################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AatII Acc65I AclI AflII AflIII AgeI AhdI AocI Aor51HI AscI Asp700I Asp718I AspEI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsiEI BsiWI BsmBI BspHI BspLU11I BsrBI BsrDI BssHII BssSI Bst1107I BstBI BstZ17I Bsu36I CelII CpoI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI MfeI MluI MluNI MscI MunI NarI NcoI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI Psp1406I PvuI RcaI RsrII SacI SalI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I StuI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccI 1139 GTCTAC GT^[AC][GT]AC AccII 1123 CGCG CG^CG AccIII 2000 TCCGGA T^CCGGA AcyI 1290 GACGCC G[GA]^CG[CT]C Alw44I 1401 GTGCAC G^TGCAC ApaI 92 GGGCCC GGGCC^C ApaLI 1401 GTGCAC G^TGCAC AseI 1380 ATTAAT AT^TAAT AsnI 1380 ATTAAT AT^TAAT AspI 2085 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 1628 GGTGCC G^G[CT][GA]CC BciVI 1671 GGATAC GTATCC(6/5) BglI 1111 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 568 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1290 GACGCC G[GA]^CG[CT]C BsaWI 2000 TCCGGA [AT]^CCGG[AT] BseAI 2000 TCCGGA T^CCGGA BsgI 1798 CTGCAC GTGCAG(16/14) BspDI 319 ATCGAT AT^CGAT BspEI 2000 TCCGGA T^CCGGA BstAPI 870 GCAGATGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1299 GGTTACC G^GT[ACGT]ACC BstPI 1299 GGTTACC G^GT[ACGT]ACC BstUI 1123 CGCG CG^CG BstXI 1304 CCAAACACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ClaI 319 ATCGAT AT^CGAT DraII 539 GGGACCT [GA]G^G[ACGT]CC[CT] EcoNI 990 CCTCGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 539 GGGACCT [GA]G^G[ACGT]CC[CT] EcoO65I 1299 GGTTACC G^GT[ACGT]ACC EcoRI 1575 GAATTC G^AATTC EcoRV 2053 GATATC GAT^ATC HaeII 1347 AGCGCC [GA]GCGC^[CT] HgaI 1290 GACGC GACGC(5/10) Hin1I 1290 GACGCC G[GA]^CG[CT]C KspI 1122 CCGCGG CCGC^GG MamI 568 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 2000 TCCGGA T^CCGGA MvnI 1123 CGCG CG^CG NdeI 351 CATATG CA^TATG PflFI 2085 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 366 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 539 GGGACCT [GA]G^G[AT]CC[CT] PshBI 1380 ATTAAT AT^TAAT SacII 1122 CCGCGG CCGC^GG ScaI 407 AGTACT AGT^ACT SmaI 473 CCCGGG CCC^GGG SmlI 1711 CTTGAG C^T[CT][GA]AG SspI 390 AATATT AAT^ATT SwaI 2190 ATTTAAAT ATTT^AAAT Tth111I 2085 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 366 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 473 CCCGGG C^CCGGG XmaI 473 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### BsaI 469 GAGACC GGTCTC(1/5) 1980 GAGACC BsmI 279 GAATGC GAATGC(1/-1) 854 GCATTC BspMI 1521 ACCTGC ACCTGC(4/8) 879 GCAGGT BsrFI 44 GCCGGC [GA]^CCGG[CT] 1131 GCCGGC BstYI 1647 GGATCT [GA]^GATC[CT] 2168 GGATCT Cfr10I 44 GCCGGC [GA]^CCGG[CT] 1131 GCCGGC DraI 2191 TTTAAA TTT^AAA 2229 TTTAAA DsaI 956 CCGTGG C^C[GA][CT]GG 1122 CCGCGG MflI 1647 GGATCT [GA]^GATC[CT] 2168 GGATCT NaeI 44 GCCGGC GCC^GGC 1131 GCCGGC NgoMIV 44 GCCGGC G^CCGGC 1131 GCCGGC PvuII 948 CAGCTG CAG^CTG 1972 CAGCTG SapI 1429 GAAGAGC GCTCTTC(1/4) 2361 GCTCTTC TaqI 320 TCGA T^CGA 992 TCGA TthHB8I 320 TCGA T^CGA 992 TCGA XcmI 234 CCATGTCCTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2163 CCATTGGATCTATGG XhoII 1647 GGATCT [GA]^GATC[CT] 2168 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BsrGI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PshAI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspBI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI