##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspI AviII BamHI BbvCI
BcgI BciVI BfrI BglII BsaBI
BsaWI BseAI BsgI BsiWI BspEI
BspLU11I BsrBI Bst1107I BstAPI BstXI
BstZ17I Bsu36I DraI DraIII Eam1105I
Eco47III Eco81I EcoRV EcoT22I FseI
FspI HincII HindII HpaI KpnI
MamI MfeI MluI MroI MunI
NheI NruI NsiI PacI PaeR7I
PflFI PflMI PinAI PmeI PshAI
Psp1406I PvuI SalI ScaI SexAI
SfiI SgrAI SmaI SnaBI SpeI
SphI SspI SwaI Tth111I Van91I
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AlwNI 1006 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 408 ATTAAT AT^TAAT
AsnI 408 ATTAAT AT^TAAT
AvaI 1390 CCCGAG C^[CT]CG[GA]G
AvrII 2142 CCTAGG C^CTAGG
BbeI 227 GGCGCC GGCGC^C
BbrPI 2282 CACGTG CAC^GTG
BclI 871 TGATCA T^GATCA
BlnI 2142 CCTAGG C^CTAGG
BlpI 1992 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1992 GCTGAGC GC^T[ACGT]AGC
BsaAI 2282 CACGTG [CT]AC^GT[GA]
BseRI 2300 CTCCTC GAGGAG(10/8)
BsoBI 1390 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1097 TGTACA T^GTACA
BspHI 1868 TCATGA T^CATGA
BspMI 2318 ACCTGC ACCTGC(4/8)
BsrDI 1731 GCAATG GCAATG(2/0)
BsrGI 1097 TGTACA T^GTACA
BssHII 248 GCGCGC G^CGCGC
BssSI 2323 CTCGTG CACGAG(-5/-1)
BstBI 2017 TTCGAA TT^CGAA
BstEII 104 GGTTACC G^GT[ACGT]ACC
BstPI 104 GGTTACC G^GT[ACGT]ACC
BstYI 1523 GGATCT [GA]^GATC[CT]
CelII 1992 GCTGAGC GC^T[ACGT]AGC
CpoI 151 CGGTCCG CG^G[AT]CCG
DraII 473 AGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 738 GACAGTACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2137 CCTTTCCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 473 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 104 GGTTACC G^GT[ACGT]ACC
EcoRI 2118 GAATTC G^AATTC
FbaI 871 TGATCA T^GATCA
HaeII 227 GGCGCC [GA]GCGC^[CT]
HgaI 291 GCGTC GACGC(5/10)
HindIII 2007 AAGCTT A^AGCTT
KasI 227 GGCGCC G^GCGCC
MflI 1523 GGATCT [GA]^GATC[CT]
NarI 227 GGCGCC GG^CGCC
NdeI 1140 CATATG CA^TATG
NspI 333 GCATGT [GA]CATG^[CT]
NspV 2017 TTCGAA TT^CGAA
PmaCI 2282 CACGTG CAC^GTG
PmlI 2282 CACGTG CAC^GTG
PpuMI 473 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 408 ATTAAT AT^TAAT
PstI 840 CTGCAG CTGCA^G
RcaI 1868 TCATGA T^CATGA
RsrII 151 CGGTCCG CG^G[AT]CCG
SacI 272 GAGCTC GAGCT^C
SapI 1119 GAAGAGC GCTCTTC(1/4)
SbfI 839 CCTGCAGG CCTGCA^GG
SfcI 840 CTGCAG C^T[GA][CT]AG
SfoI 227 GGCGCC GGC^GCC
SfuI 2017 TTCGAA TT^CGAA
Sse8387I 839 CCTGCAGG CCTGCA^GG
SspBI 1097 TGTACA T^GTACA
XbaI 668 TCTAGA T^CTAGA
XhoII 1523 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 227 GGCGCC G[GA]^CG[CT]C
290 GGCGTC
AflIII 1447 ACGTGT A^C[GA][CT]GT
2283 ACGTGT
Asp700I 1084 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1887 GAACAGTTTC
BalI 1002 TGGCCA TGG^CCA
2056 TGGCCA
BanI 227 GGCGCC G^G[CT][GA]CC
834 GGTGCC
BsaHI 227 GGCGCC G[GA]^CG[CT]C
290 GGCGTC
BsaI 32 GAGACC GGTCTC(1/5)
462 GAGACC
BsmBI 232 CGTCTC CGTCTC(1/5)
727 GAGACG
BsmFI 391 GGGAC GGGAC(10/14)
47 GTCCC
BsmI 580 GAATGC GAATGC(1/-1)
436 GCATTC
BspDI 354 ATCGAT AT^CGAT
1066 ATCGAT
ClaI 354 ATCGAT AT^CGAT
1066 ATCGAT
Hin1I 227 GGCGCC G[GA]^CG[CT]C
290 GGCGTC
KspI 71 CCGCGG CCGC^GG
267 CCGCGG
MluNI 1002 TGGCCA TGG^CCA
2056 TGGCCA
MscI 1002 TGGCCA TGG^CCA
2056 TGGCCA
MslI 828 CATGCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1422 CACCATGATG
NaeI 157 GCCGGC GCC^GGC
169 GCCGGC
NcoI 286 CCATGG C^CATGG
2109 CCATGG
NgoMIV 157 GCCGGC G^CCGGC
169 GCCGGC
NotI 203 GCGGCCGC GC^GGCCGC
279 GCGGCCGC
PvuII 54 CAGCTG CAG^CTG
638 CAGCTG
SacII 71 CCGCGG CCGC^GG
267 CCGCGG
SmlI 375 CTTGAG C^T[CT][GA]AG
2203 CTCAAG
XmnI 1084 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1887 GAACAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaII BanII
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaJI BsiEI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DsaI EaeI EagI
EarI EclXI Eco52I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I