##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI AhdI AocI Aor51HI AscI
Asp700I Asp718I AspEI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BcgI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsiEI BsiWI
BsmBI BspHI BspLU11I BsrBI BsrDI
BssHII BssSI Bst1107I BstBI BstZ17I
Bsu36I CelII CpoI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KasI KpnI MfeI MluI MluNI
MscI MunI NarI NcoI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI Psp1406I PvuI RcaI RsrII
SacI SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I StuI XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccI 1139 GTCTAC GT^[AC][GT]AC
AccII 1123 CGCG CG^CG
AccIII 2000 TCCGGA T^CCGGA
AcyI 1290 GACGCC G[GA]^CG[CT]C
Alw44I 1401 GTGCAC G^TGCAC
ApaI 92 GGGCCC GGGCC^C
ApaLI 1401 GTGCAC G^TGCAC
AseI 1380 ATTAAT AT^TAAT
AsnI 1380 ATTAAT AT^TAAT
AspI 2085 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 1628 GGTGCC G^G[CT][GA]CC
BciVI 1671 GGATAC GTATCC(6/5)
BglI 1111 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 568 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1290 GACGCC G[GA]^CG[CT]C
BsaWI 2000 TCCGGA [AT]^CCGG[AT]
BseAI 2000 TCCGGA T^CCGGA
BsgI 1798 CTGCAC GTGCAG(16/14)
BspDI 319 ATCGAT AT^CGAT
BspEI 2000 TCCGGA T^CCGGA
BstAPI 870 GCAGATGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1299 GGTTACC G^GT[ACGT]ACC
BstPI 1299 GGTTACC G^GT[ACGT]ACC
BstUI 1123 CGCG CG^CG
BstXI 1304 CCAAACACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 319 ATCGAT AT^CGAT
DraII 539 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoNI 990 CCTCGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 539 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1299 GGTTACC G^GT[ACGT]ACC
EcoRI 1575 GAATTC G^AATTC
EcoRV 2053 GATATC GAT^ATC
HaeII 1347 AGCGCC [GA]GCGC^[CT]
HgaI 1290 GACGC GACGC(5/10)
Hin1I 1290 GACGCC G[GA]^CG[CT]C
KspI 1122 CCGCGG CCGC^GG
MamI 568 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2000 TCCGGA T^CCGGA
MvnI 1123 CGCG CG^CG
NdeI 351 CATATG CA^TATG
PflFI 2085 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 366 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 539 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 1380 ATTAAT AT^TAAT
SacII 1122 CCGCGG CCGC^GG
ScaI 407 AGTACT AGT^ACT
SmaI 473 CCCGGG CCC^GGG
SmlI 1711 CTTGAG C^T[CT][GA]AG
SspI 390 AATATT AAT^ATT
SwaI 2190 ATTTAAAT ATTT^AAAT
Tth111I 2085 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 366 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 473 CCCGGG C^CCGGG
XmaI 473 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BsaI 469 GAGACC GGTCTC(1/5)
1980 GAGACC
BsmI 279 GAATGC GAATGC(1/-1)
854 GCATTC
BspMI 1521 ACCTGC ACCTGC(4/8)
879 GCAGGT
BsrFI 44 GCCGGC [GA]^CCGG[CT]
1131 GCCGGC
BstYI 1647 GGATCT [GA]^GATC[CT]
2168 GGATCT
Cfr10I 44 GCCGGC [GA]^CCGG[CT]
1131 GCCGGC
DraI 2191 TTTAAA TTT^AAA
2229 TTTAAA
DsaI 956 CCGTGG C^C[GA][CT]GG
1122 CCGCGG
MflI 1647 GGATCT [GA]^GATC[CT]
2168 GGATCT
NaeI 44 GCCGGC GCC^GGC
1131 GCCGGC
NgoMIV 44 GCCGGC G^CCGGC
1131 GCCGGC
PvuII 948 CAGCTG CAG^CTG
1972 CAGCTG
SapI 1429 GAAGAGC GCTCTTC(1/4)
2361 GCTCTTC
TaqI 320 TCGA T^CGA
992 TCGA
TthHB8I 320 TCGA T^CGA
992 TCGA
XcmI 234 CCATGTCCTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2163 CCATTGGATCTATGG
XhoII 1647 GGATCT [GA]^GATC[CT]
2168 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BsrGI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII EarI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PshAI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspBI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI