################################## # NO CUT ENZYMES ( 111 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI AhdI Alw44I ApaI ApaLI AscI Asp700I Asp718I AspEI AspI AviII BamHI BanI BbeI BbrPI BcnI BfrI BglI BglII BlpI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstXI BstZ17I CelII Cfr10I CpoI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoRV FseI FspI Hin1I KasI KpnI KspI MamI MfeI MroI MunI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I Van91I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 2098 GTCTAC GT^[AC][GT]AC AccII 1552 CGCG CG^CG AlwNI 905 CAGTAACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 616 CCTTAGG CC^T[ACGT]AGG Aor51HI 2057 AGCGCT AGC^GCT AseI 339 ATTAAT AT^TAAT AsnI 339 ATTAAT AT^TAAT AvaI 937 CCCGAG C^[CT]CG[GA]G AvaII 762 GGTCC G^G[AT]CC BbvCI 945 CCTCAGC CCTCAGC(-5/-2) BcgI 634 CGAGACTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 870 GGATAC GTATCC(6/5) BclI 1989 TGATCA T^GATCA BsaAI 2044 CACGTA [CT]AC^GT[GA] BsmI 464 GAATGC GAATGC(1/-1) BsoBI 937 CCCGAG C^[CT]CG[GA]G Bsp1407I 1119 TGTACA T^GTACA BspDI 1277 ATCGAT AT^CGAT BspLU11I 1811 ACATGT A^CATGT BsrBI 824 CCGCTC CCGCTC(-3/-3) BsrGI 1119 TGTACA T^GTACA BstUI 1552 CGCG CG^CG Bsu36I 616 CCTTAGG CC^T[ACGT]AGG ClaI 1277 ATCGAT AT^CGAT DraII 761 AGGTCCT [GA]G^G[ACGT]CC[CT] Eco47III 2057 AGCGCT AGC^GCT Eco81I 616 CCTTAGG CC^T[ACGT]AGG EcoNI 765 CCTTGTGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 761 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoRI 20 GAATTC G^AATTC FauI 1580 GCGGG CCCGC(4/6) FbaI 1989 TGATCA T^GATCA HapII 30 CCGG C^CGG HgaI 2280 GCGTC GACGC(5/10) HindIII 2154 AAGCTT A^AGCTT HpaII 30 CCGG C^CGG MaeII 2045 ACGT A^CGT MluI 1551 ACGCGT A^CGCGT MslI 1942 CACGCTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 781 CAGCTG C[AC]G^C[GT]G MspI 30 CCGG C^CGG MvnI 1552 CGCG CG^CG NdeI 330 CATATG CA^TATG PpuMI 761 AGGTCCT [GA]G^G[AT]CC[CT] PshBI 339 ATTAAT AT^TAAT PvuII 781 CAGCTG CAG^CTG SacI 208 GAGCTC GAGCT^C ScaI 1382 AGTACT AGT^ACT SspBI 1119 TGTACA T^GTACA XbaI 81 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 38 enzymes) # ################################### AcsI 20 GAATTC [GA]^AATT[CT] 2347 AAATTT ApoI 20 GAATTC [GA]^AATT[CT] 2347 AAATTT AspHI 208 GAGCTC G[AT]GC[AT]^C 940 GAGCAC AvrII 1239 CCTAGG C^CTAGG 1636 CCTAGG BalI 326 TGGCCA TGG^CCA 1299 TGGCCA BanII 208 GAGCTC G[GA]GC[CT]^C 933 GAGCCC BlnI 1239 CCTAGG C^CTAGG 1636 CCTAGG BsiHKAI 208 GAGCTC G[AT]GC[AT]^C 940 GAGCAC BsiYI 765 CCTTGTGTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1302 CCAGTCTGCGG BslI 765 CCTTGTGTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1302 CCAGTCTGCGG BsmFI 304 GGGAC GGGAC(10/14) 2274 GTCCC BsrDI 12 CATTGC GCAATG(2/0) 1695 CATTGC BstEII 775 GGTAACC G^GT[ACGT]ACC 2308 GGTAACC BstPI 775 GGTAACC G^GT[ACGT]ACC 2308 GGTAACC BstYI 201 GGATCT [GA]^GATC[CT] 2189 AGATCC CfoI 221 GCGC GCG^C 2058 GCGC Cfr13I 762 GGTCC G^G[ACGT]CC 2087 GGGCC DsaI 113 CCGTGG C^C[GA][CT]GG 1451 CCGTGG EarI 273 CTCTTC CTCTTC(1/4) 161 GAAGAG EcoO65I 775 GGTAACC G^GT[ACGT]ACC 2308 GGTAACC EcoT22I 1251 ATGCAT ATGCA^T 2354 ATGCAT HaeII 220 AGCGCC [GA]GCGC^[CT] 2057 AGCGCT HhaI 221 GCGC GCG^C 2058 GCGC HinP1I 221 GCGC G^CGC 2058 GCGC HpaI 1732 GTTAAC GTT^AAC 2000 GTTAAC Ksp632I 273 CTCTTC CTCTTC(1/4) 161 GAAGAG MflI 201 GGATCT [GA]^GATC[CT] 2189 AGATCC MluNI 326 TGGCCA TGG^CCA 1299 TGGCCA MscI 326 TGGCCA TGG^CCA 1299 TGGCCA NlaIV 447 GGAACC GG[ACGT]^[ACGT]CC 2173 GGAACC NsiI 1251 ATGCAT ATGCA^T 2354 ATGCAT Sau96I 762 GGTCC G^G[ACGT]CC 2087 GGGCC SfcI 1386 CTGTAG C^T[GA][CT]AG 1863 CTGTAG SmlI 524 CTCAAG C^T[CT][GA]AG 892 CTCAAG TaqI 1133 TCGA T^CGA 1278 TCGA TthHB8I 1133 TCGA T^CGA 1278 TCGA XcmI 218 CCAGCGCCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 270 CCACTCTTCTAGTGG XhoII 201 GGATCT [GA]^GATC[CT] 2189 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AciI AfaI AflIII AluI AlwI BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I DdeI DpnII DraI EaeI EcoRII EcoT14I Fnu4HI FokI HaeIII HincII HindII HinfI HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NspI PleI RsaI Sau3AI ScrFI SfaNI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI