACATGT A^CATGT
1643 ACATGT
BspMI 148 ACCTGC ACCTGC(4/8)
365 GCAGGT
Bsu36I 119 CCTCAGG CC^T[ACGT]AGG
1474 CCTGAGG
Cac8I 395 GCTGGC GC[ACGT]^[ACGT]GC
445 GCTCGC
CelII 333 GCTCAGC GC^T[ACGT]AGC
440 GCTCAGC
CfoI 231 GCGC GCG^C
484 GCGC
DraI 1344 TTTAAA TTT^AAA
1390 TTTAAA
DsaI 294 CCATGG C^C[GA][CT]GG
543 CCATGG
Eco81I 119 CCTCAGG CC^T[ACGT]AGG
1474 CCTGAGG
EcoT22I 970 ATGCAT ATGCA^T
1370 ATGCAT
HhaI 231 GCGC GCG^C
484 GCGC
Hin1I 1 GACGCC G[GA]^CG[CT]C
230 GGCGCC
HinP1I 231 GCGC G^CGC
484 GCGC
HindIII 175 AAGCTT A^AGCTT
1381 AAGCTT
MaeII 468 ACGT A^CGT
1496 ACGT
NcoI 294 CCATGG C^CATGG
543 CCATGG
NsiI 970 ATGCAT ATGCA^T
1370 ATGCAT
NspI 641 ACATGT [GA]CATG^[CT]
1643 ACATGT
PflMI 256 CCAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
413 CCACCTCCTGG
SmlI 935 CTTAAG C^T[CT][GA]AG
1521 CTTGAG
SspI 1550 AATATT AAT^ATT
1568 AATATT
TfiI 88 GAATC G^A[AT]TC
1059 GAATC
Tsp45I 1169 GTGAC ^GT[GC]AC
1493 GTCAC
Van91I 256 CCAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
413 CCACCTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AfaI AluI AlwI AspHI AvaII
BanI BbvI BfaI BmyI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cfr13I DdeI DpnII
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI StyI Tru9I TseI
Tsp509I TspRI XcmI XhoII