##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI AscI Asp700I Asp718I AspEI
AspI AviII BalI BamHI BbeI
BbrPI BclI BfrI BglII BlpI
Bpu1102I BsaBI BsaI BsiWI BsmI
BspDI BspHI BspMI BssSI BstEII
BstPI BstXI BstYI Bsu36I CelII
ClaI DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoO65I EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HpaI KasI KpnI MamI MfeI
MflI MluI MluNI MscI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RcaI SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SmlI SpeI
Sse8387I SwaI Tth111I Van91I XbaI
XhoI XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccIII 1376 TCCGGA T^CCGGA
AcsI 69 GAATTC [GA]^AATT[CT]
AcyI 237 GACGCC G[GA]^CG[CT]C
AlwI 591 GGATC GGATC(4/5)
ApoI 69 GAATTC [GA]^AATT[CT]
AseI 1519 ATTAAT AT^TAAT
AsnI 1519 ATTAAT AT^TAAT
AvrII 1146 CCTAGG C^CTAGG
BanI 789 GGTGCC G^G[CT][GA]CC
BbvCI 765 CCTCAGC CCTCAGC(-5/-2)
BcgI 201 CGACAAGCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 405 GGATAC GTATCC(6/5)
BglI 1531 GCCATTAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1146 CCTAGG C^CTAGG
BsaHI 237 GACGCC G[GA]^CG[CT]C
BseAI 1376 TCCGGA T^CCGGA
BsiEI 1504 CGGCCG CG[GA][CT]^CG
BspEI 1376 TCCGGA T^CCGGA
BsrDI 131 GCAATG GCAATG(2/0)
BsrFI 1371 GCCGGT [GA]^CCGG[CT]
BssHII 1329 GCGCGC G^CGCGC
Bst1107I 427 GTATAC GTA^TAC
BstBI 859 TTCGAA TT^CGAA
BstZ17I 427 GTATAC GTA^TAC
Cfr10I 1371 GCCGGT [GA]^CCGG[CT]
CpoI 1373 CGGTCCG CG^G[AT]CCG
DpnII 592 GATC ^GATC
EagI 1504 CGGCCG C^GGCCG
EarI 1294 GAAGAG CTCTTC(1/4)
EclXI 1504 CGGCCG C^GGCCG
Eco52I 1504 CGGCCG C^GGCCG
EcoNI 393 CCTTGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 69 GAATTC G^AATTC
Hin1I 237 GACGCC G[GA]^CG[CT]C
HindIII 1418 AAGCTT A^AGCTT
Ksp632I 1294 GAAGAG CTCTTC(1/4)
KspI 5 CCGCGG CCGC^GG
MaeIII 318 GTCAC ^GT[ACGT]AC
MboI 592 GATC ^GATC
MroI 1376 TCCGGA T^CCGGA
NcoI 734 CCATGG C^CATGG
NdeII 592 GATC ^GATC
NspV 859 TTCGAA TT^CGAA
PshAI 898 GACATTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1519 ATTAAT AT^TAAT
PvuII 277 CAGCTG CAG^CTG
RsrII 1373 CGGTCCG CG^G[AT]CCG
SacII 5 CCGCGG CCGC^GG
Sau3AI 592 GATC ^GATC
SfaNI 938 GCATC GCATC(5/9)
SfuI 859 TTCGAA TT^CGAA
SnaBI 1437 TACGTA TAC^GTA
SspI 947 AATATT AAT^ATT
StuI 1355 AGGCCT AGG^CCT
TfiI 376 GAATC G^A[AT]TC
Tsp45I 318 GTCAC ^GT[GC]AC
XcmI 776 CCATCAGTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 427 GTATAC GT^[AC][GT]AC
1223 GTAGAC
ApaI 480 GGGCCC GGGCC^C
1203 GGGCCC
AspHI 109 GAGCTC G[AT]GC[AT]^C
292 GAGCTC
AvaI 644 CCCGAG C^[CT]CG[GA]G
710 CCCGAG
BbsI 164 GAAGAC GAAGAC(2/6)
1407 GAAGAC
BcnI 42 CCGGG CC^[GC]GG
1349 CCCGG
BfaI 935 CTAG C^TAG
1147 CTAG
BpmI 75 CTCCAG CTGGAG(16/14)
1074 CTCCAG
BpuAI 164 GAAGAC GAAGAC(2/6)
1407 GAAGAC
BsaAI 979 TACGTG [CT]AC^GT[GA]
1437 TACGTA
BsaWI 1376 TCCGGA [AT]^CCGG[AT]
1384 ACCGGA
BsiHKAI 109 GAGCTC G[AT]GC[AT]^C
292 GAGCTC
BsmBI 235 GAGACG CGTCTC(1/5)
1500 GAGACG
BsoBI 644 CCCGAG C^[CT]CG[GA]G
710 CCCGAG
Bsp1407I 746 TGTACA T^GTACA
1034 TGTACA
BsrBI 793 CCGCTC CCGCTC(-3/-3)
1581 CCGCTC
BsrGI 746 TGTACA T^GTACA
1034 TGTACA
BstAPI 1353 GCAGGCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1362 GCAGGGCATGC
BstF5I 1038 CATCC GGATG(2/0)
1592 CATCC
BstNI 651 CCTGG CC^[AT]GG
1199 CCTGG
EaeI 29 CGGCCA [CT]^GGCC[GA]
1504 CGGCCG
EcoRII 651 CCTGG ^CC[AT]GG
1199 CCTGG
EcoT14I 734 CCATGG C^C[AT][AT]GG
1146 CCTAGG
FokI 1038 CATCC GGATG(9/13)
1592 CATCC
HgaI 237 GACGC GACGC(5/10)
1551 GACGC
HphI 466 GGTGA GGTGA(8/7)
719 TCACC
MaeI 935 CTAG C^TAG
1147 CTAG
MspA1I 5 CCGCGG C[AC]G^C[GT]G
277 CAGCTG
MvaI 651 CCTGG CC^[AT]GG
1199 CCTGG
NciI 42 CCGGG CC^[GC]GG
1349 CCCGG
SacI 109 GAGCTC GAGCT^C
292 GAGCTC
SphI 207 GCATGC GCATG^C
1367 GCATGC
SspBI 746 TGTACA T^GTACA
1034 TGTACA
StyI 734 CCATGG C^C[AT][AT]GG
1146 CCTAGG
TaqI 594 TCGA T^CGA
860 TCGA
TthHB8I 594 TCGA T^CGA
860 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AvaII BanII BbvI BmyI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BspLU11I BsrI BssKI
BstUI Cac8I CfoI Cfr13I DdeI
DraII DsaI EcoO109I FauI Fnu4HI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MaeII MboII
MnlI MseI MslI MspI MvnI
MwoI NlaIII NlaIV NspI PleI
PpuMI PstI RsaI Sau96I ScrFI
SfcI Tru9I TseI Tsp509I TspRI