##################################
# NO CUT ENZYMES ( 137 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AgeI AhdI Aor51HI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BbeI
BbrPI BcgI BciVI BclI BcnI
BglI BlnI BlpI Bpu1102I BsaBI
BsaJI BsaWI BseAI BsiEI BsiWI
BsmI Bsp1407I BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstXI BstZ17I CelII
CfoI Cfr10I CpoI DraI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI HhaI HinP1I
HpaII KasI KpnI KspI MamI
MluI MluNI MroI MscI MslI
MspI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI StyI
SwaI Tth111I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 98 GACGTC GACGT^C
AciI 1518 GCGG CCGC(-3/-1)
AcsI 1768 AAATTC [GA]^AATT[CT]
AcyI 98 GACGTC G[GA]^CG[CT]C
AflII 786 CTTAAG C^TTAAG
AflIII 40 ACACGT A^C[GA][CT]GT
Alw44I 1537 GTGCAC G^TGCAC
AlwNI 947 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1035 CCTCAGG CC^T[ACGT]AGG
ApaLI 1537 GTGCAC G^TGCAC
ApoI 1768 AAATTC [GA]^AATT[CT]
AvaI 1074 CTCGGG C^[CT]CG[GA]G
BamHI 1461 GGATCC G^GATCC
BanI 1091 GGTGCC G^G[CT][GA]CC
BbvCI 270 GCTGAGG CCTCAGC(-5/-2)
BfrI 786 CTTAAG C^TTAAG
BglII 1850 AGATCT A^GATCT
BsaAI 181 CACGTA [CT]AC^GT[GA]
BsaHI 98 GACGTC G[GA]^CG[CT]C
BsmBI 683 CGTCTC CGTCTC(1/5)
BsmFI 1067 GTCCC GGGAC(10/14)
BsoBI 1074 CTCGGG C^[CT]CG[GA]G
BspDI 741 ATCGAT AT^CGAT
Bsu36I 1035 CCTCAGG CC^T[ACGT]AGG
ClaI 741 ATCGAT AT^CGAT
DraIII 1162 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1058 GACTTCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1035 CCTCAGG CC^T[ACGT]AGG
EcoRV 175 GATATC GAT^ATC
Hin1I 98 GACGTC G[GA]^CG[CT]C
HincII 1017 GTTAAC GT[CT]^[GA]AC
HindII 1017 GTTAAC GT[CT]^[GA]AC
HpaI 1017 GTTAAC GTT^AAC
MfeI 156 CAATTG C^AATTG
MunI 156 CAATTG C^AATTG
NheI 612 GCTAGC G^CTAGC
PflMI 1161 CCACAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1065 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 1351 CTGCAG CTGCA^G
SapI 1637 GCTCTTC GCTCTTC(1/4)
SphI 379 GCATGC GCATG^C
Tsp45I 535 GTGAC ^GT[GC]AC
Van91I 1161 CCACAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1070 CCAGCTCGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AfaI 248 GTAC GT^AC
1175 GTAC
AvaII 495 GGACC G^G[AT]CC
1066 GGTCC
BsaI 309 GAGACC GGTCTC(1/5)
1406 GAGACC
BsgI 151 CTGCAC GTGCAG(16/14)
549 CTGCAC
BstBI 116 TTCGAA TT^CGAA
1358 TTCGAA
HindIII 163 AAGCTT A^AGCTT
556 AAGCTT
MspA1I 196 CAGCTG C[AC]G^C[GT]G
950 CAGCTG
NspI 379 GCATGC [GA]CATG^[CT]
875 ACATGC
NspV 116 TTCGAA TT^CGAA
1358 TTCGAA
PvuII 196 CAGCTG CAG^CTG
950 CAGCTG
RsaI 248 GTAC GT^AC
1175 GTAC
SfuI 116 TTCGAA TT^CGAA
1358 TTCGAA
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AluI AlwI AspHI BanII BbsI
BbvI BfaI BmyI BpmI BpuAI
BseRI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV PleI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XhoII