################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## AatII Acc65I AclI AflII AgeI ApaI AscI AseI AsnI Asp718I AspI AviII BamHI BbeI BcgI BclI BfrI BglII BsaBI BsiEI BsiWI BsmI Bsp1407I BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CpoI DraI DrdI EagI EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MunI NarI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccI 676 GTAGAC GT^[AC][GT]AC AccII 1729 CGCG CG^CG AccIII 1274 TCCGGA T^CCGGA AcsI 2409 AAATTT [GA]^AATT[CT] AflIII 2012 ACACGT A^C[GA][CT]GT AhdI 421 GACACCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2304 GTGCAC G^TGCAC AlwI 1371 GATCC GGATC(4/5) AlwNI 794 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 580 CCTCAGG CC^T[ACGT]AGG Aor51HI 147 AGCGCT AGC^GCT ApaLI 2304 GTGCAC G^TGCAC ApoI 2409 AAATTT [GA]^AATT[CT] AspEI 421 GACACCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BanI 184 GGCACC G^G[CT][GA]CC BbrPI 1756 CACGTG CAC^GTG BbvCI 634 GCTGAGG CCTCAGC(-5/-2) BciVI 2624 GTATCC GTATCC(6/5) BglI 1801 GCCCAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaI 990 GAGACC GGTCTC(1/5) BsaWI 1274 TCCGGA [AT]^CCGG[AT] BseAI 1274 TCCGGA T^CCGGA BsmBI 1570 GAGACG CGTCTC(1/5) BspEI 1274 TCCGGA T^CCGGA BsrBI 454 GAGCGG CCGCTC(-3/-3) BsrDI 304 CATTGC GCAATG(2/0) BsrFI 2453 GCCGGC [GA]^CCGG[CT] BstUI 1729 CGCG CG^CG BstYI 1370 AGATCC [GA]^GATC[CT] Bsu36I 580 CCTCAGG CC^T[ACGT]AGG Cfr10I 2453 GCCGGC [GA]^CCGG[CT] DraIII 683 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 421 GACACCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 147 AGCGCT AGC^GCT Eco81I 580 CCTCAGG CC^T[ACGT]AGG MflI 1370 AGATCC [GA]^GATC[CT] MroI 1274 TCCGGA T^CCGGA MslI 2297 CATATAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 1729 CGCG CG^CG NaeI 2453 GCCGGC GCC^GGC NcoI 509 CCATGG C^CATGG NdeI 1626 CATATG CA^TATG NgoMIV 2453 GCCGGC G^CCGGC PmaCI 1756 CACGTG CAC^GTG PmlI 1756 CACGTG CAC^GTG PstI 171 CTGCAG CTGCA^G SapI 297 GCTCTTC GCTCTTC(1/4) ScaI 831 AGTACT AGT^ACT XhoII 1370 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcyI 56 GACGCC G[GA]^CG[CT]C 1798 GACGCC AvrII 1045 CCTAGG C^CTAGG 1660 CCTAGG BalI 836 TGGCCA TGG^CCA 1889 TGGCCA BcnI 254 CCGGG CC^[GC]GG 1748 CCCGG BlnI 1045 CCTAGG C^CTAGG 1660 CCTAGG BlpI 150 GCTAAGC GC^T[ACGT]AGC 1654 GCTGAGC Bpu1102I 150 GCTAAGC GC^T[ACGT]AGC 1654 GCTGAGC BsaAI 1756 CACGTG [CT]AC^GT[GA] 2353 CACGTA BsaHI 56 GACGCC G[GA]^CG[CT]C 1798 GACGCC BsgI 91 GTGCAG GTGCAG(16/14) 202 GTGCAG BsmFI 858 GGGAC GGGAC(10/14) 1746 GTCCC BspDI 1648 ATCGAT AT^CGAT 2070 ATCGAT BspMI 1167 ACCTGC ACCTGC(4/8) 1384 GCAGGT CelII 150 GCTAAGC GC^T[ACGT]AGC 1654 GCTGAGC ClaI 1648 ATCGAT AT^CGAT 2070 ATCGAT EcoNI 712 CCTGAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1425 CCTTTGGAAGG Hin1I 56 GACGCC G[GA]^CG[CT]C 1798 GACGCC MluNI 836 TGGCCA TGG^CCA 1889 TGGCCA MscI 836 TGGCCA TGG^CCA 1889 TGGCCA MspA1I 1447 CAGCGG C[AC]G^C[GT]G 1784 CCGCTG NciI 254 CCGGG CC^[GC]GG 1748 CCCGG SfcI 171 CTGCAG C^T[GA][CT]AG 2549 CTACAG SmlI 1399 CTCAAG C^T[CT][GA]AG 1942 CTCAAG TaqI 1649 TCGA T^CGA 2071 TCGA TfiI 2068 GAATC G^A[AT]TC 2404 GAATC TthHB8I 1649 TCGA T^CGA 2071 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 80 enzymes) # ######################################### AciI AfaI AluI Asp700I AspHI AvaI AvaII BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI SacI Sau3AI Sau96I ScrFI SfaNI StuI StyI Tru9I TseI Tsp45I Tsp509I TspRI XmnI