##################################
# NO CUT ENZYMES ( 132 enzymes) #
##################################
AccII AccIII AclI AcsI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AviII
BbeI BbrPI BbvCI BcgI BcnI
BfrI BglI BglII BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsgI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspLU11I BsrFI BsrGI BssHII BstAPI
BstEII BstPI BstUI Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
HaeII HhaI HinP1I HindIII HpaI
KasI KspI MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp509I Van91I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 1268 GGTACC G^GTACC
AccI 393 GTATAC GT^[AC][GT]AC
Asp718I 1268 GGTACC G^GTACC
AspI 1110 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 469 CCCGAG C^[CT]CG[GA]G
AvrII 837 CCTAGG C^CTAGG
BalI 341 TGGCCA TGG^CCA
BbsI 667 GTCTTC GAAGAC(2/6)
BciVI 1381 GGATAC GTATCC(6/5)
BclI 209 TGATCA T^GATCA
BlnI 837 CCTAGG C^CTAGG
BpuAI 667 GTCTTC GAAGAC(2/6)
BsaI 592 GAGACC GGTCTC(1/5)
BsmBI 378 CGTCTC CGTCTC(1/5)
BsmI 403 GAATGC GAATGC(1/-1)
BsoBI 469 CCCGAG C^[CT]CG[GA]G
BspHI 155 TCATGA T^CATGA
BspMI 759 GCAGGT ACCTGC(4/8)
BsrBI 1090 GAGCGG CCGCTC(-3/-3)
BsrDI 319 GCAATG GCAATG(2/0)
BssSI 556 CTCGTG CACGAG(-5/-1)
Bst1107I 393 GTATAC GTA^TAC
BstBI 674 TTCGAA TT^CGAA
BstXI 344 CCACAGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 393 GTATAC GTA^TAC
DraII 281 AGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 1475 GACCCTGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 341 TGGCCA [CT]^GGCC[GA]
EcoO109I 281 AGGGCCT [GA]G^G[ACGT]CC[CT]
FbaI 209 TGATCA T^GATCA
HapII 400 CCGG C^CGG
HgaI 377 GCGTC GACGC(5/10)
HincII 609 GTTGAC GT[CT]^[GA]AC
HindII 609 GTTGAC GT[CT]^[GA]AC
HpaII 400 CCGG C^CGG
KpnI 1268 GGTACC GGTAC^C
MluNI 341 TGGCCA TGG^CCA
MscI 341 TGGCCA TGG^CCA
MspI 400 CCGG C^CGG
NspV 674 TTCGAA TT^CGAA
PflFI 1110 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 925 CTGCAG CTGCA^G
RcaI 155 TCATGA T^CATGA
SfuI 674 TTCGAA TT^CGAA
TaqI 675 TCGA T^CGA
TfiI 368 GATTC G^A[AT]TC
Tth111I 1110 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 675 TCGA T^CGA
XcmI 120 CCAGAGCACCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 7 enzymes) #
###################################
AatII 355 GACGTC GACGT^C
1076 GACGTC
BseRI 754 GAGGAG GAGGAG(10/8)
1061 GAGGAG
BstF5I 162 CATCC GGATG(2/0)
789 GGATG
FokI 162 CATCC GGATG(9/13)
789 GGATG
MseI 293 TTAA T^TAA
1528 TTAA
SacI 223 GAGCTC GAGCT^C
631 GAGCTC
Tru9I 293 TTAA T^TAA
1528 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AspHI AvaII BamHI BanI
BanII BbvI BfaI BmyI BpmI
BsaHI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DsaI EarI EcoRII EcoT14I
Fnu4HI HaeIII Hin1I HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MvaI MwoI NcoI NdeII
NlaIII NlaIV PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TseI Tsp45I TspRI XhoII