##################################
# NO CUT ENZYMES (  111 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflIII      
AgeI        Alw44I      ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspI        
AviII       AvrII       BamHI       BbeI        BbrPI       
BcgI        BciVI       BclI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     CelII       Cfr10I      ClaI        
CpoI        DraI        DrdI        EagI        EclXI       
Eco52I      EcoO65I     EcoRI       EcoT22I     FbaI        
FseI        FspI        HpaI        KasI        KpnI        
KspI        MluI        MroI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SphI        Sse8387I    SspBI       SwaI        TaqI        
Tth111I     TthHB8I     XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccI         2414  GTAGAC               GT^[AC][GT]AC
AccII          13  CGCG                 CG^CG
AcyI         1431  GACGCC               G[GA]^CG[CT]C
AflII        1162  CTTAAG               C^TTAAG
AhdI         1048  GACAGTTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      1166  AGCGCT               AGC^GCT
AspEI        1048  GACAGTTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI         1441  TGGCCA               TGG^CCA
BcnI         1419  CCCGG                CC^[GC]GG
BfrI         1162  CTTAAG               C^TTAAG
BsaBI        1439  GATGGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        1431  GACGCC               G[GA]^CG[CT]C
BsrBI         772  CCGCTC               CCGCTC(-3/-3)
BstAPI       1893  GCAGTGTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI          13  CGCG                 CG^CG
DraIII        748  CACTCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI         1441  TGGCCA               [CT]^GGCC[GA]
Eam1105I     1048  GACAGTTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1166  AGCGCT               AGC^GCT
EcoRV        2225  GATATC               GAT^ATC
EcoT14I      1175  CCTTGG               C^C[AT][AT]GG
HaeII        1166  AGCGCT               [GA]GCGC^[CT]
HapII        1420  CCGG                 C^CGG
HgaI         1431  GACGC                GACGC(5/10)
Hin1I        1431  GACGCC               G[GA]^CG[CT]C
HpaII        1420  CCGG                 C^CGG
MaeII          28  ACGT                 A^CGT
MamI         1439  GATGGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1441  TGGCCA               TGG^CCA
MscI         1441  TGGCCA               TGG^CCA
MspI         1420  CCGG                 C^CGG
MvnI           13  CGCG                 CG^CG
NciI         1419  CCCGG                CC^[GC]GG
PshAI        1500  GACTCAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII        1351  CAGCTG               CAG^CTG
SacI         2474  GAGCTC               GAGCT^C
ScaI         1072  AGTACT               AGT^ACT
SpeI         2105  ACTAGT               A^CTAGT
SspI         1182  AATATT               AAT^ATT
StyI         1175  CCTTGG               C^C[AT][AT]GG
TfiI         1923  GAATC                G^A[AT]TC
XcmI         1188  CCAGAAACAGGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AcsI          876  AAATTC               [GA]^AATT[CT]
             2341  AAATTT              
AlwNI         790  CAGCCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1250  CAGGCACTG           
ApoI          876  AAATTC               [GA]^AATT[CT]
             2341  AAATTT              
BanI         1531  GGTGCC               G^G[CT][GA]CC
             1973  GGCACC              
BglII         939  AGATCT               A^GATCT
             1041  AGATCT              
BsgI         1891  GTGCAG               GTGCAG(16/14)
             1900  GTGCAG              
BspMI         155  GCAGGT               ACCTGC(4/8)
             1902  GCAGGT              
BstXI        2352  CCAAAATGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2662  CCACGGGGATGG        
BstYI         939  AGATCT               [GA]^GATC[CT]
             1041  AGATCT              
CfoI          868  GCGC                 GCG^C
             1167  GCGC                
DsaI          593  CCACGG               C^C[GA][CT]GG
             2662  CCACGG              
HhaI          868  GCGC                 GCG^C
             1167  GCGC                
HinP1I        868  GCGC                 G^CGC
             1167  GCGC                
HincII        130  GTCAAC               GT[CT]^[GA]AC
              726  GTTGAC              
HindII        130  GTCAAC               GT[CT]^[GA]AC
              726  GTTGAC              
HindIII      1206  AAGCTT               A^AGCTT
             1880  AAGCTT              
MfeI         1104  CAATTG               C^AATTG
             2492  CAATTG              
MflI          939  AGATCT               [GA]^GATC[CT]
             1041  AGATCT              
MslI          211  CATCTGGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2663  CACGGGGATG          
MspA1I       1301  CAGCGG               C[AC]G^C[GT]G
             1351  CAGCTG              
MunI         1104  CAATTG               C^AATTG
             2492  CAATTG              
SapI         2531  GAAGAGC              GCTCTTC(1/4)
              445  GCTCTTC             
StuI          819  AGGCCT               AGG^CCT
              967  AGGCCT              
XbaI         1723  TCTAGA               T^CTAGA
             2335  TCTAGA              
XhoII         939  AGATCT               [GA]^GATC[CT]
             1041  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   80 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AocI        
AspHI       AvaI        AvaII       BanII       BbsI        
BbvCI       BbvI        BfaI        BglI        BmyI        
BpmI        BpuAI       BsaI        BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        BsoBI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Bsu36I      Cac8I       Cfr13I      
DdeI        DpnII       DraII       EarI        Eco81I      
EcoNI       EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HinfI       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MnlI        MseI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       NspI        PflMI       PleI        
PpuMI       PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       Van91I