################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflIII AgeI Alw44I Aor51HI ApaLI AscI Asp718I AviII AvrII BamHI BanI BbeI BbrPI BglII BlnI BsaAI BsaBI BsaHI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstXI BstZ17I Cfr10I ClaI CpoI DraIII EagI EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI MamI MluI MroI NaeI NarI NdeI NgoMIV NheI NotI NsiI NspI NspV PacI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI XbaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AcsI 580 AAATTC [GA]^AATT[CT] AflII 1908 CTTAAG C^TTAAG AhdI 1481 GACGTTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 80 GGGCCC GGGCC^C ApoI 580 AAATTC [GA]^AATT[CT] AseI 865 ATTAAT AT^TAAT AsnI 865 ATTAAT AT^TAAT AspEI 1481 GACGTTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1241 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BbsI 346 GAAGAC GAAGAC(2/6) BbvCI 739 GCTGAGG CCTCAGC(-5/-2) BciVI 1855 GTATCC GTATCC(6/5) BclI 1762 TGATCA T^GATCA BfrI 1908 CTTAAG C^TTAAG BglI 73 GCCCTCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1298 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1298 GCTGAGC GC^T[ACGT]AGC BpuAI 346 GAAGAC GAAGAC(2/6) BsgI 1438 GTGCAG GTGCAG(16/14) BsiEI 68 CGGTCG CG[GA][CT]^CG BsmBI 696 GAGACG CGTCTC(1/5) BsmI 811 GAATGC GAATGC(1/-1) BspHI 1413 TCATGA T^CATGA BspMI 1206 GCAGGT ACCTGC(4/8) BstEII 1997 GGTGACC G^GT[ACGT]ACC BstPI 1997 GGTGACC G^GT[ACGT]ACC CelII 1298 GCTGAGC GC^T[ACGT]AGC DraI 2104 TTTAAA TTT^AAA DrdI 1313 GACATGAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 1481 GACGTTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 1434 CCTGGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1997 GGTGACC G^GT[ACGT]ACC EcoT14I 839 CCATGG C^C[AT][AT]GG FbaI 1762 TGATCA T^GATCA KspI 99 CCGCGG CCGC^GG MaeII 1482 ACGT A^CGT MfeI 263 CAATTG C^AATTG MunI 263 CAATTG C^AATTG NcoI 839 CCATGG C^CATGG PflFI 1241 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 623 GGGACCC [GA]G^G[AT]CC[CT] PshAI 1839 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 865 ATTAAT AT^TAAT PstI 1042 CTGCAG CTGCA^G PvuII 169 CAGCTG CAG^CTG RcaI 1413 TCATGA T^CATGA SacII 99 CCGCGG CCGC^GG SapI 204 GCTCTTC GCTCTTC(1/4) ScaI 872 AGTACT AGT^ACT StyI 839 CCATGG C^C[AT][AT]GG Tth111I 1241 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccI 2069 GTAGAC GT^[AC][GT]AC 2184 GTAGAC AlwI 222 GATCC GGATC(4/5) 783 GATCC Asp700I 1071 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1164 GAACTTTTTC BalI 798 TGGCCA TGG^CCA 1213 TGGCCA BcgI 414 CGACCTGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 385 GCAAGCACCTCG BfaI 253 CTAG C^TAG 640 CTAG BsaI 1142 GAGACC GGTCTC(1/5) 50 GGTCTC BsrBI 85 CCGCTC CCGCTC(-3/-3) 119 GAGCGG BstYI 221 AGATCC [GA]^GATC[CT] 782 AGATCC DsaI 99 CCGCGG C^C[GA][CT]GG 839 CCATGG EaeI 798 TGGCCA [CT]^GGCC[GA] 1213 TGGCCA MaeI 253 CTAG C^TAG 640 CTAG MflI 221 AGATCC [GA]^GATC[CT] 782 AGATCC MluNI 798 TGGCCA TGG^CCA 1213 TGGCCA MscI 798 TGGCCA TGG^CCA 1213 TGGCCA MslI 1728 CATATTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1991 CATATTGGTG MspA1I 99 CCGCGG C[AC]G^C[GT]G 169 CAGCTG NruI 115 TCGCGA TCG^CGA 1034 TCGCGA PaeR7I 901 CTCGAG C^TCGAG 922 CTCGAG PflMI 225 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 300 CCATACAATGG SacI 898 GAGCTC GAGCT^C 1115 GAGCTC SmaI 40 CCCGGG CCC^GGG 242 CCCGGG SspI 1861 AATATT AAT^ATT 2140 AATATT TfiI 941 GATTC G^A[AT]TC 1652 GAATC Van91I 225 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 300 CCATACAATGG XcmI 300 CCATACAATGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 763 CCAGAGAAGCTCTGG XhoI 901 CTCGAG C^TCGAG 922 CTCGAG XhoII 221 AGATCC [GA]^GATC[CT] 782 AGATCC XmaCI 40 CCCGGG C^CCGGG 242 CCCGGG XmaI 40 CCCGGG C^CCGGG 242 CCCGGG XmnI 1071 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1164 GAACTTTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 81 enzymes) # ######################################### AccII AciI AfaI AluI AlwNI AocI AspHI AvaI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstUI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII EarI Eco81I EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MnlI MseI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I