##################################
# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstUI Bsu36I CelII Cfr10I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I Van91I
XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AflIII 469 ACACGT A^C[GA][CT]GT
AlwNI 407 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 513 AGCGCT AGC^GCT
ApaI 1534 GGGCCC GGGCC^C
Asp700I 697 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 1534 GGGCCC G[GA]GC[CT]^C
BclI 1557 TGATCA T^GATCA
BcnI 165 CCCGG CC^[GC]GG
BseRI 734 GAGGAG GAGGAG(10/8)
BsmBI 1693 GAGACG CGTCTC(1/5)
BspMI 376 ACCTGC ACCTGC(4/8)
BsrBI 506 GAGCGG CCGCTC(-3/-3)
BsrDI 94 CATTGC GCAATG(2/0)
Bst1107I 1229 GTATAC GTA^TAC
BstZ17I 1229 GTATAC GTA^TAC
DraI 1390 TTTAAA TTT^AAA
DraII 1534 GGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 923 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 513 AGCGCT AGC^GCT
EcoO109I 1534 GGGCCCC [GA]G^G[ACGT]CC[CT]
FbaI 1557 TGATCA T^GATCA
HgaI 1695 GACGC GACGC(5/10)
MaeII 471 ACGT A^CGT
MslI 1575 CATCACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 943 CAGCGG C[AC]G^C[GT]G
NciI 165 CCCGG CC^[GC]GG
NspI 1598 ACATGC [GA]CATG^[CT]
PaeR7I 1038 CTCGAG C^TCGAG
PshAI 185 GACTCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 378 CTGCAG CTGCA^G
SapI 1163 GAAGAGC GCTCTTC(1/4)
ScaI 1194 AGTACT AGT^ACT
StuI 986 AGGCCT AGG^CCT
XbaI 691 TCTAGA T^CTAGA
XhoI 1038 CTCGAG C^TCGAG
XmnI 697 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 473 GTCTAC GT^[AC][GT]AC
1229 GTATAC
AcsI 817 AAATTT [GA]^AATT[CT]
857 AAATTC
ApoI 817 AAATTT [GA]^AATT[CT]
857 AAATTC
BbsI 872 GAAGAC GAAGAC(2/6)
976 GTCTTC
BpmI 355 CTCCAG CTGGAG(16/14)
626 CTGGAG
BpuAI 872 GAAGAC GAAGAC(2/6)
976 GTCTTC
BstXI 763 CCAGCTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
813 CCAAAAATTTGG
BstYI 1145 GGATCT [GA]^GATC[CT]
1636 GGATCT
FauI 156 CCCGC CCCGC(4/6)
1479 CCCGC
HaeII 492 GGCGCT [GA]GCGC^[CT]
513 AGCGCT
HapII 166 CCGG C^CGG
706 CCGG
HpaII 166 CCGG C^CGG
706 CCGG
MaeIII 409 GTCAC ^GT[ACGT]AC
1292 GTCAC
MflI 1145 GGATCT [GA]^GATC[CT]
1636 GGATCT
MseI 501 TTAA T^TAA
1391 TTAA
MspI 166 CCGG C^CGG
706 CCGG
NcoI 894 CCATGG C^CATGG
1530 CCATGG
TfiI 317 GATTC G^A[AT]TC
1730 GATTC
Tru9I 501 TTAA T^TAA
1391 TTAA
Tsp45I 409 GTCAC ^GT[GC]AC
1292 GTCAC
XhoII 1145 GGATCT [GA]^GATC[CT]
1636 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI AvaII BalI BanI BbvI
BfaI BmyI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HhaI HinP1I HinfI
HphI ItaI Ksp632I MaeI MboI
MboII MluNI MnlI MscI MvaI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TseI
Tsp509I TspRI TthHB8I