##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflIII AgeI Alw44I Aor51HI ApaLI
AscI Asp718I AviII AvrII BamHI
BanI BbeI BbrPI BglII BlnI
BsaAI BsaBI BsaHI BsaWI BseAI
BsiWI Bsp1407I BspDI BspEI BspLU11I
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstXI BstZ17I Cfr10I
ClaI CpoI DraIII EagI EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
MamI MluI MroI NaeI NarI
NdeI NgoMIV NheI NotI NsiI
NspI NspV PacI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AcsI 580 AAATTC [GA]^AATT[CT]
AflII 1908 CTTAAG C^TTAAG
AhdI 1481 GACGTTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 80 GGGCCC GGGCC^C
ApoI 580 AAATTC [GA]^AATT[CT]
AseI 865 ATTAAT AT^TAAT
AsnI 865 ATTAAT AT^TAAT
AspEI 1481 GACGTTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1241 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 346 GAAGAC GAAGAC(2/6)
BbvCI 739 GCTGAGG CCTCAGC(-5/-2)
BciVI 1855 GTATCC GTATCC(6/5)
BclI 1762 TGATCA T^GATCA
BfrI 1908 CTTAAG C^TTAAG
BglI 73 GCCCTCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1298 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1298 GCTGAGC GC^T[ACGT]AGC
BpuAI 346 GAAGAC GAAGAC(2/6)
BsgI 1438 GTGCAG GTGCAG(16/14)
BsiEI 68 CGGTCG CG[GA][CT]^CG
BsmBI 696 GAGACG CGTCTC(1/5)
BsmI 811 GAATGC GAATGC(1/-1)
BspHI 1413 TCATGA T^CATGA
BspMI 1206 GCAGGT ACCTGC(4/8)
BstEII 1997 GGTGACC G^GT[ACGT]ACC
BstPI 1997 GGTGACC G^GT[ACGT]ACC
CelII 1298 GCTGAGC GC^T[ACGT]AGC
DraI 2104 TTTAAA TTT^AAA
DrdI 1313 GACATGAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1481 GACGTTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1434 CCTGGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1997 GGTGACC G^GT[ACGT]ACC
EcoT14I 839 CCATGG C^C[AT][AT]GG
FbaI 1762 TGATCA T^GATCA
KspI 99 CCGCGG CCGC^GG
MaeII 1482 ACGT A^CGT
MfeI 263 CAATTG C^AATTG
MunI 263 CAATTG C^AATTG
NcoI 839 CCATGG C^CATGG
PflFI 1241 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 623 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 1839 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 865 ATTAAT AT^TAAT
PstI 1042 CTGCAG CTGCA^G
PvuII 169 CAGCTG CAG^CTG
RcaI 1413 TCATGA T^CATGA
SacII 99 CCGCGG CCGC^GG
SapI 204 GCTCTTC GCTCTTC(1/4)
ScaI 872 AGTACT AGT^ACT
StyI 839 CCATGG C^C[AT][AT]GG
Tth111I 1241 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 2069 GTAGAC GT^[AC][GT]AC
2184 GTAGAC
AlwI 222 GATCC GGATC(4/5)
783 GATCC
Asp700I 1071 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1164 GAACTTTTTC
BalI 798 TGGCCA TGG^CCA
1213 TGGCCA
BcgI 414 CGACCTGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
385 GCAAGCACCTCG
BfaI 253 CTAG C^TAG
640 CTAG
BsaI 1142 GAGACC GGTCTC(1/5)
50 GGTCTC
BsrBI 85 CCGCTC CCGCTC(-3/-3)
119 GAGCGG
BstYI 221 AGATCC [GA]^GATC[CT]
782 AGATCC
DsaI 99 CCGCGG C^C[GA][CT]GG
839 CCATGG
EaeI 798 TGGCCA [CT]^GGCC[GA]
1213 TGGCCA
MaeI 253 CTAG C^TAG
640 CTAG
MflI 221 AGATCC [GA]^GATC[CT]
782 AGATCC
MluNI 798 TGGCCA TGG^CCA
1213 TGGCCA
MscI 798 TGGCCA TGG^CCA
1213 TGGCCA
MslI 1728 CATATTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1991 CATATTGGTG
MspA1I 99 CCGCGG C[AC]G^C[GT]G
169 CAGCTG
NruI 115 TCGCGA TCG^CGA
1034 TCGCGA
PaeR7I 901 CTCGAG C^TCGAG
922 CTCGAG
PflMI 225 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
300 CCATACAATGG
SacI 898 GAGCTC GAGCT^C
1115 GAGCTC
SmaI 40 CCCGGG CCC^GGG
242 CCCGGG
SspI 1861 AATATT AAT^ATT
2140 AATATT
TfiI 941 GATTC G^A[AT]TC
1652 GAATC
Van91I 225 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
300 CCATACAATGG
XcmI 300 CCATACAATGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
763 CCAGAGAAGCTCTGG
XhoI 901 CTCGAG C^TCGAG
922 CTCGAG
XhoII 221 AGATCC [GA]^GATC[CT]
782 AGATCC
XmaCI 40 CCCGGG C^CCGGG
242 CCCGGG
XmaI 40 CCCGGG C^CCGGG
242 CCCGGG
XmnI 1071 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1164 GAACTTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AocI AspHI AvaI AvaII BanII
BbvI BcnI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstUI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII EarI Eco81I EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeIII MboI
MboII MnlI MseI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I