##################################
# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI Alw44I ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BamHI BbeI BbrPI
BcgI BciVI BclI BlnI BlpI
Bpu1102I BsaAI BsaWI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I CelII Cfr10I ClaI
CpoI DraI DrdI EagI EclXI
Eco52I EcoO65I EcoRI EcoT22I FbaI
FseI FspI HpaI KasI KpnI
KspI MluI MroI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SspBI SwaI TaqI
Tth111I TthHB8I XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 2414 GTAGAC GT^[AC][GT]AC
AccII 13 CGCG CG^CG
AcyI 1431 GACGCC G[GA]^CG[CT]C
AflII 1162 CTTAAG C^TTAAG
AhdI 1048 GACAGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1166 AGCGCT AGC^GCT
AspEI 1048 GACAGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1441 TGGCCA TGG^CCA
BcnI 1419 CCCGG CC^[GC]GG
BfrI 1162 CTTAAG C^TTAAG
BsaBI 1439 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1431 GACGCC G[GA]^CG[CT]C
BsrBI 772 CCGCTC CCGCTC(-3/-3)
BstAPI 1893 GCAGTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 13 CGCG CG^CG
DraIII 748 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1441 TGGCCA [CT]^GGCC[GA]
Eam1105I 1048 GACAGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1166 AGCGCT AGC^GCT
EcoRV 2225 GATATC GAT^ATC
EcoT14I 1175 CCTTGG C^C[AT][AT]GG
HaeII 1166 AGCGCT [GA]GCGC^[CT]
HapII 1420 CCGG C^CGG
HgaI 1431 GACGC GACGC(5/10)
Hin1I 1431 GACGCC G[GA]^CG[CT]C
HpaII 1420 CCGG C^CGG
MaeII 28 ACGT A^CGT
MamI 1439 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1441 TGGCCA TGG^CCA
MscI 1441 TGGCCA TGG^CCA
MspI 1420 CCGG C^CGG
MvnI 13 CGCG CG^CG
NciI 1419 CCCGG CC^[GC]GG
PshAI 1500 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1351 CAGCTG CAG^CTG
SacI 2474 GAGCTC GAGCT^C
ScaI 1072 AGTACT AGT^ACT
SpeI 2105 ACTAGT A^CTAGT
SspI 1182 AATATT AAT^ATT
StyI 1175 CCTTGG C^C[AT][AT]GG
TfiI 1923 GAATC G^A[AT]TC
XcmI 1188 CCAGAAACAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 876 AAATTC [GA]^AATT[CT]
2341 AAATTT
AlwNI 790 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
1250 CAGGCACTG
ApoI 876 AAATTC [GA]^AATT[CT]
2341 AAATTT
BanI 1531 GGTGCC G^G[CT][GA]CC
1973 GGCACC
BglII 939 AGATCT A^GATCT
1041 AGATCT
BsgI 1891 GTGCAG GTGCAG(16/14)
1900 GTGCAG
BspMI 155 GCAGGT ACCTGC(4/8)
1902 GCAGGT
BstXI 2352 CCAAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2662 CCACGGGGATGG
BstYI 939 AGATCT [GA]^GATC[CT]
1041 AGATCT
CfoI 868 GCGC GCG^C
1167 GCGC
DsaI 593 CCACGG C^C[GA][CT]GG
2662 CCACGG
HhaI 868 GCGC GCG^C
1167 GCGC
HinP1I 868 GCGC G^CGC
1167 GCGC
HincII 130 GTCAAC GT[CT]^[GA]AC
726 GTTGAC
HindII 130 GTCAAC GT[CT]^[GA]AC
726 GTTGAC
HindIII 1206 AAGCTT A^AGCTT
1880 AAGCTT
MfeI 1104 CAATTG C^AATTG
2492 CAATTG
MflI 939 AGATCT [GA]^GATC[CT]
1041 AGATCT
MslI 211 CATCTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2663 CACGGGGATG
MspA1I 1301 CAGCGG C[AC]G^C[GT]G
1351 CAGCTG
MunI 1104 CAATTG C^AATTG
2492 CAATTG
SapI 2531 GAAGAGC GCTCTTC(1/4)
445 GCTCTTC
StuI 819 AGGCCT AGG^CCT
967 AGGCCT
XbaI 1723 TCTAGA T^CTAGA
2335 TCTAGA
XhoII 939 AGATCT [GA]^GATC[CT]
1041 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AfaI AluI AlwI AocI
AspHI AvaI AvaII BanII BbsI
BbvCI BbvI BfaI BglI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI Bsu36I Cac8I Cfr13I
DdeI DpnII DraII EarI Eco81I
EcoNI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I