##################################
# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AgeI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AspHI AvaI AviII AvrII BbeI
BbsI BcgI BcnI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
BspDI BspEI BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FokI FseI FspI HaeII HapII
HhaI Hin1I HinP1I HpaII KasI
KpnI MamI MfeI MluI MroI
MspI MunI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmeI PpuMI Psp1406I PvuI
RsrII SacI SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI SwaI TfiI Van91I XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AflII 1441 CTTAAG C^TTAAG
AhdI 102 GACTGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 770 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 102 GACTGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 157 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 347 GGACC G^G[AT]CC
BalI 614 TGGCCA TGG^CCA
BamHI 1776 GGATCC G^GATCC
BanI 1851 GGTGCC G^G[CT][GA]CC
BanII 782 GGGCTC G[GA]GC[CT]^C
BbrPI 1652 CACGTG CAC^GTG
BbvCI 1024 GCTGAGG CCTCAGC(-5/-2)
BciVI 1369 GTATCC GTATCC(6/5)
BfrI 1441 CTTAAG C^TTAAG
BpmI 1500 CTCCAG CTGGAG(16/14)
BsaAI 1652 CACGTG [CT]AC^GT[GA]
BsaI 1030 GAGACC GGTCTC(1/5)
BseRI 1027 GAGGAG GAGGAG(10/8)
BsmFI 510 GGGAC GGGAC(10/14)
Bsp1407I 888 TGTACA T^GTACA
BsrDI 1159 CATTGC GCAATG(2/0)
BsrGI 888 TGTACA T^GTACA
BstYI 1776 GGATCC [GA]^GATC[CT]
Eam1105I 102 GACTGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 115 GAAGAG CTCTTC(1/4)
EcoT14I 1076 CCATGG C^C[AT][AT]GG
FauI 780 GCGGG CCCGC(4/6)
HgaI 404 GACGC GACGC(5/10)
HincII 1984 GTTAAC GT[CT]^[GA]AC
HindII 1984 GTTAAC GT[CT]^[GA]AC
HpaI 1984 GTTAAC GTT^AAC
Ksp632I 115 GAAGAG CTCTTC(1/4)
KspI 678 CCGCGG CCGC^GG
MaeII 1653 ACGT A^CGT
MflI 1776 GGATCC [GA]^GATC[CT]
MluNI 614 TGGCCA TGG^CCA
MscI 614 TGGCCA TGG^CCA
NcoI 1076 CCATGG C^CATGG
PflFI 157 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1652 CACGTG CAC^GTG
PmlI 1652 CACGTG CAC^GTG
PshAI 1606 GACTCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 678 CCGCGG CCGC^GG
SapI 115 GAAGAGC GCTCTTC(1/4)
SspBI 888 TGTACA T^GTACA
SspI 1409 AATATT AAT^ATT
StyI 1076 CCATGG C^C[AT][AT]GG
Tth111I 157 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 197 CCATCATGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1776 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccII 43 CGCG CG^CG
679 CGCG
AflIII 1911 ACATGT A^C[GA][CT]GT
1971 ACATGT
AseI 1095 ATTAAT AT^TAAT
1406 ATTAAT
AsnI 1095 ATTAAT AT^TAAT
1406 ATTAAT
BclI 479 TGATCA T^GATCA
1648 TGATCA
BsiYI 165 CCTTCGATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
691 CCTGGCCCTGG
BslI 165 CCTTCGATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
691 CCTGGCCCTGG
BspLU11I 1911 ACATGT A^CATGT
1971 ACATGT
BsrI 1183 ACTGG ACTGG(1/-1)
2049 CCAGT
BstUI 43 CGCG CG^CG
679 CGCG
EaeI 92 TGGCCG [CT]^GGCC[GA]
614 TGGCCA
FbaI 479 TGATCA T^GATCA
1648 TGATCA
HindIII 1262 AAGCTT A^AGCTT
1638 AAGCTT
MvnI 43 CGCG CG^CG
679 CGCG
NlaIV 1776 GGATCC GG[ACGT]^[ACGT]CC
1851 GGTGCC
PshBI 1095 ATTAAT AT^TAAT
1406 ATTAAT
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbvI BfaI BmyI BsaJI
BsmAI Bsp1286I BspHI BspMI BssKI
BstNI Cac8I Cfr13I DdeI DpnII
DsaI EcoRII Fnu4HI HaeIII HinfI
HphI ItaI MaeI MaeIII MboI
MboII MnlI MseI MslI MspA1I
MvaI MwoI NdeII NlaIII NspI
PleI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I