##################################
# NO CUT ENZYMES (  123 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Aor51HI     
ApaI        AscI        Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BamHI       BanII       
BbeI        BcgI        BcnI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BsaBI       
BsaHI       BseAI       BsgI        BsiEI       BsiWI       
BsoBI       Bsp1407I    BspEI       BspLU11I    BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstZ17I     CelII       CfoI        Cfr10I      CpoI        
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRI       EcoT22I     
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HindIII     HpaI        KasI        
KpnI        KspI        MamI        MluI        MroI        
MvnI        NaeI        NarI        NciI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PshAI       Psp1406I    PvuI        RsrII       SacI        
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        SwaI        Tth111I     XbaI        XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccI          602  GTCTAC               GT^[AC][GT]AC
AflIII       2083  ACGTGT               A^C[GA][CT]GT
Alw44I       2235  GTGCAC               G^TGCAC
ApaLI        2235  GTGCAC               G^TGCAC
AspHI        2235  GTGCAC               G[AT]GC[AT]^C
BalI          817  TGGCCA               TGG^CCA
BbrPI        2082  CACGTG               CAC^GTG
BbvCI        1382  CCTCAGC              CCTCAGC(-5/-2)
BciVI        1568  GGATAC               GTATCC(6/5)
BsaAI        2082  CACGTG               [CT]AC^GT[GA]
BsaWI        1003  TCCGGT               [AT]^CCGG[AT]
BsiHKAI      2235  GTGCAC               G[AT]GC[AT]^C
BsmBI         607  CGTCTC               CGTCTC(1/5)
BspDI        1437  ATCGAT               AT^CGAT
BstXI        1204  CCAATCAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI         1437  ATCGAT               AT^CGAT
DraIII        201  CACACTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          346  CCATGG               C^C[GA][CT]GG
EarI          182  GAAGAG               CTCTTC(1/4)
EcoRV        1485  GATATC               GAT^ATC
EcoT14I       346  CCATGG               C^C[AT][AT]GG
FauI         1630  GCGGG                CCCGC(4/6)
HapII        1004  CCGG                 C^CGG
HincII        172  GTTGAC               GT[CT]^[GA]AC
HindII        172  GTTGAC               GT[CT]^[GA]AC
HpaII        1004  CCGG                 C^CGG
Ksp632I       182  GAAGAG               CTCTTC(1/4)
MfeI          855  CAATTG               C^AATTG
MluNI         817  TGGCCA               TGG^CCA
MscI          817  TGGCCA               TGG^CCA
MspI         1004  CCGG                 C^CGG
MunI          855  CAATTG               C^AATTG
NcoI          346  CCATGG               C^CATGG
PflMI         258  CCAAACACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI         1000  GAGTC                GAGTC(4/5)
PmaCI        2082  CACGTG               CAC^GTG
PmeI          529  GTTTAAAC             GTTT^AAAC
PmlI         2082  CACGTG               CAC^GTG
PpuMI        1346  AGGACCT              [GA]G^G[AT]CC[CT]
SapI          182  GAAGAGC              GCTCTTC(1/4)
SspI         1962  AATATT               AAT^ATT
StyI          346  CCATGG               C^C[AT][AT]GG
Van91I        258  CCAAACACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AcsI          866  AAATTT               [GA]^AATT[CT]
             2254  GAATTT              
AfaI         1018  GTAC                 GT^AC
             1829  GTAC                
ApoI          866  AAATTT               [GA]^AATT[CT]
             2254  GAATTT              
AseI         1780  ATTAAT               AT^TAAT
             2034  ATTAAT              
AsnI         1780  ATTAAT               AT^TAAT
             2034  ATTAAT              
Asp700I        22  GAAGTAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1510  GAAGCAATTC          
AvaII        1347  GGACC                G^G[AT]CC
             2211  GGTCC               
BclI          919  TGATCA               T^GATCA
             1352  TGATCA              
BpmI          372  CTCCAG               CTGGAG(16/14)
              590  CTCCAG              
BsmI          622  GAATGC               GAATGC(1/-1)
             2129  GAATGC              
BspHI         916  TCATGA               T^CATGA
             2115  TCATGA              
BspMI         667  ACCTGC               ACCTGC(4/8)
             1022  ACCTGC              
BstAPI        377  GCAGCAGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2133  GCAGGGCTTGC         
BstYI         834  GGATCT               [GA]^GATC[CT]
             1501  AGATCC              
DraI          530  TTTAAA               TTT^AAA
             1671  TTTAAA              
DraII         224  AGGCCCC              [GA]G^G[ACGT]CC[CT]
             1346  AGGACCT             
EcoO109I      224  AGGCCCC              [GA]G^G[ACGT]CC[CT]
             1346  AGGACCT             
FbaI          919  TGATCA               T^GATCA
             1352  TGATCA              
MflI          834  GGATCT               [GA]^GATC[CT]
             1501  AGATCC              
PshBI        1780  ATTAAT               AT^TAAT
             2034  ATTAAT              
RcaI          916  TCATGA               T^CATGA
             2115  TCATGA              
RsaI         1018  GTAC                 GT^AC
             1829  GTAC                
TaqI         1438  TCGA                 T^CGA
             2252  TCGA                
Tsp45I        699  GTCAC                ^GT[GC]AC
              963  GTGAC               
TthHB8I      1438  TCGA                 T^CGA
             2252  TCGA                
XhoII         834  GGATCT               [GA]^GATC[CT]
             1501  AGATCC              
XmnI           22  GAAGTAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1510  GAAGCAATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AciI        AluI        AlwI        AlwNI       AocI        
BanI        BbsI        BbvI        BfaI        BmyI        
BpuAI       BsaI        BsaJI       BseRI       BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       Bsu36I      Cac8I       
Cfr13I      DdeI        DpnII       EaeI        Eco81I      
EcoRII      Fnu4HI      FokI        HaeIII      HinfI       
HphI        ItaI        MaeI        MaeII       MaeIII      
MboI        MboII       MnlI        MseI        MslI        
MspA1I      MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PstI        PvuII       Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
TfiI        Tru9I       TseI        Tsp509I     TspRI