################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AatII AccII AccIII AclI AcsI AflII AflIII AhdI Alw44I ApaLI ApoI AscI AseI AsnI Asp700I AspEI AviII BbrPI BbsI BcgI BclI BfrI BglII BpuAI BsaBI BsaI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI BstXI ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco52I EcoO65I EcoRI FbaI FseI FspI HincII HindII HindIII HpaI KspI MamI MfeI MluI MroI MseI MunI MvnI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflMI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SbfI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccI 473 GTATAC GT^[AC][GT]AC AgeI 582 ACCGGT A^CCGGT Aor51HI 1034 AGCGCT AGC^GCT AspI 914 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1666 CCCGAG C^[CT]CG[GA]G AvrII 1413 CCTAGG C^CTAGG BamHI 882 GGATCC G^GATCC BbeI 1055 GGCGCC GGCGC^C BbvCI 1187 GCTGAGG CCTCAGC(-5/-2) BciVI 1881 GTATCC GTATCC(6/5) BlnI 1413 CCTAGG C^CTAGG BlpI 1464 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1464 GCTCAGC GC^T[ACGT]AGC BsoBI 1666 CCCGAG C^[CT]CG[GA]G Bst1107I 473 GTATAC GTA^TAC BstZ17I 473 GTATAC GTA^TAC CelII 1464 GCTCAGC GC^T[ACGT]AGC DrdI 911 GACGACCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1034 AGCGCT AGC^GCT EcoNI 1632 CCTACATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 344 GATATC GAT^ATC EcoT22I 1544 ATGCAT ATGCA^T KasI 1055 GGCGCC G^GCGCC NarI 1055 GGCGCC GG^CGCC NsiI 1544 ATGCAT ATGCA^T PflFI 914 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 582 ACCGGT A^CCGGT SacI 190 GAGCTC GAGCT^C SapI 853 GCTCTTC GCTCTTC(1/4) ScaI 591 AGTACT AGT^ACT SexAI 915 ACCAGGT A^CC[AT]GGT SfcI 720 CTGTAG C^T[GA][CT]AG SfoI 1055 GGCGCC GGC^GCC StuI 361 AGGCCT AGG^CCT Tsp509I 311 AATT ^AATT Tth111I 914 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### Acc65I 262 GGTACC G^GTACC 1823 GGTACC AcyI 371 GGCGTC G[GA]^CG[CT]C 1055 GGCGCC AlwNI 1622 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG 1698 CAGCACCTG AocI 396 CCTCAGG CC^T[ACGT]AGG 453 CCTCAGG ApaI 244 GGGCCC GGGCC^C 1533 GGGCCC Asp718I 262 GGTACC G^GTACC 1823 GGTACC AspHI 190 GAGCTC G[AT]GC[AT]^C 1328 GAGCAC BcnI 131 CCGGG CC^[GC]GG 1262 CCGGG BglI 363 GCCTCAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1409 GCCACCTAGGC BsaAI 773 TACGTG [CT]AC^GT[GA] 1019 CACGTA BsaHI 371 GGCGTC G[GA]^CG[CT]C 1055 GGCGCC BseRI 451 CTCCTC GAGGAG(10/8) 647 GAGGAG BsiHKAI 190 GAGCTC G[AT]GC[AT]^C 1328 GAGCAC BstYI 882 GGATCC [GA]^GATC[CT] 1507 GGATCT Bsu36I 396 CCTCAGG CC^T[ACGT]AGG 453 CCTCAGG CfoI 1035 GCGC GCG^C 1056 GCGC DraII 1416 AGGCCCC [GA]G^G[ACGT]CC[CT] 1690 GGGGCCT EarI 61 CTCTTC CTCTTC(1/4) 854 CTCTTC Eco81I 396 CCTCAGG CC^T[ACGT]AGG 453 CCTCAGG EcoO109I 1416 AGGCCCC [GA]G^G[ACGT]CC[CT] 1690 GGGGCCT HaeII 1034 AGCGCT [GA]GCGC^[CT] 1055 GGCGCC HgaI 372 GCGTC GACGC(5/10) 1220 GCGTC HhaI 1035 GCGC GCG^C 1056 GCGC Hin1I 371 GGCGTC G[GA]^CG[CT]C 1055 GGCGCC HinP1I 1035 GCGC G^CGC 1056 GCGC KpnI 262 GGTACC GGTAC^C 1823 GGTACC Ksp632I 61 CTCTTC CTCTTC(1/4) 854 CTCTTC MaeII 774 ACGT A^CGT 1020 ACGT MflI 882 GGATCC [GA]^GATC[CT] 1507 GGATCT NciI 131 CCGGG CC^[GC]GG 1262 CCGGG NspI 714 ACATGC [GA]CATG^[CT] 1104 ACATGC PvuII 164 CAGCTG CAG^CTG 1459 CAGCTG SmlI 377 CTCAAG C^T[CT][GA]AG 1316 CTCAAG XhoII 882 GGATCC [GA]^GATC[CT] 1507 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 75 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BalI BanI BanII BbvI BfaI BmyI BpmI BsaJI BsaWI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI Cac8I Cfr10I Cfr13I DdeI DpnII DsaI EaeI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NcoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I XcmI