##################################
# NO CUT ENZYMES (  103 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
ApaI        AscI        AseI        AsnI        Asp718I     
AspI        AviII       BamHI       BbeI        BcgI        
BclI        BfrI        BglII       BsaBI       BsiEI       
BsiWI       BsmI        Bsp1407I    BspHI       BspLU11I    
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstZ17I     CpoI        
DraI        DrdI        EagI        EclXI       Eco52I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MunI        NarI        
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmeI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacII       SalI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Tth111I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccI          676  GTAGAC               GT^[AC][GT]AC
AccII        1729  CGCG                 CG^CG
AccIII       1274  TCCGGA               T^CCGGA
AcsI         2409  AAATTT               [GA]^AATT[CT]
AflIII       2012  ACACGT               A^C[GA][CT]GT
AhdI          421  GACACCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       2304  GTGCAC               G^TGCAC
AlwI         1371  GATCC                GGATC(4/5)
AlwNI         794  CAGGGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          580  CCTCAGG              CC^T[ACGT]AGG
Aor51HI       147  AGCGCT               AGC^GCT
ApaLI        2304  GTGCAC               G^TGCAC
ApoI         2409  AAATTT               [GA]^AATT[CT]
AspEI         421  GACACCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI          184  GGCACC               G^G[CT][GA]CC
BbrPI        1756  CACGTG               CAC^GTG
BbvCI         634  GCTGAGG              CCTCAGC(-5/-2)
BciVI        2624  GTATCC               GTATCC(6/5)
BglI         1801  GCCCAGAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI          990  GAGACC               GGTCTC(1/5)
BsaWI        1274  TCCGGA               [AT]^CCGG[AT]
BseAI        1274  TCCGGA               T^CCGGA
BsmBI        1570  GAGACG               CGTCTC(1/5)
BspEI        1274  TCCGGA               T^CCGGA
BsrBI         454  GAGCGG               CCGCTC(-3/-3)
BsrDI         304  CATTGC               GCAATG(2/0)
BsrFI        2453  GCCGGC               [GA]^CCGG[CT]
BstUI        1729  CGCG                 CG^CG
BstYI        1370  AGATCC               [GA]^GATC[CT]
Bsu36I        580  CCTCAGG              CC^T[ACGT]AGG
Cfr10I       2453  GCCGGC               [GA]^CCGG[CT]
DraIII        683  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I      421  GACACCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      147  AGCGCT               AGC^GCT
Eco81I        580  CCTCAGG              CC^T[ACGT]AGG
MflI         1370  AGATCC               [GA]^GATC[CT]
MroI         1274  TCCGGA               T^CCGGA
MslI         2297  CATATAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI         1729  CGCG                 CG^CG
NaeI         2453  GCCGGC               GCC^GGC
NcoI          509  CCATGG               C^CATGG
NdeI         1626  CATATG               CA^TATG
NgoMIV       2453  GCCGGC               G^CCGGC
PmaCI        1756  CACGTG               CAC^GTG
PmlI         1756  CACGTG               CAC^GTG
PstI          171  CTGCAG               CTGCA^G
SapI          297  GCTCTTC              GCTCTTC(1/4)
ScaI          831  AGTACT               AGT^ACT
XhoII        1370  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AcyI           56  GACGCC               G[GA]^CG[CT]C
             1798  GACGCC              
AvrII        1045  CCTAGG               C^CTAGG
             1660  CCTAGG              
BalI          836  TGGCCA               TGG^CCA
             1889  TGGCCA              
BcnI          254  CCGGG                CC^[GC]GG
             1748  CCCGG               
BlnI         1045  CCTAGG               C^CTAGG
             1660  CCTAGG              
BlpI          150  GCTAAGC              GC^T[ACGT]AGC
             1654  GCTGAGC             
Bpu1102I      150  GCTAAGC              GC^T[ACGT]AGC
             1654  GCTGAGC             
BsaAI        1756  CACGTG               [CT]AC^GT[GA]
             2353  CACGTA              
BsaHI          56  GACGCC               G[GA]^CG[CT]C
             1798  GACGCC              
BsgI           91  GTGCAG               GTGCAG(16/14)
              202  GTGCAG              
BsmFI         858  GGGAC                GGGAC(10/14)
             1746  GTCCC               
BspDI        1648  ATCGAT               AT^CGAT
             2070  ATCGAT              
BspMI        1167  ACCTGC               ACCTGC(4/8)
             1384  GCAGGT              
CelII         150  GCTAAGC              GC^T[ACGT]AGC
             1654  GCTGAGC             
ClaI         1648  ATCGAT               AT^CGAT
             2070  ATCGAT              
EcoNI         712  CCTGAGCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1425  CCTTTGGAAGG         
Hin1I          56  GACGCC               G[GA]^CG[CT]C
             1798  GACGCC              
MluNI         836  TGGCCA               TGG^CCA
             1889  TGGCCA              
MscI          836  TGGCCA               TGG^CCA
             1889  TGGCCA              
MspA1I       1447  CAGCGG               C[AC]G^C[GT]G
             1784  CCGCTG              
NciI          254  CCGGG                CC^[GC]GG
             1748  CCCGG               
SfcI          171  CTGCAG               C^T[GA][CT]AG
             2549  CTACAG              
SmlI         1399  CTCAAG               C^T[CT][GA]AG
             1942  CTCAAG              
TaqI         1649  TCGA                 T^CGA
             2071  TCGA                
TfiI         2068  GAATC                G^A[AT]TC
             2404  GAATC               
TthHB8I      1649  TCGA                 T^CGA
             2071  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   80 enzymes) #
#########################################

AciI        AfaI        AluI        Asp700I     AspHI       
AvaI        AvaII       BanII       BbsI        BbvI        
BfaI        BmyI        BpmI        BpuAI       BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsoBI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstXI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MnlI        MseI        MspI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PleI        RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       StuI        StyI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       XmnI