##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII AccI AclI AflIII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BalI BbrPI BbvCI
BcgI BciVI BglI BlnI BsgI
BsiWI BsmBI Bsp1407I BspDI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III EcoNI EcoO65I EcoRV
EcoT22I FseI FspI HincII HindII
HpaI MfeI MluI MluNI MscI
MunI NdeI NheI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SalI SbfI SexAI SfiI SfuI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 911 GGTACC G^GTACC
AccIII 437 TCCGGA T^CCGGA
AcyI 135 GGCGCC G[GA]^CG[CT]C
AflII 784 CTTAAG C^TTAAG
AlwNI 955 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 765 CCTGAGG CC^T[ACGT]AGG
ApaI 1523 GGGCCC GGGCC^C
Asp718I 911 GGTACC G^GTACC
AvaI 95 CCCGAG C^[CT]CG[GA]G
BamHI 434 GGATCC G^GATCC
BbeI 135 GGCGCC GGCGC^C
BclI 971 TGATCA T^GATCA
BfrI 784 CTTAAG C^TTAAG
BglII 995 AGATCT A^GATCT
BsaBI 1029 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 135 GGCGCC G[GA]^CG[CT]C
BsaI 798 GAGACC GGTCTC(1/5)
BsaWI 437 TCCGGA [AT]^CCGG[AT]
BseAI 437 TCCGGA T^CCGGA
BsmI 441 GAATGC GAATGC(1/-1)
BsoBI 95 CCCGAG C^[CT]CG[GA]G
BspEI 437 TCCGGA T^CCGGA
BspHI 647 TCATGA T^CATGA
Bsu36I 765 CCTGAGG CC^T[ACGT]AGG
DraII 761 AGGACCT [GA]G^G[ACGT]CC[CT]
Eco81I 765 CCTGAGG CC^T[ACGT]AGG
EcoO109I 761 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 1358 GAATTC G^AATTC
FbaI 971 TGATCA T^GATCA
Hin1I 135 GGCGCC G[GA]^CG[CT]C
HindIII 781 AAGCTT A^AGCTT
KasI 135 GGCGCC G^GCGCC
KpnI 911 GGTACC GGTAC^C
MamI 1029 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 437 TCCGGA T^CCGGA
NarI 135 GGCGCC GG^CGCC
NcoI 608 CCATGG C^CATGG
NotI 58 GCGGCCGC GC^GGCCGC
PpuMI 761 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 647 TCATGA T^CATGA
SacI 448 GAGCTC GAGCT^C
ScaI 170 AGTACT AGT^ACT
SfoI 135 GGCGCC GGC^GCC
SgrAI 1075 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 784 CTTAAG C^T[CT][GA]AG
StuI 1450 AGGCCT AGG^CCT
TaqI 590 TCGA T^CGA
TthHB8I 590 TCGA T^CGA
XcmI 1047 CCAGCTGCAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 1173 AAATTC [GA]^AATT[CT]
1358 GAATTC
ApoI 1173 AAATTC [GA]^AATT[CT]
1358 GAATTC
AspHI 448 GAGCTC G[AT]GC[AT]^C
1218 GAGCAC
AvaII 76 GGACC G^G[AT]CC
762 GGACC
BfaI 412 CTAG C^TAG
1193 CTAG
BlpI 721 GCTCAGC GC^T[ACGT]AGC
960 GCTGAGC
Bpu1102I 721 GCTCAGC GC^T[ACGT]AGC
960 GCTGAGC
BsaAI 301 TACGTG [CT]AC^GT[GA]
1039 TACGTG
BsiHKAI 448 GAGCTC G[AT]GC[AT]^C
1218 GAGCAC
BspMI 840 ACCTGC ACCTGC(4/8)
1466 GCAGGT
BsrBI 1271 CCGCTC CCGCTC(-3/-3)
430 GAGCGG
BsrI 336 ACTGG ACTGG(1/-1)
619 ACTGG
BstXI 929 CCATCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1557 CCACTTTCTTGG
CelII 721 GCTCAGC GC^T[ACGT]AGC
960 GCTGAGC
EcoT14I 608 CCATGG C^C[AT][AT]GG
1199 CCTTGG
HgaI 47 GACGC GACGC(5/10)
771 GACGC
MaeI 412 CTAG C^TAG
1193 CTAG
MaeII 302 ACGT A^CGT
1040 ACGT
MaeIII 789 GTCAC ^GT[ACGT]AC
1306 GTCAC
MslI 1023 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1261 CATCTCTGTG
StyI 608 CCATGG C^C[AT][AT]GG
1199 CCTTGG
TfiI 625 GATTC G^A[AT]TC
940 GAATC
Tsp45I 789 GTCAC ^GT[GC]AC
1306 GTCAC
TspRI 980 CAGTG CA[GC]TG[ACGT][ACGT]^
1221 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
BanI BanII BbsI BbvI BcnI
BmyI BpmI BpuAI BsaJI BseRI
BsiEI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrFI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DsaI EaeI
EagI EarI EclXI Eco52I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I KspI MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
PleI PstI PvuII RsaI SacII
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI Tru9I TseI Tsp509I XhoII