##################################
# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AlwNI Aor51HI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BbeI BbrPI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KspI
MaeII MamI MfeI MluI MroI
MseI MunI MvnI NarI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TaqI TfiI Tru9I
Tth111I TthHB8I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 1240 GGTACC G^GTACC
AciI 182 GCGG CCGC(-3/-1)
Alw44I 117 GTGCAC G^TGCAC
ApaLI 117 GTGCAC G^TGCAC
Asp718I 1240 GGTACC G^GTACC
BalI 1351 TGGCCA TGG^CCA
BamHI 1148 GGATCC G^GATCC
BpmI 15 CTGGAG CTGGAG(16/14)
BsaI 21 GAGACC GGTCTC(1/5)
BsgI 672 GTGCAG GTGCAG(16/14)
BspMI 464 GCAGGT ACCTGC(4/8)
BsrFI 94 GCCGGC [GA]^CCGG[CT]
BssSI 114 CTCGTG CACGAG(-5/-1)
BstAPI 35 GCACAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 94 GCCGGC [GA]^CCGG[CT]
EcoNI 1139 CCTGGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 103 AGCGCC [GA]GCGC^[CT]
HphI 645 GGTGA GGTGA(8/7)
KpnI 1240 GGTACC GGTAC^C
MluNI 1351 TGGCCA TGG^CCA
MscI 1351 TGGCCA TGG^CCA
MspA1I 331 CAGCTG C[AC]G^C[GT]G
NaeI 94 GCCGGC GCC^GGC
NgoMIV 94 GCCGGC G^CCGGC
NlaIII 399 CATG CATG^
PflMI 365 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1266 CTGCAG CTGCA^G
PvuII 331 CAGCTG CAG^CTG
SapI 1301 GCTCTTC GCTCTTC(1/4)
SmaI 252 CCCGGG CCC^GGG
Van91I 365 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 252 CCCGGG C^CCGGG
XmaI 252 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 30 GTAC GT^AC
1241 GTAC
ApaI 165 GGGCCC GGGCC^C
248 GGGCCC
AvaI 252 CCCGGG C^[CT]CG[GA]G
413 CCCGAG
BbsI 768 GAAGAC GAAGAC(2/6)
903 GAAGAC
BpuAI 768 GAAGAC GAAGAC(2/6)
903 GAAGAC
BseRI 18 GAGGAG GAGGAG(10/8)
1191 GAGGAG
BsoBI 252 CCCGGG C^[CT]CG[GA]G
413 CCCGAG
BsrI 658 CCAGT ACTGG(1/-1)
679 CCAGT
EaeI 210 CGGCCA [CT]^GGCC[GA]
1351 TGGCCA
EarI 1302 CTCTTC CTCTTC(1/4)
434 GAAGAG
EcoT14I 379 CCTTGG C^C[AT][AT]GG
1390 CCAAGG
Ksp632I 1302 CTCTTC CTCTTC(1/4)
434 GAAGAG
MslI 665 CACTCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1343 CACTGAAGTG
RsaI 30 GTAC GT^AC
1241 GTAC
SacI 111 GAGCTC GAGCT^C
1287 GAGCTC
SfcI 1165 CTACAG C^T[GA][CT]AG
1266 CTGCAG
SmlI 525 CTTGAG C^T[CT][GA]AG
667 CTCAAG
StyI 379 CCTTGG C^C[AT][AT]GG
1390 CCAAGG
Tsp45I 172 GTGAC ^GT[GC]AC
1341 GTCAC
Tsp509I 512 AATT ^AATT
1256 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AluI AlwI AocI AspHI AvaII
BanI BanII BbvCI BbvI BcnI
BfaI BmyI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII Eco81I EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MaeI MaeIII
MboI MboII MflI MnlI MspI
MvaI MwoI NciI NdeII NlaIV
PleI PpuMI Sau3AI Sau96I ScrFI
StuI TseI TspRI XcmI XhoII