##################################
# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspI AviII BamHI BanI BbeI
BbrPI BcnI BfrI BglI BglII
BlpI Bpu1102I BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BspEI BspHI BspMI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstXI
BstZ17I CelII Cfr10I CpoI DraIII
DrdI EagI Eam1105I EclXI Eco52I
EcoRV FseI FspI Hin1I KasI
KpnI KspI MamI MfeI MroI
MunI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI SwaI
Tth111I Van91I XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccI 2098 GTCTAC GT^[AC][GT]AC
AccII 1552 CGCG CG^CG
AlwNI 905 CAGTAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 616 CCTTAGG CC^T[ACGT]AGG
Aor51HI 2057 AGCGCT AGC^GCT
AseI 339 ATTAAT AT^TAAT
AsnI 339 ATTAAT AT^TAAT
AvaI 937 CCCGAG C^[CT]CG[GA]G
AvaII 762 GGTCC G^G[AT]CC
BbvCI 945 CCTCAGC CCTCAGC(-5/-2)
BcgI 634 CGAGACTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 870 GGATAC GTATCC(6/5)
BclI 1989 TGATCA T^GATCA
BsaAI 2044 CACGTA [CT]AC^GT[GA]
BsmI 464 GAATGC GAATGC(1/-1)
BsoBI 937 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1119 TGTACA T^GTACA
BspDI 1277 ATCGAT AT^CGAT
BspLU11I 1811 ACATGT A^CATGT
BsrBI 824 CCGCTC CCGCTC(-3/-3)
BsrGI 1119 TGTACA T^GTACA
BstUI 1552 CGCG CG^CG
Bsu36I 616 CCTTAGG CC^T[ACGT]AGG
ClaI 1277 ATCGAT AT^CGAT
DraII 761 AGGTCCT [GA]G^G[ACGT]CC[CT]
Eco47III 2057 AGCGCT AGC^GCT
Eco81I 616 CCTTAGG CC^T[ACGT]AGG
EcoNI 765 CCTTGTGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 761 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 20 GAATTC G^AATTC
FauI 1580 GCGGG CCCGC(4/6)
FbaI 1989 TGATCA T^GATCA
HapII 30 CCGG C^CGG
HgaI 2280 GCGTC GACGC(5/10)
HindIII 2154 AAGCTT A^AGCTT
HpaII 30 CCGG C^CGG
MaeII 2045 ACGT A^CGT
MluI 1551 ACGCGT A^CGCGT
MslI 1942 CACGCTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 781 CAGCTG C[AC]G^C[GT]G
MspI 30 CCGG C^CGG
MvnI 1552 CGCG CG^CG
NdeI 330 CATATG CA^TATG
PpuMI 761 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 339 ATTAAT AT^TAAT
PvuII 781 CAGCTG CAG^CTG
SacI 208 GAGCTC GAGCT^C
ScaI 1382 AGTACT AGT^ACT
SspBI 1119 TGTACA T^GTACA
XbaI 81 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AcsI 20 GAATTC [GA]^AATT[CT]
2347 AAATTT
ApoI 20 GAATTC [GA]^AATT[CT]
2347 AAATTT
AspHI 208 GAGCTC G[AT]GC[AT]^C
940 GAGCAC
AvrII 1239 CCTAGG C^CTAGG
1636 CCTAGG
BalI 326 TGGCCA TGG^CCA
1299 TGGCCA
BanII 208 GAGCTC G[GA]GC[CT]^C
933 GAGCCC
BlnI 1239 CCTAGG C^CTAGG
1636 CCTAGG
BsiHKAI 208 GAGCTC G[AT]GC[AT]^C
940 GAGCAC
BsiYI 765 CCTTGTGTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1302 CCAGTCTGCGG
BslI 765 CCTTGTGTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1302 CCAGTCTGCGG
BsmFI 304 GGGAC GGGAC(10/14)
2274 GTCCC
BsrDI 12 CATTGC GCAATG(2/0)
1695 CATTGC
BstEII 775 GGTAACC G^GT[ACGT]ACC
2308 GGTAACC
BstPI 775 GGTAACC G^GT[ACGT]ACC
2308 GGTAACC
BstYI 201 GGATCT [GA]^GATC[CT]
2189 AGATCC
CfoI 221 GCGC GCG^C
2058 GCGC
Cfr13I 762 GGTCC G^G[ACGT]CC
2087 GGGCC
DsaI 113 CCGTGG C^C[GA][CT]GG
1451 CCGTGG
EarI 273 CTCTTC CTCTTC(1/4)
161 GAAGAG
EcoO65I 775 GGTAACC G^GT[ACGT]ACC
2308 GGTAACC
EcoT22I 1251 ATGCAT ATGCA^T
2354 ATGCAT
HaeII 220 AGCGCC [GA]GCGC^[CT]
2057 AGCGCT
HhaI 221 GCGC GCG^C
2058 GCGC
HinP1I 221 GCGC G^CGC
2058 GCGC
HpaI 1732 GTTAAC GTT^AAC
2000 GTTAAC
Ksp632I 273 CTCTTC CTCTTC(1/4)
161 GAAGAG
MflI 201 GGATCT [GA]^GATC[CT]
2189 AGATCC
MluNI 326 TGGCCA TGG^CCA
1299 TGGCCA
MscI 326 TGGCCA TGG^CCA
1299 TGGCCA
NlaIV 447 GGAACC GG[ACGT]^[ACGT]CC
2173 GGAACC
NsiI 1251 ATGCAT ATGCA^T
2354 ATGCAT
Sau96I 762 GGTCC G^G[ACGT]CC
2087 GGGCC
SfcI 1386 CTGTAG C^T[GA][CT]AG
1863 CTGTAG
SmlI 524 CTCAAG C^T[CT][GA]AG
892 CTCAAG
TaqI 1133 TCGA T^CGA
1278 TCGA
TthHB8I 1133 TCGA T^CGA
1278 TCGA
XcmI 218 CCAGCGCCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
270 CCACTCTTCTAGTGG
XhoII 201 GGATCT [GA]^GATC[CT]
2189 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I DdeI DpnII DraI EaeI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HincII HindII HinfI HphI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NspI PleI RsaI Sau3AI ScrFI
SfaNI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI