################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI Aor51HI ApaI AscI Asp718I AspEI AspI AvaI AviII AvrII BamHI BanII BbeI BcgI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BseAI BsgI BsiEI BsiWI BsoBI Bsp1407I BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I CelII CfoI Cfr10I CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoT22I FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MamI MluI MroI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PshAI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 602 GTCTAC GT^[AC][GT]AC AflIII 2083 ACGTGT A^C[GA][CT]GT Alw44I 2235 GTGCAC G^TGCAC ApaLI 2235 GTGCAC G^TGCAC AspHI 2235 GTGCAC G[AT]GC[AT]^C BalI 817 TGGCCA TGG^CCA BbrPI 2082 CACGTG CAC^GTG BbvCI 1382 CCTCAGC CCTCAGC(-5/-2) BciVI 1568 GGATAC GTATCC(6/5) BsaAI 2082 CACGTG [CT]AC^GT[GA] BsaWI 1003 TCCGGT [AT]^CCGG[AT] BsiHKAI 2235 GTGCAC G[AT]GC[AT]^C BsmBI 607 CGTCTC CGTCTC(1/5) BspDI 1437 ATCGAT AT^CGAT BstXI 1204 CCAATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ClaI 1437 ATCGAT AT^CGAT DraIII 201 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 346 CCATGG C^C[GA][CT]GG EarI 182 GAAGAG CTCTTC(1/4) EcoRV 1485 GATATC GAT^ATC EcoT14I 346 CCATGG C^C[AT][AT]GG FauI 1630 GCGGG CCCGC(4/6) HapII 1004 CCGG C^CGG HincII 172 GTTGAC GT[CT]^[GA]AC HindII 172 GTTGAC GT[CT]^[GA]AC HpaII 1004 CCGG C^CGG Ksp632I 182 GAAGAG CTCTTC(1/4) MfeI 855 CAATTG C^AATTG MluNI 817 TGGCCA TGG^CCA MscI 817 TGGCCA TGG^CCA MspI 1004 CCGG C^CGG MunI 855 CAATTG C^AATTG NcoI 346 CCATGG C^CATGG PflMI 258 CCAAACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 1000 GAGTC GAGTC(4/5) PmaCI 2082 CACGTG CAC^GTG PmeI 529 GTTTAAAC GTTT^AAAC PmlI 2082 CACGTG CAC^GTG PpuMI 1346 AGGACCT [GA]G^G[AT]CC[CT] SapI 182 GAAGAGC GCTCTTC(1/4) SspI 1962 AATATT AAT^ATT StyI 346 CCATGG C^C[AT][AT]GG Van91I 258 CCAAACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcsI 866 AAATTT [GA]^AATT[CT] 2254 GAATTT AfaI 1018 GTAC GT^AC 1829 GTAC ApoI 866 AAATTT [GA]^AATT[CT] 2254 GAATTT AseI 1780 ATTAAT AT^TAAT 2034 ATTAAT AsnI 1780 ATTAAT AT^TAAT 2034 ATTAAT Asp700I 22 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1510 GAAGCAATTC AvaII 1347 GGACC G^G[AT]CC 2211 GGTCC BclI 919 TGATCA T^GATCA 1352 TGATCA BpmI 372 CTCCAG CTGGAG(16/14) 590 CTCCAG BsmI 622 GAATGC GAATGC(1/-1) 2129 GAATGC BspHI 916 TCATGA T^CATGA 2115 TCATGA BspMI 667 ACCTGC ACCTGC(4/8) 1022 ACCTGC BstAPI 377 GCAGCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2133 GCAGGGCTTGC BstYI 834 GGATCT [GA]^GATC[CT] 1501 AGATCC DraI 530 TTTAAA TTT^AAA 1671 TTTAAA DraII 224 AGGCCCC [GA]G^G[ACGT]CC[CT] 1346 AGGACCT EcoO109I 224 AGGCCCC [GA]G^G[ACGT]CC[CT] 1346 AGGACCT FbaI 919 TGATCA T^GATCA 1352 TGATCA MflI 834 GGATCT [GA]^GATC[CT] 1501 AGATCC PshBI 1780 ATTAAT AT^TAAT 2034 ATTAAT RcaI 916 TCATGA T^CATGA 2115 TCATGA RsaI 1018 GTAC GT^AC 1829 GTAC TaqI 1438 TCGA T^CGA 2252 TCGA Tsp45I 699 GTCAC ^GT[GC]AC 963 GTGAC TthHB8I 1438 TCGA T^CGA 2252 TCGA XhoII 834 GGATCT [GA]^GATC[CT] 1501 AGATCC XmnI 22 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1510 GAAGCAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AciI AluI AlwI AlwNI AocI BanI BbsI BbvI BfaI BmyI BpuAI BsaI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Bsu36I Cac8I Cfr13I DdeI DpnII EaeI Eco81I EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI PstI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TfiI Tru9I TseI Tsp509I TspRI