################################## # NO CUT ENZYMES ( 140 enzymes) # ################################## AatII AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI AlwNI Aor51HI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BbeI BbrPI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I CelII ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KspI MaeII MamI MfeI MluI MroI MseI MunI MvnI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TfiI Tru9I Tth111I TthHB8I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### Acc65I 1240 GGTACC G^GTACC AciI 182 GCGG CCGC(-3/-1) Alw44I 117 GTGCAC G^TGCAC ApaLI 117 GTGCAC G^TGCAC Asp718I 1240 GGTACC G^GTACC BalI 1351 TGGCCA TGG^CCA BamHI 1148 GGATCC G^GATCC BpmI 15 CTGGAG CTGGAG(16/14) BsaI 21 GAGACC GGTCTC(1/5) BsgI 672 GTGCAG GTGCAG(16/14) BspMI 464 GCAGGT ACCTGC(4/8) BsrFI 94 GCCGGC [GA]^CCGG[CT] BssSI 114 CTCGTG CACGAG(-5/-1) BstAPI 35 GCACAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cfr10I 94 GCCGGC [GA]^CCGG[CT] EcoNI 1139 CCTGGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HaeII 103 AGCGCC [GA]GCGC^[CT] HphI 645 GGTGA GGTGA(8/7) KpnI 1240 GGTACC GGTAC^C MluNI 1351 TGGCCA TGG^CCA MscI 1351 TGGCCA TGG^CCA MspA1I 331 CAGCTG C[AC]G^C[GT]G NaeI 94 GCCGGC GCC^GGC NgoMIV 94 GCCGGC G^CCGGC NlaIII 399 CATG CATG^ PflMI 365 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 1266 CTGCAG CTGCA^G PvuII 331 CAGCTG CAG^CTG SapI 1301 GCTCTTC GCTCTTC(1/4) SmaI 252 CCCGGG CCC^GGG Van91I 365 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 252 CCCGGG C^CCGGG XmaI 252 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AfaI 30 GTAC GT^AC 1241 GTAC ApaI 165 GGGCCC GGGCC^C 248 GGGCCC AvaI 252 CCCGGG C^[CT]CG[GA]G 413 CCCGAG BbsI 768 GAAGAC GAAGAC(2/6) 903 GAAGAC BpuAI 768 GAAGAC GAAGAC(2/6) 903 GAAGAC BseRI 18 GAGGAG GAGGAG(10/8) 1191 GAGGAG BsoBI 252 CCCGGG C^[CT]CG[GA]G 413 CCCGAG BsrI 658 CCAGT ACTGG(1/-1) 679 CCAGT EaeI 210 CGGCCA [CT]^GGCC[GA] 1351 TGGCCA EarI 1302 CTCTTC CTCTTC(1/4) 434 GAAGAG EcoT14I 379 CCTTGG C^C[AT][AT]GG 1390 CCAAGG Ksp632I 1302 CTCTTC CTCTTC(1/4) 434 GAAGAG MslI 665 CACTCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1343 CACTGAAGTG RsaI 30 GTAC GT^AC 1241 GTAC SacI 111 GAGCTC GAGCT^C 1287 GAGCTC SfcI 1165 CTACAG C^T[GA][CT]AG 1266 CTGCAG SmlI 525 CTTGAG C^T[CT][GA]AG 667 CTCAAG StyI 379 CCTTGG C^C[AT][AT]GG 1390 CCAAGG Tsp45I 172 GTGAC ^GT[GC]AC 1341 GTCAC Tsp509I 512 AATT ^AATT 1256 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AluI AlwI AocI AspHI AvaII BanI BanII BbvCI BbvI BcnI BfaI BmyI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII Eco81I EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeI MaeIII MboI MboII MflI MnlI MspI MvaI MwoI NciI NdeII NlaIV PleI PpuMI Sau3AI Sau96I ScrFI StuI TseI TspRI XcmI XhoII