##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
AgeI AhdI Alw44I AlwNI AocI
ApaLI AscI Asp718I AspEI AvaI
AviII AvrII BalI BanI BbeI
BbrPI BbvCI BciVI BclI BfrI
BglI BlnI BsaBI BsaHI BseAI
BsiEI BsiWI BsmBI BsmI BsoBI
BspDI BspEI BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I Cfr10I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO65I EcoT22I
FbaI FseI FspI Hin1I HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MspA1I
MunI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PvuI PvuII RsrII SacII
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SspI StuI SwaI
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 692 GTCGAC GT^[AC][GT]AC
AclI 1076 AACGTT AA^CGTT
Aor51HI 1014 AGCGCT AGC^GCT
ApaI 577 GGGCCC GGGCC^C
AseI 1862 ATTAAT AT^TAAT
AsnI 1862 ATTAAT AT^TAAT
Asp700I 590 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 2540 GGATCC G^GATCC
BcnI 1111 CCGGG CC^[GC]GG
BglII 1505 AGATCT A^GATCT
BlpI 2194 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2194 GCTCAGC GC^T[ACGT]AGC
BsaAI 1178 TACGTG [CT]AC^GT[GA]
BsaI 1156 GGTCTC GGTCTC(1/5)
BsaWI 2109 ACCGGA [AT]^CCGG[AT]
BseRI 188 GAGGAG GAGGAG(10/8)
Bsp1407I 1818 TGTACA T^GTACA
BspHI 793 TCATGA T^CATGA
BspLU11I 1000 ACATGT A^CATGT
BsrGI 1818 TGTACA T^GTACA
BstXI 2042 CCATTTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2194 GCTCAGC GC^T[ACGT]AGC
DraIII 1148 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 766 TGGCCG [CT]^GGCC[GA]
Eco47III 1014 AGCGCT AGC^GCT
EcoRV 124 GATATC GAT^ATC
EcoT14I 1952 CCATGG C^C[AT][AT]GG
HgaI 334 GACGC GACGC(5/10)
HincII 692 GTCGAC GT[CT]^[GA]AC
HindII 692 GTCGAC GT[CT]^[GA]AC
HindIII 346 AAGCTT A^AGCTT
NciI 1111 CCGGG CC^[GC]GG
NcoI 1952 CCATGG C^CATGG
NdeI 894 CATATG CA^TATG
PflMI 2510 CCAGATAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2485 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 1862 ATTAAT AT^TAAT
Psp1406I 1076 AACGTT AA^CGTT
RcaI 793 TCATGA T^CATGA
SacI 27 GAGCTC GAGCT^C
SalI 692 GTCGAC G^TCGAC
SmlI 62 CTTGAG C^T[CT][GA]AG
SpeI 19 ACTAGT A^CTAGT
SspBI 1818 TGTACA T^GTACA
StyI 1952 CCATGG C^C[AT][AT]GG
Van91I 2510 CCAGATAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 590 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 770 CGCG CG^CG
964 CGCG
AflIII 1000 ACATGT A^C[GA][CT]GT
1179 ACGTGT
AspI 1115 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1372 GACTGTGTC
BbsI 734 GAAGAC GAAGAC(2/6)
2568 GAAGAC
BcgI 2082 CGAAAGAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
954 GCACTCAGATCG
BpuAI 734 GAAGAC GAAGAC(2/6)
2568 GAAGAC
BsgI 1298 CTGCAC GTGCAG(16/14)
2105 CTGCAC
BsiYI 911 CCCAACACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2510 CCAGATAGTGG
BslI 911 CCCAACACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2510 CCAGATAGTGG
BstUI 770 CGCG CG^CG
964 CGCG
CfoI 1015 GCGC GCG^C
1315 GCGC
DsaI 1092 CCGTGG C^C[GA][CT]GG
1952 CCATGG
EcoRI 1717 GAATTC G^AATTC
2000 GAATTC
HaeII 1014 AGCGCT [GA]GCGC^[CT]
1314 GGCGCT
HapII 1111 CCGG C^CGG
2110 CCGG
HhaI 1015 GCGC GCG^C
1315 GCGC
HinP1I 1015 GCGC G^CGC
1315 GCGC
HpaII 1111 CCGG C^CGG
2110 CCGG
MspI 1111 CCGG C^CGG
2110 CCGG
MvnI 770 CGCG CG^CG
964 CGCG
PflFI 1115 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1372 GACTGTGTC
PleI 2227 GACTC GAGTC(4/5)
1119 GAGTC
PstI 1169 CTGCAG CTGCA^G
1250 CTGCAG
Tth111I 1115 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1372 GACTGTGTC
XcmI 45 CCACCGCTTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
804 CCATCACCTCAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanII BbvI
BfaI BmyI BpmI BsaJI BsiHKAI
BsmAI BsmFI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV NspI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII