################################## # NO CUT ENZYMES ( 100 enzymes) # ################################## AatII AccI AclI AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BalI BbrPI BbvCI BcgI BciVI BglI BlnI BsgI BsiWI BsmBI Bsp1407I BspDI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III EcoNI EcoO65I EcoRV EcoT22I FseI FspI HincII HindII HpaI MfeI MluI MluNI MscI MunI NdeI NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SalI SbfI SexAI SfiI SfuI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### Acc65I 911 GGTACC G^GTACC AccIII 437 TCCGGA T^CCGGA AcyI 135 GGCGCC G[GA]^CG[CT]C AflII 784 CTTAAG C^TTAAG AlwNI 955 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 765 CCTGAGG CC^T[ACGT]AGG ApaI 1523 GGGCCC GGGCC^C Asp718I 911 GGTACC G^GTACC AvaI 95 CCCGAG C^[CT]CG[GA]G BamHI 434 GGATCC G^GATCC BbeI 135 GGCGCC GGCGC^C BclI 971 TGATCA T^GATCA BfrI 784 CTTAAG C^TTAAG BglII 995 AGATCT A^GATCT BsaBI 1029 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 135 GGCGCC G[GA]^CG[CT]C BsaI 798 GAGACC GGTCTC(1/5) BsaWI 437 TCCGGA [AT]^CCGG[AT] BseAI 437 TCCGGA T^CCGGA BsmI 441 GAATGC GAATGC(1/-1) BsoBI 95 CCCGAG C^[CT]CG[GA]G BspEI 437 TCCGGA T^CCGGA BspHI 647 TCATGA T^CATGA Bsu36I 765 CCTGAGG CC^T[ACGT]AGG DraII 761 AGGACCT [GA]G^G[ACGT]CC[CT] Eco81I 765 CCTGAGG CC^T[ACGT]AGG EcoO109I 761 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRI 1358 GAATTC G^AATTC FbaI 971 TGATCA T^GATCA Hin1I 135 GGCGCC G[GA]^CG[CT]C HindIII 781 AAGCTT A^AGCTT KasI 135 GGCGCC G^GCGCC KpnI 911 GGTACC GGTAC^C MamI 1029 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 437 TCCGGA T^CCGGA NarI 135 GGCGCC GG^CGCC NcoI 608 CCATGG C^CATGG NotI 58 GCGGCCGC GC^GGCCGC PpuMI 761 AGGACCT [GA]G^G[AT]CC[CT] RcaI 647 TCATGA T^CATGA SacI 448 GAGCTC GAGCT^C ScaI 170 AGTACT AGT^ACT SfoI 135 GGCGCC GGC^GCC SgrAI 1075 CGCCGGCG C[GA]^CCGG[CT]G SmlI 784 CTTAAG C^T[CT][GA]AG StuI 1450 AGGCCT AGG^CCT TaqI 590 TCGA T^CGA TthHB8I 590 TCGA T^CGA XcmI 1047 CCAGCTGCAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcsI 1173 AAATTC [GA]^AATT[CT] 1358 GAATTC ApoI 1173 AAATTC [GA]^AATT[CT] 1358 GAATTC AspHI 448 GAGCTC G[AT]GC[AT]^C 1218 GAGCAC AvaII 76 GGACC G^G[AT]CC 762 GGACC BfaI 412 CTAG C^TAG 1193 CTAG BlpI 721 GCTCAGC GC^T[ACGT]AGC 960 GCTGAGC Bpu1102I 721 GCTCAGC GC^T[ACGT]AGC 960 GCTGAGC BsaAI 301 TACGTG [CT]AC^GT[GA] 1039 TACGTG BsiHKAI 448 GAGCTC G[AT]GC[AT]^C 1218 GAGCAC BspMI 840 ACCTGC ACCTGC(4/8) 1466 GCAGGT BsrBI 1271 CCGCTC CCGCTC(-3/-3) 430 GAGCGG BsrI 336 ACTGG ACTGG(1/-1) 619 ACTGG BstXI 929 CCATCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1557 CCACTTTCTTGG CelII 721 GCTCAGC GC^T[ACGT]AGC 960 GCTGAGC EcoT14I 608 CCATGG C^C[AT][AT]GG 1199 CCTTGG HgaI 47 GACGC GACGC(5/10) 771 GACGC MaeI 412 CTAG C^TAG 1193 CTAG MaeII 302 ACGT A^CGT 1040 ACGT MaeIII 789 GTCAC ^GT[ACGT]AC 1306 GTCAC MslI 1023 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1261 CATCTCTGTG StyI 608 CCATGG C^C[AT][AT]GG 1199 CCTTGG TfiI 625 GATTC G^A[AT]TC 940 GAATC Tsp45I 789 GTCAC ^GT[GC]AC 1306 GTCAC TspRI 980 CAGTG CA[GC]TG[ACGT][ACGT]^ 1221 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AccII AciI AfaI AluI AlwI BanI BanII BbsI BbvI BcnI BmyI BpmI BpuAI BsaJI BseRI BsiEI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DsaI EaeI EagI EarI EclXI Eco52I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I KspI MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PleI PstI PvuII RsaI SacII SapI Sau3AI Sau96I ScrFI SfaNI SfcI Tru9I TseI Tsp509I XhoII