##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI Alw44I ApaLI
AscI Asp718I AspEI AspI AvrII
BbrPI BcgI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsiWI BsmBI Bsp1407I
BspDI BspHI BspLU11I BsrDI BsrGI
BssSI Bst1107I BstZ17I CelII ClaI
DrdI Eam1105I EarI EcoNI EcoRI
EcoRV FbaI FseI HgaI HincII
HindII HindIII HpaI KpnI Ksp632I
MamI MfeI MluI MunI NdeI
NheI NotI NruI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
Psp1406I RcaI SalI SapI SbfI
SexAI SfiI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Aor51HI 1801 AGCGCT AGC^GCT
AseI 988 ATTAAT AT^TAAT
AsnI 988 ATTAAT AT^TAAT
Asp700I 978 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 2030 TGCGCA TGC^GCA
BalI 2059 TGGCCA TGG^CCA
BamHI 556 GGATCC G^GATCC
BbsI 1016 GAAGAC GAAGAC(2/6)
BbvCI 1544 CCTCAGC CCTCAGC(-5/-2)
BciVI 813 GGATAC GTATCC(6/5)
BpuAI 1016 GAAGAC GAAGAC(2/6)
BseRI 97 CTCCTC GAGGAG(10/8)
BsmI 904 GCATTC GAATGC(1/-1)
BsrBI 660 GAGCGG CCGCTC(-3/-3)
BssHII 446 GCGCGC G^CGCGC
BstEII 362 GGTGACC G^GT[ACGT]ACC
BstPI 362 GGTGACC G^GT[ACGT]ACC
CpoI 671 CGGTCCG CG^G[AT]CCG
DraI 539 TTTAAA TTT^AAA
DraIII 1359 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1801 AGCGCT AGC^GCT
EcoO65I 362 GGTGACC G^GT[ACGT]ACC
FspI 2030 TGCGCA TGC^GCA
MluNI 2059 TGGCCA TGG^CCA
MscI 2059 TGGCCA TGG^CCA
NaeI 244 GCCGGC GCC^GGC
NgoMIV 244 GCCGGC G^CCGGC
PflMI 1413 CCAAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1735 GACTAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 988 ATTAAT AT^TAAT
PvuI 279 CGATCG CGAT^CG
PvuII 2041 CAGCTG CAG^CTG
RsrII 671 CGGTCCG CG^G[AT]CCG
SacI 211 GAGCTC GAGCT^C
ScaI 1688 AGTACT AGT^ACT
SgrAI 243 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 1716 CTCAAG C^T[CT][GA]AG
Van91I 1413 CCAAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 978 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccIII 396 TCCGGA T^CCGGA
1903 TCCGGA
AcsI 930 AAATTC [GA]^AATT[CT]
1920 AAATTC
AcyI 241 GGCGCC G[GA]^CG[CT]C
719 GGCGCC
AlwNI 1584 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
1791 CAGATGCTG
AocI 1589 CCTGAGG CC^T[ACGT]AGG
1893 CCTAAGG
ApoI 930 AAATTC [GA]^AATT[CT]
1920 AAATTC
BbeI 241 GGCGCC GGCGC^C
719 GGCGCC
BpmI 1250 CTCCAG CTGGAG(16/14)
1811 CTGGAG
BsaHI 241 GGCGCC G[GA]^CG[CT]C
719 GGCGCC
BsaWI 396 TCCGGA [AT]^CCGG[AT]
1903 TCCGGA
BseAI 396 TCCGGA T^CCGGA
1903 TCCGGA
BsgI 1407 CTGCAC GTGCAG(16/14)
1788 GTGCAG
BsmAI 1248 GTCTC GTCTC(1/5)
1694 GTCTC
BspEI 396 TCCGGA T^CCGGA
1903 TCCGGA
BstAPI 1637 GCAAAGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1790 GCAGATGCTGC
BstBI 312 TTCGAA TT^CGAA
1013 TTCGAA
BstXI 1256 CCAAGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1951 CCAGGGACATGG
Bsu36I 1589 CCTGAGG CC^T[ACGT]AGG
1893 CCTAAGG
Eco81I 1589 CCTGAGG CC^T[ACGT]AGG
1893 CCTAAGG
EcoT22I 16 ATGCAT ATGCA^T
1940 ATGCAT
Hin1I 241 GGCGCC G[GA]^CG[CT]C
719 GGCGCC
KasI 241 GGCGCC G^GCGCC
719 GGCGCC
MaeII 317 ACGT A^CGT
1563 ACGT
MroI 396 TCCGGA T^CCGGA
1903 TCCGGA
MslI 1662 CACCTGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1880 CATCACCGTG
NarI 241 GGCGCC GG^CGCC
719 GGCGCC
NsiI 16 ATGCAT ATGCA^T
1940 ATGCAT
NspI 18 GCATGT [GA]CATG^[CT]
139 ACATGC
NspV 312 TTCGAA TT^CGAA
1013 TTCGAA
SfoI 241 GGCGCC GGC^GCC
719 GGCGCC
SfuI 312 TTCGAA TT^CGAA
1013 TTCGAA
SmaI 166 CCCGGG CCC^GGG
469 CCCGGG
TfiI 381 GAATC G^A[AT]TC
393 GATTC
XbaI 127 TCTAGA T^CTAGA
262 TCTAGA
XmaCI 166 CCCGGG C^CCGGG
469 CCCGGG
XmaI 166 CCCGGG C^CCGGG
469 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
ApaI AspHI AvaI AvaII BanI
BanII BbvI BcnI BfaI BmyI
BsaJI BsiEI BsiHKAI BsiYI BslI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EclXI Eco52I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI KspI MaeI
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PleI PpuMI PstI RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII