##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII AccIII AcsI AcyI AflII
AgeI Alw44I Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
AspI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BclI
BfrI BglII BlnI BsaAI BsaHI
BsaI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BspHI BspLU11I BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I ClaI
CpoI DraI DraIII DrdI EagI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI Hin1I HindIII KasI KspI
MfeI MflI MluNI MroI MscI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I StuI SwaI Tth111I
XcmI XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 578 GGTACC G^GTACC
AccII 680 CGCG CG^CG
AclI 721 AACGTT AA^CGTT
AlwI 458 GATCC GGATC(4/5)
AocI 399 CCTCAGG CC^T[ACGT]AGG
Asp718I 578 GGTACC G^GTACC
BciVI 1218 GTATCC GTATCC(6/5)
BcnI 27 CCGGG CC^[GC]GG
BglI 1047 GCCACAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 336 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 336 GCTGAGC GC^T[ACGT]AGC
BsaBI 1692 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1403 ACCGGA [AT]^CCGG[AT]
BseRI 94 GAGGAG GAGGAG(10/8)
BsmAI 498 GTCTC GTCTC(1/5)
BsmFI 752 GTCCC GGGAC(10/14)
Bsp1407I 1659 TGTACA T^GTACA
BsrBI 200 GAGCGG CCGCTC(-3/-3)
BsrGI 1659 TGTACA T^GTACA
BstUI 680 CGCG CG^CG
Bsu36I 399 CCTCAGG CC^T[ACGT]AGG
CelII 336 GCTGAGC GC^T[ACGT]AGC
EarI 523 GAAGAG CTCTTC(1/4)
Eco81I 399 CCTCAGG CC^T[ACGT]AGG
HaeII 1036 AGCGCC [GA]GCGC^[CT]
HgaI 678 GACGC GACGC(5/10)
HpaI 1551 GTTAAC GTT^AAC
KpnI 578 GGTACC GGTAC^C
Ksp632I 523 GAAGAG CTCTTC(1/4)
MamI 1692 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 679 ACGCGT A^CGCGT
MslI 1683 CACACAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 680 CGCG CG^CG
NaeI 37 GCCGGC GCC^GGC
NciI 27 CCGGG CC^[GC]GG
NgoMIV 37 GCCGGC G^CCGGC
NspI 1521 GCATGC [GA]CATG^[CT]
PaeR7I 791 CTCGAG C^TCGAG
PflMI 1003 CCACCTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1254 AGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 721 AACGTT AA^CGTT
PvuII 334 CAGCTG CAG^CTG
SacI 316 GAGCTC GAGCT^C
SfiI 1046 GGCCACAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1521 GCATGC GCATG^C
SspBI 1659 TGTACA T^GTACA
SspI 1713 AATATT AAT^ATT
Van91I 1003 CCACCTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 791 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflIII 641 ACGTGT A^C[GA][CT]GT
679 ACGCGT
AspHI 316 GAGCTC G[AT]GC[AT]^C
1366 GAGCAC
AvaI 56 CCCGAG C^[CT]CG[GA]G
791 CTCGAG
BanI 578 GGTACC G^G[CT][GA]CC
1400 GGCACC
BfaI 1154 CTAG C^TAG
1307 CTAG
BsiHKAI 316 GAGCTC G[AT]GC[AT]^C
1366 GAGCAC
BsmI 273 GAATGC GAATGC(1/-1)
1393 GCATTC
BsoBI 56 CCCGAG C^[CT]CG[GA]G
791 CTCGAG
BspMI 1341 ACCTGC ACCTGC(4/8)
1252 GCAGGT
BsrDI 588 CATTGC GCAATG(2/0)
1317 GCAATG
BsrFI 5 GCCGGT [GA]^CCGG[CT]
37 GCCGGC
Cfr10I 5 GCCGGT [GA]^CCGG[CT]
37 GCCGGC
HincII 675 GTTGAC GT[CT]^[GA]AC
1551 GTTAAC
HindII 675 GTTGAC GT[CT]^[GA]AC
1551 GTTAAC
MaeI 1154 CTAG C^TAG
1307 CTAG
MspA1I 334 CAGCTG C[AC]G^C[GT]G
1051 CAGCGG
PleI 1075 GACTC GAGTC(4/5)
511 GAGTC
PstI 358 CTGCAG CTGCA^G
1250 CTGCAG
XbaI 1153 TCTAGA T^CTAGA
1306 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccI AciI AfaI AhdI AluI
AlwNI AspEI AvaII BanII BbsI
BbvI BmyI BpmI BpuAI BsaJI
BsgI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI Eam1105I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MseI MspI MvaI MwoI
NcoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I