################################## # NO CUT ENZYMES ( 107 enzymes) # ################################## AatII AccI AccII AccIII AclI AgeI AhdI Aor51HI ApaI AscI Asp700I AspEI AspI AvaI AviII BbvCI BcgI BclI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspHI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Cfr10I ClaI CpoI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO109I EcoO65I EcoRI EcoRV FbaI FseI FspI HincII HindII HpaI KspI MfeI MluI MroI MunI MvnI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI Psp1406I PstI PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### Acc65I 1227 GGTACC G^GTACC AflII 935 CTTAAG C^TTAAG Alw44I 1711 GTGCAC G^TGCAC AlwNI 189 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 1711 GTGCAC G^TGCAC AseI 1547 ATTAAT AT^TAAT AsnI 1547 ATTAAT AT^TAAT Asp718I 1227 GGTACC G^GTACC AvrII 1334 CCTAGG C^CTAGG BalI 546 TGGCCA TGG^CCA BanII 161 GAGCTC G[GA]GC[CT]^C BbeI 230 GGCGCC GGCGC^C BbrPI 1495 CACGTG CAC^GTG BbsI 565 GAAGAC GAAGAC(2/6) BciVI 753 GTATCC GTATCC(6/5) BcnI 560 CCGGG CC^[GC]GG BfrI 935 CTTAAG C^TTAAG BglI 1451 GCCACTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1534 AGATCT A^GATCT BlnI 1334 CCTAGG C^CTAGG BpuAI 565 GAAGAC GAAGAC(2/6) BsaAI 1495 CACGTG [CT]AC^GT[GA] BsaBI 1369 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC Bsp1407I 1640 TGTACA T^GTACA BsrBI 13 GAGCGG CCGCTC(-3/-3) BsrGI 1640 TGTACA T^GTACA EaeI 546 TGGCCA [CT]^GGCC[GA] EarI 794 CTCTTC CTCTTC(1/4) EcoNI 1474 CCTGAGGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 1240 CCCGC CCCGC(4/6) HaeII 230 GGCGCC [GA]GCGC^[CT] HapII 560 CCGG C^CGG HgaI 1 GACGC GACGC(5/10) HpaII 560 CCGG C^CGG KasI 230 GGCGCC G^GCGCC KpnI 1227 GGTACC GGTAC^C Ksp632I 794 CTCTTC CTCTTC(1/4) MamI 1369 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 546 TGGCCA TGG^CCA MscI 546 TGGCCA TGG^CCA MspA1I 216 CAGCTG C[AC]G^C[GT]G MspI 560 CCGG C^CGG NarI 230 GGCGCC GG^CGCC NciI 560 CCGGG CC^[GC]GG PmaCI 1495 CACGTG CAC^GTG PmlI 1495 CACGTG CAC^GTG PshAI 1519 GACTTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1547 ATTAAT AT^TAAT PvuII 216 CAGCTG CAG^CTG SacI 161 GAGCTC GAGCT^C SapI 793 GCTCTTC GCTCTTC(1/4) SfoI 230 GGCGCC GGC^GCC SspBI 1640 TGTACA T^GTACA TaqI 1666 TCGA T^CGA TthHB8I 1666 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AciI 1241 CCGC CCGC(-3/-1) 15 GCGG AcsI 691 AAATTT [GA]^AATT[CT] 1341 AAATTT AcyI 1 GACGCC G[GA]^CG[CT]C 230 GGCGCC AflIII 641 ACATGT A^C[GA][CT]GT 1643 ACATGT AocI 119 CCTCAGG CC^T[ACGT]AGG 1474 CCTGAGG ApoI 691 AAATTT [GA]^AATT[CT] 1341 AAATTT BamHI 1148 GGATCC G^GATCC 1560 GGATCC BlpI 333 GCTCAGC GC^T[ACGT]AGC 440 GCTCAGC BpmI 1210 CTCCAG CTGGAG(16/14) 810 CTGGAG Bpu1102I 333 GCTCAGC GC^T[ACGT]AGC 440 GCTCAGC BsaHI 1 GACGCC G[GA]^CG[CT]C 230 GGCGCC BsgI 1105 CTGCAC GTGCAG(16/14) 1068 GTGCAG BsmI 1500 GAATGC GAATGC(1/-1) 735 GCATTC BspLU11I 641 ACATGT A^CATGT 1643 ACATGT BspMI 148 ACCTGC ACCTGC(4/8) 365 GCAGGT Bsu36I 119 CCTCAGG CC^T[ACGT]AGG 1474 CCTGAGG Cac8I 395 GCTGGC GC[ACGT]^[ACGT]GC 445 GCTCGC CelII 333 GCTCAGC GC^T[ACGT]AGC 440 GCTCAGC CfoI 231 GCGC GCG^C 484 GCGC DraI 1344 TTTAAA TTT^AAA 1390 TTTAAA DsaI 294 CCATGG C^C[GA][CT]GG 543 CCATGG Eco81I 119 CCTCAGG CC^T[ACGT]AGG 1474 CCTGAGG EcoT22I 970 ATGCAT ATGCA^T 1370 ATGCAT HhaI 231 GCGC GCG^C 484 GCGC Hin1I 1 GACGCC G[GA]^CG[CT]C 230 GGCGCC HinP1I 231 GCGC G^CGC 484 GCGC HindIII 175 AAGCTT A^AGCTT 1381 AAGCTT MaeII 468 ACGT A^CGT 1496 ACGT NcoI 294 CCATGG C^CATGG 543 CCATGG NsiI 970 ATGCAT ATGCA^T 1370 ATGCAT NspI 641 ACATGT [GA]CATG^[CT] 1643 ACATGT PflMI 256 CCAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 413 CCACCTCCTGG SmlI 935 CTTAAG C^T[CT][GA]AG 1521 CTTGAG SspI 1550 AATATT AAT^ATT 1568 AATATT TfiI 88 GAATC G^A[AT]TC 1059 GAATC Tsp45I 1169 GTGAC ^GT[GC]AC 1493 GTCAC Van91I 256 CCAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 413 CCACCTCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AfaI AluI AlwI AspHI AvaII BanI BbvI BfaI BmyI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI StyI Tru9I TseI Tsp509I TspRI XcmI XhoII