################################## # NO CUT ENZYMES ( 111 enzymes) # ################################## AatII Acc65I AccIII AclI AflIII AgeI Alw44I ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BamHI BbeI BbrPI BcgI BciVI BclI BlnI BlpI Bpu1102I BsaAI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII Cfr10I ClaI CpoI DraI DrdI EagI EclXI Eco52I EcoO65I EcoRI EcoT22I FbaI FseI FspI HpaI KasI KpnI KspI MluI MroI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI SwaI TaqI Tth111I TthHB8I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccI 2414 GTAGAC GT^[AC][GT]AC AccII 13 CGCG CG^CG AcyI 1431 GACGCC G[GA]^CG[CT]C AflII 1162 CTTAAG C^TTAAG AhdI 1048 GACAGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 1166 AGCGCT AGC^GCT AspEI 1048 GACAGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 1441 TGGCCA TGG^CCA BcnI 1419 CCCGG CC^[GC]GG BfrI 1162 CTTAAG C^TTAAG BsaBI 1439 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1431 GACGCC G[GA]^CG[CT]C BsrBI 772 CCGCTC CCGCTC(-3/-3) BstAPI 1893 GCAGTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 13 CGCG CG^CG DraIII 748 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 1441 TGGCCA [CT]^GGCC[GA] Eam1105I 1048 GACAGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1166 AGCGCT AGC^GCT EcoRV 2225 GATATC GAT^ATC EcoT14I 1175 CCTTGG C^C[AT][AT]GG HaeII 1166 AGCGCT [GA]GCGC^[CT] HapII 1420 CCGG C^CGG HgaI 1431 GACGC GACGC(5/10) Hin1I 1431 GACGCC G[GA]^CG[CT]C HpaII 1420 CCGG C^CGG MaeII 28 ACGT A^CGT MamI 1439 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1441 TGGCCA TGG^CCA MscI 1441 TGGCCA TGG^CCA MspI 1420 CCGG C^CGG MvnI 13 CGCG CG^CG NciI 1419 CCCGG CC^[GC]GG PshAI 1500 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 1351 CAGCTG CAG^CTG SacI 2474 GAGCTC GAGCT^C ScaI 1072 AGTACT AGT^ACT SpeI 2105 ACTAGT A^CTAGT SspI 1182 AATATT AAT^ATT StyI 1175 CCTTGG C^C[AT][AT]GG TfiI 1923 GAATC G^A[AT]TC XcmI 1188 CCAGAAACAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcsI 876 AAATTC [GA]^AATT[CT] 2341 AAATTT AlwNI 790 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG 1250 CAGGCACTG ApoI 876 AAATTC [GA]^AATT[CT] 2341 AAATTT BanI 1531 GGTGCC G^G[CT][GA]CC 1973 GGCACC BglII 939 AGATCT A^GATCT 1041 AGATCT BsgI 1891 GTGCAG GTGCAG(16/14) 1900 GTGCAG BspMI 155 GCAGGT ACCTGC(4/8) 1902 GCAGGT BstXI 2352 CCAAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2662 CCACGGGGATGG BstYI 939 AGATCT [GA]^GATC[CT] 1041 AGATCT CfoI 868 GCGC GCG^C 1167 GCGC DsaI 593 CCACGG C^C[GA][CT]GG 2662 CCACGG HhaI 868 GCGC GCG^C 1167 GCGC HinP1I 868 GCGC G^CGC 1167 GCGC HincII 130 GTCAAC GT[CT]^[GA]AC 726 GTTGAC HindII 130 GTCAAC GT[CT]^[GA]AC 726 GTTGAC HindIII 1206 AAGCTT A^AGCTT 1880 AAGCTT MfeI 1104 CAATTG C^AATTG 2492 CAATTG MflI 939 AGATCT [GA]^GATC[CT] 1041 AGATCT MslI 211 CATCTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2663 CACGGGGATG MspA1I 1301 CAGCGG C[AC]G^C[GT]G 1351 CAGCTG MunI 1104 CAATTG C^AATTG 2492 CAATTG SapI 2531 GAAGAGC GCTCTTC(1/4) 445 GCTCTTC StuI 819 AGGCCT AGG^CCT 967 AGGCCT XbaI 1723 TCTAGA T^CTAGA 2335 TCTAGA XhoII 939 AGATCT [GA]^GATC[CT] 1041 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 80 enzymes) # ######################################### AciI AfaI AluI AlwI AocI AspHI AvaI AvaII BanII BbsI BbvCI BbvI BfaI BglI BmyI BpmI BpuAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI Bsu36I Cac8I Cfr13I DdeI DpnII DraII EarI Eco81I EcoNI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI Tru9I TseI Tsp45I Tsp509I TspRI Van91I