################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII AccIII AcsI AcyI AflII AgeI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BclI BfrI BglII BlnI BsaAI BsaHI BsaI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspHI BspLU11I BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I ClaI CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HindIII KasI KspI MfeI MflI MluNI MroI MscI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I StuI SwaI Tth111I XcmI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### Acc65I 578 GGTACC G^GTACC AccII 680 CGCG CG^CG AclI 721 AACGTT AA^CGTT AlwI 458 GATCC GGATC(4/5) AocI 399 CCTCAGG CC^T[ACGT]AGG Asp718I 578 GGTACC G^GTACC BciVI 1218 GTATCC GTATCC(6/5) BcnI 27 CCGGG CC^[GC]GG BglI 1047 GCCACAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 336 GCTGAGC GC^T[ACGT]AGC Bpu1102I 336 GCTGAGC GC^T[ACGT]AGC BsaBI 1692 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1403 ACCGGA [AT]^CCGG[AT] BseRI 94 GAGGAG GAGGAG(10/8) BsmAI 498 GTCTC GTCTC(1/5) BsmFI 752 GTCCC GGGAC(10/14) Bsp1407I 1659 TGTACA T^GTACA BsrBI 200 GAGCGG CCGCTC(-3/-3) BsrGI 1659 TGTACA T^GTACA BstUI 680 CGCG CG^CG Bsu36I 399 CCTCAGG CC^T[ACGT]AGG CelII 336 GCTGAGC GC^T[ACGT]AGC EarI 523 GAAGAG CTCTTC(1/4) Eco81I 399 CCTCAGG CC^T[ACGT]AGG HaeII 1036 AGCGCC [GA]GCGC^[CT] HgaI 678 GACGC GACGC(5/10) HpaI 1551 GTTAAC GTT^AAC KpnI 578 GGTACC GGTAC^C Ksp632I 523 GAAGAG CTCTTC(1/4) MamI 1692 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 679 ACGCGT A^CGCGT MslI 1683 CACACAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 680 CGCG CG^CG NaeI 37 GCCGGC GCC^GGC NciI 27 CCGGG CC^[GC]GG NgoMIV 37 GCCGGC G^CCGGC NspI 1521 GCATGC [GA]CATG^[CT] PaeR7I 791 CTCGAG C^TCGAG PflMI 1003 CCACCTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1254 AGGTCCT [GA]G^G[AT]CC[CT] Psp1406I 721 AACGTT AA^CGTT PvuII 334 CAGCTG CAG^CTG SacI 316 GAGCTC GAGCT^C SfiI 1046 GGCCACAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SphI 1521 GCATGC GCATG^C SspBI 1659 TGTACA T^GTACA SspI 1713 AATATT AAT^ATT Van91I 1003 CCACCTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 791 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AflIII 641 ACGTGT A^C[GA][CT]GT 679 ACGCGT AspHI 316 GAGCTC G[AT]GC[AT]^C 1366 GAGCAC AvaI 56 CCCGAG C^[CT]CG[GA]G 791 CTCGAG BanI 578 GGTACC G^G[CT][GA]CC 1400 GGCACC BfaI 1154 CTAG C^TAG 1307 CTAG BsiHKAI 316 GAGCTC G[AT]GC[AT]^C 1366 GAGCAC BsmI 273 GAATGC GAATGC(1/-1) 1393 GCATTC BsoBI 56 CCCGAG C^[CT]CG[GA]G 791 CTCGAG BspMI 1341 ACCTGC ACCTGC(4/8) 1252 GCAGGT BsrDI 588 CATTGC GCAATG(2/0) 1317 GCAATG BsrFI 5 GCCGGT [GA]^CCGG[CT] 37 GCCGGC Cfr10I 5 GCCGGT [GA]^CCGG[CT] 37 GCCGGC HincII 675 GTTGAC GT[CT]^[GA]AC 1551 GTTAAC HindII 675 GTTGAC GT[CT]^[GA]AC 1551 GTTAAC MaeI 1154 CTAG C^TAG 1307 CTAG MspA1I 334 CAGCTG C[AC]G^C[GT]G 1051 CAGCGG PleI 1075 GACTC GAGTC(4/5) 511 GAGTC PstI 358 CTGCAG CTGCA^G 1250 CTGCAG XbaI 1153 TCTAGA T^CTAGA 1306 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 75 enzymes) # ######################################### AccI AciI AfaI AhdI AluI AlwNI AspEI AvaII BanII BbsI BbvI BmyI BpmI BpuAI BsaJI BsgI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI Eam1105I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NcoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I