##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
ApaI AscI AseI AsnI Asp718I
AspI AviII BamHI BbeI BcgI
BclI BfrI BglII BsaBI BsiEI
BsiWI BsmI Bsp1407I BspHI BspLU11I
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I CpoI
DraI DrdI EagI EclXI Eco52I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MunI NarI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 676 GTAGAC GT^[AC][GT]AC
AccII 1729 CGCG CG^CG
AccIII 1274 TCCGGA T^CCGGA
AcsI 2409 AAATTT [GA]^AATT[CT]
AflIII 2012 ACACGT A^C[GA][CT]GT
AhdI 421 GACACCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2304 GTGCAC G^TGCAC
AlwI 1371 GATCC GGATC(4/5)
AlwNI 794 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 580 CCTCAGG CC^T[ACGT]AGG
Aor51HI 147 AGCGCT AGC^GCT
ApaLI 2304 GTGCAC G^TGCAC
ApoI 2409 AAATTT [GA]^AATT[CT]
AspEI 421 GACACCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 184 GGCACC G^G[CT][GA]CC
BbrPI 1756 CACGTG CAC^GTG
BbvCI 634 GCTGAGG CCTCAGC(-5/-2)
BciVI 2624 GTATCC GTATCC(6/5)
BglI 1801 GCCCAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 990 GAGACC GGTCTC(1/5)
BsaWI 1274 TCCGGA [AT]^CCGG[AT]
BseAI 1274 TCCGGA T^CCGGA
BsmBI 1570 GAGACG CGTCTC(1/5)
BspEI 1274 TCCGGA T^CCGGA
BsrBI 454 GAGCGG CCGCTC(-3/-3)
BsrDI 304 CATTGC GCAATG(2/0)
BsrFI 2453 GCCGGC [GA]^CCGG[CT]
BstUI 1729 CGCG CG^CG
BstYI 1370 AGATCC [GA]^GATC[CT]
Bsu36I 580 CCTCAGG CC^T[ACGT]AGG
Cfr10I 2453 GCCGGC [GA]^CCGG[CT]
DraIII 683 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 421 GACACCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 147 AGCGCT AGC^GCT
Eco81I 580 CCTCAGG CC^T[ACGT]AGG
MflI 1370 AGATCC [GA]^GATC[CT]
MroI 1274 TCCGGA T^CCGGA
MslI 2297 CATATAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 1729 CGCG CG^CG
NaeI 2453 GCCGGC GCC^GGC
NcoI 509 CCATGG C^CATGG
NdeI 1626 CATATG CA^TATG
NgoMIV 2453 GCCGGC G^CCGGC
PmaCI 1756 CACGTG CAC^GTG
PmlI 1756 CACGTG CAC^GTG
PstI 171 CTGCAG CTGCA^G
SapI 297 GCTCTTC GCTCTTC(1/4)
ScaI 831 AGTACT AGT^ACT
XhoII 1370 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 56 GACGCC G[GA]^CG[CT]C
1798 GACGCC
AvrII 1045 CCTAGG C^CTAGG
1660 CCTAGG
BalI 836 TGGCCA TGG^CCA
1889 TGGCCA
BcnI 254 CCGGG CC^[GC]GG
1748 CCCGG
BlnI 1045 CCTAGG C^CTAGG
1660 CCTAGG
BlpI 150 GCTAAGC GC^T[ACGT]AGC
1654 GCTGAGC
Bpu1102I 150 GCTAAGC GC^T[ACGT]AGC
1654 GCTGAGC
BsaAI 1756 CACGTG [CT]AC^GT[GA]
2353 CACGTA
BsaHI 56 GACGCC G[GA]^CG[CT]C
1798 GACGCC
BsgI 91 GTGCAG GTGCAG(16/14)
202 GTGCAG
BsmFI 858 GGGAC GGGAC(10/14)
1746 GTCCC
BspDI 1648 ATCGAT AT^CGAT
2070 ATCGAT
BspMI 1167 ACCTGC ACCTGC(4/8)
1384 GCAGGT
CelII 150 GCTAAGC GC^T[ACGT]AGC
1654 GCTGAGC
ClaI 1648 ATCGAT AT^CGAT
2070 ATCGAT
EcoNI 712 CCTGAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1425 CCTTTGGAAGG
Hin1I 56 GACGCC G[GA]^CG[CT]C
1798 GACGCC
MluNI 836 TGGCCA TGG^CCA
1889 TGGCCA
MscI 836 TGGCCA TGG^CCA
1889 TGGCCA
MspA1I 1447 CAGCGG C[AC]G^C[GT]G
1784 CCGCTG
NciI 254 CCGGG CC^[GC]GG
1748 CCCGG
SfcI 171 CTGCAG C^T[GA][CT]AG
2549 CTACAG
SmlI 1399 CTCAAG C^T[CT][GA]AG
1942 CTCAAG
TaqI 1649 TCGA T^CGA
2071 TCGA
TfiI 2068 GAATC G^A[AT]TC
2404 GAATC
TthHB8I 1649 TCGA T^CGA
2071 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AfaI AluI Asp700I AspHI
AvaI AvaII BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI StuI StyI Tru9I
TseI Tsp45I Tsp509I TspRI XmnI