################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BbeI BbrPI BcgI BciVI BclI BcnI BglI BlnI BlpI Bpu1102I BsaBI BsaJI BsaWI BseAI BsiEI BsiWI BsmI Bsp1407I BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I CelII CfoI Cfr10I CpoI DraI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI HinP1I HpaII KasI KpnI KspI MamI MluI MluNI MroI MscI MslI MspI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AatII 98 GACGTC GACGT^C AciI 1518 GCGG CCGC(-3/-1) AcsI 1768 AAATTC [GA]^AATT[CT] AcyI 98 GACGTC G[GA]^CG[CT]C AflII 786 CTTAAG C^TTAAG AflIII 40 ACACGT A^C[GA][CT]GT Alw44I 1537 GTGCAC G^TGCAC AlwNI 947 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1035 CCTCAGG CC^T[ACGT]AGG ApaLI 1537 GTGCAC G^TGCAC ApoI 1768 AAATTC [GA]^AATT[CT] AvaI 1074 CTCGGG C^[CT]CG[GA]G BamHI 1461 GGATCC G^GATCC BanI 1091 GGTGCC G^G[CT][GA]CC BbvCI 270 GCTGAGG CCTCAGC(-5/-2) BfrI 786 CTTAAG C^TTAAG BglII 1850 AGATCT A^GATCT BsaAI 181 CACGTA [CT]AC^GT[GA] BsaHI 98 GACGTC G[GA]^CG[CT]C BsmBI 683 CGTCTC CGTCTC(1/5) BsmFI 1067 GTCCC GGGAC(10/14) BsoBI 1074 CTCGGG C^[CT]CG[GA]G BspDI 741 ATCGAT AT^CGAT Bsu36I 1035 CCTCAGG CC^T[ACGT]AGG ClaI 741 ATCGAT AT^CGAT DraIII 1162 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1058 GACTTCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1035 CCTCAGG CC^T[ACGT]AGG EcoRV 175 GATATC GAT^ATC Hin1I 98 GACGTC G[GA]^CG[CT]C HincII 1017 GTTAAC GT[CT]^[GA]AC HindII 1017 GTTAAC GT[CT]^[GA]AC HpaI 1017 GTTAAC GTT^AAC MfeI 156 CAATTG C^AATTG MunI 156 CAATTG C^AATTG NheI 612 GCTAGC G^CTAGC PflMI 1161 CCACAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1065 AGGTCCC [GA]G^G[AT]CC[CT] PstI 1351 CTGCAG CTGCA^G SapI 1637 GCTCTTC GCTCTTC(1/4) SphI 379 GCATGC GCATG^C Tsp45I 535 GTGAC ^GT[GC]AC Van91I 1161 CCACAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1070 CCAGCTCGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AfaI 248 GTAC GT^AC 1175 GTAC AvaII 495 GGACC G^G[AT]CC 1066 GGTCC BsaI 309 GAGACC GGTCTC(1/5) 1406 GAGACC BsgI 151 CTGCAC GTGCAG(16/14) 549 CTGCAC BstBI 116 TTCGAA TT^CGAA 1358 TTCGAA HindIII 163 AAGCTT A^AGCTT 556 AAGCTT MspA1I 196 CAGCTG C[AC]G^C[GT]G 950 CAGCTG NspI 379 GCATGC [GA]CATG^[CT] 875 ACATGC NspV 116 TTCGAA TT^CGAA 1358 TTCGAA PvuII 196 CAGCTG CAG^CTG 950 CAGCTG RsaI 248 GTAC GT^AC 1175 GTAC SfuI 116 TTCGAA TT^CGAA 1358 TTCGAA ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AluI AlwI AspHI BanII BbsI BbvI BfaI BmyI BpmI BpuAI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII NlaIV PleI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII