################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspHI AvaI AviII AvrII BbeI BbsI BcgI BcnI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI BspDI BspEI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FokI FseI FspI HaeII HapII HhaI Hin1I HinP1I HpaII KasI KpnI MamI MfeI MluI MroI MspI MunI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmeI PpuMI Psp1406I PvuI RsrII SacI SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI TfiI Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AflII 1441 CTTAAG C^TTAAG AhdI 102 GACTGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 770 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG AspEI 102 GACTGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 157 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 347 GGACC G^G[AT]CC BalI 614 TGGCCA TGG^CCA BamHI 1776 GGATCC G^GATCC BanI 1851 GGTGCC G^G[CT][GA]CC BanII 782 GGGCTC G[GA]GC[CT]^C BbrPI 1652 CACGTG CAC^GTG BbvCI 1024 GCTGAGG CCTCAGC(-5/-2) BciVI 1369 GTATCC GTATCC(6/5) BfrI 1441 CTTAAG C^TTAAG BpmI 1500 CTCCAG CTGGAG(16/14) BsaAI 1652 CACGTG [CT]AC^GT[GA] BsaI 1030 GAGACC GGTCTC(1/5) BseRI 1027 GAGGAG GAGGAG(10/8) BsmFI 510 GGGAC GGGAC(10/14) Bsp1407I 888 TGTACA T^GTACA BsrDI 1159 CATTGC GCAATG(2/0) BsrGI 888 TGTACA T^GTACA BstYI 1776 GGATCC [GA]^GATC[CT] Eam1105I 102 GACTGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 115 GAAGAG CTCTTC(1/4) EcoT14I 1076 CCATGG C^C[AT][AT]GG FauI 780 GCGGG CCCGC(4/6) HgaI 404 GACGC GACGC(5/10) HincII 1984 GTTAAC GT[CT]^[GA]AC HindII 1984 GTTAAC GT[CT]^[GA]AC HpaI 1984 GTTAAC GTT^AAC Ksp632I 115 GAAGAG CTCTTC(1/4) KspI 678 CCGCGG CCGC^GG MaeII 1653 ACGT A^CGT MflI 1776 GGATCC [GA]^GATC[CT] MluNI 614 TGGCCA TGG^CCA MscI 614 TGGCCA TGG^CCA NcoI 1076 CCATGG C^CATGG PflFI 157 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1652 CACGTG CAC^GTG PmlI 1652 CACGTG CAC^GTG PshAI 1606 GACTCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 678 CCGCGG CCGC^GG SapI 115 GAAGAGC GCTCTTC(1/4) SspBI 888 TGTACA T^GTACA SspI 1409 AATATT AAT^ATT StyI 1076 CCATGG C^C[AT][AT]GG Tth111I 157 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 197 CCATCATGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1776 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AccII 43 CGCG CG^CG 679 CGCG AflIII 1911 ACATGT A^C[GA][CT]GT 1971 ACATGT AseI 1095 ATTAAT AT^TAAT 1406 ATTAAT AsnI 1095 ATTAAT AT^TAAT 1406 ATTAAT BclI 479 TGATCA T^GATCA 1648 TGATCA BsiYI 165 CCTTCGATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 691 CCTGGCCCTGG BslI 165 CCTTCGATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 691 CCTGGCCCTGG BspLU11I 1911 ACATGT A^CATGT 1971 ACATGT BsrI 1183 ACTGG ACTGG(1/-1) 2049 CCAGT BstUI 43 CGCG CG^CG 679 CGCG EaeI 92 TGGCCG [CT]^GGCC[GA] 614 TGGCCA FbaI 479 TGATCA T^GATCA 1648 TGATCA HindIII 1262 AAGCTT A^AGCTT 1638 AAGCTT MvnI 43 CGCG CG^CG 679 CGCG NlaIV 1776 GGATCC GG[ACGT]^[ACGT]CC 1851 GGTGCC PshBI 1095 ATTAAT AT^TAAT 1406 ATTAAT ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI BbvI BfaI BmyI BsaJI BsmAI Bsp1286I BspHI BspMI BssKI BstNI Cac8I Cfr13I DdeI DpnII DsaI EcoRII Fnu4HI HaeIII HinfI HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NspI PleI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I