##################################
# NO CUT ENZYMES (  137 enzymes) #
##################################

Acc65I      AccI        AccII       AccIII      AclI        
AgeI        AhdI        Aor51HI     ApaI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BalI        BbeI        
BbrPI       BcgI        BciVI       BclI        BcnI        
BglI        BlnI        BlpI        Bpu1102I    BsaBI       
BsaJI       BsaWI       BseAI       BsiEI       BsiWI       
BsmI        Bsp1407I    BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstEII      
BstPI       BstUI       BstXI       BstZ17I     CelII       
CfoI        Cfr10I      CpoI        DraI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRI       EcoT14I     
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HapII       HgaI        HhaI        HinP1I      
HpaII       KasI        KpnI        KspI        MamI        
MluI        MluNI       MroI        MscI        MslI        
MspI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NotI        NruI        
NsiI        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        Tth111I     XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AatII          98  GACGTC               GACGT^C
AciI         1518  GCGG                 CCGC(-3/-1)
AcsI         1768  AAATTC               [GA]^AATT[CT]
AcyI           98  GACGTC               G[GA]^CG[CT]C
AflII         786  CTTAAG               C^TTAAG
AflIII         40  ACACGT               A^C[GA][CT]GT
Alw44I       1537  GTGCAC               G^TGCAC
AlwNI         947  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1035  CCTCAGG              CC^T[ACGT]AGG
ApaLI        1537  GTGCAC               G^TGCAC
ApoI         1768  AAATTC               [GA]^AATT[CT]
AvaI         1074  CTCGGG               C^[CT]CG[GA]G
BamHI        1461  GGATCC               G^GATCC
BanI         1091  GGTGCC               G^G[CT][GA]CC
BbvCI         270  GCTGAGG              CCTCAGC(-5/-2)
BfrI          786  CTTAAG               C^TTAAG
BglII        1850  AGATCT               A^GATCT
BsaAI         181  CACGTA               [CT]AC^GT[GA]
BsaHI          98  GACGTC               G[GA]^CG[CT]C
BsmBI         683  CGTCTC               CGTCTC(1/5)
BsmFI        1067  GTCCC                GGGAC(10/14)
BsoBI        1074  CTCGGG               C^[CT]CG[GA]G
BspDI         741  ATCGAT               AT^CGAT
Bsu36I       1035  CCTCAGG              CC^T[ACGT]AGG
ClaI          741  ATCGAT               AT^CGAT
DraIII       1162  CACAGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1058  GACTTCCAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1035  CCTCAGG              CC^T[ACGT]AGG
EcoRV         175  GATATC               GAT^ATC
Hin1I          98  GACGTC               G[GA]^CG[CT]C
HincII       1017  GTTAAC               GT[CT]^[GA]AC
HindII       1017  GTTAAC               GT[CT]^[GA]AC
HpaI         1017  GTTAAC               GTT^AAC
MfeI          156  CAATTG               C^AATTG
MunI          156  CAATTG               C^AATTG
NheI          612  GCTAGC               G^CTAGC
PflMI        1161  CCACAGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1065  AGGTCCC              [GA]G^G[AT]CC[CT]
PstI         1351  CTGCAG               CTGCA^G
SapI         1637  GCTCTTC              GCTCTTC(1/4)
SphI          379  GCATGC               GCATG^C
Tsp45I        535  GTGAC                ^GT[GC]AC
Van91I       1161  CCACAGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1070  CCAGCTCGGGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AfaI          248  GTAC                 GT^AC
             1175  GTAC                
AvaII         495  GGACC                G^G[AT]CC
             1066  GGTCC               
BsaI          309  GAGACC               GGTCTC(1/5)
             1406  GAGACC              
BsgI          151  CTGCAC               GTGCAG(16/14)
              549  CTGCAC              
BstBI         116  TTCGAA               TT^CGAA
             1358  TTCGAA              
HindIII       163  AAGCTT               A^AGCTT
              556  AAGCTT              
MspA1I        196  CAGCTG               C[AC]G^C[GT]G
              950  CAGCTG              
NspI          379  GCATGC               [GA]CATG^[CT]
              875  ACATGC              
NspV          116  TTCGAA               TT^CGAA
             1358  TTCGAA              
PvuII         196  CAGCTG               CAG^CTG
              950  CAGCTG              
RsaI          248  GTAC                 GT^AC
             1175  GTAC                
SfuI          116  TTCGAA               TT^CGAA
             1358  TTCGAA              


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AluI        AlwI        AspHI       BanII       BbsI        
BbvI        BfaI        BmyI        BpmI        BpuAI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       EarI        EcoO109I    EcoRII      Fnu4HI      
FokI        HaeIII      HinfI       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       PleI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        TaqI        TfiI        Tru9I       
TseI        Tsp509I     TspRI       TthHB8I     XhoII