##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII AccII AccIII AclI AcsI
AflII AflIII AhdI Alw44I ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AviII BbrPI BbsI BcgI
BclI BfrI BglII BpuAI BsaBI
BsaI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstPI BstUI
BstXI ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRI FbaI FseI FspI HincII
HindII HindIII HpaI KspI MamI
MfeI MluI MroI MseI MunI
MvnI NaeI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflMI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SbfI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Van91I XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccI 473 GTATAC GT^[AC][GT]AC
AgeI 582 ACCGGT A^CCGGT
Aor51HI 1034 AGCGCT AGC^GCT
AspI 914 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1666 CCCGAG C^[CT]CG[GA]G
AvrII 1413 CCTAGG C^CTAGG
BamHI 882 GGATCC G^GATCC
BbeI 1055 GGCGCC GGCGC^C
BbvCI 1187 GCTGAGG CCTCAGC(-5/-2)
BciVI 1881 GTATCC GTATCC(6/5)
BlnI 1413 CCTAGG C^CTAGG
BlpI 1464 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1464 GCTCAGC GC^T[ACGT]AGC
BsoBI 1666 CCCGAG C^[CT]CG[GA]G
Bst1107I 473 GTATAC GTA^TAC
BstZ17I 473 GTATAC GTA^TAC
CelII 1464 GCTCAGC GC^T[ACGT]AGC
DrdI 911 GACGACCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1034 AGCGCT AGC^GCT
EcoNI 1632 CCTACATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 344 GATATC GAT^ATC
EcoT22I 1544 ATGCAT ATGCA^T
KasI 1055 GGCGCC G^GCGCC
NarI 1055 GGCGCC GG^CGCC
NsiI 1544 ATGCAT ATGCA^T
PflFI 914 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 582 ACCGGT A^CCGGT
SacI 190 GAGCTC GAGCT^C
SapI 853 GCTCTTC GCTCTTC(1/4)
ScaI 591 AGTACT AGT^ACT
SexAI 915 ACCAGGT A^CC[AT]GGT
SfcI 720 CTGTAG C^T[GA][CT]AG
SfoI 1055 GGCGCC GGC^GCC
StuI 361 AGGCCT AGG^CCT
Tsp509I 311 AATT ^AATT
Tth111I 914 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 262 GGTACC G^GTACC
1823 GGTACC
AcyI 371 GGCGTC G[GA]^CG[CT]C
1055 GGCGCC
AlwNI 1622 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1698 CAGCACCTG
AocI 396 CCTCAGG CC^T[ACGT]AGG
453 CCTCAGG
ApaI 244 GGGCCC GGGCC^C
1533 GGGCCC
Asp718I 262 GGTACC G^GTACC
1823 GGTACC
AspHI 190 GAGCTC G[AT]GC[AT]^C
1328 GAGCAC
BcnI 131 CCGGG CC^[GC]GG
1262 CCGGG
BglI 363 GCCTCAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1409 GCCACCTAGGC
BsaAI 773 TACGTG [CT]AC^GT[GA]
1019 CACGTA
BsaHI 371 GGCGTC G[GA]^CG[CT]C
1055 GGCGCC
BseRI 451 CTCCTC GAGGAG(10/8)
647 GAGGAG
BsiHKAI 190 GAGCTC G[AT]GC[AT]^C
1328 GAGCAC
BstYI 882 GGATCC [GA]^GATC[CT]
1507 GGATCT
Bsu36I 396 CCTCAGG CC^T[ACGT]AGG
453 CCTCAGG
CfoI 1035 GCGC GCG^C
1056 GCGC
DraII 1416 AGGCCCC [GA]G^G[ACGT]CC[CT]
1690 GGGGCCT
EarI 61 CTCTTC CTCTTC(1/4)
854 CTCTTC
Eco81I 396 CCTCAGG CC^T[ACGT]AGG
453 CCTCAGG
EcoO109I 1416 AGGCCCC [GA]G^G[ACGT]CC[CT]
1690 GGGGCCT
HaeII 1034 AGCGCT [GA]GCGC^[CT]
1055 GGCGCC
HgaI 372 GCGTC GACGC(5/10)
1220 GCGTC
HhaI 1035 GCGC GCG^C
1056 GCGC
Hin1I 371 GGCGTC G[GA]^CG[CT]C
1055 GGCGCC
HinP1I 1035 GCGC G^CGC
1056 GCGC
KpnI 262 GGTACC GGTAC^C
1823 GGTACC
Ksp632I 61 CTCTTC CTCTTC(1/4)
854 CTCTTC
MaeII 774 ACGT A^CGT
1020 ACGT
MflI 882 GGATCC [GA]^GATC[CT]
1507 GGATCT
NciI 131 CCGGG CC^[GC]GG
1262 CCGGG
NspI 714 ACATGC [GA]CATG^[CT]
1104 ACATGC
PvuII 164 CAGCTG CAG^CTG
1459 CAGCTG
SmlI 377 CTCAAG C^T[CT][GA]AG
1316 CTCAAG
XhoII 882 GGATCC [GA]^GATC[CT]
1507 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BalI BanI BanII BbvI BfaI
BmyI BpmI BsaJI BsaWI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI Cac8I Cfr10I Cfr13I
DdeI DpnII DsaI EaeI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NcoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I XcmI