##################################
# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI AlwNI ApaI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BbeI BclI BglI BglII
BlnI BsaBI BsaHI BsaWI BseAI
BsgI BsiEI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BsrDI BsrFI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Cfr10I
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco52I EcoO65I EcoRV FbaI
FseI FspI Hin1I HincII HindII
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NcoI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI Tth111I Van91I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 945 GTAGAC GT^[AC][GT]AC
AflII 1875 CTTAAG C^TTAAG
AflIII 955 ACATGT A^C[GA][CT]GT
Alw44I 1323 GTGCAC G^TGCAC
AocI 357 CCTGAGG CC^T[ACGT]AGG
ApaLI 1323 GTGCAC G^TGCAC
Asp700I 49 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 1643 GGCACC G^G[CT][GA]CC
BbrPI 1965 CACGTG CAC^GTG
BbvCI 1788 CCTCAGC CCTCAGC(-5/-2)
BcgI 136 GCATCTGTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 12 CCCGG CC^[GC]GG
BfrI 1875 CTTAAG C^TTAAG
BsaAI 1965 CACGTG [CT]AC^GT[GA]
BseRI 999 CTCCTC GAGGAG(10/8)
BsiWI 1339 CGTACG C^GTACG
BspLU11I 955 ACATGT A^CATGT
BspMI 1300 ACCTGC ACCTGC(4/8)
BssSI 1587 CTCGTG CACGAG(-5/-1)
BstAPI 879 GCAGCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 357 CCTGAGG CC^T[ACGT]AGG
DrdI 756 GACCCACCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 357 CCTGAGG CC^T[ACGT]AGG
EcoNI 2012 CCTATATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 858 GAATTC G^AATTC
EcoT22I 134 ATGCAT ATGCA^T
HapII 13 CCGG C^CGG
HgaI 1517 GACGC GACGC(5/10)
HindIII 1872 AAGCTT A^AGCTT
HpaII 13 CCGG C^CGG
MspI 13 CCGG C^CGG
NciI 12 CCCGG CC^[GC]GG
NdeI 234 CATATG CA^TATG
NsiI 134 ATGCAT ATGCA^T
PmaCI 1965 CACGTG CAC^GTG
PmlI 1965 CACGTG CAC^GTG
PstI 1302 CTGCAG CTGCA^G
SapI 269 GAAGAGC GCTCTTC(1/4)
SmlI 1875 CTTAAG C^T[CT][GA]AG
SphI 1454 GCATGC GCATG^C
StuI 1368 AGGCCT AGG^CCT
SwaI 474 ATTTAAAT ATTT^AAAT
XcmI 1418 CCATGTTAGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 49 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwI 1200 GATCC GGATC(4/5)
1402 GGATC
Aor51HI 1563 AGCGCT AGC^GCT
1759 AGCGCT
BbsI 418 GTCTTC GAAGAC(2/6)
657 GTCTTC
BpuAI 418 GTCTTC GAAGAC(2/6)
657 GTCTTC
BsaI 594 GGTCTC GGTCTC(1/5)
1443 GGTCTC
BsmFI 754 GGGAC GGGAC(10/14)
1768 GTCCC
BsmI 790 GAATGC GAATGC(1/-1)
2088 GCATTC
BstYI 1199 AGATCC [GA]^GATC[CT]
1402 GGATCT
DraI 475 TTTAAA TTT^AAA
2187 TTTAAA
DsaI 750 CCGTGG C^C[GA][CT]GG
1885 CCGTGG
EaeI 1024 TGGCCG [CT]^GGCC[GA]
1463 TGGCCG
Eco47III 1563 AGCGCT AGC^GCT
1759 AGCGCT
HphI 1911 GGTGA GGTGA(8/7)
354 TCACC
MaeII 1327 ACGT A^CGT
1966 ACGT
MflI 1199 AGATCC [GA]^GATC[CT]
1402 GGATCT
NspI 955 ACATGT [GA]CATG^[CT]
1454 GCATGC
SacI 614 GAGCTC GAGCT^C
1617 GAGCTC
SfcI 853 CTACAG C^T[GA][CT]AG
1302 CTGCAG
SspI 699 AATATT AAT^ATT
1144 AATATT
TaqI 812 TCGA T^CGA
2050 TCGA
Tsp45I 1192 GTCAC ^GT[GC]AC
1632 GTGAC
TthHB8I 812 TCGA T^CGA
2050 TCGA
XhoII 1199 AGATCC [GA]^GATC[CT]
1402 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI AvaII BanII BbvI BciVI
BfaI BlpI BmyI BpmI Bpu1102I
BsaJI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BsrBI BsrI BssKI BstF5I
BstNI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HhaI HinP1I HinfI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MslI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TfiI
Tru9I TseI Tsp509I TspRI