##################################
# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Aor51HI
ApaI AscI Asp718I AspEI AspI
AvaI AviII AvrII BamHI BanII
BbeI BcgI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaBI
BsaHI BseAI BsgI BsiEI BsiWI
BsoBI Bsp1407I BspEI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I CelII CfoI Cfr10I CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoT22I
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HindIII HpaI KasI
KpnI KspI MamI MluI MroI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 602 GTCTAC GT^[AC][GT]AC
AflIII 2083 ACGTGT A^C[GA][CT]GT
Alw44I 2235 GTGCAC G^TGCAC
ApaLI 2235 GTGCAC G^TGCAC
AspHI 2235 GTGCAC G[AT]GC[AT]^C
BalI 817 TGGCCA TGG^CCA
BbrPI 2082 CACGTG CAC^GTG
BbvCI 1382 CCTCAGC CCTCAGC(-5/-2)
BciVI 1568 GGATAC GTATCC(6/5)
BsaAI 2082 CACGTG [CT]AC^GT[GA]
BsaWI 1003 TCCGGT [AT]^CCGG[AT]
BsiHKAI 2235 GTGCAC G[AT]GC[AT]^C
BsmBI 607 CGTCTC CGTCTC(1/5)
BspDI 1437 ATCGAT AT^CGAT
BstXI 1204 CCAATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 1437 ATCGAT AT^CGAT
DraIII 201 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 346 CCATGG C^C[GA][CT]GG
EarI 182 GAAGAG CTCTTC(1/4)
EcoRV 1485 GATATC GAT^ATC
EcoT14I 346 CCATGG C^C[AT][AT]GG
FauI 1630 GCGGG CCCGC(4/6)
HapII 1004 CCGG C^CGG
HincII 172 GTTGAC GT[CT]^[GA]AC
HindII 172 GTTGAC GT[CT]^[GA]AC
HpaII 1004 CCGG C^CGG
Ksp632I 182 GAAGAG CTCTTC(1/4)
MfeI 855 CAATTG C^AATTG
MluNI 817 TGGCCA TGG^CCA
MscI 817 TGGCCA TGG^CCA
MspI 1004 CCGG C^CGG
MunI 855 CAATTG C^AATTG
NcoI 346 CCATGG C^CATGG
PflMI 258 CCAAACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1000 GAGTC GAGTC(4/5)
PmaCI 2082 CACGTG CAC^GTG
PmeI 529 GTTTAAAC GTTT^AAAC
PmlI 2082 CACGTG CAC^GTG
PpuMI 1346 AGGACCT [GA]G^G[AT]CC[CT]
SapI 182 GAAGAGC GCTCTTC(1/4)
SspI 1962 AATATT AAT^ATT
StyI 346 CCATGG C^C[AT][AT]GG
Van91I 258 CCAAACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 866 AAATTT [GA]^AATT[CT]
2254 GAATTT
AfaI 1018 GTAC GT^AC
1829 GTAC
ApoI 866 AAATTT [GA]^AATT[CT]
2254 GAATTT
AseI 1780 ATTAAT AT^TAAT
2034 ATTAAT
AsnI 1780 ATTAAT AT^TAAT
2034 ATTAAT
Asp700I 22 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1510 GAAGCAATTC
AvaII 1347 GGACC G^G[AT]CC
2211 GGTCC
BclI 919 TGATCA T^GATCA
1352 TGATCA
BpmI 372 CTCCAG CTGGAG(16/14)
590 CTCCAG
BsmI 622 GAATGC GAATGC(1/-1)
2129 GAATGC
BspHI 916 TCATGA T^CATGA
2115 TCATGA
BspMI 667 ACCTGC ACCTGC(4/8)
1022 ACCTGC
BstAPI 377 GCAGCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2133 GCAGGGCTTGC
BstYI 834 GGATCT [GA]^GATC[CT]
1501 AGATCC
DraI 530 TTTAAA TTT^AAA
1671 TTTAAA
DraII 224 AGGCCCC [GA]G^G[ACGT]CC[CT]
1346 AGGACCT
EcoO109I 224 AGGCCCC [GA]G^G[ACGT]CC[CT]
1346 AGGACCT
FbaI 919 TGATCA T^GATCA
1352 TGATCA
MflI 834 GGATCT [GA]^GATC[CT]
1501 AGATCC
PshBI 1780 ATTAAT AT^TAAT
2034 ATTAAT
RcaI 916 TCATGA T^CATGA
2115 TCATGA
RsaI 1018 GTAC GT^AC
1829 GTAC
TaqI 1438 TCGA T^CGA
2252 TCGA
Tsp45I 699 GTCAC ^GT[GC]AC
963 GTGAC
TthHB8I 1438 TCGA T^CGA
2252 TCGA
XhoII 834 GGATCT [GA]^GATC[CT]
1501 AGATCC
XmnI 22 GAAGTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1510 GAAGCAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AluI AlwI AlwNI AocI
BanI BbsI BbvI BfaI BmyI
BpuAI BsaI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Bsu36I Cac8I
Cfr13I DdeI DpnII EaeI Eco81I
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV NspI PstI PvuII Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TfiI Tru9I TseI Tsp509I TspRI